RchiOBHm_Chr2g0145651

Mitochondrial ubiquitin ligase activator of NFKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002439
Physical Location & Seq
Reverse (-)
63374398 .. 63377270
2873 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1176 bp
ATGTCGTCGAACGAGCATACCCTAGCCTCCCTAATCTCCCAGCTCGTCCTCTCCTTAGACGGCGCCGTTTTGGGCACCATCGTCGCCTTCGCCGCCGTCGATTCCTTCCTCAACTTCAAATCCACCACCTCCGCCCTCCGCAAGATCCGCCAAGCCCCCTCCGTCAAAGTCTCCGATCTCCGCTCCCTTGTCCACGAAGACTCCGACCAACCGCAGCCTTCTGATGCCTCCAAGCTTGTCGTCCTCCGCGGCCGCGTCCAAGCCACACCCGACGCCGACGGAACCTACAAGAGCCTCAAGTCCAGCCCGATCATCAACCCCGATAATAACGAGAACACCACCATCGACGCCGTCATCGTTCAGAGGACGCAACGGCTTTTGTATAACGAGTGGAAGGGTTTTCTTTGGCGGACTTATGATTTGAGAGCTAAATTGGCGAAGCCTTGGAGAGAACAAGAATCCAGCACGATCAAAATGGTTCCTTTTGTTCTTGGTGAAGCTGGGAATTCCGATTTCGTTGTTCCGAATATCGATGGCTCAGGACACCCCTTGCCTCTTGTGACAGTTTATCAGCAAAAGCAGCTCTTTAATCCTAGTCCCTTTACATTCCTGCAGAAAATTTTTGGTCATGATTACCCAATCGGTCTACAAGATGAAGAAAAGGTTCTTCCATTGGGAGTCGAAGTCAGTGCTGTTGGTATCTGCAGTTTCCAAAATGGGATACCAGAAATCAAGTCATGCCGTGATCTTCCCTATTTTATGTCTTTGATGGATAAGGATGAGTTGGTTCTACATCTCGAACACAAGACAAACACATTATTCTGGTTTGGGGTTGGTTTTGGTTTGTTGTCAGTTGGAATCCTCTGCTATGCTGGTGCAAGGAATTGGCACAAATTTAGAGAATGGAGGCAACGAAGGCAGCCACAGCAAGCAAGCGATGCAGCTGGAACCATTGCTGATCCTCAAATTGAAGCAGAAGATGAGGAAGTAGGGGAAGTACCGGATGGGCAGTTATGTGTCATCTGTCTGATGAGGAGAAGACGATCTGCCTTCATTCCGTGCGGGCATCTTGTGTGTTGCCATCTCTGCTGCATATCCATTGAGCAGAGCACGTCACCCAAGTGCCCGGTCTGCAGGCAGGAGATCCGCACTGCAATGCGCATATATGACTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000266 GO:0001558 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005777 GO:0006464 GO:0006807 GO:0006919 GO:0006996 GO:0007005 GO:0008150 GO:0008152 GO:0009507 GO:0009526 GO:0009536 GO:0009893 GO:0009941 GO:0009966 GO:0009967 GO:0009987 GO:0010604 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0010950 GO:0010952 GO:0016020 GO:0016021 GO:0016043 GO:0016567 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019867 GO:0023051 GO:0023056 GO:0030162 GO:0030308 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031306 GO:0031307 GO:0031323 GO:0031325 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0032268 GO:0032270 GO:0032446 GO:0032592 GO:0036211 GO:0040008 GO:0042579 GO:0042802 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043085 GO:0043122 GO:0043123 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043280 GO:0043281 GO:0043412 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045862 GO:0045926 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0051128 GO:0051171 GO:0051173 GO:0051179 GO:0051246 GO:0051247 GO:0051336 GO:0051345 GO:0051640 GO:0051641 GO:0051646 GO:0052547 GO:0052548 GO:0060255 GO:0065007 GO:0065009 GO:0070647 GO:0071704 GO:0071840 GO:0080090 GO:0098573 GO:0098588 GO:0098805 GO:0140096 GO:1901564 GO:1902531 GO:1902533 GO:2000116 GO:2001056
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GIDE zf-C3HC4_3

Protein Analysis

391

Amino Acids

43.7

Weight (kDa)

6.32

Isoelectric Point (pI)

50.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIDE PF12483 148 - 286 6.5e-29 E3 Ubiquitin ligase
zf-C3HC4_3 PF13920 336 - 384 3.2e-12 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1160
AccB1I GGYRCC 2 cut(s) 62, 74
AccBSI CCGCTC 1 cut(s) 183
AccI GTMKAC 1 cut(s) 646
AccII CGCG 2 cut(s) 249, 255
AclWI GGATC 3 cut(s) 139, 953, 1138
AcoI YGGCCR 1 cut(s) 250
AcsI RAATTY 3 cut(s) 505, 618, 893
AcyI GRCGYC 3 cut(s) 63, 273, 348
AfaI GTAC 1 cut(s) 999
AgsI TTSAA 2 cut(s) 118, 971
AjiI CACGTC 1 cut(s) 1113
AleI CACNNNNGTG 1 cut(s) 1120
AluBI AGCT 6 cut(s) 43, 235, 428, 500, 583, 944
AluI AGCT 6 cut(s) 43, 235, 428, 500, 583, 944
Alw21I GWGCWC 1 cut(s) 1112
Alw26I GTCTC 1 cut(s) 175
AlwI GGATC 3 cut(s) 139, 953, 1138
AoxI GGCC 1 cut(s) 250
ApeKI GCWGC 5 cut(s) 214, 580, 919, 941, 1089
ApoI RAATTY 3 cut(s) 505, 618, 893
ArsI GACNNNNNNTTYG 2 cut(s) 403, 435
Asp700I GAANNNNTTC 1 cut(s) 663
AspLEI GCGC 2 cut(s) 65, 1161
AsuC2I CCSGG 1 cut(s) 1127
AsuHPI GGTGA 2 cut(s) 506, 1107
BaeGI GKGCMC 2 cut(s) 77, 1127
BanI GGYRCC 2 cut(s) 62, 74
BbsI GAAGAC 2 cut(s) 204, 1045
Bbv12I GWGCWC 1 cut(s) 1112
BbvI GCAGC 5 cut(s) 226, 592, 931, 953, 1076
BccI CCATC 6 cut(s) 86, 350, 527, 763, 998, 1089
BceAI ACGGC 6 cut(s) 50, 76, 80, 335, 389, 726
BcgI CGANNNNNNTGC 4 cut(s) 64, 98, 671, 705
BciVI GTATCC 1 cut(s) 714
BcnI CCSGG 1 cut(s) 1127
BcoDI GTCTC 1 cut(s) 175
BfaI CTAG 2 cut(s) 23, 594
BfmI CTRYAG 3 cut(s) 611, 703, 1132
BfoI RGCGCY 1 cut(s) 66
BfuI GTATCC 1 cut(s) 714
BisI GCNGC 8 cut(s) 93, 215, 250, 253, 581, 920, 942, 1090
BlsI GCNGC 8 cut(s) 94, 216, 251, 254, 582, 921, 943, 1091
Bme1390I CCNGG 1 cut(s) 1127
BmgBI CACGTC 1 cut(s) 1113
BmiI GGNNCC 5 cut(s) 64, 76, 283, 480, 949
BmrFI CCNGG 1 cut(s) 1127
BmsI GCATC 3 cut(s) 214, 928, 1075
BpiI GAAGAC 2 cut(s) 204, 1045
Bpu10I CCTNAGC 1 cut(s) 538
BpuEI CTTGAG 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 1127
Bsa29I ATCGAT 1 cut(s) 531
BsaHI GRCGYC 3 cut(s) 63, 273, 348
BsaJI CCNNGG 2 cut(s) 247, 443
BsaWI WCCGGW 1 cut(s) 1000
BsaXI ACNNNNNCTCC 4 cut(s) 185, 215, 669, 699
Bse3DI GCAATG 2 cut(s) 951, 1161
BseCI ATCGAT 1 cut(s) 531
BseDI CCNNGG 2 cut(s) 247, 443
BseGI GGATG 2 cut(s) 784, 1009
BseMI GCAATG 2 cut(s) 951, 1161
BseMII CTCAG 1 cut(s) 552
BseRI GAGGAG 1 cut(s) 1048
BseSI GKGCMC 2 cut(s) 77, 1127
BseX3I CGGCCG 1 cut(s) 250
BseXI GCAGC 5 cut(s) 226, 592, 931, 953, 1076
BseYI CCCAGC 2 cut(s) 39, 500
Bsh1236I CGCG 2 cut(s) 249, 255
Bsh1285I CGRYCG 1 cut(s) 253
BshFI GGCC 1 cut(s) 252
BshNI GGYRCC 2 cut(s) 62, 74
BshVI ATCGAT 1 cut(s) 531
BsiEI CGRYCG 1 cut(s) 253
BsiHKAI GWGCWC 1 cut(s) 1112
BsiSI CCGG 2 cut(s) 1001, 1127
BslFI GGGAC 1 cut(s) 582
BsmAI GTCTC 1 cut(s) 175
BsmFI GGGAC 1 cut(s) 582
BsnI GGCC 1 cut(s) 252
Bsp1286I GDGCHC 3 cut(s) 77, 1112, 1127
Bsp143I GATC 8 cut(s) 144, 175, 309, 468, 745, 958, 1043, 1143
BspANI GGCC 1 cut(s) 252
BspCNI CTCAG 1 cut(s) 551
BspDI ATCGAT 1 cut(s) 531
BspFNI CGCG 2 cut(s) 249, 255
BspHI TCATGA 1 cut(s) 628
BspLI GGNNCC 5 cut(s) 64, 76, 283, 480, 949
BspMAI CTGCAG 3 cut(s) 615, 707, 1136
BspPI GGATC 3 cut(s) 139, 953, 1138
BspT107I GGYRCC 2 cut(s) 62, 74
BsrBI CCGCTC 1 cut(s) 183
BsrDI GCAATG 2 cut(s) 951, 1161
BssECI CCNNGG 2 cut(s) 247, 443
BssMI GATC 8 cut(s) 144, 175, 309, 468, 745, 958, 1043, 1143
BssNI GRCGYC 3 cut(s) 63, 273, 348
BssT1I CCWWGG 1 cut(s) 443
Bst4CI ACNGT 1 cut(s) 565
BstACI GRCGYC 3 cut(s) 63, 273, 348
BstAPI GCANNNNNTGC 1 cut(s) 938
BstC8I GCNNGC 4 cut(s) 930, 934, 1064, 1136
BstDEI CTNAG 3 cut(s) 55, 538, 1173
BstDSI CCRYGG 1 cut(s) 247
BstF5I GGATG 2 cut(s) 784, 1009
BstFNI CGCG 2 cut(s) 249, 255
BstH2I RGCGCY 1 cut(s) 66
BstHHI GCGC 2 cut(s) 65, 1161
BstKTI GATC 8 cut(s) 147, 178, 312, 471, 748, 961, 1046, 1146
BstMAI GTCTC 1 cut(s) 175
BstMBI GATC 8 cut(s) 144, 175, 309, 468, 745, 958, 1043, 1143
BstMCI CGRYCG 1 cut(s) 253
BstMWI GCNNNNNNNGC 9 cut(s) 92, 147, 434, 580, 916, 925, 938, 1086, 1131
BstSCI CCNGG 1 cut(s) 1125
BstSFI CTRYAG 3 cut(s) 611, 703, 1132
BstSLI GKGCMC 2 cut(s) 77, 1127
BstUI CGCG 2 cut(s) 249, 255
BstV1I GCAGC 5 cut(s) 226, 592, 931, 953, 1076
BstV2I GAAGAC 2 cut(s) 204, 1045
BstX2I RGATCY 2 cut(s) 144, 1143
BstYI RGATCY 2 cut(s) 144, 1143
BstZI CGGCCG 1 cut(s) 250
Bsu15I ATCGAT 1 cut(s) 531
BsuI GTATCC 1 cut(s) 714
BsuRI GGCC 1 cut(s) 252
BsuTUI ATCGAT 1 cut(s) 531
BtgI CCRYGG 1 cut(s) 247
BtgZI GCGATG 1 cut(s) 951
BtrI CACGTC 1 cut(s) 1113
BtsCI GGATG 2 cut(s) 784, 1009
BtsI GCAGTG 1 cut(s) 1149
BtsIMutI CAGTG 2 cut(s) 694, 1149
Cac8I GCNNGC 4 cut(s) 930, 934, 1064, 1136
CciI TCATGA 1 cut(s) 628
CciNI GCGGCCGC 1 cut(s) 250
CfoI GCGC 2 cut(s) 65, 1161
Cfr42I CCGCGG 1 cut(s) 250
ClaI ATCGAT 1 cut(s) 531
CseI GACGC 4 cut(s) 244, 281, 356, 376
Csp6I GTAC 1 cut(s) 998
CviAII CATG 2 cut(s) 629, 738
CviQI GTAC 1 cut(s) 998
DdeI CTNAG 3 cut(s) 55, 538, 1173
DinI GGCGCC 1 cut(s) 64
DpnI GATC 8 cut(s) 146, 177, 311, 470, 747, 960, 1045, 1145
DpnII GATC 8 cut(s) 144, 175, 309, 468, 745, 958, 1043, 1143
EaeI YGGCCR 1 cut(s) 250
EagI CGGCCG 1 cut(s) 250
EciI GGCGGA 3 cut(s) 121, 137, 424
EclXI CGGCCG 1 cut(s) 250
Eco130I CCWWGG 1 cut(s) 443
Eco52I CGGCCG 1 cut(s) 250
EcoRI GAATTC 1 cut(s) 505
EcoT14I CCWWGG 1 cut(s) 443
EgeI GGCGCC 1 cut(s) 64
EheI GGCGCC 1 cut(s) 64
ErhI CCWWGG 1 cut(s) 443
FaeI CATG 2 cut(s) 632, 741
FalI AAGNNNNNCTT 2 cut(s) 569, 601
FaqI GGGAC 1 cut(s) 582
FatI CATG 2 cut(s) 628, 737
FauI CCCGC 1 cut(s) 1055
FblI GTMKAC 1 cut(s) 646
Fnu4HI GCNGC 8 cut(s) 93, 215, 250, 253, 581, 920, 942, 1090
FokI GGATG 2 cut(s) 791, 1016
Fsp4HI GCNGC 8 cut(s) 93, 215, 250, 253, 581, 920, 942, 1090
FspAI RTGCGCAY 1 cut(s) 1160
FspBI CTAG 2 cut(s) 23, 594
FspI TGCGCA 1 cut(s) 1160
GlaI GCGC 2 cut(s) 64, 1160
GluI GCNGC 8 cut(s) 93, 215, 250, 253, 581, 920, 942, 1090
GsaI CCCAGC 2 cut(s) 43, 504
HaeII RGCGCY 1 cut(s) 66
HaeIII GGCC 1 cut(s) 252
HapII CCGG 2 cut(s) 1001, 1127
HgaI GACGC 4 cut(s) 244, 281, 356, 376
HhaI GCGC 2 cut(s) 65, 1161
Hin1I GRCGYC 3 cut(s) 63, 273, 348
Hin1II CATG 2 cut(s) 632, 741
Hin6I GCGC 2 cut(s) 63, 1159
HinP1I GCGC 2 cut(s) 63, 1159
HindIII AAGCTT 1 cut(s) 233
HinfI GANTC 6 cut(s) 101, 200, 458, 678, 858, 1169
HpaII CCGG 2 cut(s) 1001, 1127
HphI GGTGA 2 cut(s) 506, 1107
Hpy166II GTNNAC 2 cut(s) 193, 647
Hpy188I TCNGA 7 cut(s) 175, 205, 223, 363, 511, 525, 1029
Hpy188III TCNNGA 3 cut(s) 540, 629, 797
Hpy8I GTNNAC 2 cut(s) 193, 647
Hpy99I CGWCG 6 cut(s) 10, 86, 101, 275, 281, 350
HpyAV CCTTC 6 cut(s) 97, 115, 228, 388, 909, 1060
HpyCH4III ACNGT 1 cut(s) 565
HpyCH4IV ACGT 1 cut(s) 1112
HpyCH4V TGCA 7 cut(s) 613, 705, 878, 941, 1092, 1134, 1154
HpyF10VI GCNNNNNNNGC 9 cut(s) 92, 147, 434, 580, 916, 925, 938, 1086, 1131
HpyF3I CTNAG 3 cut(s) 55, 538, 1173
HpySE526I ACGT 1 cut(s) 1112
Hsp92I GRCGYC 3 cut(s) 63, 273, 348
Hsp92II CATG 2 cut(s) 632, 741
HspAI GCGC 2 cut(s) 63, 1159
KasI GGCGCC 1 cut(s) 62
KspI CCGCGG 1 cut(s) 250
Kzo9I GATC 8 cut(s) 144, 175, 309, 468, 745, 958, 1043, 1143
LmnI GCTCC 1 cut(s) 188
Lsp1109I GCAGC 5 cut(s) 226, 592, 931, 953, 1076
LweI GCATC 3 cut(s) 214, 928, 1075
MaeI CTAG 2 cut(s) 23, 594
MaeII ACGT 1 cut(s) 1112
MaeIII GTNAC 2 cut(s) 559, 1113
MalI GATC 8 cut(s) 146, 177, 311, 470, 747, 960, 1045, 1145
MbiI CCGCTC 1 cut(s) 183
MboI GATC 8 cut(s) 144, 175, 309, 468, 745, 958, 1043, 1143
MboII GAAGA 6 cut(s) 209, 659, 668, 740, 989, 1050
MflI RGATCY 2 cut(s) 144, 1143
MhlI GDGCHC 3 cut(s) 77, 1112, 1127
MluCI AATT 6 cut(s) 431, 505, 618, 883, 893, 966
Mly113I GGCGCC 1 cut(s) 63
MlyI GAGTC 3 cut(s) 194, 687, 1163
MmeI TCCRAC 2 cut(s) 228, 835
MroXI GAANNNNTTC 1 cut(s) 663
MseI TTAA 1 cut(s) 588
MslI CAYNNNNRTG 2 cut(s) 1120, 1154
MspA1I CMGCKG 2 cut(s) 249, 944
MspI CCGG 2 cut(s) 1001, 1127
MspR9I CCNGG 1 cut(s) 1127
MvnI CGCG 2 cut(s) 249, 255
MwoI GCNNNNNNNGC 9 cut(s) 92, 147, 434, 580, 916, 925, 938, 1086, 1131
NarI GGCGCC 1 cut(s) 63
NciI CCSGG 1 cut(s) 1127
NdeII GATC 8 cut(s) 144, 175, 309, 468, 745, 958, 1043, 1143
NlaIII CATG 2 cut(s) 632, 741
NlaIV GGNNCC 5 cut(s) 64, 76, 283, 480, 949
NmuCI GTSAC 2 cut(s) 559, 1113
NotI GCGGCCGC 1 cut(s) 250
NsbI TGCGCA 1 cut(s) 1160
OliI CACNNNNGTG 1 cut(s) 1120
PagI TCATGA 1 cut(s) 628
PcsI WCGNNNNNNNCGW 3 cut(s) 96, 201, 354
PdmI GAANNNNTTC 1 cut(s) 663
PfeI GAWTC 3 cut(s) 101, 458, 858
PflFI GACNNNGTC 1 cut(s) 350
PkrI GCNGC 8 cut(s) 94, 216, 251, 254, 582, 921, 943, 1091
PleI GAGTC 3 cut(s) 194, 686, 1163
PluTI GGCGCC 1 cut(s) 66
PpsI GAGTC 3 cut(s) 194, 686, 1163
PspFI CCCAGC 2 cut(s) 39, 500
PspN4I GGNNCC 5 cut(s) 64, 76, 283, 480, 949
PstI CTGCAG 3 cut(s) 615, 707, 1136
PsuI RGATCY 2 cut(s) 144, 1143
PsyI GACNNNGTC 1 cut(s) 350
PvuII CAGCTG 1 cut(s) 944
RsaI GTAC 1 cut(s) 999
RsaNI GTAC 1 cut(s) 998
RseI CAYNNNNRTG 2 cut(s) 1120, 1154
SacII CCGCGG 1 cut(s) 250
SaqAI TTAA 1 cut(s) 588
SatI GCNGC 8 cut(s) 93, 215, 250, 253, 581, 920, 942, 1090
Sau3AI GATC 8 cut(s) 144, 175, 309, 468, 745, 958, 1043, 1143
SchI GAGTC 3 cut(s) 194, 687, 1163
ScrFI CCNGG 1 cut(s) 1127
SduI GDGCHC 3 cut(s) 77, 1112, 1127
SfaNI GCATC 3 cut(s) 214, 928, 1075
SfcI CTRYAG 3 cut(s) 611, 703, 1132
SfoI GGCGCC 1 cut(s) 64
Sfr303I CCGCGG 1 cut(s) 250
SgrBI CCGCGG 1 cut(s) 250
SmiMI CAYNNNNRTG 2 cut(s) 1120, 1154
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
Sse9I AATT 6 cut(s) 431, 505, 618, 883, 893, 966
SspDI GGCGCC 1 cut(s) 62
SspMI CTAG 2 cut(s) 23, 594
StyD4I CCNGG 1 cut(s) 1125
StyI CCWWGG 1 cut(s) 443
TaaI ACNGT 1 cut(s) 565
TaiI ACGT 1 cut(s) 1115
TaqI TCGA 6 cut(s) 8, 99, 345, 531, 681, 798
TaqII GACCGA 1 cut(s) 632
TasI AATT 6 cut(s) 431, 505, 618, 883, 893, 966
TauI GCSGC 3 cut(s) 95, 252, 255
TfiI GAWTC 3 cut(s) 101, 458, 858
Tru1I TTAA 1 cut(s) 588
Tru9I TTAA 1 cut(s) 588
TscAI CASTG 2 cut(s) 694, 1156
TseFI GTSAC 2 cut(s) 559, 1113
TseI GCWGC 5 cut(s) 214, 580, 919, 941, 1089
Tsp45I GTSAC 2 cut(s) 559, 1113
TspDTI ATGAA 2 cut(s) 669, 1042
TspGWI ACGGA 3 cut(s) 151, 294, 1047
TspRI CASTG 2 cut(s) 694, 1156
Tth111I GACNNNGTC 1 cut(s) 350
XapI RAATTY 3 cut(s) 505, 618, 893
XmiI GTMKAC 1 cut(s) 646
XmnI GAANNNNTTC 1 cut(s) 663
XspI CTAG 2 cut(s) 23, 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.