Regulatory subunit of the dimeric E1 enzyme. E1 activates RUB1 NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 458 |
| AbsI | CCTCGAGG | 1 cut(s) | 254 |
| Acc36I | ACCTGC | 1 cut(s) | 510 |
| AccB1I | GGYRCC | 1 cut(s) | 247 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 651 |
| AclWI | GGATC | 1 cut(s) | 268 |
| AcsI | RAATTY | 1 cut(s) | 218 |
| AfaI | GTAC | 2 cut(s) | 119, 267 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 257, 325, 651 |
| AgsI | TTSAA | 2 cut(s) | 91, 285 |
| AluBI | AGCT | 3 cut(s) | 109, 127, 302 |
| AluI | AGCT | 3 cut(s) | 109, 127, 302 |
| Alw21I | GWGCWC | 1 cut(s) | 294 |
| Alw26I | GTCTC | 1 cut(s) | 467 |
| AlwI | GGATC | 1 cut(s) | 268 |
| Ama87I | CYCGRG | 1 cut(s) | 254 |
| AoxI | GGCC | 1 cut(s) | 101 |
| ApeKI | GCWGC | 2 cut(s) | 395, 449 |
| ApoI | RAATTY | 1 cut(s) | 218 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 69 |
| AsuHPI | GGTGA | 1 cut(s) | 254 |
| AvaI | CYCGRG | 1 cut(s) | 254 |
| BanI | GGYRCC | 1 cut(s) | 247 |
| BbsI | GAAGAC | 1 cut(s) | 367 |
| Bbv12I | GWGCWC | 1 cut(s) | 294 |
| BbvCI | CCTCAGC | 1 cut(s) | 330 |
| BbvI | GCAGC | 2 cut(s) | 382, 436 |
| BccI | CCATC | 1 cut(s) | 397 |
| BcoDI | GTCTC | 1 cut(s) | 467 |
| BfaI | CTAG | 2 cut(s) | 138, 224 |
| BfmI | CTRYAG | 3 cut(s) | 147, 166, 663 |
| BfuAI | ACCTGC | 1 cut(s) | 510 |
| BisI | GCNGC | 2 cut(s) | 396, 450 |
| BlsI | GCNGC | 2 cut(s) | 397, 451 |
| BmeT110I | CYCGRG | 1 cut(s) | 254 |
| BmiI | GGNNCC | 1 cut(s) | 249 |
| BmsI | GCATC | 2 cut(s) | 372, 576 |
| BoxI | GACNNNNGTC | 1 cut(s) | 630 |
| BpiI | GAAGAC | 1 cut(s) | 367 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 464, 496 |
| Bpu10I | CCTNAGC | 2 cut(s) | 105, 330 |
| BpuEI | CTTGAG | 1 cut(s) | 227 |
| BsaJI | CCNNGG | 3 cut(s) | 98, 324, 365 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 257, 325, 651 |
| BseDI | CCNNGG | 3 cut(s) | 98, 324, 365 |
| BseGI | GGATG | 3 cut(s) | 82, 147, 660 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 257, 325, 651 |
| BseMII | CTCAG | 7 cut(s) | 96, 279, 321, 413, 435, 593, 618 |
| BseRI | GAGGAG | 1 cut(s) | 472 |
| BseXI | GCAGC | 2 cut(s) | 382, 436 |
| BsgI | GTGCAG | 1 cut(s) | 53 |
| BshFI | GGCC | 1 cut(s) | 103 |
| BshNI | GGYRCC | 1 cut(s) | 247 |
| BsiHKAI | GWGCWC | 1 cut(s) | 294 |
| BsiHKCI | CYCGRG | 1 cut(s) | 254 |
| BsiSI | CCGG | 1 cut(s) | 498 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 257, 325, 651 |
| BsmAI | GTCTC | 1 cut(s) | 467 |
| BsnI | GGCC | 1 cut(s) | 103 |
| BsoBI | CYCGRG | 1 cut(s) | 254 |
| Bsp1286I | GDGCHC | 1 cut(s) | 294 |
| Bsp143I | GATC | 1 cut(s) | 260 |
| Bsp19I | CCATGG | 1 cut(s) | 98 |
| BspANI | GGCC | 1 cut(s) | 103 |
| BspCNI | CTCAG | 7 cut(s) | 97, 280, 322, 412, 436, 592, 617 |
| BspLI | GGNNCC | 1 cut(s) | 249 |
| BspMAI | CTGCAG | 1 cut(s) | 151 |
| BspMI | ACCTGC | 1 cut(s) | 510 |
| BspPI | GGATC | 1 cut(s) | 268 |
| BspT107I | GGYRCC | 1 cut(s) | 247 |
| BssECI | CCNNGG | 3 cut(s) | 98, 324, 365 |
| BssMI | GATC | 1 cut(s) | 260 |
| BssT1I | CCWWGG | 3 cut(s) | 98, 324, 365 |
| Bst4CI | ACNGT | 4 cut(s) | 46, 170, 373, 427 |
| Bst6I | CTCTTC | 2 cut(s) | 581, 606 |
| BstC8I | GCNNGC | 2 cut(s) | 565, 679 |
| BstDEI | CTNAG | 8 cut(s) | 105, 110, 288, 330, 399, 444, 579, 604 |
| BstDSI | CCRYGG | 1 cut(s) | 98 |
| BstF5I | GGATG | 3 cut(s) | 82, 147, 660 |
| BstKTI | GATC | 1 cut(s) | 263 |
| BstMAI | GTCTC | 1 cut(s) | 467 |
| BstMBI | GATC | 1 cut(s) | 260 |
| BstNSI | RCATGY | 1 cut(s) | 415 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 630 |
| BstSFI | CTRYAG | 3 cut(s) | 147, 166, 663 |
| BstV1I | GCAGC | 2 cut(s) | 382, 436 |
| BstV2I | GAAGAC | 1 cut(s) | 367 |
| BsuRI | GGCC | 1 cut(s) | 103 |
| BtgI | CCRYGG | 1 cut(s) | 98 |
| BtsCI | GGATG | 3 cut(s) | 82, 147, 660 |
| BtsIMutI | CAGTG | 3 cut(s) | 175, 587, 612 |
| BveI | ACCTGC | 1 cut(s) | 510 |
| Cac8I | GCNNGC | 2 cut(s) | 565, 679 |
| Csp6I | GTAC | 2 cut(s) | 118, 266 |
| CviAII | CATG | 2 cut(s) | 99, 412 |
| CviQI | GTAC | 2 cut(s) | 118, 266 |
| DdeI | CTNAG | 8 cut(s) | 105, 110, 288, 330, 399, 444, 579, 604 |
| DpnI | GATC | 1 cut(s) | 262 |
| DpnII | GATC | 1 cut(s) | 260 |
| DraI | TTTAAA | 1 cut(s) | 192 |
| Eam1104I | CTCTTC | 2 cut(s) | 581, 606 |
| EarI | CTCTTC | 2 cut(s) | 581, 606 |
| Eco130I | CCWWGG | 3 cut(s) | 98, 324, 365 |
| Eco88I | CYCGRG | 1 cut(s) | 254 |
| EcoRI | GAATTC | 1 cut(s) | 218 |
| EcoT14I | CCWWGG | 3 cut(s) | 98, 324, 365 |
| ErhI | CCWWGG | 3 cut(s) | 98, 324, 365 |
| FaeI | CATG | 2 cut(s) | 102, 415 |
| FatI | CATG | 2 cut(s) | 98, 411 |
| Fnu4HI | GCNGC | 2 cut(s) | 396, 450 |
| FokI | GGATG | 3 cut(s) | 89, 154, 667 |
| Fsp4HI | GCNGC | 2 cut(s) | 396, 450 |
| FspBI | CTAG | 2 cut(s) | 138, 224 |
| GluI | GCNGC | 2 cut(s) | 396, 450 |
| HaeIII | GGCC | 1 cut(s) | 103 |
| HapII | CCGG | 1 cut(s) | 498 |
| Hin1II | CATG | 2 cut(s) | 102, 415 |
| HinfI | GANTC | 1 cut(s) | 53 |
| HpaII | CCGG | 1 cut(s) | 498 |
| HphI | GGTGA | 1 cut(s) | 254 |
| Hpy166II | GTNNAC | 1 cut(s) | 625 |
| Hpy188I | TCNGA | 3 cut(s) | 402, 445, 636 |
| Hpy188III | TCNNGA | 4 cut(s) | 74, 379, 430, 504 |
| Hpy8I | GTNNAC | 1 cut(s) | 625 |
| HpyAV | CCTTC | 5 cut(s) | 16, 92, 108, 227, 688 |
| HpyCH4III | ACNGT | 4 cut(s) | 46, 170, 373, 427 |
| HpyCH4V | TGCA | 5 cut(s) | 34, 149, 415, 563, 677 |
| HpyF3I | CTNAG | 8 cut(s) | 105, 110, 288, 330, 399, 444, 579, 604 |
| Hsp92II | CATG | 2 cut(s) | 102, 415 |
| Kzo9I | GATC | 1 cut(s) | 260 |
| LpnPI | CCDG | 7 cut(s) | 13, 59, 75, 117, 282, 505, 511 |
| Lsp1109I | GCAGC | 2 cut(s) | 382, 436 |
| LweI | GCATC | 2 cut(s) | 372, 576 |
| MaeI | CTAG | 2 cut(s) | 138, 224 |
| MalI | GATC | 1 cut(s) | 262 |
| MboI | GATC | 1 cut(s) | 260 |
| MboII | GAAGA | 4 cut(s) | 273, 367, 598, 623 |
| MhlI | GDGCHC | 1 cut(s) | 294 |
| MluCI | AATT | 4 cut(s) | 212, 218, 493, 529 |
| MlyI | GAGTC | 1 cut(s) | 62 |
| MroXI | GAANNNNTTC | 1 cut(s) | 69 |
| MseI | TTAA | 2 cut(s) | 191, 467 |
| MspI | CCGG | 1 cut(s) | 498 |
| NcoI | CCATGG | 1 cut(s) | 98 |
| NdeII | GATC | 1 cut(s) | 260 |
| NlaIII | CATG | 2 cut(s) | 102, 415 |
| NlaIV | GGNNCC | 1 cut(s) | 249 |
| NspI | RCATGY | 1 cut(s) | 415 |
| PaeR7I | CTCGAG | 1 cut(s) | 254 |
| PdmI | GAANNNNTTC | 1 cut(s) | 69 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 651 |
| PkrI | GCNGC | 2 cut(s) | 397, 451 |
| PleI | GAGTC | 1 cut(s) | 61 |
| PpsI | GAGTC | 1 cut(s) | 61 |
| PshAI | GACNNNNGTC | 1 cut(s) | 630 |
| PsiI | TTATAA | 1 cut(s) | 458 |
| PspN4I | GGNNCC | 1 cut(s) | 249 |
| PspXI | VCTCGAGB | 1 cut(s) | 254 |
| PstI | CTGCAG | 1 cut(s) | 151 |
| RsaI | GTAC | 2 cut(s) | 119, 267 |
| RsaNI | GTAC | 2 cut(s) | 118, 266 |
| SaqAI | TTAA | 2 cut(s) | 191, 467 |
| SatI | GCNGC | 2 cut(s) | 396, 450 |
| Sau3AI | GATC | 1 cut(s) | 260 |
| SchI | GAGTC | 1 cut(s) | 62 |
| SduI | GDGCHC | 1 cut(s) | 294 |
| SfaNI | GCATC | 2 cut(s) | 372, 576 |
| SfcI | CTRYAG | 3 cut(s) | 147, 166, 663 |
| Sfr274I | CTCGAG | 1 cut(s) | 254 |
| SlaI | CTCGAG | 1 cut(s) | 254 |
| SmlI | CTYRAG | 2 cut(s) | 206, 254 |
| SmoI | CTYRAG | 2 cut(s) | 206, 254 |
| Sse9I | AATT | 4 cut(s) | 212, 218, 493, 529 |
| SspMI | CTAG | 2 cut(s) | 138, 224 |
| StyI | CCWWGG | 3 cut(s) | 98, 324, 365 |
| TaaI | ACNGT | 4 cut(s) | 46, 170, 373, 427 |
| TaqI | TCGA | 2 cut(s) | 255, 505 |
| TasI | AATT | 4 cut(s) | 212, 218, 493, 529 |
| Tru1I | TTAA | 2 cut(s) | 191, 467 |
| Tru9I | TTAA | 2 cut(s) | 191, 467 |
| TscAI | CASTG | 3 cut(s) | 175, 587, 612 |
| TseI | GCWGC | 2 cut(s) | 395, 449 |
| TspDTI | ATGAA | 1 cut(s) | 246 |
| TspGWI | ACGGA | 1 cut(s) | 620 |
| TspRI | CASTG | 3 cut(s) | 175, 587, 612 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 651 |
| XapI | RAATTY | 1 cut(s) | 218 |
| XceI | RCATGY | 1 cut(s) | 415 |
| XhoI | CTCGAG | 1 cut(s) | 254 |
| XmnI | GAANNNNTTC | 1 cut(s) | 69 |
| XspI | CTAG | 2 cut(s) | 138, 224 |