Regulatory subunit of the dimeric E1 enzyme. E1 activates RUB1 NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 118 |
| AccI | GTMKAC | 1 cut(s) | 168 |
| AciI | CCGC | 1 cut(s) | 8 |
| AclWI | GGATC | 1 cut(s) | 139 |
| AcsI | RAATTY | 3 cut(s) | 38, 89, 180 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 128, 256 |
| AgsI | TTSAA | 3 cut(s) | 16, 38, 156 |
| AluBI | AGCT | 1 cut(s) | 233 |
| AluI | AGCT | 1 cut(s) | 233 |
| AlwI | GGATC | 1 cut(s) | 139 |
| ApoI | RAATTY | 3 cut(s) | 38, 89, 180 |
| AsuHPI | GGTGA | 1 cut(s) | 125 |
| BanI | GGYRCC | 1 cut(s) | 118 |
| BbvCI | CCTCAGC | 1 cut(s) | 261 |
| BfaI | CTAG | 2 cut(s) | 95, 275 |
| BglII | AGATCT | 1 cut(s) | 73 |
| BisI | GCNGC | 1 cut(s) | 9 |
| BlsI | GCNGC | 1 cut(s) | 10 |
| BmiI | GGNNCC | 1 cut(s) | 120 |
| Bpu10I | CCTNAGC | 1 cut(s) | 261 |
| BpuEI | CTTGAG | 1 cut(s) | 110 |
| BsaJI | CCNNGG | 1 cut(s) | 255 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 128, 256 |
| Bse1I | ACTGG | 1 cut(s) | 55 |
| BseDI | CCNNGG | 1 cut(s) | 255 |
| BseGI | GGATG | 1 cut(s) | 171 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 128, 256 |
| BseMII | CTCAG | 2 cut(s) | 150, 252 |
| BseNI | ACTGG | 1 cut(s) | 55 |
| BshNI | GGYRCC | 1 cut(s) | 118 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 128, 256 |
| Bsp143I | GATC | 2 cut(s) | 73, 131 |
| BspACI | CCGC | 1 cut(s) | 8 |
| BspCNI | CTCAG | 2 cut(s) | 151, 253 |
| BspLI | GGNNCC | 1 cut(s) | 120 |
| BspPI | GGATC | 1 cut(s) | 139 |
| BspT107I | GGYRCC | 1 cut(s) | 118 |
| BsrI | ACTGG | 1 cut(s) | 55 |
| BssECI | CCNNGG | 1 cut(s) | 255 |
| BssMI | GATC | 2 cut(s) | 73, 131 |
| BssT1I | CCWWGG | 1 cut(s) | 255 |
| BstC8I | GCNNGC | 1 cut(s) | 221 |
| BstDEI | CTNAG | 2 cut(s) | 159, 261 |
| BstF5I | GGATG | 1 cut(s) | 171 |
| BstKTI | GATC | 2 cut(s) | 76, 134 |
| BstMBI | GATC | 2 cut(s) | 73, 131 |
| BstX2I | RGATCY | 1 cut(s) | 73 |
| BstYI | RGATCY | 1 cut(s) | 73 |
| BtsCI | GGATG | 1 cut(s) | 171 |
| Cac8I | GCNNGC | 1 cut(s) | 221 |
| CviAII | CATG | 1 cut(s) | 186 |
| CviJI | RGCY | 4 cut(s) | 233, 254, 260, 266 |
| CviKI_1 | RGCY | 4 cut(s) | 233, 254, 260, 266 |
| DdeI | CTNAG | 2 cut(s) | 159, 261 |
| DpnI | GATC | 2 cut(s) | 75, 133 |
| DpnII | GATC | 2 cut(s) | 73, 131 |
| Eco130I | CCWWGG | 1 cut(s) | 255 |
| EcoRI | GAATTC | 1 cut(s) | 89 |
| EcoT14I | CCWWGG | 1 cut(s) | 255 |
| ErhI | CCWWGG | 1 cut(s) | 255 |
| FaeI | CATG | 1 cut(s) | 189 |
| FaiI | YATR | 5 cut(s) | 57, 66, 146, 187, 228 |
| FatI | CATG | 1 cut(s) | 185 |
| FblI | GTMKAC | 1 cut(s) | 168 |
| Fnu4HI | GCNGC | 1 cut(s) | 9 |
| FokI | GGATG | 1 cut(s) | 158 |
| Fsp4HI | GCNGC | 1 cut(s) | 9 |
| FspBI | CTAG | 2 cut(s) | 95, 275 |
| GluI | GCNGC | 1 cut(s) | 9 |
| Hin1II | CATG | 1 cut(s) | 189 |
| HincII | GTYRAC | 1 cut(s) | 169 |
| HindII | GTYRAC | 1 cut(s) | 169 |
| HinfI | GANTC | 1 cut(s) | 297 |
| HphI | GGTGA | 1 cut(s) | 125 |
| Hpy166II | GTNNAC | 1 cut(s) | 169 |
| Hpy8I | GTNNAC | 1 cut(s) | 169 |
| HpyAV | CCTTC | 1 cut(s) | 98 |
| HpyCH4V | TGCA | 2 cut(s) | 64, 219 |
| HpyF3I | CTNAG | 2 cut(s) | 159, 261 |
| Hsp92II | CATG | 1 cut(s) | 189 |
| Kzo9I | GATC | 2 cut(s) | 73, 131 |
| LpnPI | CCDG | 4 cut(s) | 36, 153, 190, 205 |
| MaeI | CTAG | 2 cut(s) | 95, 275 |
| MalI | GATC | 2 cut(s) | 75, 133 |
| MboI | GATC | 2 cut(s) | 73, 131 |
| MboII | GAAGA | 3 cut(s) | 28, 144, 292 |
| MflI | RGATCY | 1 cut(s) | 73 |
| MluCI | AATT | 4 cut(s) | 38, 68, 89, 180 |
| MlyI | GAGTC | 1 cut(s) | 291 |
| MnlI | CCTC | 3 cut(s) | 109, 134, 256 |
| NdeII | GATC | 2 cut(s) | 73, 131 |
| NlaIII | CATG | 1 cut(s) | 189 |
| NlaIV | GGNNCC | 1 cut(s) | 120 |
| PkrI | GCNGC | 1 cut(s) | 10 |
| PleI | GAGTC | 1 cut(s) | 291 |
| PpsI | GAGTC | 1 cut(s) | 291 |
| PspN4I | GGNNCC | 1 cut(s) | 120 |
| PsuI | RGATCY | 1 cut(s) | 73 |
| SalI | GTCGAC | 1 cut(s) | 167 |
| SatI | GCNGC | 1 cut(s) | 9 |
| Sau3AI | GATC | 2 cut(s) | 73, 131 |
| SchI | GAGTC | 1 cut(s) | 291 |
| SetI | ASST | 3 cut(s) | 109, 235, 248 |
| SmlI | CTYRAG | 1 cut(s) | 125 |
| SmoI | CTYRAG | 1 cut(s) | 125 |
| Sse9I | AATT | 4 cut(s) | 38, 68, 89, 180 |
| SsiI | CCGC | 1 cut(s) | 8 |
| SspMI | CTAG | 2 cut(s) | 95, 275 |
| StyI | CCWWGG | 1 cut(s) | 255 |
| TaqI | TCGA | 1 cut(s) | 168 |
| TasI | AATT | 4 cut(s) | 38, 68, 89, 180 |
| TauI | GCSGC | 1 cut(s) | 11 |
| TspDTI | ATGAA | 2 cut(s) | 117, 174 |
| XapI | RAATTY | 3 cut(s) | 38, 89, 180 |
| XmiI | GTMKAC | 1 cut(s) | 168 |
| XspI | CTAG | 2 cut(s) | 95, 275 |