Rmu_sc0014259.1_g000015

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0014259.1
OG0006394
Physical Location & Seq
Forward (+)
66295 .. 67002
708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0014259.1_g000015.1.cds

Sequence Viewer

Length: 708 bp
atgggcaaactcaagggggcggggaagccgccgtcgccaccacacgtttccggcgaactttctcacatcgagtttgagcttcaaattcacagggacgaaaccaacgctcgcttggaggagatgcagtccaatttgcaaaattccatagccagttctttagcgggttttagatcggtgcttttggaggagatcaaacagctcactgagcttcgacaaggctcactcacgccaccaccactacctgatggcaccgcagtgccacaaccggatggatctgtgaaattcggctctctacctccacctcccgtacaaccaaagatgggagctctatccgagtcatcatcatcatcggtgttggcccgatccgaaggtatccctcctaaccccattaagccgctcacagatgctgatatactgttgggatttattaaccctgtggtttcaagtcctgtgactactatgcctgatttgagtgtcttaaacaataatctgggcaccaaagagaaaggggatactgatggtggtacctctaaaatggttactagttgtgttagtaatacggctatgactatggttgaacgtgctgtgagtgaagcacaaacaaacccattgcaatatgctgggtttggccctcctactaatactagcactggattgttcaatggtcagatccactcgcacttacaccagtcccaagcctcttattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag gag-asp_proteas rve

Protein Analysis

235

Amino Acids

24.78

Weight (kDa)

5.48

Isoelectric Point (pI)

56.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 524
AccB1I GGYRCC 3 cut(s) 248, 494, 524
AccBSI CCGCTC 1 cut(s) 397
AciI CCGC 5 cut(s) 20, 29, 161, 252, 395
AclWI GGATC 3 cut(s) 280, 357, 664
AcsI RAATTY 3 cut(s) 84, 139, 281
AfaI GTAC 2 cut(s) 309, 526
AfiI CCNNNNNNNGG 1 cut(s) 320
AflIII ACRYGT 1 cut(s) 43
AgsI TTSAA 4 cut(s) 83, 444, 578, 661
AhlI ACTAGT 1 cut(s) 542
AleI CACNNNNGTG 1 cut(s) 254
AluBI AGCT 4 cut(s) 79, 199, 208, 326
AluI AGCT 4 cut(s) 79, 199, 208, 326
Alw21I GWGCWC 1 cut(s) 328
AlwI GGATC 3 cut(s) 280, 357, 664
AlwNI CAGNNNCTG 1 cut(s) 407
AoxI GGCC 2 cut(s) 357, 628
ApoI RAATTY 3 cut(s) 84, 139, 281
Asp718I GGTACC 1 cut(s) 524
AspS9I GGNCC 2 cut(s) 358, 629
BaeGI GKGCMC 1 cut(s) 497
BanI GGYRCC 3 cut(s) 248, 494, 524
BanII GRGCYC 1 cut(s) 328
Bbv12I GWGCWC 1 cut(s) 328
BccI CCATC 4 cut(s) 239, 263, 313, 512
BceAI ACGGC 2 cut(s) 16, 576
BciVI GTATCC 2 cut(s) 383, 505
BcuI ACTAGT 1 cut(s) 542
BfaI CTAG 2 cut(s) 543, 645
BfuI GTATCC 2 cut(s) 383, 505
BisI GCNGC 2 cut(s) 29, 395
BlsI GCNGC 2 cut(s) 30, 396
BmgT120I GGNCC 2 cut(s) 358, 629
BmiI GGNNCC 3 cut(s) 250, 496, 526
BmsI GCATC 2 cut(s) 111, 394
BsaWI WCCGGW 1 cut(s) 265
BsaXI ACNNNNNCTCC 2 cut(s) 110, 140
Bsc4I CCNNNNNNNGG 1 cut(s) 320
Bse1I ACTGG 3 cut(s) 150, 655, 688
Bse3DI GCAATG 1 cut(s) 608
BseGI GGATG 1 cut(s) 274
BseLI CCNNNNNNNGG 1 cut(s) 320
BseMI GCAATG 1 cut(s) 608
BseMII CTCAG 1 cut(s) 195
BseNI ACTGG 3 cut(s) 150, 655, 688
BseRI GAGGAG 2 cut(s) 131, 200
BseSI GKGCMC 1 cut(s) 497
BseYI CCCAGC 1 cut(s) 620
BshFI GGCC 2 cut(s) 359, 630
BshNI GGYRCC 3 cut(s) 248, 494, 524
BsiHKAI GWGCWC 1 cut(s) 328
BsiSI CCGG 2 cut(s) 51, 266
BslFI GGGAC 2 cut(s) 107, 676
BslI CCNNNNNNNGG 1 cut(s) 320
BsmFI GGGAC 2 cut(s) 107, 676
BsnI GGCC 2 cut(s) 359, 630
Bsp1286I GDGCHC 2 cut(s) 328, 497
Bsp143I GATC 5 cut(s) 170, 189, 272, 362, 669
BspACI CCGC 5 cut(s) 20, 29, 161, 252, 395
BspANI GGCC 2 cut(s) 359, 630
BspCNI CTCAG 1 cut(s) 196
BspLI GGNNCC 3 cut(s) 250, 496, 526
BspPI GGATC 3 cut(s) 280, 357, 664
BspT107I GGYRCC 3 cut(s) 248, 494, 524
BsrBI CCGCTC 1 cut(s) 397
BsrDI GCAATG 1 cut(s) 608
BsrI ACTGG 3 cut(s) 150, 655, 688
BssMI GATC 5 cut(s) 170, 189, 272, 362, 669
Bst4CI ACNGT 1 cut(s) 417
BstC8I GCNNGC 1 cut(s) 109
BstDEI CTNAG 1 cut(s) 204
BstF5I GGATG 1 cut(s) 274
BstKTI GATC 5 cut(s) 173, 192, 275, 365, 672
BstMBI GATC 5 cut(s) 170, 189, 272, 362, 669
BstMWI GCNNNNNNNGC 2 cut(s) 34, 205
BstSLI GKGCMC 1 cut(s) 497
BstX2I RGATCY 2 cut(s) 272, 669
BstYI RGATCY 2 cut(s) 272, 669
BsuI GTATCC 2 cut(s) 383, 505
BsuRI GGCC 2 cut(s) 359, 630
BtsCI GGATG 1 cut(s) 274
BtsI GCAGTG 1 cut(s) 261
BtsIMutI CAGTG 3 cut(s) 201, 261, 648
Cac8I GCNNGC 1 cut(s) 109
CaiI CAGNNNCTG 1 cut(s) 407
Cfr13I GGNCC 2 cut(s) 358, 629
Csp6I GTAC 2 cut(s) 308, 525
CviQI GTAC 2 cut(s) 308, 525
DdeI CTNAG 1 cut(s) 204
DpnI GATC 5 cut(s) 172, 191, 274, 364, 671
DpnII GATC 5 cut(s) 170, 189, 272, 362, 669
Ecl136II GAGCTC 1 cut(s) 326
Eco24I GRGCYC 1 cut(s) 328
Eco53kI GAGCTC 1 cut(s) 326
EcoICRI GAGCTC 1 cut(s) 326
EcoT38I GRGCYC 1 cut(s) 328
FaiI YATR 6 cut(s) 146, 413, 461, 566, 572, 618
FaqI GGGAC 2 cut(s) 107, 676
FauI CCCGC 2 cut(s) 13, 154
Fnu4HI GCNGC 2 cut(s) 29, 395
FokI GGATG 1 cut(s) 281
FriOI GRGCYC 1 cut(s) 328
Fsp4HI GCNGC 2 cut(s) 29, 395
FspBI CTAG 2 cut(s) 543, 645
GluI GCNGC 2 cut(s) 29, 395
GsaI CCCAGC 1 cut(s) 624
HaeIII GGCC 2 cut(s) 359, 630
HapII CCGG 2 cut(s) 51, 266
HinfI GANTC 1 cut(s) 335
HpaII CCGG 2 cut(s) 51, 266
Hpy188I TCNGA 3 cut(s) 334, 367, 669
Hpy99I CGWCG 1 cut(s) 37
HpyAV CCTTC 1 cut(s) 362
HpyCH4III ACNGT 1 cut(s) 417
HpyCH4IV ACGT 2 cut(s) 45, 580
HpyCH4V TGCA 3 cut(s) 124, 136, 613
HpyF10VI GCNNNNNNNGC 2 cut(s) 34, 205
HpyF3I CTNAG 1 cut(s) 204
HpySE526I ACGT 2 cut(s) 45, 580
KpnI GGTACC 1 cut(s) 528
Kzo9I GATC 5 cut(s) 170, 189, 272, 362, 669
LmnI GCTCC 1 cut(s) 323
LweI GCATC 2 cut(s) 111, 394
MaeI CTAG 2 cut(s) 543, 645
MaeII ACGT 2 cut(s) 45, 580
MaeIII GTNAC 2 cut(s) 451, 538
MalI GATC 5 cut(s) 172, 191, 274, 364, 671
MbiI CCGCTC 1 cut(s) 397
MboI GATC 5 cut(s) 170, 189, 272, 362, 669
MflI RGATCY 2 cut(s) 272, 669
MhlI GDGCHC 2 cut(s) 328, 497
MluCI AATT 4 cut(s) 84, 130, 139, 281
MlyI GAGTC 1 cut(s) 344
MnlI CCTC 7 cut(s) 109, 178, 306, 312, 387, 538, 642
MseI TTAA 3 cut(s) 390, 429, 479
MslI CAYNNNNRTG 1 cut(s) 254
MspI CCGG 2 cut(s) 51, 266
MwoI GCNNNNNNNGC 2 cut(s) 34, 205
NdeII GATC 5 cut(s) 170, 189, 272, 362, 669
NlaIV GGNNCC 3 cut(s) 250, 496, 526
NmuCI GTSAC 1 cut(s) 451
OliI CACNNNNGTG 1 cut(s) 254
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PkrI GCNGC 2 cut(s) 30, 396
PleI GAGTC 1 cut(s) 343
PpsI GAGTC 1 cut(s) 343
Psp124BI GAGCTC 1 cut(s) 328
PspFI CCCAGC 1 cut(s) 620
PspN4I GGNNCC 3 cut(s) 250, 496, 526
PspPI GGNCC 2 cut(s) 358, 629
PstNI CAGNNNCTG 1 cut(s) 407
PsuI RGATCY 2 cut(s) 272, 669
RsaI GTAC 2 cut(s) 309, 526
RsaNI GTAC 2 cut(s) 308, 525
RseI CAYNNNNRTG 1 cut(s) 254
SacI GAGCTC 1 cut(s) 328
SaqAI TTAA 3 cut(s) 390, 429, 479
SatI GCNGC 2 cut(s) 29, 395
Sau3AI GATC 5 cut(s) 170, 189, 272, 362, 669
Sau96I GGNCC 2 cut(s) 358, 629
SchI GAGTC 1 cut(s) 344
SduI GDGCHC 2 cut(s) 328, 497
SfaNI GCATC 2 cut(s) 111, 394
SmiMI CAYNNNNRTG 1 cut(s) 254
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
SpeI ACTAGT 1 cut(s) 542
Sse9I AATT 4 cut(s) 84, 130, 139, 281
SsiI CCGC 5 cut(s) 20, 29, 161, 252, 395
SspMI CTAG 2 cut(s) 543, 645
SstI GAGCTC 1 cut(s) 328
TaaI ACNGT 1 cut(s) 417
TaiI ACGT 2 cut(s) 48, 583
TaqI TCGA 2 cut(s) 69, 211
TasI AATT 4 cut(s) 84, 130, 139, 281
TauI GCSGC 2 cut(s) 31, 397
Tru1I TTAA 3 cut(s) 390, 429, 479
Tru9I TTAA 3 cut(s) 390, 429, 479
TscAI CASTG 3 cut(s) 208, 261, 655
TseFI GTSAC 1 cut(s) 451
Tsp45I GTSAC 1 cut(s) 451
TspRI CASTG 3 cut(s) 208, 261, 655
XapI RAATTY 3 cut(s) 84, 139, 281
XcmI CCANNNNNNNNNTGG 1 cut(s) 109
XspI CTAG 2 cut(s) 543, 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.