Regulatory subunit of the dimeric E1 enzyme. E1 activates RUB1 NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AbsI | CCTCGAGG | 1 cut(s) | 236 |
| AccB1I | GGYRCC | 1 cut(s) | 229 |
| AclWI | GGATC | 1 cut(s) | 250 |
| AcsI | RAATTY | 1 cut(s) | 200 |
| AfaI | GTAC | 1 cut(s) | 69 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 239 |
| AgsI | TTSAA | 2 cut(s) | 103, 267 |
| AluBI | AGCT | 2 cut(s) | 109, 284 |
| AluI | AGCT | 2 cut(s) | 109, 284 |
| Alw21I | GWGCWC | 1 cut(s) | 276 |
| Alw26I | GTCTC | 2 cut(s) | 8, 37 |
| AlwI | GGATC | 1 cut(s) | 250 |
| Ama87I | CYCGRG | 1 cut(s) | 236 |
| ApoI | RAATTY | 1 cut(s) | 200 |
| AsuHPI | GGTGA | 1 cut(s) | 236 |
| AvaI | CYCGRG | 1 cut(s) | 236 |
| BanI | GGYRCC | 1 cut(s) | 229 |
| Bbv12I | GWGCWC | 1 cut(s) | 276 |
| BcoDI | GTCTC | 2 cut(s) | 8, 37 |
| BfaI | CTAG | 5 cut(s) | 81, 120, 140, 206, 281 |
| BfmI | CTRYAG | 2 cut(s) | 129, 148 |
| BmeT110I | CYCGRG | 1 cut(s) | 236 |
| BmiI | GGNNCC | 1 cut(s) | 231 |
| BmrI | ACTGGG | 1 cut(s) | 54 |
| BmuI | ACTGGG | 1 cut(s) | 54 |
| BpuEI | CTTGAG | 1 cut(s) | 209 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 239 |
| Bse1I | ACTGG | 1 cut(s) | 60 |
| BseGI | GGATG | 1 cut(s) | 129 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 239 |
| BseMII | CTCAG | 1 cut(s) | 261 |
| BseNI | ACTGG | 1 cut(s) | 60 |
| BshNI | GGYRCC | 1 cut(s) | 229 |
| BsiHKAI | GWGCWC | 1 cut(s) | 276 |
| BsiHKCI | CYCGRG | 1 cut(s) | 236 |
| BslFI | GGGAC | 1 cut(s) | 42 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 239 |
| BsmAI | GTCTC | 2 cut(s) | 8, 37 |
| BsmFI | GGGAC | 1 cut(s) | 42 |
| BsoBI | CYCGRG | 1 cut(s) | 236 |
| Bsp1286I | GDGCHC | 1 cut(s) | 276 |
| Bsp143I | GATC | 2 cut(s) | 136, 242 |
| BspCNI | CTCAG | 2 cut(s) | 4, 262 |
| BspLI | GGNNCC | 1 cut(s) | 231 |
| BspMAI | CTGCAG | 1 cut(s) | 133 |
| BspPI | GGATC | 1 cut(s) | 250 |
| BspT107I | GGYRCC | 1 cut(s) | 229 |
| BsrI | ACTGG | 1 cut(s) | 60 |
| BssMI | GATC | 2 cut(s) | 136, 242 |
| Bst4CI | ACNGT | 1 cut(s) | 152 |
| BstDEI | CTNAG | 2 cut(s) | 12, 270 |
| BstF5I | GGATG | 1 cut(s) | 129 |
| BstKTI | GATC | 2 cut(s) | 139, 245 |
| BstMAI | GTCTC | 2 cut(s) | 8, 37 |
| BstMBI | GATC | 2 cut(s) | 136, 242 |
| BstSFI | CTRYAG | 2 cut(s) | 129, 148 |
| BtsCI | GGATG | 1 cut(s) | 129 |
| BtsIMutI | CAGTG | 2 cut(s) | 67, 157 |
| Csp6I | GTAC | 1 cut(s) | 68 |
| CviJI | RGCY | 2 cut(s) | 109, 284 |
| CviKI_1 | RGCY | 2 cut(s) | 109, 284 |
| CviQI | GTAC | 1 cut(s) | 68 |
| DdeI | CTNAG | 2 cut(s) | 12, 270 |
| DpnI | GATC | 2 cut(s) | 138, 244 |
| DpnII | GATC | 2 cut(s) | 136, 242 |
| DraI | TTTAAA | 1 cut(s) | 174 |
| Eco88I | CYCGRG | 1 cut(s) | 236 |
| EcoRI | GAATTC | 1 cut(s) | 200 |
| FaiI | YATR | 1 cut(s) | 257 |
| FaqI | GGGAC | 1 cut(s) | 42 |
| FokI | GGATG | 1 cut(s) | 136 |
| FspBI | CTAG | 5 cut(s) | 81, 120, 140, 206, 281 |
| HphI | GGTGA | 1 cut(s) | 236 |
| Hpy166II | GTNNAC | 1 cut(s) | 68 |
| Hpy188I | TCNGA | 2 cut(s) | 13, 48 |
| Hpy188III | TCNNGA | 1 cut(s) | 140 |
| Hpy8I | GTNNAC | 1 cut(s) | 68 |
| HpyAV | CCTTC | 1 cut(s) | 209 |
| HpyCH4III | ACNGT | 1 cut(s) | 152 |
| HpyCH4V | TGCA | 1 cut(s) | 131 |
| HpyF3I | CTNAG | 2 cut(s) | 12, 270 |
| Kzo9I | GATC | 2 cut(s) | 136, 242 |
| LmnI | GCTCC | 1 cut(s) | 106 |
| LpnPI | CCDG | 2 cut(s) | 73, 264 |
| MaeI | CTAG | 5 cut(s) | 81, 120, 140, 206, 281 |
| MalI | GATC | 2 cut(s) | 138, 244 |
| MboI | GATC | 2 cut(s) | 136, 242 |
| MboII | GAAGA | 1 cut(s) | 255 |
| MhlI | GDGCHC | 1 cut(s) | 276 |
| MluCI | AATT | 2 cut(s) | 194, 200 |
| MmeI | TCCRAC | 1 cut(s) | 98 |
| MnlI | CCTC | 4 cut(s) | 184, 220, 232, 245 |
| MseI | TTAA | 1 cut(s) | 173 |
| NdeII | GATC | 2 cut(s) | 136, 242 |
| NlaIV | GGNNCC | 1 cut(s) | 231 |
| PaeR7I | CTCGAG | 1 cut(s) | 236 |
| PspN4I | GGNNCC | 1 cut(s) | 231 |
| PspXI | VCTCGAGB | 1 cut(s) | 236 |
| PstI | CTGCAG | 1 cut(s) | 133 |
| RsaI | GTAC | 1 cut(s) | 69 |
| RsaNI | GTAC | 1 cut(s) | 68 |
| SaqAI | TTAA | 1 cut(s) | 173 |
| Sau3AI | GATC | 2 cut(s) | 136, 242 |
| SduI | GDGCHC | 1 cut(s) | 276 |
| SetI | ASST | 4 cut(s) | 111, 220, 286, 299 |
| SfcI | CTRYAG | 2 cut(s) | 129, 148 |
| Sfr274I | CTCGAG | 1 cut(s) | 236 |
| SlaI | CTCGAG | 1 cut(s) | 236 |
| SmlI | CTYRAG | 2 cut(s) | 188, 236 |
| SmoI | CTYRAG | 2 cut(s) | 188, 236 |
| Sse9I | AATT | 2 cut(s) | 194, 200 |
| SspMI | CTAG | 5 cut(s) | 81, 120, 140, 206, 281 |
| TaaI | ACNGT | 1 cut(s) | 152 |
| TaqI | TCGA | 1 cut(s) | 237 |
| TasI | AATT | 2 cut(s) | 194, 200 |
| TatI | WGTACW | 1 cut(s) | 67 |
| Tru1I | TTAA | 1 cut(s) | 173 |
| Tru9I | TTAA | 1 cut(s) | 173 |
| TscAI | CASTG | 2 cut(s) | 67, 157 |
| TspDTI | ATGAA | 1 cut(s) | 228 |
| TspRI | CASTG | 2 cut(s) | 67, 157 |
| XapI | RAATTY | 1 cut(s) | 200 |
| XbaI | TCTAGA | 1 cut(s) | 139 |
| XhoI | CTCGAG | 1 cut(s) | 236 |
| XspI | CTAG | 5 cut(s) | 81, 120, 140, 206, 281 |