Regulatory subunit of the dimeric E1 enzyme. E1 activates RUB1 NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AbsI | CCTCGAGG | 1 cut(s) | 125 |
| AccB1I | GGYRCC | 1 cut(s) | 118 |
| AclWI | GGATC | 1 cut(s) | 139 |
| AcsI | RAATTY | 1 cut(s) | 89 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 128, 196 |
| AgsI | TTSAA | 3 cut(s) | 16, 38, 156 |
| AluBI | AGCT | 1 cut(s) | 173 |
| AluI | AGCT | 1 cut(s) | 173 |
| Alw21I | GWGCWC | 1 cut(s) | 165 |
| AlwI | GGATC | 1 cut(s) | 139 |
| Ama87I | CYCGRG | 1 cut(s) | 125 |
| ApeKI | GCWGC | 1 cut(s) | 8 |
| ApoI | RAATTY | 1 cut(s) | 89 |
| AsuHPI | GGTGA | 1 cut(s) | 125 |
| AvaI | CYCGRG | 1 cut(s) | 125 |
| BanI | GGYRCC | 1 cut(s) | 118 |
| Bbv12I | GWGCWC | 1 cut(s) | 165 |
| BbvCI | CCTCAGC | 1 cut(s) | 201 |
| BbvI | GCAGC | 1 cut(s) | 20 |
| BfaI | CTAG | 2 cut(s) | 95, 215 |
| BglII | AGATCT | 1 cut(s) | 73 |
| BisI | GCNGC | 1 cut(s) | 9 |
| BlsI | GCNGC | 1 cut(s) | 10 |
| BmeT110I | CYCGRG | 1 cut(s) | 125 |
| BmiI | GGNNCC | 1 cut(s) | 120 |
| Bpu10I | CCTNAGC | 1 cut(s) | 201 |
| BsaJI | CCNNGG | 1 cut(s) | 195 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 128, 196 |
| Bse1I | ACTGG | 1 cut(s) | 55 |
| BseDI | CCNNGG | 1 cut(s) | 195 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 128, 196 |
| BseMII | CTCAG | 2 cut(s) | 150, 192 |
| BseNI | ACTGG | 1 cut(s) | 55 |
| BseXI | GCAGC | 1 cut(s) | 20 |
| BshNI | GGYRCC | 1 cut(s) | 118 |
| BsiHKAI | GWGCWC | 1 cut(s) | 165 |
| BsiHKCI | CYCGRG | 1 cut(s) | 125 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 128, 196 |
| BsoBI | CYCGRG | 1 cut(s) | 125 |
| Bsp1286I | GDGCHC | 1 cut(s) | 165 |
| Bsp143I | GATC | 2 cut(s) | 73, 131 |
| BspCNI | CTCAG | 2 cut(s) | 151, 193 |
| BspLI | GGNNCC | 1 cut(s) | 120 |
| BspPI | GGATC | 1 cut(s) | 139 |
| BspT107I | GGYRCC | 1 cut(s) | 118 |
| BsrI | ACTGG | 1 cut(s) | 55 |
| BssECI | CCNNGG | 1 cut(s) | 195 |
| BssMI | GATC | 2 cut(s) | 73, 131 |
| BssT1I | CCWWGG | 1 cut(s) | 195 |
| BstDEI | CTNAG | 2 cut(s) | 159, 201 |
| BstKTI | GATC | 2 cut(s) | 76, 134 |
| BstMBI | GATC | 2 cut(s) | 73, 131 |
| BstV1I | GCAGC | 1 cut(s) | 20 |
| BstX2I | RGATCY | 1 cut(s) | 73 |
| BstYI | RGATCY | 1 cut(s) | 73 |
| CviJI | RGCY | 4 cut(s) | 173, 194, 200, 206 |
| CviKI_1 | RGCY | 4 cut(s) | 173, 194, 200, 206 |
| DdeI | CTNAG | 2 cut(s) | 159, 201 |
| DpnI | GATC | 2 cut(s) | 75, 133 |
| DpnII | GATC | 2 cut(s) | 73, 131 |
| Eco130I | CCWWGG | 1 cut(s) | 195 |
| Eco88I | CYCGRG | 1 cut(s) | 125 |
| EcoRI | GAATTC | 1 cut(s) | 89 |
| EcoT14I | CCWWGG | 1 cut(s) | 195 |
| ErhI | CCWWGG | 1 cut(s) | 195 |
| FaiI | YATR | 4 cut(s) | 57, 66, 146, 168 |
| Fnu4HI | GCNGC | 1 cut(s) | 9 |
| Fsp4HI | GCNGC | 1 cut(s) | 9 |
| FspBI | CTAG | 2 cut(s) | 95, 215 |
| GluI | GCNGC | 1 cut(s) | 9 |
| HinfI | GANTC | 2 cut(s) | 38, 237 |
| HphI | GGTGA | 1 cut(s) | 125 |
| HpyAV | CCTTC | 1 cut(s) | 98 |
| HpyCH4V | TGCA | 1 cut(s) | 64 |
| HpyF3I | CTNAG | 2 cut(s) | 159, 201 |
| Kzo9I | GATC | 2 cut(s) | 73, 131 |
| LpnPI | CCDG | 2 cut(s) | 36, 153 |
| Lsp1109I | GCAGC | 1 cut(s) | 20 |
| MaeI | CTAG | 2 cut(s) | 95, 215 |
| MalI | GATC | 2 cut(s) | 75, 133 |
| MboI | GATC | 2 cut(s) | 73, 131 |
| MboII | GAAGA | 3 cut(s) | 28, 144, 232 |
| MflI | RGATCY | 1 cut(s) | 73 |
| MhlI | GDGCHC | 1 cut(s) | 165 |
| MluCI | AATT | 2 cut(s) | 68, 89 |
| MlyI | GAGTC | 1 cut(s) | 231 |
| MnlI | CCTC | 4 cut(s) | 109, 121, 134, 196 |
| NdeII | GATC | 2 cut(s) | 73, 131 |
| NlaIV | GGNNCC | 1 cut(s) | 120 |
| PaeR7I | CTCGAG | 1 cut(s) | 125 |
| PfeI | GAWTC | 1 cut(s) | 38 |
| PkrI | GCNGC | 1 cut(s) | 10 |
| PleI | GAGTC | 1 cut(s) | 231 |
| PpsI | GAGTC | 1 cut(s) | 231 |
| PspN4I | GGNNCC | 1 cut(s) | 120 |
| PspXI | VCTCGAGB | 1 cut(s) | 125 |
| PsuI | RGATCY | 1 cut(s) | 73 |
| SatI | GCNGC | 1 cut(s) | 9 |
| Sau3AI | GATC | 2 cut(s) | 73, 131 |
| SchI | GAGTC | 1 cut(s) | 231 |
| SduI | GDGCHC | 1 cut(s) | 165 |
| SetI | ASST | 2 cut(s) | 109, 175 |
| Sfr274I | CTCGAG | 1 cut(s) | 125 |
| SgeI | CNNG | 8 cut(s) | 63, 107, 137, 139, 152, 203, 208, 227 |
| SlaI | CTCGAG | 1 cut(s) | 125 |
| SmlI | CTYRAG | 1 cut(s) | 125 |
| SmoI | CTYRAG | 1 cut(s) | 125 |
| Sse9I | AATT | 2 cut(s) | 68, 89 |
| SspI | AATATT | 1 cut(s) | 34 |
| SspMI | CTAG | 2 cut(s) | 95, 215 |
| StyI | CCWWGG | 1 cut(s) | 195 |
| TaqI | TCGA | 1 cut(s) | 126 |
| TasI | AATT | 2 cut(s) | 68, 89 |
| TfiI | GAWTC | 1 cut(s) | 38 |
| TseI | GCWGC | 1 cut(s) | 8 |
| TspDTI | ATGAA | 1 cut(s) | 117 |
| XapI | RAATTY | 1 cut(s) | 89 |
| XhoI | CTCGAG | 1 cut(s) | 125 |
| XspI | CTAG | 2 cut(s) | 95, 215 |