RchiOBHm_Chr2g0166771

mitochondrial saccharopine dehydrogenase-like oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002135
Physical Location & Seq
Reverse (-)
81410755 .. 81412899
2145 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1356 bp
ATGTCAGACCTCCCCAACCCTCTCTACGATTTAATCATCCTCGGCGCCTCCGGCTTCACCGGAAAGCACGTCGTGAAAGAAAGCCTCAAGTTCCTCAACACCCCATCTTCACCTCTCAAGTCTCTAGCTTTAGCCGGCCGCAACCCCGCAAAGCTCTCCCAAACTCTCAACTGGGCCACCGGCCCAAACCCACCTCCCTCCATTCCCATCCTCACCGCCGACACCACCGACCCTCCGTCCCTCCGCCGCCTCTGCTCCCAGACCCGCCTCATCCTCAACTGCGTGGGCCCCTTCCGCCTCTACGGCGAACCCGTCGTTGCCGCGTGCGTTGAGACCGGCTGTGATTATCTCGACATATGCGGAGAGCCGGAGTTCATGGAGAGGATGGAGGCCAGTTATTTCGATAAGGCGGCGGAGAAGGGCTCGTTGGTCATTTCGGCTTGTGGGTTCGACTCTGTTCCGGCGGAGTTTGGGTTGATGTTTAACTCAAGGCACTGGGTGGGGGCGGCAGTGCCGAGTAGAGTGGAGGCTTATGTGAGCTTGGAGTCCGAGAAGAGAATTGTTGGCAACTTCGGGACGTTTGAGTCGGCGGTGTTGGGTGTGGCTAATGCGGATAAGTTGATGAAGCTCCGGCGGTCCAGACCTAAAAAGCCGAGGCCGGAGATTCCTGCTTCTCCTCCTTCCAAAGGACCAACTATTGAACACCAGAAAAAGATCGGCCTTTGGGCTGTGAAGCTACCTTCAGCAGATTCTACCGTTGTACGAAGAACACTTGGAGCTCTGACCGAAAATCCCGGTGGTCTTCCAGGGAGGAATGAGAGTGATGAACACATTAAAAAAAGAGAAGCCTTCTGGTCAACAGTGAAGCCAGCTCATTTCGGGATGAAGATAGGCACCAAGTCTCTGTTGGGCATTTTTCGGATCATGACAGTTGGAATTTTTATTGGGCTCTTGGGTCAGTTTTCCTTTGGGAGGTGGCTTCTCTTAAAGTATCCCCCATTTTTCAGTGTTGGATTGTTCAGGAAGCAGGGTCCCTCCGATAAGGAGGTGAGAAGTGCATCTTTTAAGATGTGGTTTGTTGGATGTGGTTTCAGCGACAGGAGTCTTGCTTCTCAGGGAAACACAAAACCTGATATGGAAATAGTAACAAGAGTTATGGGACCTGAAGTTGGCTACATAACAACCCCGATAATTCTACTTCAGTGTGCACTTATTGTTCTGAGCCAACGCGACAATCTTCCAAAGGGAGGAGTTCTCCCACCTGGGATTGTATTTGGCCCAACTGATCTCCAAGAAAAGCTACAAGAAAATGGAGTTACTTTTGATGTTATTTCAAAGAATGTTATTTCTCATTAA

Protein Analysis

451

Amino Acids

49.14

Weight (kDa)

9.28

Isoelectric Point (pI)

44.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sacchrp_dh_NADP PF03435 12 - 144 4.4e-11 Saccharopine dehydrogenase NADP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 583
AccB1I GGYRCC 2 cut(s) 44, 893
AccII CGCG 2 cut(s) 323, 1230
AclWI GGATC 1 cut(s) 929
AcoI YGGCCR 1 cut(s) 136
AcsI RAATTY 1 cut(s) 936
AcuI CTGAAG 3 cut(s) 726, 1184, 1185
AcyI GRCGYC 1 cut(s) 45
AdeI CACNNNGTG 2 cut(s) 73, 499
AfaI GTAC 1 cut(s) 762
AfiI CCNNNNNNNGG 4 cut(s) 686, 972, 1169, 1247
AgsI TTSAA 2 cut(s) 701, 1335
AhdI GACNNNNNGTC 1 cut(s) 235
AjiI CACGTC 1 cut(s) 70
AjnI CCWGG 2 cut(s) 805, 1261
AjuI GAANNNNNNNTTGG 2 cut(s) 410, 442
AluBI AGCT 8 cut(s) 128, 154, 540, 628, 736, 779, 872, 1300
AluI AGCT 8 cut(s) 128, 154, 540, 628, 736, 779, 872, 1300
Alw21I GWGCWC 2 cut(s) 781, 1210
Alw26I GTCTC 3 cut(s) 126, 326, 906
Alw44I GTGCAC 1 cut(s) 1206
AlwI GGATC 1 cut(s) 929
AoxI GGCC 8 cut(s) 136, 174, 181, 286, 390, 656, 718, 1276
ApaI GGGCCC 1 cut(s) 290
ApaLI GTGCAC 1 cut(s) 1206
ApoI RAATTY 1 cut(s) 936
AspLEI GCGC 1 cut(s) 47
AspS9I GGNCC 9 cut(s) 174, 182, 286, 287, 636, 689, 1031, 1160, 1277
AsuC2I CCSGG 1 cut(s) 795
AsuHPI GGTGA 4 cut(s) 49, 102, 205, 1060
AvaII GGWCC 4 cut(s) 636, 689, 1031, 1160
BaeGI GKGCMC 2 cut(s) 290, 1210
BanI GGYRCC 2 cut(s) 44, 893
BanII GRGCYC 4 cut(s) 290, 425, 781, 951
BbsI GAAGAC 1 cut(s) 794
Bbv12I GWGCWC 2 cut(s) 781, 1210
BccI CCATC 3 cut(s) 112, 215, 379
BceAI ACGGC 1 cut(s) 319
BciT130I CCWGG 2 cut(s) 807, 1263
BciVI GTATCC 1 cut(s) 1002
BcnI CCSGG 1 cut(s) 795
BcoDI GTCTC 3 cut(s) 126, 326, 906
BfaI CTAG 1 cut(s) 125
BfoI RGCGCY 1 cut(s) 48
BfuI GTATCC 1 cut(s) 1002
BglI GCCNNNNNGGC 1 cut(s) 303
BisI GCNGC 5 cut(s) 139, 247, 321, 411, 507
BlsI GCNGC 5 cut(s) 140, 248, 322, 412, 508
Bme1390I CCNGG 3 cut(s) 795, 807, 1263
Bme18I GGWCC 4 cut(s) 636, 689, 1031, 1160
BmeRI GACNNNNNGTC 1 cut(s) 235
BmgBI CACGTC 1 cut(s) 70
BmgT120I GGNCC 9 cut(s) 174, 182, 286, 287, 636, 689, 1031, 1160, 1277
BmiI GGNNCC 7 cut(s) 46, 288, 289, 895, 1032, 1033, 1161
BmrFI CCNGG 3 cut(s) 795, 807, 1263
BmrI ACTGGG 2 cut(s) 181, 505
BmsI GCATC 1 cut(s) 1067
BmuI ACTGGG 2 cut(s) 181, 505
BoxI GACNNNNGTC 1 cut(s) 1101
BpiI GAAGAC 1 cut(s) 794
BplI GAGNNNNNCTC 4 cut(s) 407, 439, 1239, 1271
BpuEI CTTGAG 3 cut(s) 71, 101, 472
BpuMI CCSGG 1 cut(s) 795
BsaHI GRCGYC 1 cut(s) 45
BsaI GGTCTC 1 cut(s) 326
BsaJI CCNNGG 4 cut(s) 40, 653, 806, 1262
BsaWI WCCGGW 1 cut(s) 59
BsaXI ACNNNNNCTCC 4 cut(s) 217, 247, 458, 488
Bsc4I CCNNNNNNNGG 4 cut(s) 686, 972, 1169, 1247
Bse118I RCCGGY 3 cut(s) 134, 179, 335
Bse1I ACTGG 3 cut(s) 176, 393, 500
BseBI CCWGG 2 cut(s) 807, 1263
BseDI CCNNGG 4 cut(s) 40, 653, 806, 1262
BseGI GGATG 6 cut(s) 36, 207, 270, 390, 888, 1088
BseLI CCNNNNNNNGG 4 cut(s) 686, 972, 1169, 1247
BseMII CTCAG 2 cut(s) 1127, 1211
BseNI ACTGG 3 cut(s) 176, 393, 500
BseRI GAGGAG 2 cut(s) 666, 1263
BseSI GKGCMC 2 cut(s) 290, 1210
BseX3I CGGCCG 1 cut(s) 136
Bsh1236I CGCG 2 cut(s) 323, 1230
Bsh1285I CGRYCG 1 cut(s) 139
BshFI GGCC 8 cut(s) 138, 176, 183, 288, 392, 658, 720, 1278
BshNI GGYRCC 2 cut(s) 44, 893
BsiEI CGRYCG 1 cut(s) 139
BsiHKAI GWGCWC 2 cut(s) 781, 1210
BslFI GGGAC 4 cut(s) 223, 589, 1017, 1173
BslI CCNNNNNNNGG 4 cut(s) 686, 972, 1169, 1247
BsmAI GTCTC 3 cut(s) 126, 326, 906
BsmFI GGGAC 4 cut(s) 223, 589, 1017, 1173
BsnI GGCC 8 cut(s) 138, 176, 183, 288, 392, 658, 720, 1278
Bso31I GGTCTC 1 cut(s) 326
Bsp120I GGGCCC 1 cut(s) 286
Bsp1286I GDGCHC 5 cut(s) 290, 425, 781, 951, 1210
Bsp143I GATC 3 cut(s) 714, 921, 1285
BspANI GGCC 8 cut(s) 138, 176, 183, 288, 392, 658, 720, 1278
BspCNI CTCAG 2 cut(s) 1126, 1212
BspFNI CGCG 2 cut(s) 323, 1230
BspHI TCATGA 1 cut(s) 924
BspLI GGNNCC 7 cut(s) 46, 288, 289, 895, 1032, 1033, 1161
BspPI GGATC 1 cut(s) 929
BspT107I GGYRCC 2 cut(s) 44, 893
BspTNI GGTCTC 1 cut(s) 326
BsrFI RCCGGY 3 cut(s) 134, 179, 335
BsrI ACTGG 3 cut(s) 176, 393, 500
BssAI RCCGGY 3 cut(s) 134, 179, 335
BssECI CCNNGG 4 cut(s) 40, 653, 806, 1262
BssMI GATC 3 cut(s) 714, 921, 1285
BssNI GRCGYC 1 cut(s) 45
Bst2UI CCWGG 2 cut(s) 807, 1263
Bst4CI ACNGT 3 cut(s) 757, 862, 931
Bst6I CTCTTC 1 cut(s) 548
BstACI GRCGYC 1 cut(s) 45
BstC8I GCNNGC 3 cut(s) 136, 325, 870
BstDEI CTNAG 2 cut(s) 1113, 1220
BstENI CCTNNNNNAGG 2 cut(s) 684, 970
BstF5I GGATG 6 cut(s) 36, 207, 270, 390, 888, 1088
BstFNI CGCG 2 cut(s) 323, 1230
BstH2I RGCGCY 1 cut(s) 48
BstHHI GCGC 1 cut(s) 47
BstKTI GATC 3 cut(s) 717, 924, 1288
BstMAI GTCTC 3 cut(s) 126, 326, 906
BstMBI GATC 3 cut(s) 714, 921, 1285
BstMCI CGRYCG 1 cut(s) 139
BstMWI GCNNNNNNNGC 4 cut(s) 51, 252, 294, 303
BstNI CCWGG 2 cut(s) 807, 1263
BstPAI GACNNNNGTC 1 cut(s) 1101
BstSCI CCNGG 3 cut(s) 793, 805, 1261
BstSLI GKGCMC 2 cut(s) 290, 1210
BstUI CGCG 2 cut(s) 323, 1230
BstV2I GAAGAC 1 cut(s) 794
BstZI CGGCCG 1 cut(s) 136
BsuI GTATCC 1 cut(s) 1002
BsuRI GGCC 8 cut(s) 138, 176, 183, 288, 392, 658, 720, 1278
BtrI CACGTC 1 cut(s) 70
BtsCI GGATG 6 cut(s) 36, 207, 270, 390, 888, 1088
BtsI GCAGTG 1 cut(s) 516
BtsIMutI CAGTG 5 cut(s) 493, 516, 867, 1012, 1208
Cac8I GCNNGC 3 cut(s) 136, 325, 870
CciI TCATGA 1 cut(s) 924
CfoI GCGC 1 cut(s) 47
Cfr10I RCCGGY 3 cut(s) 134, 179, 335
Cfr13I GGNCC 9 cut(s) 174, 182, 286, 287, 636, 689, 1031, 1160, 1277
Csp6I GTAC 1 cut(s) 761
CviAII CATG 2 cut(s) 376, 925
CviQI GTAC 1 cut(s) 761
DdeI CTNAG 2 cut(s) 1113, 1220
DinI GGCGCC 1 cut(s) 46
DpnI GATC 3 cut(s) 716, 923, 1287
DpnII GATC 3 cut(s) 714, 921, 1285
DraIII CACNNNGTG 2 cut(s) 73, 499
DrdI GACNNNNNNGTC 1 cut(s) 583
DriI GACNNNNNGTC 1 cut(s) 235
DseDI GACNNNNNNGTC 1 cut(s) 583
EaeI YGGCCR 1 cut(s) 136
EagI CGGCCG 1 cut(s) 136
Eam1104I CTCTTC 1 cut(s) 548
Eam1105I GACNNNNNGTC 1 cut(s) 235
EarI CTCTTC 1 cut(s) 548
EciI GGCGGA 4 cut(s) 233, 284, 428, 479
Ecl136II GAGCTC 1 cut(s) 779
EclXI CGGCCG 1 cut(s) 136
Eco24I GRGCYC 4 cut(s) 290, 425, 781, 951
Eco31I GGTCTC 1 cut(s) 326
Eco47I GGWCC 4 cut(s) 636, 689, 1031, 1160
Eco52I CGGCCG 1 cut(s) 136
Eco53kI GAGCTC 1 cut(s) 779
Eco57I CTGAAG 3 cut(s) 726, 1184, 1185
EcoICRI GAGCTC 1 cut(s) 779
EcoNI CCTNNNNNAGG 2 cut(s) 684, 970
EcoO109I RGGNCCY 3 cut(s) 287, 1031, 1160
EcoRII CCWGG 2 cut(s) 805, 1261
EcoT38I GRGCYC 4 cut(s) 290, 425, 781, 951
EgeI GGCGCC 1 cut(s) 46
EheI GGCGCC 1 cut(s) 46
FaeI CATG 2 cut(s) 379, 928
FaiI YATR 8 cut(s) 356, 358, 377, 534, 926, 1136, 1157, 1178
FalI AAGNNNNNCTT 2 cut(s) 1045, 1077
FaqI GGGAC 4 cut(s) 223, 589, 1017, 1173
FatI CATG 2 cut(s) 375, 924
FauI CCCGC 2 cut(s) 154, 272
FauNDI CATATG 1 cut(s) 356
Fnu4HI GCNGC 5 cut(s) 139, 247, 321, 411, 507
FokI GGATG 6 cut(s) 23, 194, 257, 397, 895, 1095
FriOI GRGCYC 4 cut(s) 290, 425, 781, 951
Fsp4HI GCNGC 5 cut(s) 139, 247, 321, 411, 507
FspBI CTAG 1 cut(s) 125
GlaI GCGC 1 cut(s) 46
GluI GCNGC 5 cut(s) 139, 247, 321, 411, 507
HaeII RGCGCY 1 cut(s) 48
HaeIII GGCC 8 cut(s) 138, 176, 183, 288, 392, 658, 720, 1278
HhaI GCGC 1 cut(s) 47
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 2 cut(s) 379, 928
Hin6I GCGC 1 cut(s) 45
HinP1I GCGC 1 cut(s) 45
HincII GTYRAC 1 cut(s) 858
HindII GTYRAC 1 cut(s) 858
HinfI GANTC 6 cut(s) 452, 545, 584, 664, 749, 1102
HphI GGTGA 4 cut(s) 49, 102, 205, 1060
Hpy166II GTNNAC 2 cut(s) 858, 1208
Hpy188I TCNGA 6 cut(s) 7, 550, 783, 921, 1039, 1221
Hpy188III TCNNGA 7 cut(s) 73, 350, 574, 639, 880, 925, 1021
Hpy8I GTNNAC 2 cut(s) 858, 1208
Hpy99I CGWCG 2 cut(s) 74, 317
HpyAV CCTTC 5 cut(s) 301, 412, 690, 750, 859
HpyCH4III ACNGT 3 cut(s) 757, 862, 931
HpyCH4IV ACGT 2 cut(s) 69, 578
HpyCH4V TGCA 2 cut(s) 1058, 1208
HpyF10VI GCNNNNNNNGC 4 cut(s) 51, 252, 294, 303
HpyF3I CTNAG 2 cut(s) 1113, 1220
HpySE526I ACGT 2 cut(s) 69, 578
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 2 cut(s) 379, 928
HspAI GCGC 1 cut(s) 45
KasI GGCGCC 1 cut(s) 44
KflI GGGWCCC 1 cut(s) 1031
KroI GCCGGC 1 cut(s) 134
KroNI GCCGGC 1 cut(s) 136
Kzo9I GATC 3 cut(s) 714, 921, 1285
LmnI GCTCC 3 cut(s) 260, 633, 776
LweI GCATC 1 cut(s) 1067
MaeI CTAG 1 cut(s) 125
MaeII ACGT 2 cut(s) 69, 578
MaeIII GTNAC 2 cut(s) 1144, 1315
MalI GATC 3 cut(s) 716, 923, 1287
MboI GATC 3 cut(s) 714, 921, 1285
MboII GAAGA 6 cut(s) 99, 565, 777, 794, 898, 1229
MhlI GDGCHC 5 cut(s) 290, 425, 781, 951, 1210
MluCI AATT 3 cut(s) 558, 936, 1191
Mly113I GGCGCC 1 cut(s) 45
MlyI GAGTC 4 cut(s) 446, 554, 593, 1111
MmeI TCCRAC 3 cut(s) 913, 991, 1060
MroNI GCCGGC 1 cut(s) 134
MseI TTAA 6 cut(s) 32, 483, 834, 986, 1065, 1354
MspR9I CCNGG 3 cut(s) 795, 807, 1263
MvaI CCWGG 2 cut(s) 807, 1263
MvnI CGCG 2 cut(s) 323, 1230
MwoI GCNNNNNNNGC 4 cut(s) 51, 252, 294, 303
NaeI GCCGGC 1 cut(s) 136
NarI GGCGCC 1 cut(s) 45
NciI CCSGG 1 cut(s) 795
NdeI CATATG 1 cut(s) 356
NdeII GATC 3 cut(s) 714, 921, 1285
NgoMIV GCCGGC 1 cut(s) 134
NlaIII CATG 2 cut(s) 379, 928
NlaIV GGNNCC 7 cut(s) 46, 288, 289, 895, 1032, 1033, 1161
NmeAIII GCCGAG 3 cut(s) 21, 540, 678
PagI TCATGA 1 cut(s) 924
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PdiI GCCGGC 1 cut(s) 136
PfeI GAWTC 2 cut(s) 664, 749
PkrI GCNGC 5 cut(s) 140, 248, 322, 412, 508
PleI GAGTC 4 cut(s) 446, 553, 592, 1110
PluTI GGCGCC 1 cut(s) 48
PpsI GAGTC 4 cut(s) 446, 553, 592, 1110
PpuMI RGGWCCY 2 cut(s) 1031, 1160
PshAI GACNNNNGTC 1 cut(s) 1101
Psp124BI GAGCTC 1 cut(s) 781
Psp5II RGGWCCY 2 cut(s) 1031, 1160
Psp6I CCWGG 2 cut(s) 805, 1261
PspGI CCWGG 2 cut(s) 805, 1261
PspN4I GGNNCC 7 cut(s) 46, 288, 289, 895, 1032, 1033, 1161
PspOMI GGGCCC 1 cut(s) 286
PspPI GGNCC 9 cut(s) 174, 182, 286, 287, 636, 689, 1031, 1160, 1277
PspPPI RGGWCCY 2 cut(s) 1031, 1160
RsaI GTAC 1 cut(s) 762
RsaNI GTAC 1 cut(s) 761
SacI GAGCTC 1 cut(s) 781
SaqAI TTAA 6 cut(s) 32, 483, 834, 986, 1065, 1354
SatI GCNGC 5 cut(s) 139, 247, 321, 411, 507
Sau3AI GATC 3 cut(s) 714, 921, 1285
Sau96I GGNCC 9 cut(s) 174, 182, 286, 287, 636, 689, 1031, 1160, 1277
SchI GAGTC 4 cut(s) 446, 554, 593, 1111
ScrFI CCNGG 3 cut(s) 795, 807, 1263
SduI GDGCHC 5 cut(s) 290, 425, 781, 951, 1210
SfaNI GCATC 1 cut(s) 1067
SfoI GGCGCC 1 cut(s) 46
SinI GGWCC 4 cut(s) 636, 689, 1031, 1160
SmlI CTYRAG 3 cut(s) 86, 116, 487
SmoI CTYRAG 3 cut(s) 86, 116, 487
Sse9I AATT 3 cut(s) 558, 936, 1191
SspDI GGCGCC 1 cut(s) 44
SspMI CTAG 1 cut(s) 125
SstI GAGCTC 1 cut(s) 781
StyD4I CCNGG 3 cut(s) 793, 805, 1261
TaaI ACNGT 3 cut(s) 757, 862, 931
TaiI ACGT 2 cut(s) 72, 581
TaqI TCGA 3 cut(s) 351, 402, 450
TaqII GACCGA 1 cut(s) 800
TasI AATT 3 cut(s) 558, 936, 1191
TauI GCSGC 5 cut(s) 141, 249, 323, 413, 509
TfiI GAWTC 2 cut(s) 664, 749
Tru1I TTAA 6 cut(s) 32, 483, 834, 986, 1065, 1354
Tru9I TTAA 6 cut(s) 32, 483, 834, 986, 1065, 1354
TscAI CASTG 5 cut(s) 500, 516, 867, 1012, 1208
TspDTI ATGAA 4 cut(s) 364, 638, 840, 899
TspGWI ACGGA 1 cut(s) 225
TspRI CASTG 5 cut(s) 500, 516, 867, 1012, 1208
VneI GTGCAC 1 cut(s) 1206
VpaK11BI GGWCC 4 cut(s) 636, 689, 1031, 1160
XagI CCTNNNNNAGG 2 cut(s) 684, 970
XapI RAATTY 1 cut(s) 936
XcmI CCANNNNNNNNNTGG 1 cut(s) 904
XspI CTAG 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.