Regulatory subunit of the dimeric E1 enzyme. E1 activates RUB1 NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 121 |
| AccI | GTMKAC | 1 cut(s) | 171 |
| AclWI | GGATC | 1 cut(s) | 142 |
| AcsI | RAATTY | 2 cut(s) | 92, 183 |
| AfaI | GTAC | 2 cut(s) | 15, 58 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 131 |
| AgsI | TTSAA | 2 cut(s) | 43, 159 |
| AlwI | GGATC | 1 cut(s) | 142 |
| AoxI | GGCC | 1 cut(s) | 76 |
| ApoI | RAATTY | 2 cut(s) | 92, 183 |
| AspS9I | GGNCC | 1 cut(s) | 77 |
| AsuHPI | GGTGA | 2 cut(s) | 43, 128 |
| BanI | GGYRCC | 1 cut(s) | 121 |
| BclI | TGATCA | 1 cut(s) | 33 |
| BfaI | CTAG | 1 cut(s) | 98 |
| BmgT120I | GGNCC | 1 cut(s) | 77 |
| BmiI | GGNNCC | 2 cut(s) | 79, 123 |
| BpuEI | CTTGAG | 1 cut(s) | 149 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 131 |
| BseGI | GGATG | 1 cut(s) | 174 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 131 |
| BseMII | CTCAG | 1 cut(s) | 153 |
| BshFI | GGCC | 1 cut(s) | 78 |
| BshNI | GGYRCC | 1 cut(s) | 121 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 131 |
| BsnI | GGCC | 1 cut(s) | 78 |
| Bsp143I | GATC | 2 cut(s) | 33, 134 |
| BspANI | GGCC | 1 cut(s) | 78 |
| BspCNI | CTCAG | 1 cut(s) | 154 |
| BspLI | GGNNCC | 2 cut(s) | 79, 123 |
| BspPI | GGATC | 1 cut(s) | 142 |
| BspT107I | GGYRCC | 1 cut(s) | 121 |
| BssMI | GATC | 2 cut(s) | 33, 134 |
| BstDEI | CTNAG | 1 cut(s) | 162 |
| BstF5I | GGATG | 1 cut(s) | 174 |
| BstKTI | GATC | 2 cut(s) | 36, 137 |
| BstMBI | GATC | 2 cut(s) | 33, 134 |
| BsuRI | GGCC | 1 cut(s) | 78 |
| BtsCI | GGATG | 1 cut(s) | 174 |
| Cfr13I | GGNCC | 1 cut(s) | 77 |
| Csp6I | GTAC | 2 cut(s) | 14, 57 |
| CviAII | CATG | 1 cut(s) | 189 |
| CviJI | RGCY | 1 cut(s) | 78 |
| CviKI_1 | RGCY | 1 cut(s) | 78 |
| CviQI | GTAC | 2 cut(s) | 14, 57 |
| DdeI | CTNAG | 1 cut(s) | 162 |
| DpnI | GATC | 2 cut(s) | 35, 136 |
| DpnII | GATC | 2 cut(s) | 33, 134 |
| EcoRI | GAATTC | 1 cut(s) | 92 |
| FaeI | CATG | 1 cut(s) | 192 |
| FaiI | YATR | 4 cut(s) | 22, 87, 149, 190 |
| FatI | CATG | 1 cut(s) | 188 |
| FbaI | TGATCA | 1 cut(s) | 33 |
| FblI | GTMKAC | 1 cut(s) | 171 |
| FokI | GGATG | 1 cut(s) | 161 |
| FspBI | CTAG | 1 cut(s) | 98 |
| HaeIII | GGCC | 1 cut(s) | 78 |
| Hin1II | CATG | 1 cut(s) | 192 |
| HincII | GTYRAC | 1 cut(s) | 172 |
| HindII | GTYRAC | 1 cut(s) | 172 |
| HinfI | GANTC | 2 cut(s) | 26, 69 |
| HphI | GGTGA | 2 cut(s) | 43, 128 |
| Hpy166II | GTNNAC | 1 cut(s) | 172 |
| Hpy8I | GTNNAC | 1 cut(s) | 172 |
| HpyAV | CCTTC | 1 cut(s) | 101 |
| HpyF3I | CTNAG | 1 cut(s) | 162 |
| Hsp92II | CATG | 1 cut(s) | 192 |
| Ksp22I | TGATCA | 1 cut(s) | 33 |
| Kzo9I | GATC | 2 cut(s) | 33, 134 |
| LpnPI | CCDG | 2 cut(s) | 156, 193 |
| MaeI | CTAG | 1 cut(s) | 98 |
| MalI | GATC | 2 cut(s) | 35, 136 |
| MboI | GATC | 2 cut(s) | 33, 134 |
| MboII | GAAGA | 2 cut(s) | 101, 147 |
| MluCI | AATT | 2 cut(s) | 92, 183 |
| MnlI | CCTC | 3 cut(s) | 41, 112, 124 |
| MseI | TTAA | 1 cut(s) | 208 |
| NdeII | GATC | 2 cut(s) | 33, 134 |
| NlaIII | CATG | 1 cut(s) | 192 |
| NlaIV | GGNNCC | 2 cut(s) | 79, 123 |
| PfeI | GAWTC | 2 cut(s) | 26, 69 |
| PspN4I | GGNNCC | 2 cut(s) | 79, 123 |
| PspPI | GGNCC | 1 cut(s) | 77 |
| RsaI | GTAC | 2 cut(s) | 15, 58 |
| RsaNI | GTAC | 2 cut(s) | 14, 57 |
| SalI | GTCGAC | 1 cut(s) | 170 |
| SaqAI | TTAA | 1 cut(s) | 208 |
| Sau3AI | GATC | 2 cut(s) | 33, 134 |
| Sau96I | GGNCC | 1 cut(s) | 77 |
| SetI | ASST | 3 cut(s) | 9, 52, 112 |
| SgeI | CNNG | 7 cut(s) | 49, 94, 110, 140, 155, 192, 201 |
| SmlI | CTYRAG | 1 cut(s) | 128 |
| SmoI | CTYRAG | 1 cut(s) | 128 |
| Sse9I | AATT | 2 cut(s) | 92, 183 |
| SspI | AATATT | 1 cut(s) | 64 |
| SspMI | CTAG | 1 cut(s) | 98 |
| TaqI | TCGA | 1 cut(s) | 171 |
| TasI | AATT | 2 cut(s) | 92, 183 |
| TfiI | GAWTC | 2 cut(s) | 26, 69 |
| Tru1I | TTAA | 1 cut(s) | 208 |
| Tru9I | TTAA | 1 cut(s) | 208 |
| TspDTI | ATGAA | 3 cut(s) | 102, 120, 177 |
| XapI | RAATTY | 2 cut(s) | 92, 183 |
| XmiI | GTMKAC | 1 cut(s) | 171 |
| XspI | CTAG | 1 cut(s) | 98 |