Regulatory subunit of the dimeric E1 enzyme. E1 activates RUB1 NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AbsI | CCTCGAGG | 1 cut(s) | 125 |
| AccB1I | GGYRCC | 1 cut(s) | 118 |
| AciI | CCGC | 2 cut(s) | 8, 193 |
| AclWI | GGATC | 2 cut(s) | 139, 211 |
| AcsI | RAATTY | 1 cut(s) | 89 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 128 |
| AflIII | ACRYGT | 1 cut(s) | 399 |
| AgsI | TTSAA | 5 cut(s) | 38, 156, 397, 410, 423 |
| AluBI | AGCT | 2 cut(s) | 204, 308 |
| AluI | AGCT | 2 cut(s) | 204, 308 |
| Alw26I | GTCTC | 2 cut(s) | 183, 229 |
| AlwI | GGATC | 2 cut(s) | 139, 211 |
| Ama87I | CYCGRG | 1 cut(s) | 125 |
| ApoI | RAATTY | 1 cut(s) | 89 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 389 |
| AsuHPI | GGTGA | 1 cut(s) | 125 |
| AsuII | TTCGAA | 1 cut(s) | 350 |
| AvaI | CYCGRG | 1 cut(s) | 125 |
| BanI | GGYRCC | 1 cut(s) | 118 |
| BbsI | GAAGAC | 3 cut(s) | 243, 251, 259 |
| BccI | CCATC | 4 cut(s) | 239, 300, 385, 437 |
| BcoDI | GTCTC | 2 cut(s) | 183, 229 |
| BfaI | CTAG | 1 cut(s) | 95 |
| BglII | AGATCT | 1 cut(s) | 73 |
| BisI | GCNGC | 1 cut(s) | 9 |
| BlsI | GCNGC | 1 cut(s) | 10 |
| BmeT110I | CYCGRG | 1 cut(s) | 125 |
| BmiI | GGNNCC | 1 cut(s) | 120 |
| BmrI | ACTGGG | 1 cut(s) | 462 |
| BmuI | ACTGGG | 1 cut(s) | 462 |
| BpiI | GAAGAC | 3 cut(s) | 243, 251, 259 |
| Bpu14I | TTCGAA | 1 cut(s) | 350 |
| Bsa29I | ATCGAT | 1 cut(s) | 441 |
| BsaI | GGTCTC | 1 cut(s) | 183 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 128 |
| Bse1I | ACTGG | 2 cut(s) | 55, 457 |
| BseCI | ATCGAT | 1 cut(s) | 441 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 128 |
| BseMII | CTCAG | 2 cut(s) | 150, 184 |
| BseNI | ACTGG | 2 cut(s) | 55, 457 |
| BseRI | GAGGAG | 5 cut(s) | 217, 273, 317, 329, 354 |
| BshNI | GGYRCC | 1 cut(s) | 118 |
| BshVI | ATCGAT | 1 cut(s) | 441 |
| BsiHKCI | CYCGRG | 1 cut(s) | 125 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 128 |
| BsmAI | GTCTC | 2 cut(s) | 183, 229 |
| Bso31I | GGTCTC | 1 cut(s) | 183 |
| BsoBI | CYCGRG | 1 cut(s) | 125 |
| Bsp119I | TTCGAA | 1 cut(s) | 350 |
| Bsp143I | GATC | 4 cut(s) | 73, 131, 216, 299 |
| BspACI | CCGC | 2 cut(s) | 8, 193 |
| BspCNI | CTCAG | 2 cut(s) | 151, 183 |
| BspDI | ATCGAT | 1 cut(s) | 441 |
| BspLI | GGNNCC | 1 cut(s) | 120 |
| BspPI | GGATC | 2 cut(s) | 139, 211 |
| BspQI | GCTCTTC | 2 cut(s) | 277, 321 |
| BspT104I | TTCGAA | 1 cut(s) | 350 |
| BspT107I | GGYRCC | 1 cut(s) | 118 |
| BspTNI | GGTCTC | 1 cut(s) | 183 |
| BsrI | ACTGG | 2 cut(s) | 55, 457 |
| BssMI | GATC | 4 cut(s) | 73, 131, 216, 299 |
| Bst6I | CTCTTC | 2 cut(s) | 277, 321 |
| BstBI | TTCGAA | 1 cut(s) | 350 |
| BstDEI | CTNAG | 2 cut(s) | 159, 170 |
| BstKTI | GATC | 4 cut(s) | 76, 134, 219, 302 |
| BstMAI | GTCTC | 2 cut(s) | 183, 229 |
| BstMBI | GATC | 4 cut(s) | 73, 131, 216, 299 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 201 |
| BstV2I | GAAGAC | 3 cut(s) | 243, 251, 259 |
| BstX2I | RGATCY | 1 cut(s) | 73 |
| BstYI | RGATCY | 1 cut(s) | 73 |
| Bsu15I | ATCGAT | 1 cut(s) | 441 |
| BsuTUI | ATCGAT | 1 cut(s) | 441 |
| ClaI | ATCGAT | 1 cut(s) | 441 |
| CviAII | CATG | 1 cut(s) | 12 |
| CviJI | RGCY | 6 cut(s) | 174, 204, 264, 308, 356, 426 |
| CviKI_1 | RGCY | 6 cut(s) | 174, 204, 264, 308, 356, 426 |
| DdeI | CTNAG | 2 cut(s) | 159, 170 |
| DpnI | GATC | 4 cut(s) | 75, 133, 218, 301 |
| DpnII | GATC | 4 cut(s) | 73, 131, 216, 299 |
| Eam1104I | CTCTTC | 2 cut(s) | 277, 321 |
| EarI | CTCTTC | 2 cut(s) | 277, 321 |
| Eco31I | GGTCTC | 1 cut(s) | 183 |
| Eco88I | CYCGRG | 1 cut(s) | 125 |
| EcoRI | GAATTC | 1 cut(s) | 89 |
| FaeI | CATG | 1 cut(s) | 15 |
| FaiI | YATR | 4 cut(s) | 13, 57, 66, 146 |
| FatI | CATG | 1 cut(s) | 11 |
| Fnu4HI | GCNGC | 1 cut(s) | 9 |
| Fsp4HI | GCNGC | 1 cut(s) | 9 |
| FspBI | CTAG | 1 cut(s) | 95 |
| GluI | GCNGC | 1 cut(s) | 9 |
| Hin1II | CATG | 1 cut(s) | 15 |
| HinfI | GANTC | 9 cut(s) | 38, 166, 239, 247, 255, 347, 385, 416, 438 |
| HphI | GGTGA | 1 cut(s) | 125 |
| Hpy188I | TCNGA | 2 cut(s) | 304, 437 |
| Hpy188III | TCNNGA | 1 cut(s) | 178 |
| HpyAV | CCTTC | 3 cut(s) | 98, 254, 346 |
| HpyCH4IV | ACGT | 1 cut(s) | 401 |
| HpyCH4V | TGCA | 1 cut(s) | 64 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 201 |
| HpyF3I | CTNAG | 2 cut(s) | 159, 170 |
| HpySE526I | ACGT | 1 cut(s) | 401 |
| Hsp92II | CATG | 1 cut(s) | 15 |
| Kzo9I | GATC | 4 cut(s) | 73, 131, 216, 299 |
| LguI | GCTCTTC | 2 cut(s) | 277, 321 |
| LpnPI | CCDG | 3 cut(s) | 36, 153, 438 |
| MaeI | CTAG | 1 cut(s) | 95 |
| MaeII | ACGT | 1 cut(s) | 401 |
| MalI | GATC | 4 cut(s) | 75, 133, 218, 301 |
| MboI | GATC | 4 cut(s) | 73, 131, 216, 299 |
| MflI | RGATCY | 1 cut(s) | 73 |
| MluCI | AATT | 3 cut(s) | 68, 89, 389 |
| MlyI | GAGTC | 3 cut(s) | 233, 241, 249 |
| MroXI | GAANNNNTTC | 1 cut(s) | 389 |
| MseI | TTAA | 1 cut(s) | 474 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 201 |
| NdeII | GATC | 4 cut(s) | 73, 131, 216, 299 |
| NlaIII | CATG | 1 cut(s) | 15 |
| NlaIV | GGNNCC | 1 cut(s) | 120 |
| NspV | TTCGAA | 1 cut(s) | 350 |
| PaeR7I | CTCGAG | 1 cut(s) | 125 |
| PciSI | GCTCTTC | 2 cut(s) | 277, 321 |
| PdmI | GAANNNNTTC | 1 cut(s) | 389 |
| PfeI | GAWTC | 6 cut(s) | 38, 166, 347, 385, 416, 438 |
| PkrI | GCNGC | 1 cut(s) | 10 |
| PleI | GAGTC | 3 cut(s) | 233, 241, 249 |
| PpsI | GAGTC | 3 cut(s) | 233, 241, 249 |
| PspN4I | GGNNCC | 1 cut(s) | 120 |
| PspXI | VCTCGAGB | 1 cut(s) | 125 |
| PsuI | RGATCY | 1 cut(s) | 73 |
| SapI | GCTCTTC | 2 cut(s) | 277, 321 |
| SaqAI | TTAA | 1 cut(s) | 474 |
| SatI | GCNGC | 1 cut(s) | 9 |
| Sau3AI | GATC | 4 cut(s) | 73, 131, 216, 299 |
| SchI | GAGTC | 3 cut(s) | 233, 241, 249 |
| SetI | ASST | 5 cut(s) | 109, 206, 212, 310, 404 |
| Sfr274I | CTCGAG | 1 cut(s) | 125 |
| SfuI | TTCGAA | 1 cut(s) | 350 |
| SlaI | CTCGAG | 1 cut(s) | 125 |
| SmlI | CTYRAG | 1 cut(s) | 125 |
| SmoI | CTYRAG | 1 cut(s) | 125 |
| Sse9I | AATT | 3 cut(s) | 68, 89, 389 |
| SsiI | CCGC | 2 cut(s) | 8, 193 |
| SspMI | CTAG | 1 cut(s) | 95 |
| TaiI | ACGT | 1 cut(s) | 404 |
| TasI | AATT | 3 cut(s) | 68, 89, 389 |
| TauI | GCSGC | 1 cut(s) | 11 |
| TfiI | GAWTC | 6 cut(s) | 38, 166, 347, 385, 416, 438 |
| Tru1I | TTAA | 1 cut(s) | 474 |
| Tru9I | TTAA | 1 cut(s) | 474 |
| TspDTI | ATGAA | 1 cut(s) | 117 |
| XapI | RAATTY | 1 cut(s) | 89 |
| XhoI | CTCGAG | 1 cut(s) | 125 |
| XmnI | GAANNNNTTC | 1 cut(s) | 389 |
| XspI | CTAG | 1 cut(s) | 95 |