RchiOBHm_Chr1g0357401

DNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0005182
Physical Location & Seq
Reverse (-)
49788877 .. 49792885
4009 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3735 bp
ATGTCATTCCATGGCTTCGATCCCGGGGATCGTAACAACTGGTATCAGAGCCCGCACTTGGGGCCTTTCCCGGCGAGTCCGGCTGCTTACGGCGGTGGGATTTCCGGCAGGTATTACAGCTCTCCTTATCATTCTTACGGTAGCTCTACCCATGAGTCTACCCAATTTTCATCGCAACCAAACCCCTACCCACCATTACAACTCAACCCTTCTGCAACCATCATCAATGGCCACTTACTCCCTCATCAATTTCCGTGTGTTCCTCAGACTCACTCACAGTTCTTCCCTCCTCCATATTTGCAACCGATTCATCCCATTGCTCAACAGCCGCTCACCATCACTCCTCGAAGTCCATCAATCCCTCATTCTAACCCAAATCCATGGATATATCCTCCAATTTCACAAAGCCTATGTTATGAGCCTCATTCTCAGTCCGTCTCCACCAAATCCATGACTACAACACCTATTTACCATTCTCCTCCTCCACCTCTCGCTTGTGAGCAACCACATACAATTGCAGATCCAAAACCACTCTGTCCCATACCCGAGTATGATCGAATTTCTCTTGAAACTGACAGGGTACATAATTGGGAGCTCCCAATTGTTGATTCTGGCTGTTACTCTTTCACACCAGAGCCTATGGCCAAGCTTAAGGGGGTTGAGAAACCACAATCGCCACCACGAGGTTCCGGCGAATTGCAAGTTGACAAAGATCACACAGATGCTAAGCTGCGTGAGATGCAAAAATCTTTTAAGAGTTCGATGGATACCTTCATGTCTGAATTCAGGGAGGAGCTGCGGTTGTTAAGGGTTTCGTCGCAATCTCCGGTAATCCAAGTTGATAACACACCGAAGTTTGGGTCACAACTGGATCCTACGATTGCTACGTCTCTTACAGCGTCGGGGAAGCCATTATCGTCAACTACCACTACAGCTGGAGGTAGTATTCAAATCTCGGAACCAGATTTTAATCTATCACCTCATGAAGCAATATTGGAATTGAATAATGATGTATCTGTTGTGGTGGTATCTGGAAGTAGTAGTGAAGATAATATTGATGTTGGTATGACTGAAAAACTTGTGTTAAATGTTGATAAAAATATTGTTGGAAGTGCTACTGGAGTGAAATTGTCTGATAGTCACGGTAGTGATGTTGCCAACAGTATAGTGTGGAGGAATGATGCTCAAACTCAACATACTAGCTTTAGAAATGGGTCTTCTGCAGGGTATGGAGAATTGGGATCTCATAATCATAATTTAGTTGGAAGTCTAAGAGTTGATTTCCATGAGAGAGATGAAAAAGAACTGGAAGATGAAGATGGTGAAGATGATAATGAGGATGAAGGGGAAAATGATGGTGCAGAGAGTTATGGAGACGGTTCTTTGAGTTATAGTGATGATTCTCAGAGTGTTGATGGGCAGATTGTGACAGATTCAAATGAAGAAGTGGTTATTGATGAAGAAGGAAGCAGACTACGCATTAAAAAAGAGGCATTGCTTGGAGACTCTGAGTTTATTCATGACAAAGACCACATGCTTGAAGATCATAAGCAAAGTATAATTGTGACTGATTTTCCCCAATTATTGTGGGATGAGGGAAACGTTTCTCAGGTAATGATTCTCAATCCTAAATTGGAAGCCACTTTGTTGAATCGAAACTTGGTTGAATCATTTGCGACAATAGCGGTGTCAATGCTTGATTTCTCTAAGCATGCAAGCAAACACAAGACCATTGATTTTGAGCAGTTCATATTGTATCATTTTGGTATAAGTGAATTAATGGCAACTCTTGAGGTAGCAAACTTACAAGCTTGTCTACCTAACTGGAAAGTTAGAGTAAAATTTTCAACTTCAAGTCACAAAGGAAATTTTGAATATGACCTGCATATTACAAACTTGATTGAGAGCATGAGAATAAAATTTGTTCACATGCATGAGAAGCAAAGTGGAACCAAATCATCAGCCCTGCATTGTTTTGGTTTCATTAATTCAATTAGCAATATCGGTATTCAGACCATTGTGCCATCAACTAGTCATGCCTGGGATTTTCAAACTCATCAACTTGGGTCACCCTTGCCTACTTCTGGTCATTTTGAGGTGATAAATTTTGCAAAAGGGCTTCTTGCTTTCCAGATCTATTTTTCAATTGGTACCATTGGTTCCAAGATGACAGTCATTGCTTCCATAGGTGAGATCTGCAATCAAATACTACAACCTCAAATGTGCAATTCTATATTTGCAGCAAATTCTACCAAACTGAGCCCATTTGTGCTTGTTATTCTGGACATTGAGCTACACAATCAAGAGAGTTTGTCTAGACCATTATTTGGTGCTAATCCGCTTCTATGTTCTTTGGATAGTGATGATTTCCGGTCTGCAGTGTTCAATTTTTTTCATCACTGGCTTTGCATTGTTCTTGTCCAAGCATTTGCAAAATCAGAAGGCTTGTTGAATCATGATCTAGAGGCTCCAGGACCTCATGAATGGTTGGCAGGAGCTATTGAAATTGGAAATCCTTATGCTTTGATTTTGGCTCAGAAAATGGGAGAAAAAGTTTATGTGGCCAGTGCAAGATTTGGGAAACTTCTGCCAGACCCATTTATTCCCAGCAAACTATTCTATACTGATGAAGATATTGCAAAGACTTTCCCGTGCAATTGTGAGGTGATTATTGAGGGATCCCATCTCCCATCATCTCATGATTGCAAACACTTGCCTTTTGATGCTCTGCTTCTTCTCGTACCGAGGTTGCCGGGTTCTCATATACCATTTTGTTTGTCTTCCAAAGTTGTTCAGTGCATTACTGCTGTGATTGCCTGTGTTGTGTTTCTAACTGCAGCTTATGTCCGCTACAAATTACGTGTTTGTTGTTGCACAGAAAAAGATTTAAAGCCTGCAAAGAATGGCCGCAGTGGTTCTCTTTTCCAGATATATATTGGCAAGATTCTTCATGTTGCTTCCATACTGGTAATCAATGATGAACTTGTTCAAATTAACAGAGTAGCGAACATTTGTCTTGAGCACTGCATAGTTTTTCTTGAGAAAAAAGGTGTCAAGAATGTGCACAAATGGGGTTGGTACAAACTCCGAATTGTAGCAAATGCTATAATGGGAGTTGATTACTTTTTTCAAGCTGCAACTTTGAAAGAATTCAGACAAGAGGATGTAGGAAATGGTATTCCACTAGTCTTCGCACCTAGTATACAAGACAATGAAGAAGATGGTGGACTTATTGTAAATGATGAGGATGTGAGAGCTGGTGTTGTTTTACATGATGATGGAATAATGATTGTGGAAGATGATATTACTGATGGGGACAAGGAACCTACTATTCCATTGCTTTGGAATATGTTTGTTCATTTGCAGCTGCCTCTTTTGGTCAAGAAAGCAACATTAGCTGATGAAACATGCAAAATTCAAGAAACTATGGATGGCTCCATTAATGCTAATTCTCCTCCAATATTTTCAGGCTTTCATGATCAAGCTCTGATCATGATTTTGTACTCCTTGCAGTTGATTAGAGCCTGGCACAAAAGCAGGGGTTACTTGCCATTTGAAGACTCCAATTATCCAACAATGCTTTGGTTCAGTTACATTTTCCTTGGCCTTGGAGTTACTCTGTGTTCAGGACATATTGCTGCAGACACTGCAAATGGTTATGGGTTTCAAATGATATCTTGCAAAGGTGCCCAGATTTTGATTCAGAACATGAAACTTGAGGACAAGTTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000182 GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003712 GO:0003714 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006091 GO:0006139 GO:0006351 GO:0006355 GO:0006360 GO:0006725 GO:0006807 GO:0006915 GO:0006950 GO:0007154 GO:0007165 GO:0007623 GO:0008134 GO:0008150 GO:0008152 GO:0008219 GO:0008270 GO:0009058 GO:0009059 GO:0009267 GO:0009303 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010629 GO:0010941 GO:0010942 GO:0012501 GO:0015980 GO:0016070 GO:0016072 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022900 GO:0022904 GO:0023052 GO:0031074 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032501 GO:0032774 GO:0032922 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035556 GO:0042149 GO:0042564 GO:0042594 GO:0042790 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045333 GO:0045787 GO:0045892 GO:0045934 GO:0046483 GO:0046872 GO:0046914 GO:0048511 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0051726 GO:0055114 GO:0060255 GO:0065007 GO:0070013 GO:0070888 GO:0071156 GO:0071158 GO:0071496 GO:0071704 GO:0072331 GO:0072332 GO:0080090 GO:0090068 GO:0090304 GO:0097159 GO:0097190 GO:0097193 GO:0097659 GO:0098781 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2000209 GO:2000210 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_phi

Protein Analysis

1244

Amino Acids

137.84

Weight (kDa)

5.3

Isoelectric Point (pI)

44.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 99, 1890
Acc65I GGTACC 1 cut(s) 2150
AccB1I GGYRCC 2 cut(s) 2150, 3686
AccB7I CCANNNNNTGG 1 cut(s) 2327
AccBSI CCGCTC 1 cut(s) 331
AccI GTMKAC 3 cut(s) 158, 1816, 3202
AciI CCGC 8 cut(s) 53, 93, 329, 799, 1685, 2339, 2848, 2908
AclI AACGTT 1 cut(s) 1602
AclWI GGATC 8 cut(s) 14, 36, 515, 866, 879, 1249, 2673, 2686
AcoI YGGCCR 4 cut(s) 229, 642, 2562, 2905
AcsI RAATTY 9 cut(s) 558, 782, 1841, 1867, 1919, 2104, 2245, 3149, 3414
AfaI GTAC 5 cut(s) 582, 2152, 2742, 3080, 3503
AfiI CCNNNNNNNGG 6 cut(s) 58, 59, 683, 857, 2084, 2327
AflII CTTAAG 1 cut(s) 650
AflIII ACRYGT 1 cut(s) 2860
AhlI ACTAGT 2 cut(s) 2030, 3184
AjnI CCWGG 3 cut(s) 2039, 2470, 3524
AjuI GAANNNNNNNTTGG 4 cut(s) 592, 624, 1643, 1675
AleI CACNNNNGTG 1 cut(s) 1146
Alw21I GWGCWC 3 cut(s) 597, 3024, 3066
Alw26I GTCTC 4 cut(s) 442, 894, 1368, 1497
Alw44I GTGCAC 1 cut(s) 3062
AlwI GGATC 8 cut(s) 14, 36, 515, 866, 879, 1249, 2673, 2686
AlwNI CAGNNNCTG 1 cut(s) 3647
Ama87I CYCGRG 2 cut(s) 23, 545
AoxI GGCC 6 cut(s) 62, 229, 642, 2562, 2905, 3604
ApaLI GTGCAC 1 cut(s) 3062
ApeKI GCWGC 9 cut(s) 83, 730, 796, 2240, 2837, 3134, 3364, 3367, 3638
ApoI RAATTY 9 cut(s) 558, 782, 1841, 1867, 1919, 2104, 2245, 3149, 3414
ArsI GACNNNNNNTTYG 2 cut(s) 845, 877
AseI ATTAAT 3 cut(s) 1778, 1986, 3441
Asp700I GAANNNNTTC 3 cut(s) 1603, 2985, 3149
Asp718I GGTACC 1 cut(s) 2150
AspS9I GGNCC 2 cut(s) 62, 2474
AsuC2I CCSGG 4 cut(s) 24, 25, 71, 2754
AsuHPI GGTGA 7 cut(s) 325, 969, 1334, 2061, 2110, 2201, 2677
AvaI CYCGRG 2 cut(s) 23, 545
AvaII GGWCC 1 cut(s) 2474
BaeGI GKGCMC 2 cut(s) 3066, 3691
BalI TGGCCA 3 cut(s) 231, 644, 2564
BamHI GGATCC 2 cut(s) 871, 2678
BanI GGYRCC 2 cut(s) 2150, 3686
BanII GRGCYC 3 cut(s) 53, 597, 2264
BauI CACGAG 1 cut(s) 681
BbsI GAAGAC 4 cut(s) 1209, 2772, 3181, 3564
Bbv12I GWGCWC 3 cut(s) 597, 3024, 3066
BbvI GCAGC 9 cut(s) 70, 717, 783, 2252, 2849, 3121, 3354, 3376, 3625
BceAI ACGGC 1 cut(s) 106
BciT130I CCWGG 3 cut(s) 2041, 2472, 3526
BciVI GTATCC 1 cut(s) 760
BclI TGATCA 2 cut(s) 3478, 3489
BcnI CCSGG 4 cut(s) 24, 25, 71, 2754
BcoDI GTCTC 4 cut(s) 442, 894, 1368, 1497
BcuI ACTAGT 2 cut(s) 2030, 3184
BfaI CTAG 6 cut(s) 1200, 2031, 2316, 2462, 3185, 3198
BfmI CTRYAG 5 cut(s) 930, 1221, 2376, 2835, 3639
BfrI CTTAAG 1 cut(s) 650
BfuAI ACCTGC 2 cut(s) 99, 1890
BfuI GTATCC 1 cut(s) 760
BglII AGATCT 2 cut(s) 2133, 2193
BlpI GCTNAGC 1 cut(s) 726
Bme1390I CCNGG 7 cut(s) 24, 25, 71, 2041, 2472, 2754, 3526
Bme18I GGWCC 1 cut(s) 2474
BmeT110I CYCGRG 2 cut(s) 23, 545
BmgT120I GGNCC 2 cut(s) 62, 2474
BmrFI CCNGG 7 cut(s) 24, 25, 71, 2041, 2472, 2754, 3526
BmsI GCATC 4 cut(s) 712, 729, 1171, 2713
BpiI GAAGAC 4 cut(s) 1209, 2772, 3181, 3564
BpmI CTGGAG 3 cut(s) 957, 1140, 2454
Bpu1102I GCTNAGC 1 cut(s) 726
BpuEI CTTGAG 3 cut(s) 1811, 3038, 3059
BpuMI CCSGG 4 cut(s) 24, 25, 71, 2754
BsaAI YACGTR 1 cut(s) 2861
BsaBI GATNNNNATC 1 cut(s) 969
BsaJI CCNNGG 8 cut(s) 10, 23, 24, 380, 2040, 2744, 3601, 3607
BsaWI WCCGGW 2 cut(s) 826, 2370
BsaXI ACNNNNNCTCC 6 cut(s) 325, 355, 785, 815, 2538, 2568
Bsc4I CCNNNNNNNGG 6 cut(s) 58, 59, 683, 857, 2084, 2327
Bse1I ACTGG 8 cut(s) 44, 873, 1123, 1311, 1829, 2405, 2565, 2970
Bse3DI GCAATG 4 cut(s) 315, 1493, 2175, 3335
Bse8I GATNNNNATC 1 cut(s) 969
BseBI CCWGG 3 cut(s) 2041, 2472, 3526
BseDI CCNNGG 8 cut(s) 10, 23, 24, 380, 2040, 2744, 3601, 3607
BseGI GGATG 6 cut(s) 310, 1345, 1597, 3169, 3253, 3436
BseJI GATNNNNATC 1 cut(s) 969
BseLI CCNNNNNNNGG 6 cut(s) 58, 59, 683, 857, 2084, 2327
BseMI GCAATG 4 cut(s) 315, 1493, 2175, 3335
BseMII CTCAG 7 cut(s) 278, 443, 1418, 1500, 1622, 2249, 2549
BseNI ACTGG 8 cut(s) 44, 873, 1123, 1311, 1829, 2405, 2565, 2970
BseRI GAGGAG 6 cut(s) 279, 333, 468, 471, 806, 3444
BseSI GKGCMC 2 cut(s) 3066, 3691
BseXI GCAGC 9 cut(s) 70, 717, 783, 2252, 2849, 3121, 3354, 3376, 3625
BseYI CCCAGC 1 cut(s) 2606
BsgI GTGCAG 1 cut(s) 1380
BshFI GGCC 6 cut(s) 64, 231, 644, 2564, 2907, 3606
BshNI GGYRCC 2 cut(s) 2150, 3686
BsiHKAI GWGCWC 3 cut(s) 597, 3024, 3066
BsiHKCI CYCGRG 2 cut(s) 23, 545
BsiSI CCGG 8 cut(s) 24, 71, 80, 105, 690, 827, 2371, 2753
BslFI GGGAC 2 cut(s) 522, 3329
BslI CCNNNNNNNGG 6 cut(s) 58, 59, 683, 857, 2084, 2327
BsmAI GTCTC 4 cut(s) 442, 894, 1368, 1497
BsmBI CGTCTC 3 cut(s) 442, 894, 1368
BsmFI GGGAC 2 cut(s) 522, 3329
BsnI GGCC 6 cut(s) 64, 231, 644, 2564, 2907, 3606
BsoBI CYCGRG 2 cut(s) 23, 545
Bsp1286I GDGCHC 6 cut(s) 53, 597, 2264, 3024, 3066, 3691
Bsp1720I GCTNAGC 1 cut(s) 726
Bsp19I CCATGG 2 cut(s) 10, 380
BspACI CCGC 8 cut(s) 53, 93, 329, 799, 1685, 2339, 2848, 2908
BspANI GGCC 6 cut(s) 64, 231, 644, 2564, 2907, 3606
BspCNI CTCAG 7 cut(s) 277, 442, 1417, 1501, 1621, 2250, 2548
BspHI TCATGA 7 cut(s) 982, 1519, 2455, 2479, 2698, 3475, 3492
BspMAI CTGCAG 4 cut(s) 1225, 2380, 2839, 3643
BspMI ACCTGC 2 cut(s) 99, 1890
BspPI GGATC 8 cut(s) 14, 36, 515, 866, 879, 1249, 2673, 2686
BspT107I GGYRCC 2 cut(s) 2150, 3686
BspTI CTTAAG 1 cut(s) 650
BsrBI CCGCTC 1 cut(s) 331
BsrDI GCAATG 4 cut(s) 315, 1493, 2175, 3335
BsrI ACTGG 8 cut(s) 44, 873, 1123, 1311, 1829, 2405, 2565, 2970
BssECI CCNNGG 8 cut(s) 10, 23, 24, 380, 2040, 2744, 3601, 3607
BssNAI GTATAC 1 cut(s) 3203
BssSI CACGAG 1 cut(s) 681
BssT1I CCWWGG 4 cut(s) 10, 380, 3601, 3607
Bst1107I GTATAC 1 cut(s) 3203
Bst2BI CACGAG 1 cut(s) 681
Bst2UI CCWGG 3 cut(s) 2041, 2472, 3526
Bst4CI ACNGT 6 cut(s) 140, 279, 1145, 1163, 1379, 2173
BstAFI CTTAAG 1 cut(s) 650
BstAPI GCANNNNNTGC 1 cut(s) 3647
BstBAI YACGTR 1 cut(s) 2861
BstC8I GCNNGC 4 cut(s) 53, 1713, 1717, 2895
BstDSI CCRYGG 2 cut(s) 10, 380
BstEII GGTNACC 1 cut(s) 2067
BstF5I GGATG 6 cut(s) 310, 1345, 1597, 3169, 3253, 3436
BstMAI GTCTC 4 cut(s) 442, 894, 1368, 1497
BstMWI GCNNNNNNNGC 9 cut(s) 61, 80, 739, 1476, 1682, 1939, 2813, 3395, 3647
BstNI CCWGG 3 cut(s) 2041, 2472, 3526
BstNSI RCATGY 4 cut(s) 1537, 1715, 1933, 3411
BstPI GGTNACC 1 cut(s) 2067
BstSCI CCNGG 7 cut(s) 22, 23, 69, 2039, 2470, 2752, 3524
BstSFI CTRYAG 5 cut(s) 930, 1221, 2376, 2835, 3639
BstSLI GKGCMC 2 cut(s) 3066, 3691
BstV1I GCAGC 9 cut(s) 70, 717, 783, 2252, 2849, 3121, 3354, 3376, 3625
BstV2I GAAGAC 4 cut(s) 1209, 2772, 3181, 3564
BstX2I RGATCY 6 cut(s) 520, 871, 1241, 2133, 2193, 2678
BstXI CCANNNNNNTGG 2 cut(s) 381, 3342
BstYI RGATCY 6 cut(s) 520, 871, 1241, 2133, 2193, 2678
BstZ17I GTATAC 1 cut(s) 3203
BsuI GTATCC 1 cut(s) 760
BsuRI GGCC 6 cut(s) 64, 231, 644, 2564, 2907, 3606
BtgI CCRYGG 2 cut(s) 10, 380
BtgZI GCGATG 1 cut(s) 156
BtsCI GGATG 6 cut(s) 310, 1345, 1597, 3169, 3253, 3436
BtsI GCAGTG 4 cut(s) 2385, 2917, 3022, 3645
BtsIMutI CAGTG 7 cut(s) 2385, 2398, 2572, 2801, 2917, 3022, 3645
BveI ACCTGC 2 cut(s) 99, 1890
Cac8I GCNNGC 4 cut(s) 53, 1713, 1717, 2895
CaiI CAGNNNCTG 1 cut(s) 3647
CciI TCATGA 7 cut(s) 982, 1519, 2455, 2479, 2698, 3475, 3492
Cfr13I GGNCC 2 cut(s) 62, 2474
Cfr9I CCCGGG 1 cut(s) 23
CseI GACGC 1 cut(s) 888
Csp6I GTAC 5 cut(s) 581, 2151, 2741, 3079, 3502
CspCI CAANNNNNGTGG 2 cut(s) 1568, 1603
CviQI GTAC 5 cut(s) 581, 2151, 2741, 3079, 3502
DraI TTTAAA 1 cut(s) 2889
EaeI YGGCCR 4 cut(s) 229, 642, 2562, 2905
Ecl136II GAGCTC 1 cut(s) 595
Eco130I CCWWGG 4 cut(s) 10, 380, 3601, 3607
Eco24I GRGCYC 3 cut(s) 53, 597, 2264
Eco32I GATATC 1 cut(s) 3675
Eco47I GGWCC 1 cut(s) 2474
Eco53kI GAGCTC 1 cut(s) 595
Eco88I CYCGRG 2 cut(s) 23, 545
Eco91I GGTNACC 1 cut(s) 2067
EcoICRI GAGCTC 1 cut(s) 595
EcoO109I RGGNCCY 2 cut(s) 62, 2474
EcoO65I GGTNACC 1 cut(s) 2067
EcoRI GAATTC 2 cut(s) 782, 3149
EcoRII CCWGG 3 cut(s) 2039, 2470, 3524
EcoRV GATATC 1 cut(s) 3675
EcoT14I CCWWGG 4 cut(s) 10, 380, 3601, 3607
EcoT22I ATGCAT 1 cut(s) 1935
EcoT38I GRGCYC 3 cut(s) 53, 597, 2264
ErhI CCWWGG 4 cut(s) 10, 380, 3601, 3607
Esp3I CGTCTC 3 cut(s) 442, 894, 1368
FalI AAGNNNNNCTT 2 cut(s) 2106, 2138
FaqI GGGAC 2 cut(s) 522, 3329
FauI CCCGC 1 cut(s) 60
FbaI TGATCA 2 cut(s) 3478, 3489
FblI GTMKAC 3 cut(s) 158, 1816, 3202
FokI GGATG 6 cut(s) 297, 1352, 1604, 3176, 3260, 3443
FriOI GRGCYC 3 cut(s) 53, 597, 2264
FspBI CTAG 6 cut(s) 1200, 2031, 2316, 2462, 3185, 3198
GsaI CCCAGC 1 cut(s) 2610
GsuI CTGGAG 3 cut(s) 957, 1140, 2454
HaeIII GGCC 6 cut(s) 64, 231, 644, 2564, 2907, 3606
HapII CCGG 8 cut(s) 24, 71, 80, 105, 690, 827, 2371, 2753
HgaI GACGC 1 cut(s) 888
HincII GTYRAC 2 cut(s) 706, 921
HindII GTYRAC 2 cut(s) 706, 921
HindIII AAGCTT 2 cut(s) 647, 1809
HpaII CCGG 8 cut(s) 24, 71, 80, 105, 690, 827, 2371, 2753
HphI GGTGA 7 cut(s) 325, 969, 1334, 2061, 2110, 2201, 2677
Hpy166II GTNNAC 8 cut(s) 159, 706, 921, 1817, 1927, 3064, 3203, 3227
Hpy8I GTNNAC 8 cut(s) 159, 706, 921, 1817, 1927, 3064, 3203, 3227
Hpy99I CGWCG 2 cut(s) 820, 904
HpyAV CCTTC 5 cut(s) 219, 781, 1337, 1457, 2435
HpyCH4III ACNGT 6 cut(s) 140, 279, 1145, 1163, 1379, 2173
HpyCH4IV ACGT 3 cut(s) 887, 1602, 2860
HpyF10VI GCNNNNNNNGC 9 cut(s) 61, 80, 739, 1476, 1682, 1939, 2813, 3395, 3647
HpySE526I ACGT 3 cut(s) 887, 1602, 2860
KpnI GGTACC 1 cut(s) 2154
Ksp22I TGATCA 2 cut(s) 3478, 3489
LmnI GCTCC 6 cut(s) 592, 600, 793, 2473, 2495, 3440
Lsp1109I GCAGC 9 cut(s) 70, 717, 783, 2252, 2849, 3121, 3354, 3376, 3625
LweI GCATC 4 cut(s) 712, 729, 1171, 2713
MaeI CTAG 6 cut(s) 1200, 2031, 2316, 2462, 3185, 3198
MaeII ACGT 3 cut(s) 887, 1602, 2860
MbiI CCGCTC 1 cut(s) 331
MfeI CAATTG 4 cut(s) 513, 600, 2145, 2656
MflI RGATCY 6 cut(s) 520, 871, 1241, 2133, 2193, 2678
MhlI GDGCHC 6 cut(s) 53, 597, 2264, 3024, 3066, 3691
MlsI TGGCCA 3 cut(s) 231, 644, 2564
MluNI TGGCCA 3 cut(s) 231, 644, 2564
MlyI GAGTC 5 cut(s) 85, 164, 262, 1499, 3554
MmeI TCCRAC 3 cut(s) 1087, 1243, 3596
Mox20I TGGCCA 3 cut(s) 231, 644, 2564
Mph1103I ATGCAT 1 cut(s) 1935
MroXI GAANNNNTTC 3 cut(s) 1603, 2985, 3149
MscI TGGCCA 3 cut(s) 231, 644, 2564
MslI CAYNNNNRTG 4 cut(s) 720, 1146, 1932, 3276
Msp20I TGGCCA 3 cut(s) 231, 644, 2564
MspA1I CMGCKG 2 cut(s) 935, 3367
MspCI CTTAAG 1 cut(s) 650
MspI CCGG 8 cut(s) 24, 71, 80, 105, 690, 827, 2371, 2753
MspR9I CCNGG 7 cut(s) 24, 25, 71, 2041, 2472, 2754, 3526
MunI CAATTG 4 cut(s) 513, 600, 2145, 2656
MvaI CCWGG 3 cut(s) 2041, 2472, 3526
MwoI GCNNNNNNNGC 9 cut(s) 61, 80, 739, 1476, 1682, 1939, 2813, 3395, 3647
NciI CCSGG 4 cut(s) 24, 25, 71, 2754
NcoI CCATGG 2 cut(s) 10, 380
NmuCI GTSAC 6 cut(s) 861, 1139, 1426, 1564, 1856, 2067
NsiI ATGCAT 1 cut(s) 1935
NspI RCATGY 4 cut(s) 1537, 1715, 1933, 3411
OliI CACNNNNGTG 1 cut(s) 1146
PaeI GCATGC 1 cut(s) 1715
PagI TCATGA 7 cut(s) 982, 1519, 2455, 2479, 2698, 3475, 3492
PcsI WCGNNNNNNNCGW 1 cut(s) 884
PdmI GAANNNNTTC 3 cut(s) 1603, 2985, 3149
PflMI CCANNNNNTGG 1 cut(s) 2327
PfoI TCCNGGA 1 cut(s) 2470
PleI GAGTC 5 cut(s) 84, 163, 262, 1499, 3554
PpsI GAGTC 5 cut(s) 84, 163, 262, 1499, 3554
Ppu21I YACGTR 1 cut(s) 2861
PpuMI RGGWCCY 1 cut(s) 2474
PshBI ATTAAT 3 cut(s) 1778, 1986, 3441
Psp124BI GAGCTC 1 cut(s) 597
Psp1406I AACGTT 1 cut(s) 1602
Psp5II RGGWCCY 1 cut(s) 2474
Psp6I CCWGG 3 cut(s) 2039, 2470, 3524
PspEI GGTNACC 1 cut(s) 2067
PspFI CCCAGC 1 cut(s) 2606
PspGI CCWGG 3 cut(s) 2039, 2470, 3524
PspPI GGNCC 2 cut(s) 62, 2474
PspPPI RGGWCCY 1 cut(s) 2474
PsrI GAACNNNNNNTAC 2 cut(s) 2143, 2175
PstI CTGCAG 4 cut(s) 1225, 2380, 2839, 3643
PstNI CAGNNNCTG 1 cut(s) 3647
PsuI RGATCY 6 cut(s) 520, 871, 1241, 2133, 2193, 2678
PvuII CAGCTG 2 cut(s) 935, 3367
RsaI GTAC 5 cut(s) 582, 2152, 2742, 3080, 3503
RsaNI GTAC 5 cut(s) 581, 2151, 2741, 3079, 3502
RseI CAYNNNNRTG 4 cut(s) 720, 1146, 1932, 3276
SacI GAGCTC 1 cut(s) 597
Sau96I GGNCC 2 cut(s) 62, 2474
SchI GAGTC 5 cut(s) 85, 164, 262, 1499, 3554
ScrFI CCNGG 7 cut(s) 24, 25, 71, 2041, 2472, 2754, 3526
SduI GDGCHC 6 cut(s) 53, 597, 2264, 3024, 3066, 3691
SfaNI GCATC 4 cut(s) 712, 729, 1171, 2713
SfcI CTRYAG 5 cut(s) 930, 1221, 2376, 2835, 3639
SinI GGWCC 1 cut(s) 2474
SmaI CCCGGG 1 cut(s) 25
SmiMI CAYNNNNRTG 4 cut(s) 720, 1146, 1932, 3276
SmlI CTYRAG 5 cut(s) 650, 1790, 3017, 3038, 3716
SmoI CTYRAG 5 cut(s) 650, 1790, 3017, 3038, 3716
SpeI ACTAGT 2 cut(s) 2030, 3184
SphI GCATGC 1 cut(s) 1715
SsiI CCGC 8 cut(s) 53, 93, 329, 799, 1685, 2339, 2848, 2908
SspI AATATT 4 cut(s) 993, 1054, 1102, 3462
SspMI CTAG 6 cut(s) 1200, 2031, 2316, 2462, 3185, 3198
SstI GAGCTC 1 cut(s) 597
StyD4I CCNGG 7 cut(s) 22, 23, 69, 2039, 2470, 2752, 3524
StyI CCWWGG 4 cut(s) 10, 380, 3601, 3607
TaaI ACNGT 6 cut(s) 140, 279, 1145, 1163, 1379, 2173
TaiI ACGT 3 cut(s) 890, 1605, 2863
TaqI TCGA 5 cut(s) 18, 346, 556, 761, 1654
TatI WGTACW 1 cut(s) 3501
TauI GCSGC 2 cut(s) 331, 2910
TscAI CASTG 7 cut(s) 2385, 2405, 2572, 2801, 2917, 3029, 3652
TseFI GTSAC 6 cut(s) 861, 1139, 1426, 1564, 1856, 2067
TseI GCWGC 9 cut(s) 83, 730, 796, 2240, 2837, 3134, 3364, 3367, 3638
Tsp45I GTSAC 6 cut(s) 861, 1139, 1426, 1564, 1856, 2067
TspGWI ACGGA 2 cut(s) 243, 424
TspMI CCCGGG 1 cut(s) 23
TspRI CASTG 7 cut(s) 2385, 2405, 2572, 2801, 2917, 3029, 3652
Van91I CCANNNNNTGG 1 cut(s) 2327
Vha464I CTTAAG 1 cut(s) 650
VneI GTGCAC 1 cut(s) 3062
VpaK11BI GGWCC 1 cut(s) 2474
VspI ATTAAT 3 cut(s) 1778, 1986, 3441
XapI RAATTY 9 cut(s) 558, 782, 1841, 1867, 1919, 2104, 2245, 3149, 3414
XbaI TCTAGA 2 cut(s) 2315, 2461
XceI RCATGY 4 cut(s) 1537, 1715, 1933, 3411
XcmI CCANNNNNNNNNTGG 2 cut(s) 2933, 3579
XmaI CCCGGG 1 cut(s) 23
XmiI GTMKAC 3 cut(s) 158, 1816, 3202
XmnI GAANNNNTTC 3 cut(s) 1603, 2985, 3149
XspI CTAG 6 cut(s) 1200, 2031, 2316, 2462, 3185, 3198
Zsp2I ATGCAT 1 cut(s) 1935
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.