Rh4BG181900

Cysteine desulfurase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0002608
Physical Location & Seq
Forward (+)
32063551 .. 32064688
1138 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG181900.1

Sequence Viewer

Length: 1038 bp
ATGATAAGCAATCACAAGGATAAATATGAACGTGCCAAGAACATTGGCTATTATGCCAAATTGGTGATCACCAATCATCTTGGGTGGTGTTTGTTACACGGGATTGGACTGGATTGGACATTCTTATCTAGTCCAATCCCAGTTATCCCATGTTTGGCAGCAAGGGGACTCTTTTTAATGAGACTCCACAGTCCCAAAAACCACCCCGACACCGTCTACCACAAACCCACCAAAAACCATGGGATTGTGGAAGCCACTCTCACTCGATCTACTCTTCCCTTCCGTTCGTCGTTCTCTCATCATTCTCAACCTGCAATTCAACCTCAACCCATCATTCTCAGAAAGGATCCCGAAATTGAGCCCTCAGCAGTGCGATTCTCTCTCCACACCCACCAAACCTTCATCACCACCCAGACGGAGCACAAGTGCGTCCTTGACTCCTGCCGCCATCTCCAGCAGGAGGGTTTCGACATCACCTACCTTCCGGTGAAGCCCGACGGGCTCATCGACCTGGACCAGCTCCGGGCTGGATCCCGACCCGATACTGAACTGGTCTCAGTTATGGCAGTGAACAATGAAATCGGTGTGATTCAACCTATGGAGGAAATCGGAGATATTTGCAAGGAGTTCAAGATTCCGTTTCACACTGATGTTGCTCAAGTGTTAGGGAAGATTCCGATTGATGTGGATAGGTGGAATGTGAGCTTGATGTCGTTGACTGAGCACAAGGTGTATGGGCCCAAGGGAGTTGGGGCATTGTACATGAGGCGGCAGCCGAGGATTCGGGTGGAGCCTCAGATGAATGGAGGTGGGCAGGAGAGGGGGATAAGGAGCGGGACGGTTCCGACGCCGTTGGTTGTGGGGATGGGGACTGCTTGCGAGATTGCGAAGAAGGAGATGGAGTATGATCAGAAGAGGATTAGTCAGTTGCAGGAGAGGATGCTGAGTGGGATTAGGGAGCAGCTGGATGAGGTGGTGGTGAATGGCAGTATCGAGAGAAGGTACCCGGAGAGGATCCCATGTTCTTGCTGGTGTTGA

Protein Analysis

345

Amino Acids

39.05

Weight (kDa)

8.32

Isoelectric Point (pI)

54.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_5 PF00266 127 - 337 3.4e-44 Aminotransferase class-V
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 189
Acc36I ACCTGC 1 cut(s) 319
Acc65I GGTACC 1 cut(s) 1002
AccB1I GGYRCC 1 cut(s) 1002
AccBSI CCGCTC 1 cut(s) 834
AccI GTMKAC 1 cut(s) 216
AciI CCGC 3 cut(s) 445, 769, 834
AclWI GGATC 6 cut(s) 341, 354, 525, 538, 1009, 1022
AcyI GRCGYC 1 cut(s) 848
AdeI CACNNNGTG 1 cut(s) 730
AfaI GTAC 2 cut(s) 761, 1004
AfiI CCNNNNNNNGG 3 cut(s) 154, 460, 523
AgsI TTSAA 3 cut(s) 320, 593, 631
AjnI CCWGG 1 cut(s) 510
AloI GAACNNNNNNTCC 2 cut(s) 1006, 1038
AluBI AGCT 3 cut(s) 520, 705, 964
AluI AGCT 3 cut(s) 520, 705, 964
Alw21I GWGCWC 2 cut(s) 423, 726
Alw26I GTCTC 2 cut(s) 175, 559
AlwI GGATC 6 cut(s) 341, 354, 525, 538, 1009, 1022
AoxI GGCC 1 cut(s) 737
ApaI GGGCCC 1 cut(s) 741
ApeKI GCWGC 3 cut(s) 158, 772, 961
Asp718I GGTACC 1 cut(s) 1002
AspS9I GGNCC 3 cut(s) 514, 737, 738
AsuC2I CCSGG 2 cut(s) 524, 1007
AsuHPI GGTGA 6 cut(s) 61, 76, 397, 466, 499, 991
AvaII GGWCC 1 cut(s) 514
BaeGI GKGCMC 1 cut(s) 741
BamHI GGATCC 3 cut(s) 346, 530, 1014
BanI GGYRCC 1 cut(s) 1002
BanII GRGCYC 3 cut(s) 363, 504, 741
Bbv12I GWGCWC 2 cut(s) 423, 726
BbvCI CCTCAGC 1 cut(s) 364
BbvI GCAGC 3 cut(s) 170, 784, 973
BccI CCATC 4 cut(s) 338, 456, 859, 892
BceAI ACGGC 1 cut(s) 835
BciT130I CCWGG 1 cut(s) 512
BclI TGATCA 2 cut(s) 66, 907
BcnI CCSGG 2 cut(s) 524, 1007
BcoDI GTCTC 2 cut(s) 175, 559
BfaI CTAG 1 cut(s) 129
BfuAI ACCTGC 1 cut(s) 319
BglI GCCNNNNNGGC 1 cut(s) 499
BisI GCNGC 5 cut(s) 159, 445, 770, 773, 962
BlsI GCNGC 5 cut(s) 160, 446, 771, 774, 963
Bme1390I CCNGG 3 cut(s) 512, 524, 1007
Bme18I GGWCC 1 cut(s) 514
BmgT120I GGNCC 3 cut(s) 514, 737, 738
BmiI GGNNCC 7 cut(s) 348, 532, 739, 792, 843, 1004, 1016
BmrFI CCNGG 3 cut(s) 512, 524, 1007
BmrI ACTGGG 1 cut(s) 134
BmsI GCATC 1 cut(s) 930
BmuI ACTGGG 1 cut(s) 134
BpmI CTGGAG 1 cut(s) 437
Bpu10I CCTNAGC 1 cut(s) 364
BpuEI CTTGAG 1 cut(s) 642
BpuMI CCSGG 2 cut(s) 524, 1007
BsaHI GRCGYC 1 cut(s) 848
BsaI GGTCTC 1 cut(s) 559
BsaJI CCNNGG 3 cut(s) 238, 741, 776
BsaWI WCCGGW 1 cut(s) 484
BsaXI ACNNNNNCTCC 4 cut(s) 410, 440, 887, 917
Bsc4I CCNNNNNNNGG 3 cut(s) 154, 460, 523
Bse1I ACTGG 3 cut(s) 114, 140, 555
BseBI CCWGG 1 cut(s) 512
BseDI CCNNGG 3 cut(s) 238, 741, 776
BseGI GGATG 3 cut(s) 870, 945, 973
BseLI CCNNNNNNNGG 3 cut(s) 154, 460, 523
BseMII CTCAG 6 cut(s) 352, 378, 570, 711, 809, 935
BseNI ACTGG 3 cut(s) 114, 140, 555
BseSI GKGCMC 1 cut(s) 741
BseXI GCAGC 3 cut(s) 170, 784, 973
BshFI GGCC 1 cut(s) 739
BshNI GGYRCC 1 cut(s) 1002
BsiHKAI GWGCWC 2 cut(s) 423, 726
BsiSI CCGG 3 cut(s) 485, 523, 1007
BslFI GGGAC 4 cut(s) 177, 180, 850, 883
BslI CCNNNNNNNGG 3 cut(s) 154, 460, 523
BsmAI GTCTC 2 cut(s) 175, 559
BsmFI GGGAC 4 cut(s) 177, 180, 850, 883
BsnI GGCC 1 cut(s) 739
Bso31I GGTCTC 1 cut(s) 559
Bsp120I GGGCCC 1 cut(s) 737
Bsp1286I GDGCHC 5 cut(s) 363, 423, 504, 726, 741
Bsp1407I TGTACA 1 cut(s) 759
Bsp143I GATC 6 cut(s) 66, 266, 346, 530, 907, 1014
Bsp19I CCATGG 1 cut(s) 238
BspACI CCGC 3 cut(s) 445, 769, 834
BspANI GGCC 1 cut(s) 739
BspCNI CTCAG 6 cut(s) 351, 377, 569, 712, 808, 936
BspLI GGNNCC 7 cut(s) 348, 532, 739, 792, 843, 1004, 1016
BspMI ACCTGC 1 cut(s) 319
BspPI GGATC 6 cut(s) 341, 354, 525, 538, 1009, 1022
BspT107I GGYRCC 1 cut(s) 1002
BspTNI GGTCTC 1 cut(s) 559
BsrBI CCGCTC 1 cut(s) 834
BsrGI TGTACA 1 cut(s) 759
BsrI ACTGG 3 cut(s) 114, 140, 555
BssECI CCNNGG 3 cut(s) 238, 741, 776
BssMI GATC 6 cut(s) 66, 266, 346, 530, 907, 1014
BssNI GRCGYC 1 cut(s) 848
BssT1I CCWWGG 2 cut(s) 238, 741
Bst2UI CCWGG 1 cut(s) 512
Bst4CI ACNGT 3 cut(s) 191, 214, 841
Bst6I CTCTTC 2 cut(s) 279, 908
BstACI GRCGYC 1 cut(s) 848
BstAUI TGTACA 1 cut(s) 759
BstC8I GCNNGC 1 cut(s) 877
BstDEI CTNAG 6 cut(s) 338, 364, 556, 720, 795, 944
BstDSI CCRYGG 1 cut(s) 238
BstF5I GGATG 3 cut(s) 870, 945, 973
BstKTI GATC 6 cut(s) 69, 269, 349, 533, 910, 1017
BstMAI GTCTC 2 cut(s) 175, 559
BstMBI GATC 6 cut(s) 66, 266, 346, 530, 907, 1014
BstMWI GCNNNNNNNGC 1 cut(s) 499
BstNI CCWGG 1 cut(s) 512
BstSCI CCNGG 3 cut(s) 510, 522, 1005
BstSLI GKGCMC 1 cut(s) 741
BstV1I GCAGC 3 cut(s) 170, 784, 973
BstX2I RGATCY 3 cut(s) 346, 530, 1014
BstYI RGATCY 3 cut(s) 346, 530, 1014
BsuRI GGCC 1 cut(s) 739
BtgI CCRYGG 1 cut(s) 238
BtsCI GGATG 3 cut(s) 870, 945, 973
BtsI GCAGTG 2 cut(s) 375, 573
BtsIMutI CAGTG 3 cut(s) 375, 573, 645
BveI ACCTGC 1 cut(s) 319
Cac8I GCNNGC 1 cut(s) 877
Cfr13I GGNCC 3 cut(s) 514, 737, 738
CseI GACGC 2 cut(s) 418, 856
Csp6I GTAC 2 cut(s) 760, 1003
CviAII CATG 4 cut(s) 150, 239, 763, 1020
CviQI GTAC 2 cut(s) 760, 1003
DdeI CTNAG 6 cut(s) 338, 364, 556, 720, 795, 944
DpnI GATC 6 cut(s) 68, 268, 348, 532, 909, 1016
DpnII GATC 6 cut(s) 66, 266, 346, 530, 907, 1014
DraIII CACNNNGTG 1 cut(s) 730
DrdI GACNNNNNNGTC 1 cut(s) 189
DseDI GACNNNNNNGTC 1 cut(s) 189
Eam1104I CTCTTC 2 cut(s) 279, 908
EarI CTCTTC 2 cut(s) 279, 908
Eco130I CCWWGG 2 cut(s) 238, 741
Eco24I GRGCYC 3 cut(s) 363, 504, 741
Eco31I GGTCTC 1 cut(s) 559
Eco47I GGWCC 1 cut(s) 514
EcoRII CCWGG 1 cut(s) 510
EcoT14I CCWWGG 2 cut(s) 238, 741
EcoT38I GRGCYC 3 cut(s) 363, 504, 741
ErhI CCWWGG 2 cut(s) 238, 741
FaeI CATG 4 cut(s) 153, 242, 766, 1023
FaqI GGGAC 4 cut(s) 177, 180, 850, 883
FatI CATG 4 cut(s) 149, 238, 762, 1019
FauI CCCGC 1 cut(s) 827
FbaI TGATCA 2 cut(s) 66, 907
FblI GTMKAC 1 cut(s) 216
Fnu4HI GCNGC 5 cut(s) 159, 445, 770, 773, 962
FokI GGATG 3 cut(s) 877, 952, 980
FriOI GRGCYC 3 cut(s) 363, 504, 741
Fsp4HI GCNGC 5 cut(s) 159, 445, 770, 773, 962
FspBI CTAG 1 cut(s) 129
GluI GCNGC 5 cut(s) 159, 445, 770, 773, 962
GsuI CTGGAG 1 cut(s) 437
HaeIII GGCC 1 cut(s) 739
HapII CCGG 3 cut(s) 485, 523, 1007
HgaI GACGC 2 cut(s) 418, 856
Hin1I GRCGYC 1 cut(s) 848
Hin1II CATG 4 cut(s) 153, 242, 766, 1023
HincII GTYRAC 1 cut(s) 717
HindII GTYRAC 1 cut(s) 717
HinfI GANTC 8 cut(s) 168, 183, 375, 437, 589, 634, 673, 781
HpaII CCGG 3 cut(s) 485, 523, 1007
HphI GGTGA 6 cut(s) 61, 76, 397, 466, 499, 991
Hpy166II GTNNAC 3 cut(s) 217, 571, 717
Hpy188I TCNGA 6 cut(s) 341, 611, 678, 798, 846, 912
Hpy188III TCNNGA 4 cut(s) 350, 534, 631, 994
Hpy8I GTNNAC 3 cut(s) 217, 571, 717
Hpy99I CGWCG 3 cut(s) 292, 500, 850
HpyAV CCTTC 5 cut(s) 289, 409, 491, 886, 993
HpyCH4III ACNGT 3 cut(s) 191, 214, 841
HpyCH4IV ACGT 1 cut(s) 31
HpyCH4V TGCA 3 cut(s) 314, 621, 931
HpyF10VI GCNNNNNNNGC 1 cut(s) 499
HpyF3I CTNAG 6 cut(s) 338, 364, 556, 720, 795, 944
HpySE526I ACGT 1 cut(s) 31
Hsp92I GRCGYC 1 cut(s) 848
Hsp92II CATG 4 cut(s) 153, 242, 766, 1023
KpnI GGTACC 1 cut(s) 1006
Ksp22I TGATCA 2 cut(s) 66, 907
Kzo9I GATC 6 cut(s) 66, 266, 346, 530, 907, 1014
LmnI GCTCC 5 cut(s) 418, 525, 790, 831, 958
Lsp1109I GCAGC 3 cut(s) 170, 784, 973
LweI GCATC 1 cut(s) 930
MaeI CTAG 1 cut(s) 129
MaeII ACGT 1 cut(s) 31
MaeIII GTNAC 1 cut(s) 93
MalI GATC 6 cut(s) 68, 268, 348, 532, 909, 1016
MbiI CCGCTC 1 cut(s) 834
MboI GATC 6 cut(s) 66, 266, 346, 530, 907, 1014
MboII GAAGA 4 cut(s) 266, 682, 901, 925
MflI RGATCY 3 cut(s) 346, 530, 1014
MhlI GDGCHC 5 cut(s) 363, 423, 504, 726, 741
MluCI AATT 3 cut(s) 59, 315, 354
MlyI GAGTC 3 cut(s) 162, 177, 431
MmeI TCCRAC 1 cut(s) 869
MseI TTAA 1 cut(s) 176
MslI CAYNNNNRTG 1 cut(s) 648
MspA1I CMGCKG 1 cut(s) 964
MspI CCGG 3 cut(s) 485, 523, 1007
MspR9I CCNGG 3 cut(s) 512, 524, 1007
MvaI CCWGG 1 cut(s) 512
MwoI GCNNNNNNNGC 1 cut(s) 499
NciI CCSGG 2 cut(s) 524, 1007
NcoI CCATGG 1 cut(s) 238
NdeII GATC 6 cut(s) 66, 266, 346, 530, 907, 1014
NlaIII CATG 4 cut(s) 153, 242, 766, 1023
NlaIV GGNNCC 7 cut(s) 348, 532, 739, 792, 843, 1004, 1016
NmeAIII GCCGAG 1 cut(s) 801
PcsI WCGNNNNNNNCGW 1 cut(s) 504
PfeI GAWTC 5 cut(s) 375, 589, 634, 673, 781
PflFI GACNNNGTC 1 cut(s) 212
PkrI GCNGC 5 cut(s) 160, 446, 771, 774, 963
PleI GAGTC 3 cut(s) 162, 177, 431
PpsI GAGTC 3 cut(s) 162, 177, 431
Psp6I CCWGG 1 cut(s) 510
PspGI CCWGG 1 cut(s) 510
PspN4I GGNNCC 7 cut(s) 348, 532, 739, 792, 843, 1004, 1016
PspOMI GGGCCC 1 cut(s) 737
PspPI GGNCC 3 cut(s) 514, 737, 738
PsuI RGATCY 3 cut(s) 346, 530, 1014
PsyI GACNNNGTC 1 cut(s) 212
PvuII CAGCTG 1 cut(s) 964
RsaI GTAC 2 cut(s) 761, 1004
RsaNI GTAC 2 cut(s) 760, 1003
RseI CAYNNNNRTG 1 cut(s) 648
SaqAI TTAA 1 cut(s) 176
SatI GCNGC 5 cut(s) 159, 445, 770, 773, 962
Sau3AI GATC 6 cut(s) 66, 266, 346, 530, 907, 1014
Sau96I GGNCC 3 cut(s) 514, 737, 738
SchI GAGTC 3 cut(s) 162, 177, 431
ScrFI CCNGG 3 cut(s) 512, 524, 1007
SduI GDGCHC 5 cut(s) 363, 423, 504, 726, 741
SfaNI GCATC 1 cut(s) 930
SinI GGWCC 1 cut(s) 514
SmiMI CAYNNNNRTG 1 cut(s) 648
SmlI CTYRAG 1 cut(s) 657
SmoI CTYRAG 1 cut(s) 657
Sse9I AATT 3 cut(s) 59, 315, 354
SsiI CCGC 3 cut(s) 445, 769, 834
SspMI CTAG 1 cut(s) 129
StyD4I CCNGG 3 cut(s) 510, 522, 1005
StyI CCWWGG 2 cut(s) 238, 741
TaaI ACNGT 3 cut(s) 191, 214, 841
TaiI ACGT 1 cut(s) 34
TaqI TCGA 4 cut(s) 265, 468, 507, 993
TasI AATT 3 cut(s) 59, 315, 354
TatI WGTACW 1 cut(s) 759
TauI GCSGC 2 cut(s) 447, 772
TfiI GAWTC 5 cut(s) 375, 589, 634, 673, 781
Tru1I TTAA 1 cut(s) 176
Tru9I TTAA 1 cut(s) 176
TscAI CASTG 3 cut(s) 375, 573, 652
TseI GCWGC 3 cut(s) 158, 772, 961
TspDTI ATGAA 4 cut(s) 42, 391, 591, 815
TspGWI ACGGA 3 cut(s) 272, 431, 627
TspRI CASTG 3 cut(s) 375, 573, 652
Tth111I GACNNNGTC 1 cut(s) 212
VpaK11BI GGWCC 1 cut(s) 514
XcmI CCANNNNNNNNNTGG 2 cut(s) 524, 1026
XmiI GTMKAC 1 cut(s) 216
XspI CTAG 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.