RchiOBHm_Chr2g0109351

Cysteine desulfurase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002608
Physical Location & Seq
Reverse (-)
20727863 .. 20728207
345 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 345 bp
ATGAAGGAGTTCAAGATTCCGTTTCATACTGATGCTGCTCAGGCATTGGGGAAGATTCCGATTGATGTGGATAAATGGGATGTGAGCTTGATGTCGTTGAGTGGCCACAAGGTGTATGGGCCTAAGGGAGTTGGAGCATTGTACATGAGGCGGCAGCCGAGGATTCGGGTGGAGCCTCGGATGAATGGAGGTGGCCAGGAGAGGGGGATAAGGAGCGGGACGGTTCCGACGTCGTTTGTGTTGGGGATGGGGACTGCTTGCGAGATTGCGAAGAAGCTTTATGAAACGGTGAAGGAAGGGATCAATATTAAGGATAATGTGGTCACAGCATTGAGCAGTGTGTGA

Protein Analysis

114

Amino Acids

12.45

Weight (kDa)

9.67

Isoelectric Point (pI)

30.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_5 PF00266 3 - 93 7.6e-20 Aminotransferase class-V
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 233
AccBSI CCGCTC 1 cut(s) 216
AciI CCGC 2 cut(s) 151, 216
AclWI GGATC 1 cut(s) 308
AcoI YGGCCR 2 cut(s) 103, 193
AcyI GRCGYC 1 cut(s) 230
AdeI CACNNNGTG 1 cut(s) 112
AfaI GTAC 1 cut(s) 143
AfiI CCNNNNNNNGG 1 cut(s) 202
AgsI TTSAA 1 cut(s) 13
AjnI CCWGG 1 cut(s) 195
AluBI AGCT 2 cut(s) 87, 277
AluI AGCT 2 cut(s) 87, 277
AlwI GGATC 1 cut(s) 308
AoxI GGCC 3 cut(s) 103, 119, 193
ApeKI GCWGC 2 cut(s) 35, 154
Asp700I GAANNNNTTC 1 cut(s) 8
AspS9I GGNCC 1 cut(s) 119
AsuHPI GGTGA 1 cut(s) 301
AxyI CCTNAGG 1 cut(s) 123
BalI TGGCCA 2 cut(s) 105, 195
BbvI GCAGC 2 cut(s) 22, 166
BccI CCATC 1 cut(s) 241
BciT130I CCWGG 1 cut(s) 197
BisI GCNGC 3 cut(s) 36, 152, 155
BlsI GCNGC 3 cut(s) 37, 153, 156
Bme1390I CCNGG 1 cut(s) 197
BmgT120I GGNCC 1 cut(s) 119
BmiI GGNNCC 2 cut(s) 174, 225
BmrFI CCNGG 1 cut(s) 197
BmsI GCATC 1 cut(s) 22
Bpu10I CCTNAGC 1 cut(s) 39
BsaHI GRCGYC 1 cut(s) 230
BsaJI CCNNGG 2 cut(s) 158, 176
Bsc4I CCNNNNNNNGG 1 cut(s) 202
Bse21I CCTNAGG 1 cut(s) 123
BseBI CCWGG 1 cut(s) 197
BseDI CCNNGG 2 cut(s) 158, 176
BseGI GGATG 3 cut(s) 85, 186, 252
BseLI CCNNNNNNNGG 1 cut(s) 202
BseMII CTCAG 1 cut(s) 53
BseXI GCAGC 2 cut(s) 22, 166
BshFI GGCC 3 cut(s) 105, 121, 195
BslFI GGGAC 2 cut(s) 232, 265
BslI CCNNNNNNNGG 1 cut(s) 202
BsmFI GGGAC 2 cut(s) 232, 265
BsnI GGCC 3 cut(s) 105, 121, 195
Bsp1407I TGTACA 1 cut(s) 141
Bsp143I GATC 1 cut(s) 300
BspACI CCGC 2 cut(s) 151, 216
BspANI GGCC 3 cut(s) 105, 121, 195
BspCNI CTCAG 1 cut(s) 52
BspLI GGNNCC 2 cut(s) 174, 225
BspPI GGATC 1 cut(s) 308
BsrBI CCGCTC 1 cut(s) 216
BsrGI TGTACA 1 cut(s) 141
BssECI CCNNGG 2 cut(s) 158, 176
BssMI GATC 1 cut(s) 300
BssNI GRCGYC 1 cut(s) 230
Bst2UI CCWGG 1 cut(s) 197
Bst4CI ACNGT 2 cut(s) 223, 289
BstACI GRCGYC 1 cut(s) 230
BstAUI TGTACA 1 cut(s) 141
BstC8I GCNNGC 1 cut(s) 259
BstDEI CTNAG 2 cut(s) 39, 123
BstF5I GGATG 3 cut(s) 85, 186, 252
BstKTI GATC 1 cut(s) 303
BstMBI GATC 1 cut(s) 300
BstMWI GCNNNNNNNGC 1 cut(s) 41
BstNI CCWGG 1 cut(s) 197
BstSCI CCNGG 1 cut(s) 195
BstV1I GCAGC 2 cut(s) 22, 166
Bsu36I CCTNAGG 1 cut(s) 123
BsuRI GGCC 3 cut(s) 105, 121, 195
BtsCI GGATG 3 cut(s) 85, 186, 252
BtsI GCAGTG 1 cut(s) 343
BtsIMutI CAGTG 1 cut(s) 343
Cac8I GCNNGC 1 cut(s) 259
Cfr13I GGNCC 1 cut(s) 119
Csp6I GTAC 1 cut(s) 142
CviAII CATG 1 cut(s) 145
CviJI RGCY 7 cut(s) 87, 105, 121, 157, 175, 195, 277
CviKI_1 RGCY 7 cut(s) 87, 105, 121, 157, 175, 195, 277
CviQI GTAC 1 cut(s) 142
DdeI CTNAG 2 cut(s) 39, 123
DpnI GATC 1 cut(s) 302
DpnII GATC 1 cut(s) 300
DraIII CACNNNGTG 1 cut(s) 112
EaeI YGGCCR 2 cut(s) 103, 193
Eco81I CCTNAGG 1 cut(s) 123
EcoRII CCWGG 1 cut(s) 195
FaeI CATG 1 cut(s) 148
FaiI YATR 4 cut(s) 27, 117, 146, 282
FaqI GGGAC 2 cut(s) 232, 265
FatI CATG 1 cut(s) 144
FauI CCCGC 1 cut(s) 209
Fnu4HI GCNGC 3 cut(s) 36, 152, 155
FokI GGATG 3 cut(s) 92, 193, 259
Fsp4HI GCNGC 3 cut(s) 36, 152, 155
GluI GCNGC 3 cut(s) 36, 152, 155
HaeIII GGCC 3 cut(s) 105, 121, 195
Hin1I GRCGYC 1 cut(s) 230
Hin1II CATG 1 cut(s) 148
HindIII AAGCTT 1 cut(s) 275
HinfI GANTC 3 cut(s) 16, 55, 163
HphI GGTGA 1 cut(s) 301
Hpy188I TCNGA 3 cut(s) 60, 180, 228
Hpy188III TCNNGA 1 cut(s) 13
Hpy99I CGWCG 2 cut(s) 232, 235
HpyAV CCTTC 2 cut(s) 286, 290
HpyCH4III ACNGT 2 cut(s) 223, 289
HpyCH4IV ACGT 1 cut(s) 230
HpyF10VI GCNNNNNNNGC 1 cut(s) 41
HpyF3I CTNAG 2 cut(s) 39, 123
HpySE526I ACGT 1 cut(s) 230
Hsp92I GRCGYC 1 cut(s) 230
Hsp92II CATG 1 cut(s) 148
Kzo9I GATC 1 cut(s) 300
LmnI GCTCC 3 cut(s) 134, 172, 213
LpnPI CCDG 3 cut(s) 26, 182, 209
Lsp1109I GCAGC 2 cut(s) 22, 166
LweI GCATC 1 cut(s) 22
MaeII ACGT 1 cut(s) 230
MaeIII GTNAC 1 cut(s) 322
MalI GATC 1 cut(s) 302
MbiI CCGCTC 1 cut(s) 216
MboI GATC 1 cut(s) 300
MboII GAAGA 2 cut(s) 64, 283
MlsI TGGCCA 2 cut(s) 105, 195
MluNI TGGCCA 2 cut(s) 105, 195
MmeI TCCRAC 2 cut(s) 112, 251
MnlI CCTC 5 cut(s) 141, 153, 182, 186, 195
Mox20I TGGCCA 2 cut(s) 105, 195
MroXI GAANNNNTTC 1 cut(s) 8
MscI TGGCCA 2 cut(s) 105, 195
MseI TTAA 1 cut(s) 309
MslI CAYNNNNRTG 1 cut(s) 30
Msp20I TGGCCA 2 cut(s) 105, 195
MspR9I CCNGG 1 cut(s) 197
MvaI CCWGG 1 cut(s) 197
MwoI GCNNNNNNNGC 1 cut(s) 41
NdeII GATC 1 cut(s) 300
NlaIII CATG 1 cut(s) 148
NlaIV GGNNCC 2 cut(s) 174, 225
NmeAIII GCCGAG 1 cut(s) 183
NmuCI GTSAC 1 cut(s) 322
PcsI WCGNNNNNNNCGW 1 cut(s) 227
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 3 cut(s) 16, 55, 163
PkrI GCNGC 3 cut(s) 37, 153, 156
Psp6I CCWGG 1 cut(s) 195
PspGI CCWGG 1 cut(s) 195
PspN4I GGNNCC 2 cut(s) 174, 225
PspPI GGNCC 1 cut(s) 119
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
RseI CAYNNNNRTG 1 cut(s) 30
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 3 cut(s) 36, 152, 155
Sau3AI GATC 1 cut(s) 300
Sau96I GGNCC 1 cut(s) 119
ScrFI CCNGG 1 cut(s) 197
SetI ASST 5 cut(s) 89, 114, 193, 233, 279
SfaNI GCATC 1 cut(s) 22
SmiMI CAYNNNNRTG 1 cut(s) 30
SsiI CCGC 2 cut(s) 151, 216
SspI AATATT 1 cut(s) 307
StyD4I CCNGG 1 cut(s) 195
TaaI ACNGT 2 cut(s) 223, 289
TaiI ACGT 1 cut(s) 233
TatI WGTACW 1 cut(s) 141
TauI GCSGC 1 cut(s) 154
TfiI GAWTC 3 cut(s) 16, 55, 163
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TscAI CASTG 1 cut(s) 343
TseFI GTSAC 1 cut(s) 322
TseI GCWGC 2 cut(s) 35, 154
Tsp45I GTSAC 1 cut(s) 322
TspDTI ATGAA 4 cut(s) 14, 17, 197, 297
TspGWI ACGGA 1 cut(s) 9
TspRI CASTG 1 cut(s) 343
XcmI CCANNNNNNNNNTGG 1 cut(s) 113
XmnI GAANNNNTTC 1 cut(s) 8
ZraI GACGTC 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.