RchiOBHm_Chr5g0045361

Cysteine desulfurase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002608
Physical Location & Seq
Reverse (-)
41289949 .. 41290500
552 bp
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UTR
Exon/CDS
Intron
PRQ32346

Sequence Viewer

Length: 552 bp
ATGGCAGTGAACAATGAAATCGGTGTGATTCAACCTATGGAGGAAATCGGAGATATCTGTAAGGAGTTCAAGATTCTGTTTCACACTGATGCTGCTCAGGCGTTAGGGAAGATTCTGATTGATGTGGATAGGTGGAATGTGAGCTTGACGTCGTTGAGTGGACACAAGGTGTATAGGCCTAAGGGAGTTGGGGCATTGTACATGGGGCGGCGGCCGAGGATTCGGGTGGAGCCTCAGATGAATGGAGGTGGGCAGGAGAGGGGGATAAGGAGCGGGATGGTTTCGACGCCGTTGGTTGTGGGGATGGGGACTGCTTGCGAGATTGCGAAGAAGGAGATGGAGTATGATCAGAAGAGGATTAGTCAGCTGCAGGAGAGGATGCTGAGTGGGATTAGGGAGCAGCTAGATGAGGTGGTGGTGAATGGCAGTATCGAGAGAAGGTACCCAAAGAGGATCCCATGTTCTTGTTGGTGCATGTTGAAGGTTTTGCAGGTTGGTTCTATATTTAATCCCGCTGGCACCAAACATGGGTCAAGGCAATCTATAGCATAA

Protein Analysis

183

Amino Acids

20.39

Weight (kDa)

9.32

Isoelectric Point (pI)

53.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_5 PF00266 1 - 152 5.7e-26 Aminotransferase class-V
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 152
Acc36I ACCTGC 1 cut(s) 481
Acc65I GGTACC 1 cut(s) 441
AccB1I GGYRCC 2 cut(s) 441, 518
AccBSI CCGCTC 1 cut(s) 273
AciI CCGC 4 cut(s) 208, 211, 273, 513
AclWI GGATC 2 cut(s) 448, 461
AcoI YGGCCR 1 cut(s) 212
AcyI GRCGYC 2 cut(s) 149, 287
AdeI CACNNNGTG 1 cut(s) 169
AfaI GTAC 2 cut(s) 200, 443
AfiI CCNNNNNNNGG 1 cut(s) 528
AgsI TTSAA 3 cut(s) 32, 70, 481
AloI GAACNNNNNNTCC 2 cut(s) 445, 477
AluBI AGCT 3 cut(s) 144, 367, 403
AluI AGCT 3 cut(s) 144, 367, 403
AlwI GGATC 2 cut(s) 448, 461
AoxI GGCC 2 cut(s) 176, 212
ApeKI GCWGC 3 cut(s) 92, 367, 400
Asp718I GGTACC 1 cut(s) 441
AsuHPI GGTGA 1 cut(s) 430
AxyI CCTNAGG 1 cut(s) 180
BamHI GGATCC 1 cut(s) 453
BanI GGYRCC 2 cut(s) 441, 518
BbvI GCAGC 3 cut(s) 79, 354, 412
BccI CCATC 3 cut(s) 271, 298, 331
BceAI ACGGC 1 cut(s) 274
BclI TGATCA 1 cut(s) 346
BfaI CTAG 1 cut(s) 404
BfmI CTRYAG 2 cut(s) 368, 543
BfuAI ACCTGC 1 cut(s) 481
BisI GCNGC 5 cut(s) 93, 209, 212, 368, 401
BlsI GCNGC 5 cut(s) 94, 210, 213, 369, 402
BmiI GGNNCC 4 cut(s) 231, 443, 455, 520
BmsI GCATC 2 cut(s) 79, 369
Bpu10I CCTNAGC 1 cut(s) 96
BsaHI GRCGYC 2 cut(s) 149, 287
BsaJI CCNNGG 1 cut(s) 215
BsaXI ACNNNNNCTCC 2 cut(s) 326, 356
Bsc4I CCNNNNNNNGG 1 cut(s) 528
Bse21I CCTNAGG 1 cut(s) 180
BseDI CCNNGG 1 cut(s) 215
BseGI GGATG 3 cut(s) 282, 309, 384
BseLI CCNNNNNNNGG 1 cut(s) 528
BseMII CTCAG 3 cut(s) 110, 248, 374
BseX3I CGGCCG 1 cut(s) 212
BseXI GCAGC 3 cut(s) 79, 354, 412
Bsh1285I CGRYCG 1 cut(s) 215
BshFI GGCC 2 cut(s) 178, 214
BshNI GGYRCC 2 cut(s) 441, 518
BsiEI CGRYCG 1 cut(s) 215
BslFI GGGAC 1 cut(s) 322
BslI CCNNNNNNNGG 1 cut(s) 528
BsmFI GGGAC 1 cut(s) 322
BsnI GGCC 2 cut(s) 178, 214
Bsp1407I TGTACA 1 cut(s) 198
Bsp143I GATC 2 cut(s) 346, 453
BspACI CCGC 4 cut(s) 208, 211, 273, 513
BspANI GGCC 2 cut(s) 178, 214
BspCNI CTCAG 3 cut(s) 109, 247, 375
BspLI GGNNCC 4 cut(s) 231, 443, 455, 520
BspMAI CTGCAG 1 cut(s) 372
BspMI ACCTGC 1 cut(s) 481
BspPI GGATC 2 cut(s) 448, 461
BspT107I GGYRCC 2 cut(s) 441, 518
BsrBI CCGCTC 1 cut(s) 273
BsrGI TGTACA 1 cut(s) 198
BssECI CCNNGG 1 cut(s) 215
BssMI GATC 2 cut(s) 346, 453
BssNI GRCGYC 2 cut(s) 149, 287
Bst6I CTCTTC 1 cut(s) 347
BstACI GRCGYC 2 cut(s) 149, 287
BstAUI TGTACA 1 cut(s) 198
BstC8I GCNNGC 2 cut(s) 316, 517
BstDEI CTNAG 4 cut(s) 96, 180, 234, 383
BstF5I GGATG 3 cut(s) 282, 309, 384
BstKTI GATC 2 cut(s) 349, 456
BstMBI GATC 2 cut(s) 346, 453
BstMCI CGRYCG 1 cut(s) 215
BstMWI GCNNNNNNNGC 1 cut(s) 98
BstNSI RCATGY 1 cut(s) 478
BstSFI CTRYAG 2 cut(s) 368, 543
BstV1I GCAGC 3 cut(s) 79, 354, 412
BstX2I RGATCY 1 cut(s) 453
BstYI RGATCY 1 cut(s) 453
BstZI CGGCCG 1 cut(s) 212
Bsu36I CCTNAGG 1 cut(s) 180
BsuRI GGCC 2 cut(s) 178, 214
BtsCI GGATG 3 cut(s) 282, 309, 384
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 2 cut(s) 12, 84
BveI ACCTGC 1 cut(s) 481
Cac8I GCNNGC 2 cut(s) 316, 517
CseI GACGC 1 cut(s) 295
Csp6I GTAC 2 cut(s) 199, 442
CviAII CATG 4 cut(s) 202, 459, 475, 527
CviJI RGCY 6 cut(s) 144, 178, 214, 232, 367, 403
CviKI_1 RGCY 6 cut(s) 144, 178, 214, 232, 367, 403
CviQI GTAC 2 cut(s) 199, 442
DdeI CTNAG 4 cut(s) 96, 180, 234, 383
DpnI GATC 2 cut(s) 348, 455
DpnII GATC 2 cut(s) 346, 453
DraIII CACNNNGTG 1 cut(s) 169
EaeI YGGCCR 1 cut(s) 212
EagI CGGCCG 1 cut(s) 212
Eam1104I CTCTTC 1 cut(s) 347
EarI CTCTTC 1 cut(s) 347
EclXI CGGCCG 1 cut(s) 212
Eco147I AGGCCT 1 cut(s) 178
Eco32I GATATC 1 cut(s) 55
Eco52I CGGCCG 1 cut(s) 212
Eco81I CCTNAGG 1 cut(s) 180
EcoRV GATATC 1 cut(s) 55
FaeI CATG 4 cut(s) 205, 462, 478, 530
FaqI GGGAC 1 cut(s) 322
FatI CATG 4 cut(s) 201, 458, 474, 526
FauI CCCGC 2 cut(s) 266, 520
FbaI TGATCA 1 cut(s) 346
Fnu4HI GCNGC 5 cut(s) 93, 209, 212, 368, 401
FokI GGATG 3 cut(s) 289, 316, 391
Fsp4HI GCNGC 5 cut(s) 93, 209, 212, 368, 401
FspBI CTAG 1 cut(s) 404
GluI GCNGC 5 cut(s) 93, 209, 212, 368, 401
HaeIII GGCC 2 cut(s) 178, 214
HgaI GACGC 1 cut(s) 295
Hin1I GRCGYC 2 cut(s) 149, 287
Hin1II CATG 4 cut(s) 205, 462, 478, 530
HinfI GANTC 4 cut(s) 28, 73, 112, 220
HphI GGTGA 1 cut(s) 430
Hpy166II GTNNAC 2 cut(s) 10, 161
Hpy188I TCNGA 4 cut(s) 50, 117, 237, 351
Hpy188III TCNNGA 2 cut(s) 70, 433
Hpy8I GTNNAC 2 cut(s) 10, 161
Hpy99I CGWCG 2 cut(s) 154, 289
HpyAV CCTTC 3 cut(s) 325, 432, 475
HpyCH4IV ACGT 1 cut(s) 149
HpyCH4V TGCA 3 cut(s) 370, 474, 490
HpyF10VI GCNNNNNNNGC 1 cut(s) 98
HpyF3I CTNAG 4 cut(s) 96, 180, 234, 383
HpySE526I ACGT 1 cut(s) 149
Hsp92I GRCGYC 2 cut(s) 149, 287
Hsp92II CATG 4 cut(s) 205, 462, 478, 530
KpnI GGTACC 1 cut(s) 445
Ksp22I TGATCA 1 cut(s) 346
Kzo9I GATC 2 cut(s) 346, 453
LmnI GCTCC 3 cut(s) 229, 270, 397
LpnPI CCDG 5 cut(s) 83, 239, 356, 476, 501
Lsp1109I GCAGC 3 cut(s) 79, 354, 412
LweI GCATC 2 cut(s) 79, 369
MaeI CTAG 1 cut(s) 404
MaeII ACGT 1 cut(s) 149
MalI GATC 2 cut(s) 348, 455
MbiI CCGCTC 1 cut(s) 273
MboI GATC 2 cut(s) 346, 453
MboII GAAGA 3 cut(s) 121, 340, 364
MflI RGATCY 1 cut(s) 453
MnlI CCTC 9 cut(s) 34, 210, 239, 243, 252, 348, 369, 403, 444
MseI TTAA 1 cut(s) 507
MslI CAYNNNNRTG 1 cut(s) 87
MspA1I CMGCKG 2 cut(s) 367, 515
MwoI GCNNNNNNNGC 1 cut(s) 98
NdeII GATC 2 cut(s) 346, 453
NlaIII CATG 4 cut(s) 205, 462, 478, 530
NlaIV GGNNCC 4 cut(s) 231, 443, 455, 520
NmeAIII GCCGAG 1 cut(s) 240
NspI RCATGY 1 cut(s) 478
PceI AGGCCT 1 cut(s) 178
PfeI GAWTC 4 cut(s) 28, 73, 112, 220
PkrI GCNGC 5 cut(s) 94, 210, 213, 369, 402
PspN4I GGNNCC 4 cut(s) 231, 443, 455, 520
PstI CTGCAG 1 cut(s) 372
PsuI RGATCY 1 cut(s) 453
PvuII CAGCTG 1 cut(s) 367
RsaI GTAC 2 cut(s) 200, 443
RsaNI GTAC 2 cut(s) 199, 442
RseI CAYNNNNRTG 1 cut(s) 87
SaqAI TTAA 1 cut(s) 507
SatI GCNGC 5 cut(s) 93, 209, 212, 368, 401
Sau3AI GATC 2 cut(s) 346, 453
SfaNI GCATC 2 cut(s) 79, 369
SfcI CTRYAG 2 cut(s) 368, 543
SmiMI CAYNNNNRTG 1 cut(s) 87
SseBI AGGCCT 1 cut(s) 178
SsiI CCGC 4 cut(s) 208, 211, 273, 513
SspMI CTAG 1 cut(s) 404
StuI AGGCCT 1 cut(s) 178
TaiI ACGT 1 cut(s) 152
TaqI TCGA 2 cut(s) 284, 432
TatI WGTACW 1 cut(s) 198
TauI GCSGC 2 cut(s) 211, 214
TfiI GAWTC 4 cut(s) 28, 73, 112, 220
Tru1I TTAA 1 cut(s) 507
Tru9I TTAA 1 cut(s) 507
TscAI CASTG 2 cut(s) 12, 91
TseI GCWGC 3 cut(s) 92, 367, 400
TspDTI ATGAA 2 cut(s) 30, 254
TspRI CASTG 2 cut(s) 12, 91
XceI RCATGY 1 cut(s) 478
XcmI CCANNNNNNNNNTGG 1 cut(s) 465
XspI CTAG 1 cut(s) 404
ZraI GACGTC 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.