Regulatory subunit of the dimeric E1 enzyme. E1 activates RUB1 NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 226 |
| AclWI | GGATC | 1 cut(s) | 247 |
| AcsI | RAATTY | 1 cut(s) | 197 |
| AfaI | GTAC | 2 cut(s) | 98, 246 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 236, 304 |
| AgsI | TTSAA | 2 cut(s) | 70, 264 |
| AluBI | AGCT | 4 cut(s) | 88, 106, 281, 382 |
| AluI | AGCT | 4 cut(s) | 88, 106, 281, 382 |
| Alw21I | GWGCWC | 1 cut(s) | 273 |
| AlwI | GGATC | 1 cut(s) | 247 |
| AoxI | GGCC | 1 cut(s) | 80 |
| ApeKI | GCWGC | 1 cut(s) | 374 |
| ApoI | RAATTY | 1 cut(s) | 197 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 48 |
| AsuHPI | GGTGA | 1 cut(s) | 233 |
| BanI | GGYRCC | 1 cut(s) | 226 |
| BbsI | GAAGAC | 1 cut(s) | 346 |
| Bbv12I | GWGCWC | 1 cut(s) | 273 |
| BbvCI | CCTCAGC | 1 cut(s) | 309 |
| BbvI | GCAGC | 1 cut(s) | 361 |
| BfaI | CTAG | 2 cut(s) | 117, 203 |
| BfmI | CTRYAG | 2 cut(s) | 126, 145 |
| BisI | GCNGC | 1 cut(s) | 375 |
| BlpI | GCTNAGC | 1 cut(s) | 378 |
| BlsI | GCNGC | 1 cut(s) | 376 |
| BmiI | GGNNCC | 1 cut(s) | 228 |
| BmsI | GCATC | 1 cut(s) | 351 |
| BpiI | GAAGAC | 1 cut(s) | 346 |
| Bpu10I | CCTNAGC | 2 cut(s) | 84, 309 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 378 |
| BpuEI | CTTGAG | 2 cut(s) | 206, 254 |
| BsaJI | CCNNGG | 3 cut(s) | 77, 303, 344 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 236, 304 |
| BseDI | CCNNGG | 3 cut(s) | 77, 303, 344 |
| BseGI | GGATG | 2 cut(s) | 61, 126 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 236, 304 |
| BseMII | CTCAG | 4 cut(s) | 75, 258, 300, 392 |
| BseXI | GCAGC | 1 cut(s) | 361 |
| BshFI | GGCC | 1 cut(s) | 82 |
| BshNI | GGYRCC | 1 cut(s) | 226 |
| BsiHKAI | GWGCWC | 1 cut(s) | 273 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 236, 304 |
| BsnI | GGCC | 1 cut(s) | 82 |
| Bsp1286I | GDGCHC | 1 cut(s) | 273 |
| Bsp143I | GATC | 1 cut(s) | 239 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 378 |
| Bsp19I | CCATGG | 1 cut(s) | 77 |
| BspANI | GGCC | 1 cut(s) | 82 |
| BspCNI | CTCAG | 4 cut(s) | 76, 259, 301, 391 |
| BspLI | GGNNCC | 1 cut(s) | 228 |
| BspMAI | CTGCAG | 1 cut(s) | 130 |
| BspPI | GGATC | 1 cut(s) | 247 |
| BspT107I | GGYRCC | 1 cut(s) | 226 |
| BssECI | CCNNGG | 3 cut(s) | 77, 303, 344 |
| BssMI | GATC | 1 cut(s) | 239 |
| BssT1I | CCWWGG | 3 cut(s) | 77, 303, 344 |
| Bst4CI | ACNGT | 3 cut(s) | 25, 149, 352 |
| BstDEI | CTNAG | 5 cut(s) | 84, 89, 267, 309, 378 |
| BstDSI | CCRYGG | 1 cut(s) | 77 |
| BstF5I | GGATG | 2 cut(s) | 61, 126 |
| BstKTI | GATC | 1 cut(s) | 242 |
| BstMBI | GATC | 1 cut(s) | 239 |
| BstSFI | CTRYAG | 2 cut(s) | 126, 145 |
| BstV1I | GCAGC | 1 cut(s) | 361 |
| BstV2I | GAAGAC | 1 cut(s) | 346 |
| BsuRI | GGCC | 1 cut(s) | 82 |
| BtgI | CCRYGG | 1 cut(s) | 77 |
| BtsCI | GGATG | 2 cut(s) | 61, 126 |
| BtsIMutI | CAGTG | 1 cut(s) | 154 |
| Csp6I | GTAC | 2 cut(s) | 97, 245 |
| CviAII | CATG | 1 cut(s) | 78 |
| CviQI | GTAC | 2 cut(s) | 97, 245 |
| DdeI | CTNAG | 5 cut(s) | 84, 89, 267, 309, 378 |
| DpnI | GATC | 1 cut(s) | 241 |
| DpnII | GATC | 1 cut(s) | 239 |
| DraI | TTTAAA | 2 cut(s) | 16, 171 |
| Eco130I | CCWWGG | 3 cut(s) | 77, 303, 344 |
| EcoRI | GAATTC | 1 cut(s) | 197 |
| EcoT14I | CCWWGG | 3 cut(s) | 77, 303, 344 |
| ErhI | CCWWGG | 3 cut(s) | 77, 303, 344 |
| FaeI | CATG | 1 cut(s) | 81 |
| FaiI | YATR | 6 cut(s) | 21, 79, 254, 276, 405, 443 |
| FatI | CATG | 1 cut(s) | 77 |
| Fnu4HI | GCNGC | 1 cut(s) | 375 |
| FokI | GGATG | 2 cut(s) | 68, 133 |
| Fsp4HI | GCNGC | 1 cut(s) | 375 |
| FspBI | CTAG | 2 cut(s) | 117, 203 |
| GluI | GCNGC | 1 cut(s) | 375 |
| HaeIII | GGCC | 1 cut(s) | 82 |
| Hin1II | CATG | 1 cut(s) | 81 |
| HinfI | GANTC | 2 cut(s) | 32, 426 |
| HphI | GGTGA | 1 cut(s) | 233 |
| Hpy188III | TCNNGA | 2 cut(s) | 53, 358 |
| HpyAV | CCTTC | 3 cut(s) | 71, 87, 206 |
| HpyCH4III | ACNGT | 3 cut(s) | 25, 149, 352 |
| HpyCH4V | TGCA | 1 cut(s) | 128 |
| HpyF3I | CTNAG | 5 cut(s) | 84, 89, 267, 309, 378 |
| Hsp92II | CATG | 1 cut(s) | 81 |
| Kzo9I | GATC | 1 cut(s) | 239 |
| LmnI | GCTCC | 1 cut(s) | 387 |
| LpnPI | CCDG | 4 cut(s) | 38, 54, 96, 261 |
| Lsp1109I | GCAGC | 1 cut(s) | 361 |
| LweI | GCATC | 1 cut(s) | 351 |
| MaeI | CTAG | 2 cut(s) | 117, 203 |
| MalI | GATC | 1 cut(s) | 241 |
| MboI | GATC | 1 cut(s) | 239 |
| MboII | GAAGA | 2 cut(s) | 252, 346 |
| MhlI | GDGCHC | 1 cut(s) | 273 |
| MluCI | AATT | 2 cut(s) | 191, 197 |
| MlyI | GAGTC | 1 cut(s) | 41 |
| MnlI | CCTC | 5 cut(s) | 181, 217, 229, 304, 457 |
| MroXI | GAANNNNTTC | 1 cut(s) | 48 |
| MseI | TTAA | 2 cut(s) | 15, 170 |
| NcoI | CCATGG | 1 cut(s) | 77 |
| NdeII | GATC | 1 cut(s) | 239 |
| NlaIII | CATG | 1 cut(s) | 81 |
| NlaIV | GGNNCC | 1 cut(s) | 228 |
| PdmI | GAANNNNTTC | 1 cut(s) | 48 |
| PfeI | GAWTC | 1 cut(s) | 426 |
| PkrI | GCNGC | 1 cut(s) | 376 |
| PleI | GAGTC | 1 cut(s) | 40 |
| PpsI | GAGTC | 1 cut(s) | 40 |
| PspN4I | GGNNCC | 1 cut(s) | 228 |
| PstI | CTGCAG | 1 cut(s) | 130 |
| RsaI | GTAC | 2 cut(s) | 98, 246 |
| RsaNI | GTAC | 2 cut(s) | 97, 245 |
| SaqAI | TTAA | 2 cut(s) | 15, 170 |
| SatI | GCNGC | 1 cut(s) | 375 |
| Sau3AI | GATC | 1 cut(s) | 239 |
| SchI | GAGTC | 1 cut(s) | 41 |
| SduI | GDGCHC | 1 cut(s) | 273 |
| SetI | ASST | 8 cut(s) | 63, 90, 98, 108, 217, 283, 296, 384 |
| SfaNI | GCATC | 1 cut(s) | 351 |
| SfcI | CTRYAG | 2 cut(s) | 126, 145 |
| SmlI | CTYRAG | 2 cut(s) | 185, 233 |
| SmoI | CTYRAG | 2 cut(s) | 185, 233 |
| Sse9I | AATT | 2 cut(s) | 191, 197 |
| SspMI | CTAG | 2 cut(s) | 117, 203 |
| StyI | CCWWGG | 3 cut(s) | 77, 303, 344 |
| TaaI | ACNGT | 3 cut(s) | 25, 149, 352 |
| TasI | AATT | 2 cut(s) | 191, 197 |
| TfiI | GAWTC | 1 cut(s) | 426 |
| Tru1I | TTAA | 2 cut(s) | 15, 170 |
| Tru9I | TTAA | 2 cut(s) | 15, 170 |
| TscAI | CASTG | 1 cut(s) | 154 |
| TseI | GCWGC | 1 cut(s) | 374 |
| TspDTI | ATGAA | 2 cut(s) | 225, 414 |
| TspRI | CASTG | 1 cut(s) | 154 |
| XapI | RAATTY | 1 cut(s) | 197 |
| XmnI | GAANNNNTTC | 1 cut(s) | 48 |
| XspI | CTAG | 2 cut(s) | 117, 203 |