Regulatory subunit of the dimeric E1 enzyme. E1 activates RUB1 NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AbsI | CCTCGAGG | 1 cut(s) | 254 |
| AccB1I | GGYRCC | 1 cut(s) | 247 |
| AclWI | GGATC | 1 cut(s) | 268 |
| AcsI | RAATTY | 1 cut(s) | 218 |
| AfaI | GTAC | 2 cut(s) | 119, 267 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 257 |
| AflIII | ACRYGT | 1 cut(s) | 291 |
| AgsI | TTSAA | 2 cut(s) | 91, 285 |
| AluBI | AGCT | 2 cut(s) | 109, 127 |
| AluI | AGCT | 2 cut(s) | 109, 127 |
| AlwI | GGATC | 1 cut(s) | 268 |
| Ama87I | CYCGRG | 1 cut(s) | 254 |
| AoxI | GGCC | 1 cut(s) | 101 |
| ApoI | RAATTY | 1 cut(s) | 218 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 69 |
| AsuHPI | GGTGA | 1 cut(s) | 254 |
| AvaI | CYCGRG | 1 cut(s) | 254 |
| BanI | GGYRCC | 1 cut(s) | 247 |
| BfaI | CTAG | 2 cut(s) | 138, 224 |
| BfmI | CTRYAG | 2 cut(s) | 147, 166 |
| BmeT110I | CYCGRG | 1 cut(s) | 254 |
| BmiI | GGNNCC | 1 cut(s) | 249 |
| Bpu10I | CCTNAGC | 1 cut(s) | 105 |
| BpuEI | CTTGAG | 1 cut(s) | 227 |
| BsaJI | CCNNGG | 1 cut(s) | 98 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 257 |
| BseDI | CCNNGG | 1 cut(s) | 98 |
| BseGI | GGATG | 2 cut(s) | 82, 147 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 257 |
| BseMII | CTCAG | 1 cut(s) | 96 |
| BsgI | GTGCAG | 1 cut(s) | 53 |
| BshFI | GGCC | 1 cut(s) | 103 |
| BshNI | GGYRCC | 1 cut(s) | 247 |
| BsiHKCI | CYCGRG | 1 cut(s) | 254 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 257 |
| BsnI | GGCC | 1 cut(s) | 103 |
| BsoBI | CYCGRG | 1 cut(s) | 254 |
| Bsp143I | GATC | 1 cut(s) | 260 |
| Bsp19I | CCATGG | 1 cut(s) | 98 |
| BspANI | GGCC | 1 cut(s) | 103 |
| BspCNI | CTCAG | 1 cut(s) | 97 |
| BspLI | GGNNCC | 1 cut(s) | 249 |
| BspMAI | CTGCAG | 1 cut(s) | 151 |
| BspPI | GGATC | 1 cut(s) | 268 |
| BspT107I | GGYRCC | 1 cut(s) | 247 |
| BssECI | CCNNGG | 1 cut(s) | 98 |
| BssMI | GATC | 1 cut(s) | 260 |
| BssT1I | CCWWGG | 1 cut(s) | 98 |
| Bst4CI | ACNGT | 2 cut(s) | 46, 170 |
| BstDEI | CTNAG | 2 cut(s) | 105, 110 |
| BstDSI | CCRYGG | 1 cut(s) | 98 |
| BstF5I | GGATG | 2 cut(s) | 82, 147 |
| BstKTI | GATC | 1 cut(s) | 263 |
| BstMBI | GATC | 1 cut(s) | 260 |
| BstNSI | RCATGY | 1 cut(s) | 295 |
| BstSFI | CTRYAG | 2 cut(s) | 147, 166 |
| BsuRI | GGCC | 1 cut(s) | 103 |
| BtgI | CCRYGG | 1 cut(s) | 98 |
| BtsCI | GGATG | 2 cut(s) | 82, 147 |
| BtsIMutI | CAGTG | 1 cut(s) | 175 |
| Csp6I | GTAC | 2 cut(s) | 118, 266 |
| CviAII | CATG | 2 cut(s) | 99, 292 |
| CviJI | RGCY | 4 cut(s) | 61, 103, 109, 127 |
| CviKI_1 | RGCY | 4 cut(s) | 61, 103, 109, 127 |
| CviQI | GTAC | 2 cut(s) | 118, 266 |
| DdeI | CTNAG | 2 cut(s) | 105, 110 |
| DpnI | GATC | 1 cut(s) | 262 |
| DpnII | GATC | 1 cut(s) | 260 |
| DraI | TTTAAA | 1 cut(s) | 192 |
| Eco130I | CCWWGG | 1 cut(s) | 98 |
| Eco88I | CYCGRG | 1 cut(s) | 254 |
| EcoRI | GAATTC | 1 cut(s) | 218 |
| EcoT14I | CCWWGG | 1 cut(s) | 98 |
| ErhI | CCWWGG | 1 cut(s) | 98 |
| FaeI | CATG | 2 cut(s) | 102, 295 |
| FaiI | YATR | 5 cut(s) | 20, 42, 100, 275, 293 |
| FatI | CATG | 2 cut(s) | 98, 291 |
| FokI | GGATG | 2 cut(s) | 89, 154 |
| FspBI | CTAG | 2 cut(s) | 138, 224 |
| HaeIII | GGCC | 1 cut(s) | 103 |
| Hin1II | CATG | 2 cut(s) | 102, 295 |
| HinfI | GANTC | 1 cut(s) | 53 |
| HphI | GGTGA | 1 cut(s) | 254 |
| Hpy188III | TCNNGA | 1 cut(s) | 74 |
| HpyAV | CCTTC | 4 cut(s) | 16, 92, 108, 227 |
| HpyCH4III | ACNGT | 2 cut(s) | 46, 170 |
| HpyCH4V | TGCA | 2 cut(s) | 34, 149 |
| HpyF3I | CTNAG | 2 cut(s) | 105, 110 |
| Hsp92II | CATG | 2 cut(s) | 102, 295 |
| Kzo9I | GATC | 1 cut(s) | 260 |
| LpnPI | CCDG | 5 cut(s) | 13, 59, 75, 117, 282 |
| MaeI | CTAG | 2 cut(s) | 138, 224 |
| MalI | GATC | 1 cut(s) | 262 |
| MboI | GATC | 1 cut(s) | 260 |
| MboII | GAAGA | 1 cut(s) | 273 |
| MluCI | AATT | 2 cut(s) | 212, 218 |
| MlyI | GAGTC | 1 cut(s) | 62 |
| MnlI | CCTC | 4 cut(s) | 202, 238, 250, 263 |
| MroXI | GAANNNNTTC | 1 cut(s) | 69 |
| MseI | TTAA | 1 cut(s) | 191 |
| NcoI | CCATGG | 1 cut(s) | 98 |
| NdeII | GATC | 1 cut(s) | 260 |
| NlaIII | CATG | 2 cut(s) | 102, 295 |
| NlaIV | GGNNCC | 1 cut(s) | 249 |
| NspI | RCATGY | 1 cut(s) | 295 |
| PaeR7I | CTCGAG | 1 cut(s) | 254 |
| PciI | ACATGT | 1 cut(s) | 291 |
| PdmI | GAANNNNTTC | 1 cut(s) | 69 |
| PleI | GAGTC | 1 cut(s) | 61 |
| PpsI | GAGTC | 1 cut(s) | 61 |
| PscI | ACATGT | 1 cut(s) | 291 |
| PspN4I | GGNNCC | 1 cut(s) | 249 |
| PspXI | VCTCGAGB | 1 cut(s) | 254 |
| PstI | CTGCAG | 1 cut(s) | 151 |
| RsaI | GTAC | 2 cut(s) | 119, 267 |
| RsaNI | GTAC | 2 cut(s) | 118, 266 |
| SaqAI | TTAA | 1 cut(s) | 191 |
| Sau3AI | GATC | 1 cut(s) | 260 |
| SchI | GAGTC | 1 cut(s) | 62 |
| SetI | ASST | 5 cut(s) | 84, 111, 119, 129, 238 |
| SfcI | CTRYAG | 2 cut(s) | 147, 166 |
| Sfr274I | CTCGAG | 1 cut(s) | 254 |
| SlaI | CTCGAG | 1 cut(s) | 254 |
| SmlI | CTYRAG | 2 cut(s) | 206, 254 |
| SmoI | CTYRAG | 2 cut(s) | 206, 254 |
| Sse9I | AATT | 2 cut(s) | 212, 218 |
| SspMI | CTAG | 2 cut(s) | 138, 224 |
| StyI | CCWWGG | 1 cut(s) | 98 |
| TaaI | ACNGT | 2 cut(s) | 46, 170 |
| TaqI | TCGA | 1 cut(s) | 255 |
| TasI | AATT | 2 cut(s) | 212, 218 |
| Tru1I | TTAA | 1 cut(s) | 191 |
| Tru9I | TTAA | 1 cut(s) | 191 |
| TscAI | CASTG | 1 cut(s) | 175 |
| TspDTI | ATGAA | 1 cut(s) | 246 |
| TspRI | CASTG | 1 cut(s) | 175 |
| XapI | RAATTY | 1 cut(s) | 218 |
| XceI | RCATGY | 1 cut(s) | 295 |
| XhoI | CTCGAG | 1 cut(s) | 254 |
| XmnI | GAANNNNTTC | 1 cut(s) | 69 |
| XspI | CTAG | 2 cut(s) | 138, 224 |