RchiOBHm_Chr1g0321691

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0006394
Physical Location & Seq
Reverse (-)
9149134 .. 9149836
703 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 516 bp
ATGAGCGACATGTGTCCTCATCTATTAGCTGGTCCAGGTATAAAGCCTCATCTTGTATCCTGTAACCATTATGGATCAATATATCATAATTTCTCTTTTGCATTTTTTTCCTTCTGCATTTCTTTTCTTCGTTGGGGTCTTACCCTAACAACTGGTATCAAGAGCCAGACGACCATGGTTAAGCTTAAGGGGGTGGGGAAAACTGCGGATCCGCCTCGCAACCCGAAGGCCCCTCTTGTATCGGATGAGCTTTCTCACCTCGAATACGAGTTCCAGATCCACAGAGATGAGACCAGTGCCCGCTTGGAGGAGCTACAAGCTTCGATCCAGGACTCTCTAGCTACTTCCTTGGCTCAATTTAAGTTGGCGATGATGGAGGAGCTCACACATCAGCTCACGTCTTCTCTTACCACAGTTGTCAAAGATGGTTTGCACACTTCTCAGTCCTTAGTGGTGAGCCAGGGGAGCACCGCAGTTCCTCAACCTGATGGAACTGTTCAATTTGGATCTATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag gag-asp_proteas rve

Protein Analysis

171

Amino Acids

18.71

Weight (kDa)

6.21

Isoelectric Point (pI)

33.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 206, 212, 301, 471
AclWI GGATC 6 cut(s) 82, 203, 216, 271, 319, 514
AfiI CCNNNNNNNGG 1 cut(s) 307
AflII CTTAAG 1 cut(s) 185
AflIII ACRYGT 1 cut(s) 9
AgsI TTSAA 1 cut(s) 500
AjiI CACGTC 1 cut(s) 399
AjnI CCWGG 3 cut(s) 34, 327, 459
AluBI AGCT 8 cut(s) 29, 184, 250, 313, 320, 341, 382, 394
AluI AGCT 8 cut(s) 29, 184, 250, 313, 320, 341, 382, 394
Alw21I GWGCWC 2 cut(s) 384, 470
Alw26I GTCTC 1 cut(s) 284
AlwI GGATC 6 cut(s) 82, 203, 216, 271, 319, 514
AoxI GGCC 1 cut(s) 228
AspS9I GGNCC 2 cut(s) 32, 229
AsuHPI GGTGA 2 cut(s) 248, 466
AvaII GGWCC 1 cut(s) 32
BaeGI GKGCMC 1 cut(s) 301
BamHI GGATCC 1 cut(s) 208
BanII GRGCYC 1 cut(s) 384
BbsI GAAGAC 1 cut(s) 393
Bbv12I GWGCWC 2 cut(s) 384, 470
BccI CCATC 3 cut(s) 367, 419, 482
BciT130I CCWGG 3 cut(s) 36, 329, 461
BciVI GTATCC 1 cut(s) 67
BcoDI GTCTC 1 cut(s) 284
BfaI CTAG 1 cut(s) 338
BfrI CTTAAG 1 cut(s) 185
BfuI GTATCC 1 cut(s) 67
Bme1390I CCNGG 3 cut(s) 36, 329, 461
Bme18I GGWCC 1 cut(s) 32
BmgBI CACGTC 1 cut(s) 399
BmgT120I GGNCC 2 cut(s) 32, 229
BmiI GGNNCC 2 cut(s) 210, 231
BmrFI CCNGG 3 cut(s) 36, 329, 461
BoxI GACNNNNGTC 1 cut(s) 12
BpiI GAAGAC 1 cut(s) 393
BsaI GGTCTC 1 cut(s) 284
BsaJI CCNNGG 3 cut(s) 174, 348, 460
Bsc4I CCNNNNNNNGG 1 cut(s) 307
Bse1I ACTGG 2 cut(s) 157, 294
BseBI CCWGG 3 cut(s) 36, 329, 461
BseDI CCNNGG 3 cut(s) 174, 348, 460
BseGI GGATG 1 cut(s) 250
BseLI CCNNNNNNNGG 1 cut(s) 307
BseMII CTCAG 1 cut(s) 455
BseNI ACTGG 2 cut(s) 157, 294
BseRI GAGGAG 2 cut(s) 323, 392
BseSI GKGCMC 1 cut(s) 301
BshFI GGCC 1 cut(s) 230
BsiHKAI GWGCWC 2 cut(s) 384, 470
BslI CCNNNNNNNGG 1 cut(s) 307
BsmAI GTCTC 1 cut(s) 284
BsnI GGCC 1 cut(s) 230
Bso31I GGTCTC 1 cut(s) 284
Bsp1286I GDGCHC 3 cut(s) 301, 384, 470
Bsp143I GATC 5 cut(s) 74, 208, 276, 324, 506
Bsp19I CCATGG 1 cut(s) 174
BspACI CCGC 4 cut(s) 206, 212, 301, 471
BspANI GGCC 1 cut(s) 230
BspCNI CTCAG 1 cut(s) 454
BspLI GGNNCC 2 cut(s) 210, 231
BspPI GGATC 6 cut(s) 82, 203, 216, 271, 319, 514
BspTI CTTAAG 1 cut(s) 185
BspTNI GGTCTC 1 cut(s) 284
BsrI ACTGG 2 cut(s) 157, 294
BssECI CCNNGG 3 cut(s) 174, 348, 460
BssMI GATC 5 cut(s) 74, 208, 276, 324, 506
BssT1I CCWWGG 2 cut(s) 174, 348
Bst2UI CCWGG 3 cut(s) 36, 329, 461
Bst4CI ACNGT 2 cut(s) 415, 496
BstAFI CTTAAG 1 cut(s) 185
BstC8I GCNNGC 1 cut(s) 301
BstDEI CTNAG 2 cut(s) 441, 448
BstDSI CCRYGG 1 cut(s) 174
BstF5I GGATG 1 cut(s) 250
BstKTI GATC 5 cut(s) 77, 211, 279, 327, 509
BstMAI GTCTC 1 cut(s) 284
BstMBI GATC 5 cut(s) 74, 208, 276, 324, 506
BstMWI GCNNNNNNNGC 1 cut(s) 465
BstNI CCWGG 3 cut(s) 36, 329, 461
BstNSI RCATGY 1 cut(s) 13
BstPAI GACNNNNGTC 1 cut(s) 12
BstSCI CCNGG 3 cut(s) 34, 327, 459
BstSLI GKGCMC 1 cut(s) 301
BstV2I GAAGAC 1 cut(s) 393
BstX2I RGATCY 3 cut(s) 208, 276, 506
BstYI RGATCY 3 cut(s) 208, 276, 506
BsuI GTATCC 1 cut(s) 67
BsuRI GGCC 1 cut(s) 230
BtgI CCRYGG 1 cut(s) 174
BtgZI GCGATG 1 cut(s) 383
BtrI CACGTC 1 cut(s) 399
BtsCI GGATG 1 cut(s) 250
BtsIMutI CAGTG 1 cut(s) 301
Cac8I GCNNGC 1 cut(s) 301
Cfr13I GGNCC 2 cut(s) 32, 229
CviAII CATG 2 cut(s) 10, 175
DdeI CTNAG 2 cut(s) 441, 448
DpnI GATC 5 cut(s) 76, 210, 278, 326, 508
DpnII GATC 5 cut(s) 74, 208, 276, 324, 506
EciI GGCGGA 1 cut(s) 201
Ecl136II GAGCTC 1 cut(s) 382
Eco130I CCWWGG 2 cut(s) 174, 348
Eco24I GRGCYC 1 cut(s) 384
Eco31I GGTCTC 1 cut(s) 284
Eco47I GGWCC 1 cut(s) 32
Eco53kI GAGCTC 1 cut(s) 382
EcoICRI GAGCTC 1 cut(s) 382
EcoO109I RGGNCCY 1 cut(s) 229
EcoRII CCWGG 3 cut(s) 34, 327, 459
EcoT14I CCWWGG 2 cut(s) 174, 348
EcoT38I GRGCYC 1 cut(s) 384
ErhI CCWWGG 2 cut(s) 174, 348
FaeI CATG 2 cut(s) 13, 178
FaiI YATR 6 cut(s) 11, 41, 72, 82, 87, 176
FatI CATG 2 cut(s) 9, 174
FauI CCCGC 1 cut(s) 308
FokI GGATG 1 cut(s) 257
FriOI GRGCYC 1 cut(s) 384
FspBI CTAG 1 cut(s) 338
HaeIII GGCC 1 cut(s) 230
Hin1II CATG 2 cut(s) 13, 178
HindIII AAGCTT 2 cut(s) 182, 318
HinfI GANTC 1 cut(s) 332
HphI GGTGA 2 cut(s) 248, 466
Hpy188I TCNGA 1 cut(s) 244
Hpy188III TCNNGA 2 cut(s) 160, 274
HpyAV CCTTC 2 cut(s) 121, 220
HpyCH4III ACNGT 2 cut(s) 415, 496
HpyCH4IV ACGT 1 cut(s) 398
HpyCH4V TGCA 3 cut(s) 101, 117, 433
HpyF10VI GCNNNNNNNGC 1 cut(s) 465
HpyF3I CTNAG 2 cut(s) 441, 448
HpySE526I ACGT 1 cut(s) 398
Hsp92II CATG 2 cut(s) 13, 178
Kzo9I GATC 5 cut(s) 74, 208, 276, 324, 506
LmnI GCTCC 3 cut(s) 310, 379, 465
MaeI CTAG 1 cut(s) 338
MaeII ACGT 1 cut(s) 398
MaeIII GTNAC 1 cut(s) 62
MalI GATC 5 cut(s) 76, 210, 278, 326, 508
MboI GATC 5 cut(s) 74, 208, 276, 324, 506
MboII GAAGA 2 cut(s) 119, 393
MflI RGATCY 3 cut(s) 208, 276, 506
MhlI GDGCHC 3 cut(s) 301, 384, 470
MluCI AATT 3 cut(s) 88, 356, 500
MlyI GAGTC 1 cut(s) 326
MnlI CCTC 8 cut(s) 27, 57, 225, 243, 269, 301, 370, 489
MseI TTAA 3 cut(s) 180, 186, 360
MslI CAYNNNNRTG 1 cut(s) 285
MspCI CTTAAG 1 cut(s) 185
MspR9I CCNGG 3 cut(s) 36, 329, 461
MvaI CCWGG 3 cut(s) 36, 329, 461
MwoI GCNNNNNNNGC 1 cut(s) 465
NcoI CCATGG 1 cut(s) 174
NdeII GATC 5 cut(s) 74, 208, 276, 324, 506
NlaIII CATG 2 cut(s) 13, 178
NlaIV GGNNCC 2 cut(s) 210, 231
NspI RCATGY 1 cut(s) 13
PciI ACATGT 1 cut(s) 9
PfoI TCCNGGA 1 cut(s) 327
PleI GAGTC 1 cut(s) 326
PpsI GAGTC 1 cut(s) 326
PscI ACATGT 1 cut(s) 9
PshAI GACNNNNGTC 1 cut(s) 12
Psp124BI GAGCTC 1 cut(s) 384
Psp6I CCWGG 3 cut(s) 34, 327, 459
PspGI CCWGG 3 cut(s) 34, 327, 459
PspN4I GGNNCC 2 cut(s) 210, 231
PspPI GGNCC 2 cut(s) 32, 229
PsuI RGATCY 3 cut(s) 208, 276, 506
RseI CAYNNNNRTG 1 cut(s) 285
SacI GAGCTC 1 cut(s) 384
SaqAI TTAA 3 cut(s) 180, 186, 360
Sau3AI GATC 5 cut(s) 74, 208, 276, 324, 506
Sau96I GGNCC 2 cut(s) 32, 229
SchI GAGTC 1 cut(s) 326
ScrFI CCNGG 3 cut(s) 36, 329, 461
SduI GDGCHC 3 cut(s) 301, 384, 470
SinI GGWCC 1 cut(s) 32
SmiMI CAYNNNNRTG 1 cut(s) 285
SmlI CTYRAG 1 cut(s) 185
SmoI CTYRAG 1 cut(s) 185
Sse9I AATT 3 cut(s) 88, 356, 500
SsiI CCGC 4 cut(s) 206, 212, 301, 471
SspMI CTAG 1 cut(s) 338
SstI GAGCTC 1 cut(s) 384
StyD4I CCNGG 3 cut(s) 34, 327, 459
StyI CCWWGG 2 cut(s) 174, 348
TaaI ACNGT 2 cut(s) 415, 496
TaiI ACGT 1 cut(s) 401
TaqI TCGA 2 cut(s) 261, 323
TasI AATT 3 cut(s) 88, 356, 500
Tru1I TTAA 3 cut(s) 180, 186, 360
Tru9I TTAA 3 cut(s) 180, 186, 360
TscAI CASTG 1 cut(s) 301
TspRI CASTG 1 cut(s) 301
Vha464I CTTAAG 1 cut(s) 185
VpaK11BI GGWCC 1 cut(s) 32
XceI RCATGY 1 cut(s) 13
XcmI CCANNNNNNNNNTGG 1 cut(s) 301
XspI CTAG 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.