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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AgsI | TTSAA | 1 cut(s) | 275 |
| Alw21I | GWGCWC | 1 cut(s) | 193 |
| Alw26I | GTCTC | 1 cut(s) | 150 |
| Alw44I | GTGCAC | 1 cut(s) | 189 |
| AoxI | GGCC | 1 cut(s) | 239 |
| ApaLI | GTGCAC | 1 cut(s) | 189 |
| AspS9I | GGNCC | 3 cut(s) | 65, 240, 280 |
| AvaII | GGWCC | 2 cut(s) | 65, 280 |
| BaeGI | GKGCMC | 2 cut(s) | 107, 193 |
| Bbv12I | GWGCWC | 1 cut(s) | 193 |
| BccI | CCATC | 2 cut(s) | 77, 100 |
| BclI | TGATCA | 1 cut(s) | 247 |
| BcoDI | GTCTC | 1 cut(s) | 150 |
| Bme18I | GGWCC | 2 cut(s) | 65, 280 |
| BmgT120I | GGNCC | 3 cut(s) | 65, 240, 280 |
| BmiI | GGNNCC | 1 cut(s) | 281 |
| BsaBI | GATNNNNATC | 2 cut(s) | 91, 249 |
| Bse8I | GATNNNNATC | 2 cut(s) | 91, 249 |
| BseGI | GGATG | 2 cut(s) | 92, 250 |
| BseJI | GATNNNNATC | 2 cut(s) | 91, 249 |
| BseSI | GKGCMC | 2 cut(s) | 107, 193 |
| BshFI | GGCC | 1 cut(s) | 241 |
| BsiHKAI | GWGCWC | 1 cut(s) | 193 |
| BsmAI | GTCTC | 1 cut(s) | 150 |
| BsmBI | CGTCTC | 1 cut(s) | 150 |
| BsnI | GGCC | 1 cut(s) | 241 |
| Bsp1286I | GDGCHC | 2 cut(s) | 107, 193 |
| Bsp143I | GATC | 1 cut(s) | 247 |
| BspANI | GGCC | 1 cut(s) | 241 |
| BspLI | GGNNCC | 1 cut(s) | 281 |
| BssMI | GATC | 1 cut(s) | 247 |
| Bst4CI | ACNGT | 1 cut(s) | 284 |
| BstF5I | GGATG | 2 cut(s) | 92, 250 |
| BstKTI | GATC | 1 cut(s) | 250 |
| BstMAI | GTCTC | 1 cut(s) | 150 |
| BstMBI | GATC | 1 cut(s) | 247 |
| BstSLI | GKGCMC | 2 cut(s) | 107, 193 |
| BsuRI | GGCC | 1 cut(s) | 241 |
| BtgZI | GCGATG | 1 cut(s) | 96 |
| BtsCI | GGATG | 2 cut(s) | 92, 250 |
| BtsI | GCAGTG | 1 cut(s) | 39 |
| BtsIMutI | CAGTG | 2 cut(s) | 39, 191 |
| Cfr13I | GGNCC | 3 cut(s) | 65, 240, 280 |
| CseI | GACGC | 1 cut(s) | 132 |
| CviAII | CATG | 2 cut(s) | 122, 260 |
| CviJI | RGCY | 3 cut(s) | 110, 164, 241 |
| CviKI_1 | RGCY | 3 cut(s) | 110, 164, 241 |
| DpnI | GATC | 1 cut(s) | 249 |
| DpnII | GATC | 1 cut(s) | 247 |
| Eco47I | GGWCC | 2 cut(s) | 65, 280 |
| Esp3I | CGTCTC | 1 cut(s) | 150 |
| FaeI | CATG | 2 cut(s) | 125, 263 |
| FaiI | YATR | 8 cut(s) | 15, 30, 63, 123, 128, 150, 204, 261 |
| FatI | CATG | 2 cut(s) | 121, 259 |
| FbaI | TGATCA | 1 cut(s) | 247 |
| FokI | GGATG | 2 cut(s) | 79, 237 |
| HaeIII | GGCC | 1 cut(s) | 241 |
| HgaI | GACGC | 1 cut(s) | 132 |
| Hin1II | CATG | 2 cut(s) | 125, 263 |
| HinfI | GANTC | 1 cut(s) | 181 |
| Hpy166II | GTNNAC | 1 cut(s) | 191 |
| Hpy8I | GTNNAC | 1 cut(s) | 191 |
| HpyAV | CCTTC | 2 cut(s) | 175, 278 |
| HpyCH4III | ACNGT | 1 cut(s) | 284 |
| HpyCH4V | TGCA | 3 cut(s) | 19, 191, 206 |
| Hsp92II | CATG | 2 cut(s) | 125, 263 |
| Ksp22I | TGATCA | 1 cut(s) | 247 |
| Kzo9I | GATC | 1 cut(s) | 247 |
| LmnI | GCTCC | 1 cut(s) | 37 |
| LpnPI | CCDG | 2 cut(s) | 48, 162 |
| MaeIII | GTNAC | 1 cut(s) | 37 |
| MalI | GATC | 1 cut(s) | 249 |
| MboI | GATC | 1 cut(s) | 247 |
| MboII | GAAGA | 3 cut(s) | 16, 163, 263 |
| MhlI | GDGCHC | 2 cut(s) | 107, 193 |
| MnlI | CCTC | 1 cut(s) | 107 |
| NdeII | GATC | 1 cut(s) | 247 |
| NlaIII | CATG | 2 cut(s) | 125, 263 |
| NlaIV | GGNNCC | 1 cut(s) | 281 |
| NmuCI | GTSAC | 1 cut(s) | 37 |
| PfeI | GAWTC | 1 cut(s) | 181 |
| PspN4I | GGNNCC | 1 cut(s) | 281 |
| PspPI | GGNCC | 3 cut(s) | 65, 240, 280 |
| Sau3AI | GATC | 1 cut(s) | 247 |
| Sau96I | GGNCC | 3 cut(s) | 65, 240, 280 |
| SduI | GDGCHC | 2 cut(s) | 107, 193 |
| SetI | ASST | 1 cut(s) | 57 |
| SgeI | CNNG | 9 cut(s) | 47, 64, 112, 114, 134, 189, 254, 267, 272 |
| SinI | GGWCC | 2 cut(s) | 65, 280 |
| TaaI | ACNGT | 1 cut(s) | 284 |
| TaqI | TCGA | 1 cut(s) | 24 |
| TfiI | GAWTC | 1 cut(s) | 181 |
| TscAI | CASTG | 2 cut(s) | 46, 198 |
| TseFI | GTSAC | 1 cut(s) | 37 |
| Tsp45I | GTSAC | 1 cut(s) | 37 |
| TspDTI | ATGAA | 3 cut(s) | 17, 157, 174 |
| TspGWI | ACGGA | 1 cut(s) | 243 |
| TspRI | CASTG | 2 cut(s) | 46, 198 |
| VneI | GTGCAC | 1 cut(s) | 189 |
| VpaK11BI | GGWCC | 2 cut(s) | 65, 280 |