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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 206 |
| AcsI | RAATTY | 1 cut(s) | 116 |
| AfaI | GTAC | 1 cut(s) | 5 |
| AgsI | TTSAA | 3 cut(s) | 44, 71, 139 |
| AluBI | AGCT | 5 cut(s) | 10, 100, 163, 253, 383 |
| AluI | AGCT | 5 cut(s) | 10, 100, 163, 253, 383 |
| Alw26I | GTCTC | 1 cut(s) | 311 |
| AoxI | GGCC | 1 cut(s) | 171 |
| ApaI | GGGCCC | 1 cut(s) | 175 |
| ApeKI | GCWGC | 2 cut(s) | 163, 380 |
| ApoI | RAATTY | 1 cut(s) | 116 |
| AspLEI | GCGC | 1 cut(s) | 94 |
| AspS9I | GGNCC | 2 cut(s) | 171, 172 |
| BaeGI | GKGCMC | 1 cut(s) | 175 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 251, 284 |
| BanII | GRGCYC | 1 cut(s) | 175 |
| BbsI | GAAGAC | 1 cut(s) | 228 |
| BbvI | GCAGC | 2 cut(s) | 150, 392 |
| BcoDI | GTCTC | 1 cut(s) | 311 |
| BfaI | CTAG | 1 cut(s) | 303 |
| BfmI | CTRYAG | 1 cut(s) | 164 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 23 |
| BisI | GCNGC | 2 cut(s) | 164, 381 |
| BlsI | GCNGC | 2 cut(s) | 165, 382 |
| BmgT120I | GGNCC | 2 cut(s) | 171, 172 |
| BmiI | GGNNCC | 2 cut(s) | 172, 173 |
| BmsI | GCATC | 1 cut(s) | 367 |
| BpiI | GAAGAC | 1 cut(s) | 228 |
| BseGI | GGATG | 1 cut(s) | 397 |
| BseRI | GAGGAG | 1 cut(s) | 299 |
| BseSI | GKGCMC | 1 cut(s) | 175 |
| BseXI | GCAGC | 2 cut(s) | 150, 392 |
| BshFI | GGCC | 1 cut(s) | 173 |
| BsmAI | GTCTC | 1 cut(s) | 311 |
| BsnI | GGCC | 1 cut(s) | 173 |
| Bsp120I | GGGCCC | 1 cut(s) | 171 |
| Bsp1286I | GDGCHC | 1 cut(s) | 175 |
| Bsp1407I | TGTACA | 1 cut(s) | 3 |
| Bsp143I | GATC | 1 cut(s) | 106 |
| BspANI | GGCC | 1 cut(s) | 173 |
| BspLI | GGNNCC | 2 cut(s) | 172, 173 |
| BspMAI | CTGCAG | 1 cut(s) | 168 |
| BsrGI | TGTACA | 1 cut(s) | 3 |
| BssMI | GATC | 1 cut(s) | 106 |
| Bst4CI | ACNGT | 1 cut(s) | 264 |
| BstAUI | TGTACA | 1 cut(s) | 3 |
| BstF5I | GGATG | 1 cut(s) | 397 |
| BstHHI | GCGC | 1 cut(s) | 94 |
| BstKTI | GATC | 1 cut(s) | 109 |
| BstMAI | GTCTC | 1 cut(s) | 311 |
| BstMBI | GATC | 1 cut(s) | 106 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 23 |
| BstSFI | CTRYAG | 1 cut(s) | 164 |
| BstSLI | GKGCMC | 1 cut(s) | 175 |
| BstV1I | GCAGC | 2 cut(s) | 150, 392 |
| BstV2I | GAAGAC | 1 cut(s) | 228 |
| BsuRI | GGCC | 1 cut(s) | 173 |
| BtsCI | GGATG | 1 cut(s) | 397 |
| BtsI | GCAGTG | 1 cut(s) | 33 |
| BtsIMutI | CAGTG | 2 cut(s) | 33, 128 |
| CfoI | GCGC | 1 cut(s) | 94 |
| Cfr13I | GGNCC | 2 cut(s) | 171, 172 |
| Csp6I | GTAC | 1 cut(s) | 4 |
| CviAII | CATG | 2 cut(s) | 272, 361 |
| CviQI | GTAC | 1 cut(s) | 4 |
| DpnI | GATC | 1 cut(s) | 108 |
| DpnII | GATC | 1 cut(s) | 106 |
| DraI | TTTAAA | 1 cut(s) | 81 |
| Eco24I | GRGCYC | 1 cut(s) | 175 |
| EcoO109I | RGGNCCY | 1 cut(s) | 171 |
| EcoT38I | GRGCYC | 1 cut(s) | 175 |
| FaeI | CATG | 2 cut(s) | 275, 364 |
| FaiI | YATR | 5 cut(s) | 206, 273, 284, 317, 362 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 283, 315 |
| FatI | CATG | 2 cut(s) | 271, 360 |
| Fnu4HI | GCNGC | 2 cut(s) | 164, 381 |
| FokI | GGATG | 1 cut(s) | 404 |
| FriOI | GRGCYC | 1 cut(s) | 175 |
| Fsp4HI | GCNGC | 2 cut(s) | 164, 381 |
| FspBI | CTAG | 1 cut(s) | 303 |
| GlaI | GCGC | 1 cut(s) | 93 |
| GluI | GCNGC | 2 cut(s) | 164, 381 |
| HaeIII | GGCC | 1 cut(s) | 173 |
| HhaI | GCGC | 1 cut(s) | 94 |
| Hin1II | CATG | 2 cut(s) | 275, 364 |
| Hin6I | GCGC | 1 cut(s) | 92 |
| HinP1I | GCGC | 1 cut(s) | 92 |
| Hpy188I | TCNGA | 3 cut(s) | 115, 249, 369 |
| HpyAV | CCTTC | 1 cut(s) | 82 |
| HpyCH4III | ACNGT | 1 cut(s) | 264 |
| HpyCH4V | TGCA | 3 cut(s) | 166, 201, 380 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 23 |
| Hsp92II | CATG | 2 cut(s) | 275, 364 |
| HspAI | GCGC | 1 cut(s) | 92 |
| Kzo9I | GATC | 1 cut(s) | 106 |
| LmnI | GCTCC | 3 cut(s) | 15, 97, 276 |
| LpnPI | CCDG | 4 cut(s) | 8, 62, 80, 152 |
| Lsp1109I | GCAGC | 2 cut(s) | 150, 392 |
| LweI | GCATC | 1 cut(s) | 367 |
| MaeI | CTAG | 1 cut(s) | 303 |
| MaeIII | GTNAC | 1 cut(s) | 394 |
| MalI | GATC | 1 cut(s) | 108 |
| MboI | GATC | 1 cut(s) | 106 |
| MboII | GAAGA | 3 cut(s) | 64, 67, 228 |
| MhlI | GDGCHC | 1 cut(s) | 175 |
| MluCI | AATT | 2 cut(s) | 116, 186 |
| MnlI | CCTC | 4 cut(s) | 28, 184, 250, 320 |
| MseI | TTAA | 5 cut(s) | 60, 80, 185, 290, 442 |
| MspA1I | CMGCKG | 1 cut(s) | 383 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 23 |
| NdeII | GATC | 1 cut(s) | 106 |
| NlaIII | CATG | 2 cut(s) | 275, 364 |
| NlaIV | GGNNCC | 2 cut(s) | 172, 173 |
| PkrI | GCNGC | 2 cut(s) | 165, 382 |
| PsiI | TTATAA | 1 cut(s) | 206 |
| PspN4I | GGNNCC | 2 cut(s) | 172, 173 |
| PspOMI | GGGCCC | 1 cut(s) | 171 |
| PspPI | GGNCC | 2 cut(s) | 171, 172 |
| PstI | CTGCAG | 1 cut(s) | 168 |
| PvuII | CAGCTG | 1 cut(s) | 383 |
| RsaI | GTAC | 1 cut(s) | 5 |
| RsaNI | GTAC | 1 cut(s) | 4 |
| SaqAI | TTAA | 5 cut(s) | 60, 80, 185, 290, 442 |
| SatI | GCNGC | 2 cut(s) | 164, 381 |
| Sau3AI | GATC | 1 cut(s) | 106 |
| Sau96I | GGNCC | 2 cut(s) | 171, 172 |
| SduI | GDGCHC | 1 cut(s) | 175 |
| SetI | ASST | 7 cut(s) | 12, 102, 165, 195, 255, 335, 385 |
| SfaNI | GCATC | 1 cut(s) | 367 |
| SfcI | CTRYAG | 1 cut(s) | 164 |
| Sse9I | AATT | 2 cut(s) | 116, 186 |
| SspMI | CTAG | 1 cut(s) | 303 |
| TaaI | ACNGT | 1 cut(s) | 264 |
| TaqI | TCGA | 1 cut(s) | 312 |
| TasI | AATT | 2 cut(s) | 116, 186 |
| TatI | WGTACW | 1 cut(s) | 3 |
| Tru1I | TTAA | 5 cut(s) | 60, 80, 185, 290, 442 |
| Tru9I | TTAA | 5 cut(s) | 60, 80, 185, 290, 442 |
| TscAI | CASTG | 2 cut(s) | 33, 135 |
| TseI | GCWGC | 2 cut(s) | 163, 380 |
| TspRI | CASTG | 2 cut(s) | 33, 135 |
| XapI | RAATTY | 1 cut(s) | 116 |
| XspI | CTAG | 1 cut(s) | 303 |