RchiOBHm_Chr0c20g0500171

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_chinensis
Unknown
OG0003358
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 825 bp
ATGTTAATCGACAGTGGGGTGAACCTAAGAATTGTTCAAACCAATCACATCTTCTTCTTTTTTTTTTTTTTTTTTTTTTTTTGTGGTCAAAATTCCAAAATTGTGCTATTTTTTTTTTTTGTCGATCAGGATGAATTATTGTGGGCTGCAGCATGGCTTCATCGTGCTACTGATGATAAATCGTACCTAGATTACCTTGGTCAAGCAGGCAATACTGGTGGTGCAAGAACCGAGTTCTCTTGGGATGACAAGTTTGTTGGCGCCCAGGTCTTGGTTGCAAAGGCAGAGCAGTTCATTTGCTCTTGCATTCAGAAGGGTAACAGCAACGTGAAGAAGACGCCCGGTGGGTTGTTATGGTTTTTGCCATTTAACAATCTTCAGTATACTACAACTGCTACACTCATTGCAACTGTTTACTCTGAATACCTATCCCCAAAACGTGCCTCCATTCAATGTAGTGGTGGCATTGTTCAGTCTTCTGCTCTACTTGACTTGGCCCGATCACAGGTGGACTATATCCTGGGTTCAAATCCTAATGGTATGAGCTACATGGTTGGATTTGGGTCAAAGTATCCAACTCAAGTTCATCATAGAGGAGCATCCATTGTTTCAATCAAGAAAGATCCATCTCCAGTTGCATGCCAAGCTGGCTTCGACGCTTGGTTTAACATGAACGCCCCTAATCCGAATGTATTGGATGGAGCAATAGTTGGAGGACCCAATGATAGTGATGGTTATACGGATTCAAGATCCAACTTCCAAATGGCTGAAGCAGCAACTGTTAATACTGCACCACTAGTTGGTGTCCTAGCCAAGCTTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

274

Amino Acids

29.99

Weight (kDa)

6.05

Isoelectric Point (pI)

30.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 42 - 270 4.6e-61 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 260
AccB7I CCANNNNNTGG 2 cut(s) 271, 800
AccI GTMKAC 1 cut(s) 383
AclWI GGATC 2 cut(s) 617, 744
AcsI RAATTY 1 cut(s) 91
AcuI CTGAAG 2 cut(s) 362, 789
AcyI GRCGYC 2 cut(s) 261, 338
AfaI GTAC 1 cut(s) 185
AfiI CCNNNNNNNGG 3 cut(s) 271, 505, 800
AgsI TTSAA 5 cut(s) 38, 452, 528, 612, 747
AhlI ACTAGT 1 cut(s) 796
AjnI CCWGG 2 cut(s) 264, 519
AjuI GAANNNNNNNTTGG 2 cut(s) 35, 67
AluBI AGCT 3 cut(s) 546, 647, 817
AluI AGCT 3 cut(s) 546, 647, 817
AlwI GGATC 2 cut(s) 617, 744
AlwNI CAGNNNCTG 1 cut(s) 779
AoxI GGCC 1 cut(s) 495
ApeKI GCWGC 3 cut(s) 146, 149, 773
ApoI RAATTY 1 cut(s) 91
AspLEI GCGC 1 cut(s) 263
AspS9I GGNCC 2 cut(s) 496, 716
AsuC2I CCSGG 1 cut(s) 342
AsuHPI GGTGA 1 cut(s) 31
AvaII GGWCC 1 cut(s) 716
BanI GGYRCC 1 cut(s) 260
BbsI GAAGAC 2 cut(s) 341, 468
BbvI GCAGC 3 cut(s) 133, 161, 785
BccI CCATC 3 cut(s) 634, 692, 725
BciT130I CCWGG 2 cut(s) 266, 521
BciVI GTATCC 1 cut(s) 582
BcnI CCSGG 1 cut(s) 342
BcuI ACTAGT 1 cut(s) 796
BfaI CTAG 3 cut(s) 188, 797, 809
BfmI CTRYAG 1 cut(s) 147
BfoI RGCGCY 1 cut(s) 264
BfuI GTATCC 1 cut(s) 582
BglI GCCNNNNNGGC 1 cut(s) 648
BisI GCNGC 3 cut(s) 147, 150, 774
BlsI GCNGC 3 cut(s) 148, 151, 775
Bme1390I CCNGG 3 cut(s) 266, 342, 521
Bme18I GGWCC 1 cut(s) 716
BmgT120I GGNCC 2 cut(s) 496, 716
BmiI GGNNCC 2 cut(s) 262, 718
BmrFI CCNGG 3 cut(s) 266, 342, 521
BmsI GCATC 1 cut(s) 608
BpiI GAAGAC 2 cut(s) 341, 468
BpmI CTGGAG 1 cut(s) 615
BpuEI CTTGAG 1 cut(s) 564
BpuMI CCSGG 1 cut(s) 342
BsaHI GRCGYC 2 cut(s) 261, 338
BsaJI CCNNGG 3 cut(s) 196, 264, 520
BsaXI ACNNNNNCTCC 2 cut(s) 693, 723
Bsc4I CCNNNNNNNGG 3 cut(s) 271, 505, 800
Bse1I ACTGG 2 cut(s) 220, 632
Bse3DI GCAATG 1 cut(s) 402
BseBI CCWGG 2 cut(s) 266, 521
BseDI CCNNGG 3 cut(s) 196, 264, 520
BseGI GGATG 4 cut(s) 136, 250, 599, 703
BseLI CCNNNNNNNGG 3 cut(s) 271, 505, 800
BseMI GCAATG 1 cut(s) 402
BseNI ACTGG 2 cut(s) 220, 632
BseRI GAGGAG 1 cut(s) 609
BseXI GCAGC 3 cut(s) 133, 161, 785
BsgI GTGCAG 1 cut(s) 774
BshFI GGCC 1 cut(s) 497
BshNI GGYRCC 1 cut(s) 260
BsiSI CCGG 1 cut(s) 342
BslI CCNNNNNNNGG 3 cut(s) 271, 505, 800
BsmI GAATGC 1 cut(s) 306
BsnI GGCC 1 cut(s) 497
Bsp143I GATC 4 cut(s) 124, 500, 622, 749
BspANI GGCC 1 cut(s) 497
BspLI GGNNCC 2 cut(s) 262, 718
BspMAI CTGCAG 1 cut(s) 151
BspPI GGATC 2 cut(s) 617, 744
BspT107I GGYRCC 1 cut(s) 260
BsrDI GCAATG 1 cut(s) 402
BsrI ACTGG 2 cut(s) 220, 632
BssECI CCNNGG 3 cut(s) 196, 264, 520
BssMI GATC 4 cut(s) 124, 500, 622, 749
BssNAI GTATAC 1 cut(s) 384
BssNI GRCGYC 2 cut(s) 261, 338
BssT1I CCWWGG 1 cut(s) 196
Bst1107I GTATAC 1 cut(s) 384
Bst2UI CCWGG 2 cut(s) 266, 521
Bst4CI ACNGT 3 cut(s) 14, 412, 781
BstACI GRCGYC 2 cut(s) 261, 338
BstC8I GCNNGC 4 cut(s) 208, 640, 649, 819
BstDEI CTNAG 1 cut(s) 26
BstF5I GGATG 4 cut(s) 136, 250, 599, 703
BstH2I RGCGCY 1 cut(s) 264
BstHHI GCGC 1 cut(s) 263
BstKTI GATC 4 cut(s) 127, 503, 625, 752
BstMBI GATC 4 cut(s) 124, 500, 622, 749
BstMWI GCNNNNNNNGC 4 cut(s) 644, 648, 773, 818
BstNI CCWGG 2 cut(s) 266, 521
BstNSI RCATGY 1 cut(s) 642
BstSCI CCNGG 3 cut(s) 264, 340, 519
BstSFI CTRYAG 1 cut(s) 147
BstV1I GCAGC 3 cut(s) 133, 161, 785
BstV2I GAAGAC 2 cut(s) 341, 468
BstX2I RGATCY 2 cut(s) 622, 749
BstYI RGATCY 2 cut(s) 622, 749
BstZ17I GTATAC 1 cut(s) 384
BsuI GTATCC 1 cut(s) 582
BsuRI GGCC 1 cut(s) 497
BtsCI GGATG 4 cut(s) 136, 250, 599, 703
BtsIMutI CAGTG 1 cut(s) 19
Cac8I GCNNGC 4 cut(s) 208, 640, 649, 819
CaiI CAGNNNCTG 1 cut(s) 779
CfoI GCGC 1 cut(s) 263
Cfr13I GGNCC 2 cut(s) 496, 716
CseI GACGC 2 cut(s) 346, 665
Csp6I GTAC 1 cut(s) 184
CspCI CAANNNNNGTGG 2 cut(s) 199, 234
CviAII CATG 4 cut(s) 153, 550, 639, 670
CviJI RGCY 9 cut(s) 146, 157, 497, 546, 647, 651, 767, 812, 817
CviKI_1 RGCY 9 cut(s) 146, 157, 497, 546, 647, 651, 767, 812, 817
CviQI GTAC 1 cut(s) 184
DdeI CTNAG 1 cut(s) 26
DinI GGCGCC 1 cut(s) 262
DpnI GATC 4 cut(s) 126, 502, 624, 751
DpnII GATC 4 cut(s) 124, 500, 622, 749
Eco130I CCWWGG 1 cut(s) 196
Eco47I GGWCC 1 cut(s) 716
Eco57I CTGAAG 2 cut(s) 362, 789
EcoO109I RGGNCCY 1 cut(s) 716
EcoRII CCWGG 2 cut(s) 264, 519
EcoT14I CCWWGG 1 cut(s) 196
EgeI GGCGCC 1 cut(s) 262
EheI GGCGCC 1 cut(s) 262
ErhI CCWWGG 1 cut(s) 196
FaeI CATG 4 cut(s) 156, 553, 642, 673
FatI CATG 4 cut(s) 152, 549, 638, 669
FblI GTMKAC 1 cut(s) 383
Fnu4HI GCNGC 3 cut(s) 147, 150, 774
FokI GGATG 4 cut(s) 143, 257, 586, 710
Fsp4HI GCNGC 3 cut(s) 147, 150, 774
FspBI CTAG 3 cut(s) 188, 797, 809
GlaI GCGC 1 cut(s) 262
GluI GCNGC 3 cut(s) 147, 150, 774
GsuI CTGGAG 1 cut(s) 615
HaeII RGCGCY 1 cut(s) 264
HaeIII GGCC 1 cut(s) 497
HapII CCGG 1 cut(s) 342
HgaI GACGC 2 cut(s) 346, 665
HhaI GCGC 1 cut(s) 263
Hin1I GRCGYC 2 cut(s) 261, 338
Hin1II CATG 4 cut(s) 156, 553, 642, 673
Hin6I GCGC 1 cut(s) 261
HinP1I GCGC 1 cut(s) 261
HindIII AAGCTT 1 cut(s) 815
HinfI GANTC 1 cut(s) 743
HpaII CCGG 1 cut(s) 342
HphI GGTGA 1 cut(s) 31
Hpy166II GTNNAC 4 cut(s) 22, 384, 415, 511
Hpy188I TCNGA 3 cut(s) 312, 421, 687
Hpy188III TCNNGA 3 cut(s) 128, 616, 747
Hpy8I GTNNAC 4 cut(s) 22, 384, 415, 511
Hpy99I CGWCG 1 cut(s) 659
HpyAV CCTTC 1 cut(s) 307
HpyCH4III ACNGT 3 cut(s) 14, 412, 781
HpyCH4IV ACGT 2 cut(s) 327, 439
HpyCH4V TGCA 7 cut(s) 149, 224, 278, 306, 407, 638, 791
HpyF10VI GCNNNNNNNGC 4 cut(s) 644, 648, 773, 818
HpyF3I CTNAG 1 cut(s) 26
HpySE526I ACGT 2 cut(s) 327, 439
Hsp92I GRCGYC 2 cut(s) 261, 338
Hsp92II CATG 4 cut(s) 156, 553, 642, 673
HspAI GCGC 1 cut(s) 261
KasI GGCGCC 1 cut(s) 260
Kzo9I GATC 4 cut(s) 124, 500, 622, 749
LmnI GCTCC 2 cut(s) 596, 701
Lsp1109I GCAGC 3 cut(s) 133, 161, 785
LweI GCATC 1 cut(s) 608
MaeI CTAG 3 cut(s) 188, 797, 809
MaeII ACGT 2 cut(s) 327, 439
MaeIII GTNAC 1 cut(s) 317
MalI GATC 4 cut(s) 126, 502, 624, 751
MboI GATC 4 cut(s) 124, 500, 622, 749
MboII GAAGA 6 cut(s) 43, 46, 343, 346, 368, 468
MflI RGATCY 2 cut(s) 622, 749
MluCI AATT 4 cut(s) 30, 91, 99, 134
Mly113I GGCGCC 1 cut(s) 261
MmeI TCCRAC 4 cut(s) 535, 599, 691, 777
MnlI CCTC 3 cut(s) 454, 587, 707
MseI TTAA 4 cut(s) 5, 369, 666, 783
MspI CCGG 1 cut(s) 342
MspR9I CCNGG 3 cut(s) 266, 342, 521
Mva1269I GAATGC 1 cut(s) 306
MvaI CCWGG 2 cut(s) 266, 521
MwoI GCNNNNNNNGC 4 cut(s) 644, 648, 773, 818
NarI GGCGCC 1 cut(s) 261
NciI CCSGG 1 cut(s) 342
NdeII GATC 4 cut(s) 124, 500, 622, 749
NlaIII CATG 4 cut(s) 156, 553, 642, 673
NlaIV GGNNCC 2 cut(s) 262, 718
NspI RCATGY 1 cut(s) 642
PaeI GCATGC 1 cut(s) 642
PctI GAATGC 1 cut(s) 306
PfeI GAWTC 1 cut(s) 743
PflMI CCANNNNNTGG 2 cut(s) 271, 800
PkrI GCNGC 3 cut(s) 148, 151, 775
PluTI GGCGCC 1 cut(s) 264
PpuMI RGGWCCY 1 cut(s) 716
Psp5II RGGWCCY 1 cut(s) 716
Psp6I CCWGG 2 cut(s) 264, 519
PspGI CCWGG 2 cut(s) 264, 519
PspN4I GGNNCC 2 cut(s) 262, 718
PspPI GGNCC 2 cut(s) 496, 716
PspPPI RGGWCCY 1 cut(s) 716
PstI CTGCAG 1 cut(s) 151
PstNI CAGNNNCTG 1 cut(s) 779
PsuI RGATCY 2 cut(s) 622, 749
RsaI GTAC 1 cut(s) 185
RsaNI GTAC 1 cut(s) 184
SaqAI TTAA 4 cut(s) 5, 369, 666, 783
SatI GCNGC 3 cut(s) 147, 150, 774
Sau3AI GATC 4 cut(s) 124, 500, 622, 749
Sau96I GGNCC 2 cut(s) 496, 716
ScrFI CCNGG 3 cut(s) 266, 342, 521
SfaNI GCATC 1 cut(s) 608
SfcI CTRYAG 1 cut(s) 147
SfoI GGCGCC 1 cut(s) 262
SinI GGWCC 1 cut(s) 716
SmlI CTYRAG 1 cut(s) 579
SmoI CTYRAG 1 cut(s) 579
SpeI ACTAGT 1 cut(s) 796
SphI GCATGC 1 cut(s) 642
Sse9I AATT 4 cut(s) 30, 91, 99, 134
SspDI GGCGCC 1 cut(s) 260
SspMI CTAG 3 cut(s) 188, 797, 809
StyD4I CCNGG 3 cut(s) 264, 340, 519
StyI CCWWGG 1 cut(s) 196
TaaI ACNGT 3 cut(s) 14, 412, 781
TaiI ACGT 2 cut(s) 330, 442
TaqI TCGA 3 cut(s) 9, 123, 654
TasI AATT 4 cut(s) 30, 91, 99, 134
TfiI GAWTC 1 cut(s) 743
Tru1I TTAA 4 cut(s) 5, 369, 666, 783
Tru9I TTAA 4 cut(s) 5, 369, 666, 783
TscAI CASTG 1 cut(s) 19
TseI GCWGC 3 cut(s) 146, 149, 773
TspDTI ATGAA 5 cut(s) 147, 149, 283, 575, 686
TspGWI ACGGA 1 cut(s) 755
TspRI CASTG 1 cut(s) 19
Van91I CCANNNNNTGG 2 cut(s) 271, 800
VpaK11BI GGWCC 1 cut(s) 716
XapI RAATTY 1 cut(s) 91
XceI RCATGY 1 cut(s) 642
XcmI CCANNNNNNNNNTGG 1 cut(s) 760
XmiI GTMKAC 1 cut(s) 383
XspI CTAG 3 cut(s) 188, 797, 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.