RchiOBHm_Chr1g0343391

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0003965
Physical Location & Seq
Forward (+)
35481748 .. 35487827
6080 bp
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UTR
Exon/CDS
Intron
PRQ56992

Sequence Viewer

Length: 213 bp
ATGAAGGTGTGGGATAGATCTATTAGAGTTTTGGTGAAAATTGGGGGCTTCGAAGCCATTCACAAGCAGGAAAAAATCATTGAAGCTTCCTATGGAGTCATGATTGCTCGGGTTGATCTTGTAGTTGAAATTCCACTCCAAGAGAAAATAACTCAAGTGTGTGGGCAGCTAAACAATCAAGTATTTGTAGCTTTCAACTACTTGAATACCTAA

Protein Analysis

70

Amino Acids

8.01

Weight (kDa)

7.89

Isoelectric Point (pI)

29.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 5 - 61 6.6e-08 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 129
AgsI TTSAA 4 cut(s) 83, 128, 196, 205
AluBI AGCT 3 cut(s) 86, 169, 191
AluI AGCT 3 cut(s) 86, 169, 191
Ama87I CYCGRG 1 cut(s) 108
ApeKI GCWGC 1 cut(s) 166
ApoI RAATTY 1 cut(s) 129
Asp700I GAANNNNTTC 1 cut(s) 57
AsuHPI GGTGA 1 cut(s) 46
AsuII TTCGAA 1 cut(s) 51
AvaI CYCGRG 1 cut(s) 108
BbvI GCAGC 1 cut(s) 178
BglII AGATCT 1 cut(s) 17
BisI GCNGC 1 cut(s) 167
BlsI GCNGC 1 cut(s) 168
BmeT110I CYCGRG 1 cut(s) 108
Bpu14I TTCGAA 1 cut(s) 51
BpuEI CTTGAG 1 cut(s) 138
BseXI GCAGC 1 cut(s) 178
BsiHKCI CYCGRG 1 cut(s) 108
BsoBI CYCGRG 1 cut(s) 108
Bsp119I TTCGAA 1 cut(s) 51
Bsp143I GATC 2 cut(s) 17, 115
BspHI TCATGA 1 cut(s) 99
BspT104I TTCGAA 1 cut(s) 51
BssMI GATC 2 cut(s) 17, 115
BstBI TTCGAA 1 cut(s) 51
BstKTI GATC 2 cut(s) 20, 118
BstMBI GATC 2 cut(s) 17, 115
BstV1I GCAGC 1 cut(s) 178
BstX2I RGATCY 1 cut(s) 17
BstYI RGATCY 1 cut(s) 17
CciI TCATGA 1 cut(s) 99
CviAII CATG 1 cut(s) 100
CviJI RGCY 5 cut(s) 48, 56, 86, 169, 191
CviKI_1 RGCY 5 cut(s) 48, 56, 86, 169, 191
DpnI GATC 2 cut(s) 19, 117
DpnII GATC 2 cut(s) 17, 115
Eco88I CYCGRG 1 cut(s) 108
FaeI CATG 1 cut(s) 103
FaiI YATR 2 cut(s) 93, 101
FatI CATG 1 cut(s) 99
Fnu4HI GCNGC 1 cut(s) 167
Fsp4HI GCNGC 1 cut(s) 167
GluI GCNGC 1 cut(s) 167
Hin1II CATG 1 cut(s) 103
HindIII AAGCTT 1 cut(s) 84
HinfI GANTC 1 cut(s) 96
HphI GGTGA 1 cut(s) 46
Hpy188III TCNNGA 1 cut(s) 100
Hsp92II CATG 1 cut(s) 103
Kzo9I GATC 2 cut(s) 17, 115
LpnPI CCDG 1 cut(s) 53
Lsp1109I GCAGC 1 cut(s) 178
MalI GATC 2 cut(s) 19, 117
MboI GATC 2 cut(s) 17, 115
MflI RGATCY 1 cut(s) 17
MluCI AATT 2 cut(s) 39, 129
MlyI GAGTC 1 cut(s) 105
MroXI GAANNNNTTC 1 cut(s) 57
NdeII GATC 2 cut(s) 17, 115
NlaIII CATG 1 cut(s) 103
NspV TTCGAA 1 cut(s) 51
PagI TCATGA 1 cut(s) 99
PdmI GAANNNNTTC 1 cut(s) 57
PkrI GCNGC 1 cut(s) 168
PleI GAGTC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 104
PsuI RGATCY 1 cut(s) 17
SatI GCNGC 1 cut(s) 167
Sau3AI GATC 2 cut(s) 17, 115
SchI GAGTC 1 cut(s) 105
SetI ASST 5 cut(s) 9, 88, 171, 193, 212
SfuI TTCGAA 1 cut(s) 51
SgeI CNNG 9 cut(s) 76, 80, 112, 120, 122, 131, 152, 167, 191
SmlI CTYRAG 1 cut(s) 153
SmoI CTYRAG 1 cut(s) 153
Sse9I AATT 2 cut(s) 39, 129
TaqI TCGA 1 cut(s) 51
TasI AATT 2 cut(s) 39, 129
TseI GCWGC 1 cut(s) 166
TspDTI ATGAA 1 cut(s) 17
XapI RAATTY 1 cut(s) 129
XmnI GAANNNNTTC 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.