Rh1CG169600

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0003965
Physical Location & Seq
Forward (+)
37212837 .. 37216877
4041 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG169600.1

Sequence Viewer

Length: 444 bp
ATGAAATATGATAGATTTGAAAAAACAAGATCTATTAGAGTTTTGGTGAAAATTGGGGGCTTCGAAGCCATTCACAAGCAGGAAAAAATCATTGAAGCTTCCTATGGAGTCATGATTGCTCGGGTTGATCTTGGAGTTGAAATTCCACTCCAAGAGAAAATAACTCAAGTGTGTGGGCAGCTAAACAATCAAGTATTTGTAGCTTCTCAACTACTTGAATACCCAACCCCAACACATGCCGAGTGTGATGAACCAGTTTCAATGAAACCGTGTGCTTCCCCATCATGGACTACACAAAATCAAGAAGAGCTAGATTGCAAAACACTATCAGAGGTTTTAAAGAGCTTGGAAAAGAAGGTGCCTAACTTGAAAATTTTCACCTATGAACGATTGGATCAGTTGAAAAGAGCTGCCATCTTCACTGAATGTGAAGTGAGAATGTGA

Protein Analysis

147

Amino Acids

16.85

Weight (kDa)

6.16

Isoelectric Point (pI)

41.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 7 - 82 4.6e-11 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 358
AclWI GGATC 1 cut(s) 402
AcsI RAATTY 2 cut(s) 141, 372
AfiI CCNNNNNNNGG 1 cut(s) 285
AgsI TTSAA 7 cut(s) 20, 95, 140, 218, 261, 370, 403
AluBI AGCT 6 cut(s) 98, 181, 203, 310, 345, 410
AluI AGCT 6 cut(s) 98, 181, 203, 310, 345, 410
AlwI GGATC 1 cut(s) 402
Ama87I CYCGRG 1 cut(s) 120
ApeKI GCWGC 2 cut(s) 178, 410
ApoI RAATTY 2 cut(s) 141, 372
Asp700I GAANNNNTTC 2 cut(s) 69, 374
AsuHPI GGTGA 2 cut(s) 58, 370
AsuII TTCGAA 1 cut(s) 63
AvaI CYCGRG 1 cut(s) 120
BanI GGYRCC 1 cut(s) 358
BbvI GCAGC 2 cut(s) 190, 397
BccI CCATC 2 cut(s) 289, 422
BfaI CTAG 1 cut(s) 311
BglII AGATCT 1 cut(s) 29
BisI GCNGC 2 cut(s) 179, 411
BlsI GCNGC 2 cut(s) 180, 412
BmeT110I CYCGRG 1 cut(s) 120
BmiI GGNNCC 1 cut(s) 360
Bpu14I TTCGAA 1 cut(s) 63
BpuEI CTTGAG 1 cut(s) 150
Bsc4I CCNNNNNNNGG 1 cut(s) 285
Bse1I ACTGG 1 cut(s) 254
BseLI CCNNNNNNNGG 1 cut(s) 285
BseNI ACTGG 1 cut(s) 254
BseXI GCAGC 2 cut(s) 190, 397
BshNI GGYRCC 1 cut(s) 358
BsiHKCI CYCGRG 1 cut(s) 120
BslI CCNNNNNNNGG 1 cut(s) 285
BsoBI CYCGRG 1 cut(s) 120
Bsp119I TTCGAA 1 cut(s) 63
Bsp143I GATC 3 cut(s) 29, 127, 394
BspHI TCATGA 1 cut(s) 111
BspLI GGNNCC 1 cut(s) 360
BspPI GGATC 1 cut(s) 402
BspQI GCTCTTC 1 cut(s) 300
BspT104I TTCGAA 1 cut(s) 63
BspT107I GGYRCC 1 cut(s) 358
BsrI ACTGG 1 cut(s) 254
BssMI GATC 3 cut(s) 29, 127, 394
Bst4CI ACNGT 1 cut(s) 270
Bst6I CTCTTC 1 cut(s) 300
BstBI TTCGAA 1 cut(s) 63
BstKTI GATC 3 cut(s) 32, 130, 397
BstMBI GATC 3 cut(s) 29, 127, 394
BstNSI RCATGY 1 cut(s) 239
BstV1I GCAGC 2 cut(s) 190, 397
BstX2I RGATCY 1 cut(s) 29
BstYI RGATCY 1 cut(s) 29
BtsIMutI CAGTG 1 cut(s) 420
CciI TCATGA 1 cut(s) 111
CviAII CATG 3 cut(s) 112, 236, 285
CviJI RGCY 8 cut(s) 60, 68, 98, 181, 203, 310, 345, 410
CviKI_1 RGCY 8 cut(s) 60, 68, 98, 181, 203, 310, 345, 410
DpnI GATC 3 cut(s) 31, 129, 396
DpnII GATC 3 cut(s) 29, 127, 394
DraI TTTAAA 1 cut(s) 339
Eam1104I CTCTTC 1 cut(s) 300
EarI CTCTTC 1 cut(s) 300
Eco88I CYCGRG 1 cut(s) 120
FaeI CATG 3 cut(s) 115, 239, 288
FaiI YATR 6 cut(s) 9, 105, 113, 237, 286, 384
FatI CATG 3 cut(s) 111, 235, 284
Fnu4HI GCNGC 2 cut(s) 179, 411
Fsp4HI GCNGC 2 cut(s) 179, 411
FspBI CTAG 1 cut(s) 311
GluI GCNGC 2 cut(s) 179, 411
Hin1II CATG 3 cut(s) 115, 239, 288
HindIII AAGCTT 1 cut(s) 96
HinfI GANTC 1 cut(s) 108
HphI GGTGA 2 cut(s) 58, 370
Hpy188I TCNGA 1 cut(s) 331
Hpy188III TCNNGA 2 cut(s) 112, 302
HpyAV CCTTC 1 cut(s) 349
HpyCH4III ACNGT 1 cut(s) 270
HpyCH4V TGCA 1 cut(s) 318
Hsp92II CATG 3 cut(s) 115, 239, 288
Kzo9I GATC 3 cut(s) 29, 127, 394
LguI GCTCTTC 1 cut(s) 300
LpnPI CCDG 2 cut(s) 65, 267
Lsp1109I GCAGC 2 cut(s) 190, 397
MaeI CTAG 1 cut(s) 311
MalI GATC 3 cut(s) 31, 129, 396
MboI GATC 3 cut(s) 29, 127, 394
MboII GAAGA 2 cut(s) 317, 409
MflI RGATCY 1 cut(s) 29
MluCI AATT 3 cut(s) 51, 141, 372
MlyI GAGTC 1 cut(s) 117
MnlI CCTC 1 cut(s) 325
MroXI GAANNNNTTC 2 cut(s) 69, 374
MseI TTAA 1 cut(s) 338
NdeII GATC 3 cut(s) 29, 127, 394
NlaIII CATG 3 cut(s) 115, 239, 288
NlaIV GGNNCC 1 cut(s) 360
NmeAIII GCCGAG 1 cut(s) 265
NspI RCATGY 1 cut(s) 239
NspV TTCGAA 1 cut(s) 63
PagI TCATGA 1 cut(s) 111
PciSI GCTCTTC 1 cut(s) 300
PdmI GAANNNNTTC 2 cut(s) 69, 374
PkrI GCNGC 2 cut(s) 180, 412
PleI GAGTC 1 cut(s) 116
PpsI GAGTC 1 cut(s) 116
PspN4I GGNNCC 1 cut(s) 360
PsuI RGATCY 1 cut(s) 29
SapI GCTCTTC 1 cut(s) 300
SaqAI TTAA 1 cut(s) 338
SatI GCNGC 2 cut(s) 179, 411
Sau3AI GATC 3 cut(s) 29, 127, 394
SchI GAGTC 1 cut(s) 117
SetI ASST 9 cut(s) 100, 183, 205, 312, 336, 347, 360, 383, 412
SfuI TTCGAA 1 cut(s) 63
SmlI CTYRAG 1 cut(s) 165
SmoI CTYRAG 1 cut(s) 165
Sse9I AATT 3 cut(s) 51, 141, 372
SspMI CTAG 1 cut(s) 311
TaaI ACNGT 1 cut(s) 270
TaqI TCGA 1 cut(s) 63
TasI AATT 3 cut(s) 51, 141, 372
Tru1I TTAA 1 cut(s) 338
Tru9I TTAA 1 cut(s) 338
TscAI CASTG 1 cut(s) 427
TseI GCWGC 2 cut(s) 178, 410
TspDTI ATGAA 4 cut(s) 17, 264, 278, 399
TspRI CASTG 1 cut(s) 427
XapI RAATTY 2 cut(s) 141, 372
XceI RCATGY 1 cut(s) 239
XmnI GAANNNNTTC 2 cut(s) 69, 374
XspI CTAG 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.