Regulatory subunit of the dimeric E1 enzyme. E1 activates RUB1 NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AbsI | CCTCGAGG | 1 cut(s) | 551 |
| AccB1I | GGYRCC | 1 cut(s) | 544 |
| AccI | GTMKAC | 1 cut(s) | 5 |
| AclWI | GGATC | 2 cut(s) | 274, 565 |
| AcsI | RAATTY | 1 cut(s) | 515 |
| AfaI | GTAC | 1 cut(s) | 384 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 554 |
| AgsI | TTSAA | 4 cut(s) | 284, 346, 418, 582 |
| AjnI | CCWGG | 2 cut(s) | 43, 97 |
| AluBI | AGCT | 5 cut(s) | 241, 364, 424, 599, 626 |
| AluI | AGCT | 5 cut(s) | 241, 364, 424, 599, 626 |
| Alw21I | GWGCWC | 1 cut(s) | 591 |
| Alw26I | GTCTC | 1 cut(s) | 231 |
| AlwI | GGATC | 2 cut(s) | 274, 565 |
| Ama87I | CYCGRG | 1 cut(s) | 551 |
| AoxI | GGCC | 2 cut(s) | 40, 356 |
| ApeKI | GCWGC | 1 cut(s) | 238 |
| ApoI | RAATTY | 1 cut(s) | 515 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 324 |
| AspS9I | GGNCC | 1 cut(s) | 41 |
| AsuHPI | GGTGA | 3 cut(s) | 29, 59, 551 |
| AvaI | CYCGRG | 1 cut(s) | 551 |
| BanI | GGYRCC | 1 cut(s) | 544 |
| Bbv12I | GWGCWC | 1 cut(s) | 591 |
| BbvCI | CCTCAGC | 1 cut(s) | 627 |
| BbvI | GCAGC | 1 cut(s) | 250 |
| BciT130I | CCWGG | 2 cut(s) | 45, 99 |
| BclI | TGATCA | 1 cut(s) | 135 |
| BcoDI | GTCTC | 1 cut(s) | 231 |
| BfaI | CTAG | 5 cut(s) | 87, 396, 435, 521, 641 |
| BfmI | CTRYAG | 2 cut(s) | 444, 463 |
| BisI | GCNGC | 1 cut(s) | 239 |
| BlsI | GCNGC | 1 cut(s) | 240 |
| Bme1390I | CCNGG | 2 cut(s) | 45, 99 |
| BmeT110I | CYCGRG | 1 cut(s) | 551 |
| BmgT120I | GGNCC | 1 cut(s) | 41 |
| BmiI | GGNNCC | 1 cut(s) | 546 |
| BmrFI | CCNGG | 2 cut(s) | 45, 99 |
| BmrI | ACTGGG | 1 cut(s) | 369 |
| BmsI | GCATC | 1 cut(s) | 58 |
| BmuI | ACTGGG | 1 cut(s) | 369 |
| Bpu10I | CCTNAGC | 2 cut(s) | 360, 627 |
| BpuEI | CTTGAG | 1 cut(s) | 524 |
| BsaJI | CCNNGG | 2 cut(s) | 43, 353 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 554 |
| Bse1I | ACTGG | 1 cut(s) | 375 |
| BseBI | CCWGG | 2 cut(s) | 45, 99 |
| BseDI | CCNNGG | 2 cut(s) | 43, 353 |
| BseGI | GGATG | 2 cut(s) | 337, 444 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 554 |
| BseMII | CTCAG | 3 cut(s) | 351, 576, 618 |
| BseNI | ACTGG | 1 cut(s) | 375 |
| BseXI | GCAGC | 1 cut(s) | 250 |
| BshFI | GGCC | 2 cut(s) | 42, 358 |
| BshNI | GGYRCC | 1 cut(s) | 544 |
| BsiHKAI | GWGCWC | 1 cut(s) | 591 |
| BsiHKCI | CYCGRG | 1 cut(s) | 551 |
| BslFI | GGGAC | 1 cut(s) | 357 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 554 |
| BsmAI | GTCTC | 1 cut(s) | 231 |
| BsmFI | GGGAC | 1 cut(s) | 357 |
| BsnI | GGCC | 2 cut(s) | 42, 358 |
| BsoBI | CYCGRG | 1 cut(s) | 551 |
| Bsp1286I | GDGCHC | 1 cut(s) | 591 |
| Bsp143I | GATC | 3 cut(s) | 135, 266, 557 |
| Bsp19I | CCATGG | 1 cut(s) | 353 |
| BspANI | GGCC | 2 cut(s) | 42, 358 |
| BspCNI | CTCAG | 3 cut(s) | 352, 577, 619 |
| BspLI | GGNNCC | 1 cut(s) | 546 |
| BspMAI | CTGCAG | 1 cut(s) | 448 |
| BspPI | GGATC | 2 cut(s) | 274, 565 |
| BspT107I | GGYRCC | 1 cut(s) | 544 |
| BsrI | ACTGG | 1 cut(s) | 375 |
| BssECI | CCNNGG | 2 cut(s) | 43, 353 |
| BssMI | GATC | 3 cut(s) | 135, 266, 557 |
| BssT1I | CCWWGG | 1 cut(s) | 353 |
| Bst2UI | CCWGG | 2 cut(s) | 45, 99 |
| Bst4CI | ACNGT | 2 cut(s) | 301, 467 |
| Bst6I | CTCTTC | 1 cut(s) | 396 |
| BstDEI | CTNAG | 4 cut(s) | 360, 365, 585, 627 |
| BstDSI | CCRYGG | 1 cut(s) | 353 |
| BstF5I | GGATG | 2 cut(s) | 337, 444 |
| BstKTI | GATC | 3 cut(s) | 138, 269, 560 |
| BstMAI | GTCTC | 1 cut(s) | 231 |
| BstMBI | GATC | 3 cut(s) | 135, 266, 557 |
| BstNI | CCWGG | 2 cut(s) | 45, 99 |
| BstSCI | CCNGG | 2 cut(s) | 43, 97 |
| BstSFI | CTRYAG | 2 cut(s) | 444, 463 |
| BstV1I | GCAGC | 1 cut(s) | 250 |
| BstX2I | RGATCY | 1 cut(s) | 266 |
| BstYI | RGATCY | 1 cut(s) | 266 |
| BsuRI | GGCC | 2 cut(s) | 42, 358 |
| BtgI | CCRYGG | 1 cut(s) | 353 |
| BtsCI | GGATG | 2 cut(s) | 337, 444 |
| BtsI | GCAGTG | 1 cut(s) | 88 |
| BtsIMutI | CAGTG | 3 cut(s) | 88, 382, 472 |
| Cfr13I | GGNCC | 1 cut(s) | 41 |
| Csp6I | GTAC | 1 cut(s) | 383 |
| CviAII | CATG | 3 cut(s) | 139, 181, 354 |
| CviQI | GTAC | 1 cut(s) | 383 |
| DdeI | CTNAG | 4 cut(s) | 360, 365, 585, 627 |
| DpnI | GATC | 3 cut(s) | 137, 268, 559 |
| DpnII | GATC | 3 cut(s) | 135, 266, 557 |
| DraI | TTTAAA | 1 cut(s) | 489 |
| Eam1104I | CTCTTC | 1 cut(s) | 396 |
| EarI | CTCTTC | 1 cut(s) | 396 |
| Eco130I | CCWWGG | 1 cut(s) | 353 |
| Eco88I | CYCGRG | 1 cut(s) | 551 |
| EcoO109I | RGGNCCY | 1 cut(s) | 41 |
| EcoRI | GAATTC | 1 cut(s) | 515 |
| EcoRII | CCWGG | 2 cut(s) | 43, 97 |
| EcoT14I | CCWWGG | 1 cut(s) | 353 |
| ErhI | CCWWGG | 1 cut(s) | 353 |
| FaeI | CATG | 3 cut(s) | 142, 184, 357 |
| FaqI | GGGAC | 1 cut(s) | 357 |
| FatI | CATG | 3 cut(s) | 138, 180, 353 |
| FbaI | TGATCA | 1 cut(s) | 135 |
| FblI | GTMKAC | 1 cut(s) | 5 |
| Fnu4HI | GCNGC | 1 cut(s) | 239 |
| FokI | GGATG | 2 cut(s) | 344, 451 |
| Fsp4HI | GCNGC | 1 cut(s) | 239 |
| FspBI | CTAG | 5 cut(s) | 87, 396, 435, 521, 641 |
| GluI | GCNGC | 1 cut(s) | 239 |
| HaeIII | GGCC | 2 cut(s) | 42, 358 |
| Hin1II | CATG | 3 cut(s) | 142, 184, 357 |
| HinfI | GANTC | 5 cut(s) | 20, 94, 185, 308, 663 |
| HphI | GGTGA | 3 cut(s) | 29, 59, 551 |
| Hpy166II | GTNNAC | 2 cut(s) | 6, 383 |
| Hpy188III | TCNNGA | 2 cut(s) | 133, 329 |
| Hpy8I | GTNNAC | 2 cut(s) | 6, 383 |
| HpyAV | CCTTC | 4 cut(s) | 187, 278, 347, 524 |
| HpyCH4III | ACNGT | 2 cut(s) | 301, 467 |
| HpyCH4V | TGCA | 2 cut(s) | 81, 446 |
| HpyF3I | CTNAG | 4 cut(s) | 360, 365, 585, 627 |
| Hsp92II | CATG | 3 cut(s) | 142, 184, 357 |
| Ksp22I | TGATCA | 1 cut(s) | 135 |
| Kzo9I | GATC | 3 cut(s) | 135, 266, 557 |
| LmnI | GCTCC | 1 cut(s) | 421 |
| Lsp1109I | GCAGC | 1 cut(s) | 250 |
| LweI | GCATC | 1 cut(s) | 58 |
| MaeI | CTAG | 5 cut(s) | 87, 396, 435, 521, 641 |
| MaeIII | GTNAC | 3 cut(s) | 21, 31, 47 |
| MalI | GATC | 3 cut(s) | 137, 268, 559 |
| MboI | GATC | 3 cut(s) | 135, 266, 557 |
| MboII | GAAGA | 3 cut(s) | 413, 570, 658 |
| MflI | RGATCY | 1 cut(s) | 266 |
| MhlI | GDGCHC | 1 cut(s) | 591 |
| MluCI | AATT | 4 cut(s) | 76, 125, 509, 515 |
| MlyI | GAGTC | 4 cut(s) | 29, 179, 317, 657 |
| MmeI | TCCRAC | 1 cut(s) | 413 |
| MnlI | CCTC | 9 cut(s) | 140, 146, 175, 499, 530, 535, 547, 560, 622 |
| MroXI | GAANNNNTTC | 1 cut(s) | 324 |
| MseI | TTAA | 2 cut(s) | 291, 488 |
| MspR9I | CCNGG | 2 cut(s) | 45, 99 |
| MvaI | CCWGG | 2 cut(s) | 45, 99 |
| NcoI | CCATGG | 1 cut(s) | 353 |
| NdeII | GATC | 3 cut(s) | 135, 266, 557 |
| NlaIII | CATG | 3 cut(s) | 142, 184, 357 |
| NlaIV | GGNNCC | 1 cut(s) | 546 |
| NmuCI | GTSAC | 3 cut(s) | 21, 31, 47 |
| PaeR7I | CTCGAG | 1 cut(s) | 551 |
| PdmI | GAANNNNTTC | 1 cut(s) | 324 |
| PfeI | GAWTC | 1 cut(s) | 94 |
| PfoI | TCCNGGA | 1 cut(s) | 97 |
| PkrI | GCNGC | 1 cut(s) | 240 |
| PleI | GAGTC | 4 cut(s) | 28, 179, 316, 657 |
| PpsI | GAGTC | 4 cut(s) | 28, 179, 316, 657 |
| Psp6I | CCWGG | 2 cut(s) | 43, 97 |
| PspGI | CCWGG | 2 cut(s) | 43, 97 |
| PspN4I | GGNNCC | 1 cut(s) | 546 |
| PspPI | GGNCC | 1 cut(s) | 41 |
| PspXI | VCTCGAGB | 1 cut(s) | 551 |
| PstI | CTGCAG | 1 cut(s) | 448 |
| PsuI | RGATCY | 1 cut(s) | 266 |
| RsaI | GTAC | 1 cut(s) | 384 |
| RsaNI | GTAC | 1 cut(s) | 383 |
| SaqAI | TTAA | 2 cut(s) | 291, 488 |
| SatI | GCNGC | 1 cut(s) | 239 |
| Sau3AI | GATC | 3 cut(s) | 135, 266, 557 |
| Sau96I | GGNCC | 1 cut(s) | 41 |
| SchI | GAGTC | 4 cut(s) | 29, 179, 317, 657 |
| ScrFI | CCNGG | 2 cut(s) | 45, 99 |
| SduI | GDGCHC | 1 cut(s) | 591 |
| SfaNI | GCATC | 1 cut(s) | 58 |
| SfcI | CTRYAG | 2 cut(s) | 444, 463 |
| Sfr274I | CTCGAG | 1 cut(s) | 551 |
| SlaI | CTCGAG | 1 cut(s) | 551 |
| SmlI | CTYRAG | 2 cut(s) | 503, 551 |
| SmoI | CTYRAG | 2 cut(s) | 503, 551 |
| Sse9I | AATT | 4 cut(s) | 76, 125, 509, 515 |
| SspMI | CTAG | 5 cut(s) | 87, 396, 435, 521, 641 |
| StyD4I | CCNGG | 2 cut(s) | 43, 97 |
| StyI | CCWWGG | 1 cut(s) | 353 |
| TaaI | ACNGT | 2 cut(s) | 301, 467 |
| TaqI | TCGA | 2 cut(s) | 204, 552 |
| TasI | AATT | 4 cut(s) | 76, 125, 509, 515 |
| TatI | WGTACW | 1 cut(s) | 382 |
| TfiI | GAWTC | 1 cut(s) | 94 |
| Tru1I | TTAA | 2 cut(s) | 291, 488 |
| Tru9I | TTAA | 2 cut(s) | 291, 488 |
| TscAI | CASTG | 3 cut(s) | 88, 382, 472 |
| TseFI | GTSAC | 3 cut(s) | 21, 31, 47 |
| TseI | GCWGC | 1 cut(s) | 238 |
| Tsp45I | GTSAC | 3 cut(s) | 21, 31, 47 |
| TspDTI | ATGAA | 2 cut(s) | 169, 543 |
| TspGWI | ACGGA | 1 cut(s) | 279 |
| TspRI | CASTG | 3 cut(s) | 88, 382, 472 |
| XapI | RAATTY | 1 cut(s) | 515 |
| XhoI | CTCGAG | 1 cut(s) | 551 |
| XmiI | GTMKAC | 1 cut(s) | 5 |
| XmnI | GAANNNNTTC | 1 cut(s) | 324 |
| XspI | CTAG | 5 cut(s) | 87, 396, 435, 521, 641 |