Regulatory subunit of the dimeric E1 enzyme. E1 activates RUB1 NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AbsI | CCTCGAGG | 1 cut(s) | 143 |
| AccB1I | GGYRCC | 1 cut(s) | 136 |
| AclWI | GGATC | 1 cut(s) | 157 |
| AcsI | RAATTY | 1 cut(s) | 107 |
| AfaI | GTAC | 2 cut(s) | 69, 156 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 146, 214 |
| AgsI | TTSAA | 2 cut(s) | 103, 174 |
| AluBI | AGCT | 1 cut(s) | 191 |
| AluI | AGCT | 1 cut(s) | 191 |
| Alw21I | GWGCWC | 1 cut(s) | 183 |
| Alw26I | GTCTC | 2 cut(s) | 8, 37 |
| AlwI | GGATC | 1 cut(s) | 157 |
| Ama87I | CYCGRG | 1 cut(s) | 143 |
| ApoI | RAATTY | 1 cut(s) | 107 |
| AsuHPI | GGTGA | 1 cut(s) | 143 |
| AvaI | CYCGRG | 1 cut(s) | 143 |
| BanI | GGYRCC | 1 cut(s) | 136 |
| Bbv12I | GWGCWC | 1 cut(s) | 183 |
| BbvCI | CCTCAGC | 1 cut(s) | 219 |
| BcoDI | GTCTC | 2 cut(s) | 8, 37 |
| BfaI | CTAG | 4 cut(s) | 60, 81, 113, 233 |
| BmeT110I | CYCGRG | 1 cut(s) | 143 |
| BmiI | GGNNCC | 1 cut(s) | 138 |
| Bpu10I | CCTNAGC | 1 cut(s) | 219 |
| BsaJI | CCNNGG | 1 cut(s) | 213 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 146, 214 |
| BseDI | CCNNGG | 1 cut(s) | 213 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 146, 214 |
| BseMII | CTCAG | 2 cut(s) | 168, 210 |
| BshNI | GGYRCC | 1 cut(s) | 136 |
| BsiHKAI | GWGCWC | 1 cut(s) | 183 |
| BsiHKCI | CYCGRG | 1 cut(s) | 143 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 146, 214 |
| BsmAI | GTCTC | 2 cut(s) | 8, 37 |
| BsoBI | CYCGRG | 1 cut(s) | 143 |
| Bsp1286I | GDGCHC | 1 cut(s) | 183 |
| Bsp143I | GATC | 1 cut(s) | 149 |
| BspCNI | CTCAG | 3 cut(s) | 4, 169, 211 |
| BspLI | GGNNCC | 1 cut(s) | 138 |
| BspPI | GGATC | 1 cut(s) | 157 |
| BspT107I | GGYRCC | 1 cut(s) | 136 |
| BssECI | CCNNGG | 1 cut(s) | 213 |
| BssMI | GATC | 1 cut(s) | 149 |
| BssT1I | CCWWGG | 1 cut(s) | 213 |
| Bst6I | CTCTTC | 1 cut(s) | 81 |
| BstDEI | CTNAG | 3 cut(s) | 12, 177, 219 |
| BstKTI | GATC | 1 cut(s) | 152 |
| BstMAI | GTCTC | 2 cut(s) | 8, 37 |
| BstMBI | GATC | 1 cut(s) | 149 |
| Csp6I | GTAC | 2 cut(s) | 68, 155 |
| CviJI | RGCY | 4 cut(s) | 191, 212, 218, 224 |
| CviKI_1 | RGCY | 4 cut(s) | 191, 212, 218, 224 |
| CviQI | GTAC | 2 cut(s) | 68, 155 |
| DdeI | CTNAG | 3 cut(s) | 12, 177, 219 |
| DpnI | GATC | 1 cut(s) | 151 |
| DpnII | GATC | 1 cut(s) | 149 |
| Eam1104I | CTCTTC | 1 cut(s) | 81 |
| EarI | CTCTTC | 1 cut(s) | 81 |
| Eco130I | CCWWGG | 1 cut(s) | 213 |
| Eco88I | CYCGRG | 1 cut(s) | 143 |
| EcoRI | GAATTC | 1 cut(s) | 107 |
| EcoT14I | CCWWGG | 1 cut(s) | 213 |
| ErhI | CCWWGG | 1 cut(s) | 213 |
| FaiI | YATR | 2 cut(s) | 164, 186 |
| FspBI | CTAG | 4 cut(s) | 60, 81, 113, 233 |
| HinfI | GANTC | 1 cut(s) | 255 |
| HphI | GGTGA | 1 cut(s) | 143 |
| Hpy166II | GTNNAC | 1 cut(s) | 68 |
| Hpy188I | TCNGA | 2 cut(s) | 13, 48 |
| Hpy8I | GTNNAC | 1 cut(s) | 68 |
| HpyAV | CCTTC | 1 cut(s) | 116 |
| HpyCH4V | TGCA | 1 cut(s) | 41 |
| HpyF3I | CTNAG | 3 cut(s) | 12, 177, 219 |
| Kzo9I | GATC | 1 cut(s) | 149 |
| LpnPI | CCDG | 1 cut(s) | 171 |
| MaeI | CTAG | 4 cut(s) | 60, 81, 113, 233 |
| MalI | GATC | 1 cut(s) | 151 |
| MboI | GATC | 1 cut(s) | 149 |
| MboII | GAAGA | 3 cut(s) | 98, 162, 250 |
| MhlI | GDGCHC | 1 cut(s) | 183 |
| MluCI | AATT | 1 cut(s) | 107 |
| MlyI | GAGTC | 1 cut(s) | 249 |
| MmeI | TCCRAC | 1 cut(s) | 98 |
| MnlI | CCTC | 4 cut(s) | 127, 139, 152, 214 |
| NdeII | GATC | 1 cut(s) | 149 |
| NlaIV | GGNNCC | 1 cut(s) | 138 |
| PaeR7I | CTCGAG | 1 cut(s) | 143 |
| PleI | GAGTC | 1 cut(s) | 249 |
| PpsI | GAGTC | 1 cut(s) | 249 |
| PspN4I | GGNNCC | 1 cut(s) | 138 |
| PspXI | VCTCGAGB | 1 cut(s) | 143 |
| RsaI | GTAC | 2 cut(s) | 69, 156 |
| RsaNI | GTAC | 2 cut(s) | 68, 155 |
| Sau3AI | GATC | 1 cut(s) | 149 |
| SchI | GAGTC | 1 cut(s) | 249 |
| SduI | GDGCHC | 1 cut(s) | 183 |
| SetI | ASST | 3 cut(s) | 127, 193, 206 |
| Sfr274I | CTCGAG | 1 cut(s) | 143 |
| SlaI | CTCGAG | 1 cut(s) | 143 |
| SmlI | CTYRAG | 1 cut(s) | 143 |
| SmoI | CTYRAG | 1 cut(s) | 143 |
| Sse9I | AATT | 1 cut(s) | 107 |
| SspMI | CTAG | 4 cut(s) | 60, 81, 113, 233 |
| StyI | CCWWGG | 1 cut(s) | 213 |
| TaqI | TCGA | 1 cut(s) | 144 |
| TasI | AATT | 1 cut(s) | 107 |
| TatI | WGTACW | 1 cut(s) | 67 |
| TspDTI | ATGAA | 1 cut(s) | 135 |
| XapI | RAATTY | 1 cut(s) | 107 |
| XhoI | CTCGAG | 1 cut(s) | 143 |
| XspI | CTAG | 4 cut(s) | 60, 81, 113, 233 |