Regulatory subunit of the dimeric E1 enzyme. E1 activates RUB1 NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AbsI | CCTCGAGG | 1 cut(s) | 791 |
| AccB1I | GGYRCC | 1 cut(s) | 784 |
| AclWI | GGATC | 1 cut(s) | 805 |
| AcsI | RAATTY | 1 cut(s) | 755 |
| AfaI | GTAC | 2 cut(s) | 116, 624 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 794 |
| AgsI | TTSAA | 5 cut(s) | 211, 530, 592, 658, 822 |
| AjnI | CCWGG | 2 cut(s) | 289, 343 |
| AluBI | AGCT | 5 cut(s) | 487, 610, 664, 839, 866 |
| AluI | AGCT | 5 cut(s) | 487, 610, 664, 839, 866 |
| Alw21I | GWGCWC | 1 cut(s) | 831 |
| Alw26I | GTCTC | 1 cut(s) | 477 |
| AlwI | GGATC | 1 cut(s) | 805 |
| Ama87I | CYCGRG | 1 cut(s) | 791 |
| AoxI | GGCC | 2 cut(s) | 286, 602 |
| ApeKI | GCWGC | 2 cut(s) | 155, 484 |
| ApoI | RAATTY | 1 cut(s) | 755 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 570 |
| AspS9I | GGNCC | 1 cut(s) | 287 |
| AsuHPI | GGTGA | 4 cut(s) | 83, 275, 305, 791 |
| AvaI | CYCGRG | 1 cut(s) | 791 |
| BanI | GGYRCC | 1 cut(s) | 784 |
| Bbv12I | GWGCWC | 1 cut(s) | 831 |
| BbvCI | CCTCAGC | 1 cut(s) | 867 |
| BbvI | GCAGC | 2 cut(s) | 142, 496 |
| BciT130I | CCWGG | 2 cut(s) | 291, 345 |
| BclI | TGATCA | 2 cut(s) | 88, 381 |
| BcoDI | GTCTC | 1 cut(s) | 477 |
| BfaI | CTAG | 6 cut(s) | 78, 333, 636, 675, 761, 881 |
| BfmI | CTRYAG | 2 cut(s) | 684, 703 |
| BglII | AGATCT | 1 cut(s) | 512 |
| BisI | GCNGC | 2 cut(s) | 156, 485 |
| BlsI | GCNGC | 2 cut(s) | 157, 486 |
| Bme1390I | CCNGG | 2 cut(s) | 291, 345 |
| BmeT110I | CYCGRG | 1 cut(s) | 791 |
| BmgT120I | GGNCC | 1 cut(s) | 287 |
| BmiI | GGNNCC | 1 cut(s) | 786 |
| BmrFI | CCNGG | 2 cut(s) | 291, 345 |
| BmsI | GCATC | 1 cut(s) | 304 |
| Bpu10I | CCTNAGC | 2 cut(s) | 606, 867 |
| BpuEI | CTTGAG | 2 cut(s) | 200, 764 |
| BsaAI | YACGTR | 1 cut(s) | 835 |
| BsaJI | CCNNGG | 2 cut(s) | 289, 599 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 794 |
| Bse1I | ACTGG | 1 cut(s) | 73 |
| BseBI | CCWGG | 2 cut(s) | 291, 345 |
| BseDI | CCNNGG | 2 cut(s) | 289, 599 |
| BseGI | GGATG | 2 cut(s) | 583, 684 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 794 |
| BseMII | CTCAG | 3 cut(s) | 597, 816, 858 |
| BseNI | ACTGG | 1 cut(s) | 73 |
| BseRI | GAGGAG | 1 cut(s) | 197 |
| BseXI | GCAGC | 2 cut(s) | 142, 496 |
| BsgI | GTGCAG | 1 cut(s) | 49 |
| BshFI | GGCC | 2 cut(s) | 288, 604 |
| BshNI | GGYRCC | 1 cut(s) | 784 |
| BsiHKAI | GWGCWC | 1 cut(s) | 831 |
| BsiHKCI | CYCGRG | 1 cut(s) | 791 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 794 |
| BsmAI | GTCTC | 1 cut(s) | 477 |
| BsnI | GGCC | 2 cut(s) | 288, 604 |
| BsoBI | CYCGRG | 1 cut(s) | 791 |
| Bsp1286I | GDGCHC | 1 cut(s) | 831 |
| Bsp143I | GATC | 5 cut(s) | 22, 88, 381, 512, 797 |
| Bsp19I | CCATGG | 1 cut(s) | 599 |
| BspANI | GGCC | 2 cut(s) | 288, 604 |
| BspCNI | CTCAG | 3 cut(s) | 598, 817, 859 |
| BspLI | GGNNCC | 1 cut(s) | 786 |
| BspMAI | CTGCAG | 1 cut(s) | 688 |
| BspPI | GGATC | 1 cut(s) | 805 |
| BspT107I | GGYRCC | 1 cut(s) | 784 |
| BsrI | ACTGG | 1 cut(s) | 73 |
| BssECI | CCNNGG | 2 cut(s) | 289, 599 |
| BssMI | GATC | 5 cut(s) | 22, 88, 381, 512, 797 |
| BssT1I | CCWWGG | 1 cut(s) | 599 |
| Bst2UI | CCWGG | 2 cut(s) | 291, 345 |
| Bst4CI | ACNGT | 2 cut(s) | 547, 707 |
| Bst6I | CTCTTC | 1 cut(s) | 636 |
| BstBAI | YACGTR | 1 cut(s) | 835 |
| BstDEI | CTNAG | 4 cut(s) | 606, 611, 825, 867 |
| BstDSI | CCRYGG | 1 cut(s) | 599 |
| BstF5I | GGATG | 2 cut(s) | 583, 684 |
| BstKTI | GATC | 5 cut(s) | 25, 91, 384, 515, 800 |
| BstMAI | GTCTC | 1 cut(s) | 477 |
| BstMBI | GATC | 5 cut(s) | 22, 88, 381, 512, 797 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 155, 164 |
| BstNI | CCWGG | 2 cut(s) | 291, 345 |
| BstSCI | CCNGG | 2 cut(s) | 289, 343 |
| BstSFI | CTRYAG | 2 cut(s) | 684, 703 |
| BstSNI | TACGTA | 1 cut(s) | 835 |
| BstV1I | GCAGC | 2 cut(s) | 142, 496 |
| BstX2I | RGATCY | 1 cut(s) | 512 |
| BstYI | RGATCY | 1 cut(s) | 512 |
| BsuRI | GGCC | 2 cut(s) | 288, 604 |
| BtgI | CCRYGG | 1 cut(s) | 599 |
| BtsCI | GGATG | 2 cut(s) | 583, 684 |
| BtsI | GCAGTG | 1 cut(s) | 334 |
| BtsIMutI | CAGTG | 3 cut(s) | 66, 334, 712 |
| Cfr13I | GGNCC | 1 cut(s) | 287 |
| Csp6I | GTAC | 2 cut(s) | 115, 623 |
| CviAII | CATG | 3 cut(s) | 385, 427, 600 |
| CviQI | GTAC | 2 cut(s) | 115, 623 |
| DdeI | CTNAG | 4 cut(s) | 606, 611, 825, 867 |
| DpnI | GATC | 5 cut(s) | 24, 90, 383, 514, 799 |
| DpnII | GATC | 5 cut(s) | 22, 88, 381, 512, 797 |
| DraI | TTTAAA | 1 cut(s) | 729 |
| Eam1104I | CTCTTC | 1 cut(s) | 636 |
| EarI | CTCTTC | 1 cut(s) | 636 |
| Eco105I | TACGTA | 1 cut(s) | 835 |
| Eco130I | CCWWGG | 1 cut(s) | 599 |
| Eco88I | CYCGRG | 1 cut(s) | 791 |
| EcoO109I | RGGNCCY | 1 cut(s) | 287 |
| EcoRI | GAATTC | 1 cut(s) | 755 |
| EcoRII | CCWGG | 2 cut(s) | 289, 343 |
| EcoT14I | CCWWGG | 1 cut(s) | 599 |
| ErhI | CCWWGG | 1 cut(s) | 599 |
| FaeI | CATG | 3 cut(s) | 388, 430, 603 |
| FatI | CATG | 3 cut(s) | 384, 426, 599 |
| FbaI | TGATCA | 2 cut(s) | 88, 381 |
| Fnu4HI | GCNGC | 2 cut(s) | 156, 485 |
| FokI | GGATG | 2 cut(s) | 590, 691 |
| Fsp4HI | GCNGC | 2 cut(s) | 156, 485 |
| FspBI | CTAG | 6 cut(s) | 78, 333, 636, 675, 761, 881 |
| GluI | GCNGC | 2 cut(s) | 156, 485 |
| HaeIII | GGCC | 2 cut(s) | 288, 604 |
| Hin1II | CATG | 3 cut(s) | 388, 430, 603 |
| HinfI | GANTC | 6 cut(s) | 60, 266, 340, 431, 554, 903 |
| HphI | GGTGA | 4 cut(s) | 83, 275, 305, 791 |
| Hpy166II | GTNNAC | 1 cut(s) | 623 |
| Hpy188I | TCNGA | 1 cut(s) | 198 |
| Hpy188III | TCNNGA | 4 cut(s) | 34, 179, 379, 575 |
| Hpy8I | GTNNAC | 1 cut(s) | 623 |
| HpyAV | CCTTC | 3 cut(s) | 433, 524, 764 |
| HpyCH4III | ACNGT | 2 cut(s) | 547, 707 |
| HpyCH4IV | ACGT | 4 cut(s) | 117, 224, 582, 834 |
| HpyCH4V | TGCA | 3 cut(s) | 66, 327, 686 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 155, 164 |
| HpyF3I | CTNAG | 4 cut(s) | 606, 611, 825, 867 |
| HpySE526I | ACGT | 4 cut(s) | 117, 224, 582, 834 |
| Hsp92II | CATG | 3 cut(s) | 388, 430, 603 |
| Ksp22I | TGATCA | 2 cut(s) | 88, 381 |
| Kzo9I | GATC | 5 cut(s) | 22, 88, 381, 512, 797 |
| LmnI | GCTCC | 1 cut(s) | 661 |
| Lsp1109I | GCAGC | 2 cut(s) | 142, 496 |
| LweI | GCATC | 1 cut(s) | 304 |
| MaeI | CTAG | 6 cut(s) | 78, 333, 636, 675, 761, 881 |
| MaeII | ACGT | 4 cut(s) | 117, 224, 582, 834 |
| MaeIII | GTNAC | 4 cut(s) | 225, 267, 277, 293 |
| MalI | GATC | 5 cut(s) | 24, 90, 383, 514, 799 |
| MboI | GATC | 5 cut(s) | 22, 88, 381, 512, 797 |
| MboII | GAAGA | 4 cut(s) | 223, 653, 810, 898 |
| MflI | RGATCY | 1 cut(s) | 512 |
| MhlI | GDGCHC | 1 cut(s) | 831 |
| MluCI | AATT | 4 cut(s) | 322, 371, 749, 755 |
| MlyI | GAGTC | 4 cut(s) | 275, 425, 563, 897 |
| MmeI | TCCRAC | 1 cut(s) | 653 |
| MroXI | GAANNNNTTC | 1 cut(s) | 570 |
| MseI | TTAA | 2 cut(s) | 537, 728 |
| MspR9I | CCNGG | 2 cut(s) | 291, 345 |
| MvaI | CCWGG | 2 cut(s) | 291, 345 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 155, 164 |
| NcoI | CCATGG | 1 cut(s) | 599 |
| NdeII | GATC | 5 cut(s) | 22, 88, 381, 512, 797 |
| NlaIII | CATG | 3 cut(s) | 388, 430, 603 |
| NlaIV | GGNNCC | 1 cut(s) | 786 |
| NmuCI | GTSAC | 3 cut(s) | 267, 277, 293 |
| PaeR7I | CTCGAG | 1 cut(s) | 791 |
| PdmI | GAANNNNTTC | 1 cut(s) | 570 |
| PfeI | GAWTC | 2 cut(s) | 60, 340 |
| PfoI | TCCNGGA | 1 cut(s) | 343 |
| PkrI | GCNGC | 2 cut(s) | 157, 486 |
| PleI | GAGTC | 4 cut(s) | 274, 425, 562, 897 |
| PpsI | GAGTC | 4 cut(s) | 274, 425, 562, 897 |
| Ppu21I | YACGTR | 1 cut(s) | 835 |
| Psp6I | CCWGG | 2 cut(s) | 289, 343 |
| PspGI | CCWGG | 2 cut(s) | 289, 343 |
| PspN4I | GGNNCC | 1 cut(s) | 786 |
| PspPI | GGNCC | 1 cut(s) | 287 |
| PspXI | VCTCGAGB | 1 cut(s) | 791 |
| PstI | CTGCAG | 1 cut(s) | 688 |
| PsuI | RGATCY | 1 cut(s) | 512 |
| RsaI | GTAC | 2 cut(s) | 116, 624 |
| RsaNI | GTAC | 2 cut(s) | 115, 623 |
| SaqAI | TTAA | 2 cut(s) | 537, 728 |
| SatI | GCNGC | 2 cut(s) | 156, 485 |
| Sau3AI | GATC | 5 cut(s) | 22, 88, 381, 512, 797 |
| Sau96I | GGNCC | 1 cut(s) | 287 |
| SchI | GAGTC | 4 cut(s) | 275, 425, 563, 897 |
| ScrFI | CCNGG | 2 cut(s) | 291, 345 |
| SduI | GDGCHC | 1 cut(s) | 831 |
| SfaNI | GCATC | 1 cut(s) | 304 |
| SfcI | CTRYAG | 2 cut(s) | 684, 703 |
| Sfr274I | CTCGAG | 1 cut(s) | 791 |
| SlaI | CTCGAG | 1 cut(s) | 791 |
| SmlI | CTYRAG | 3 cut(s) | 179, 743, 791 |
| SmoI | CTYRAG | 3 cut(s) | 179, 743, 791 |
| SnaBI | TACGTA | 1 cut(s) | 835 |
| Sse9I | AATT | 4 cut(s) | 322, 371, 749, 755 |
| SspI | AATATT | 1 cut(s) | 45 |
| SspMI | CTAG | 6 cut(s) | 78, 333, 636, 675, 761, 881 |
| StyD4I | CCNGG | 2 cut(s) | 289, 343 |
| StyI | CCWWGG | 1 cut(s) | 599 |
| TaaI | ACNGT | 2 cut(s) | 547, 707 |
| TaiI | ACGT | 4 cut(s) | 120, 227, 585, 837 |
| TaqI | TCGA | 2 cut(s) | 450, 792 |
| TasI | AATT | 4 cut(s) | 322, 371, 749, 755 |
| TatI | WGTACW | 1 cut(s) | 622 |
| TfiI | GAWTC | 2 cut(s) | 60, 340 |
| Tru1I | TTAA | 2 cut(s) | 537, 728 |
| Tru9I | TTAA | 2 cut(s) | 537, 728 |
| TscAI | CASTG | 3 cut(s) | 73, 334, 712 |
| TseFI | GTSAC | 3 cut(s) | 267, 277, 293 |
| TseI | GCWGC | 2 cut(s) | 155, 484 |
| Tsp45I | GTSAC | 3 cut(s) | 267, 277, 293 |
| TspDTI | ATGAA | 2 cut(s) | 415, 783 |
| TspRI | CASTG | 3 cut(s) | 73, 334, 712 |
| XapI | RAATTY | 1 cut(s) | 755 |
| XhoI | CTCGAG | 1 cut(s) | 791 |
| XmnI | GAANNNNTTC | 1 cut(s) | 570 |
| XspI | CTAG | 6 cut(s) | 78, 333, 636, 675, 761, 881 |