Rorug05G0357900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003965
Physical Location & Seq
Forward (+)
45342606 .. 45345267
2662 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0357900.1

Sequence Viewer

Length: 1653 bp
ATGAGTGGTTGCATGCTGTTCCTACAATTATTTTATTTTGATTGTGTTGGTTATAGTGCGTCCATGGTGTCGAAATCAGTTCACCCTGTTGTTGCATGGACCAAGAAGACGTGTCGGAGCATGATTGAGTGGATAAGTTCACAAGGGGGAAGCCACGACGGTTGTGTTTATATATCCAACAGTGTTGGTACGAAAATATGGGAGAATCTGGAATCAGACTATGGTTGCCAACTCGCGCGTGAAAGTGTGCCTGAAGACGTGTTGAAGGCCTTGTTTCAAATATACGGGGTGCAAAATGAAGTTTGGTTGGTGTCTTCAAAGATGGGATGTATGGAGGCCAACATTGAGAGGGTGAGGAAGATAATATTTGAAATTGGAAAAATATTGCGTGAAATGAATGTTGGTGTTAGAGGACAGCTTCAGTCGTACACAATGAAAATAGGAAATGGAAATGAGTTTGGAAGTGTCGATGATGGGAGTGTAATTGGTATTGATACCGAACCGTGGCAGAAACGTCCACTGTCCTCATATTTTGATGGAGTATTATGGCACCAAGGAGCGTACGATGAAGCAGCAGAAGAGGGTGATAGGTATGTGGAGGACACAGGTGCGGATGTTGGACTGAAACCAGGTCCTGTCTCCATAGATGATGGTAATGTTGGAAAGGAGGAAGATAGGTTCTCATTGAAGGGAAATCTTTCACGCACTGAGTGGACATTGTTGAAGTTTCTGTTTGAGAAGCCTGCGAGAGGACAAGATAAGTGTAGGGATGAAGTTGCTCGGTTTGGAGAATGGATAATGTCCACAGCTGAATTTGATTGTTTGTGTCTGGGGAATAAAATTTCCAGCAAGGTGATAAACATATCGGCAGCATACTTGTTTGAAAAGGAGTCCGACTCATGGTTCCTCCCAACCAATTTTGGGGATCAAGCTTGTTATAAAAACCAGTCATCGAAAGTGGAGACATCGTTGGCATCAATCATATCGGAATGTGGCCTGCATAGGTTTAATCGGCGGTTGAAGTGGTGTTCTAAGATATTCATCCCCATCCATGATAAAATGTCAGAACATTGGTTTTTGGTTGTTACGAGAGTTTCTGAAAAAGTGGTGGAGATTTGGGATAGCAATACTGATATTGTTTCCTTTCCTAGAAGGCATCAGATGGCACGAGATGTGATATTGCTCATGCAAAAAGTTTTTGCAAGAGATATGAAAAAACCCAGTGATGTTTATTACCAATTTTCGTCATTTGAAACTAAGCAACCAGATTGTAACCCAAGTAACAACATTGATTGGAAAAGTGGCGTCTACCTGATAAGGCACATGCAGTTGTACGGTCAACCATGGTTTGAAGGGTTTCAGTCCAATAAGTATAGGAGTGCGCTAGCCTTAGGGATTGTGAACAATCCAAAGAATGAAGCACTGAAGGTGGTGACTGAGTCGGCTGATGTATCACACAGTCGTTCCAAAAACACGTTAAATGGCAGAGATAATTGTACTGGACCATCGGGAGATAACATGGAAGCTGAAACGGATTCCGAACTACCCAACTCTGACAATAATGTGAATATCCCAAGTGGAAGGAAGTTCCGCTCTCACCGGAAGCGAAAGGACCTAGGAGCATCATTGACACCACTTTTCCCATATTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

550

Amino Acids

62.36

Weight (kDa)

6.13

Isoelectric Point (pI)

41.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C48 PF02902 314 - 445 6.2e-08 Ulp1 protease family, C-terminal catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 939
AccB1I GGYRCC 1 cut(s) 549
AccBSI CCGCTC 1 cut(s) 1593
AccI GTMKAC 1 cut(s) 1308
AccII CGCG 2 cut(s) 236, 238
AciI CCGC 3 cut(s) 611, 1015, 1591
AclWI GGATC 1 cut(s) 933
AcsI RAATTY 2 cut(s) 812, 840
AcuI CTGAAG 3 cut(s) 273, 404, 1445
AcyI GRCGYC 1 cut(s) 1305
AdeI CACNNNGTG 1 cut(s) 711
AfaI GTAC 5 cut(s) 190, 428, 563, 1334, 1498
AfiI CCNNNNNNNGG 4 cut(s) 504, 749, 900, 921
AflIII ACRYGT 3 cut(s) 110, 258, 1473
AjiI CACGTC 2 cut(s) 111, 259
AjnI CCWGG 1 cut(s) 628
AjuI GAANNNNNNNTTGG 2 cut(s) 384, 416
AloI GAACNNNNNNTCC 2 cut(s) 662, 694
AluBI AGCT 4 cut(s) 418, 809, 932, 1526
AluI AGCT 4 cut(s) 418, 809, 932, 1526
Alw26I GTCTC 2 cut(s) 643, 956
AlwI GGATC 1 cut(s) 933
AlwNI CAGNNNCTG 1 cut(s) 635
AoxI GGCC 3 cut(s) 267, 336, 994
ApeKI GCWGC 2 cut(s) 572, 869
ApoI RAATTY 2 cut(s) 812, 840
Asp700I GAANNNNTTC 2 cut(s) 697, 1356
AspA2I CCTAGG 1 cut(s) 1615
AspLEI GCGC 2 cut(s) 238, 1384
AspS9I GGNCC 4 cut(s) 99, 632, 1502, 1612
AsuHPI GGTGA 6 cut(s) 74, 364, 596, 865, 1444, 1589
AsuNHI GCTAGC 1 cut(s) 1384
AvaII GGWCC 4 cut(s) 99, 632, 1502, 1612
AvrII CCTAGG 1 cut(s) 1615
AxyI CCTNAGG 1 cut(s) 1390
BanI GGYRCC 1 cut(s) 549
BauI CACGAG 1 cut(s) 1167
BbsI GAAGAC 3 cut(s) 113, 261, 306
BbvI GCAGC 2 cut(s) 584, 881
BccI CCATC 7 cut(s) 316, 467, 530, 644, 1055, 1156, 1513
BciT130I CCWGG 1 cut(s) 630
BcoDI GTCTC 2 cut(s) 643, 956
BfaI CTAG 3 cut(s) 1149, 1385, 1616
BisI GCNGC 2 cut(s) 573, 870
BlnI CCTAGG 1 cut(s) 1615
BlsI GCNGC 2 cut(s) 574, 871
Bme1390I CCNGG 1 cut(s) 630
Bme18I GGWCC 4 cut(s) 99, 632, 1502, 1612
BmgBI CACGTC 2 cut(s) 111, 259
BmgT120I GGNCC 4 cut(s) 99, 632, 1502, 1612
BmiI GGNNCC 2 cut(s) 551, 905
BmrFI CCNGG 1 cut(s) 630
BmrI ACTGGG 1 cut(s) 1215
BmsI GCATC 3 cut(s) 983, 1165, 1631
BmtI GCTAGC 1 cut(s) 1388
BmuI ACTGGG 1 cut(s) 1215
BpiI GAAGAC 3 cut(s) 113, 261, 306
BplI GAGNNNNNCTC 2 cut(s) 881, 913
BsaBI GATNNNNATC 1 cut(s) 1040
BsaHI GRCGYC 1 cut(s) 1305
BsaJI CCNNGG 5 cut(s) 63, 503, 553, 1343, 1615
BsaWI WCCGGW 1 cut(s) 1599
BsaXI ACNNNNNCTCC 2 cut(s) 953, 983
Bsc4I CCNNNNNNNGG 4 cut(s) 504, 749, 900, 921
Bse1I ACTGG 3 cut(s) 946, 1221, 1504
Bse21I CCTNAGG 1 cut(s) 1390
Bse8I GATNNNNATC 1 cut(s) 1040
BseBI CCWGG 1 cut(s) 630
BseDI CCNNGG 5 cut(s) 63, 503, 553, 1343, 1615
BseGI GGATG 5 cut(s) 332, 619, 775, 1041, 1047
BseJI GATNNNNATC 1 cut(s) 1040
BseLI CCNNNNNNNGG 4 cut(s) 504, 749, 900, 921
BseMII CTCAG 2 cut(s) 699, 1428
BseNI ACTGG 3 cut(s) 946, 1221, 1504
BseXI GCAGC 2 cut(s) 584, 881
Bsh1236I CGCG 2 cut(s) 236, 238
BshFI GGCC 3 cut(s) 269, 338, 996
BshNI GGYRCC 1 cut(s) 549
BsiSI CCGG 1 cut(s) 1600
BsiWI CGTACG 1 cut(s) 561
BslI CCNNNNNNNGG 4 cut(s) 504, 749, 900, 921
BsmAI GTCTC 2 cut(s) 643, 956
BsnI GGCC 3 cut(s) 269, 338, 996
Bsp143I GATC 1 cut(s) 925
Bsp19I CCATGG 2 cut(s) 63, 1343
BspACI CCGC 3 cut(s) 611, 1015, 1591
BspANI GGCC 3 cut(s) 269, 338, 996
BspCNI CTCAG 2 cut(s) 700, 1429
BspFNI CGCG 2 cut(s) 236, 238
BspLI GGNNCC 2 cut(s) 551, 905
BspOI GCTAGC 1 cut(s) 1388
BspPI GGATC 1 cut(s) 933
BspT107I GGYRCC 1 cut(s) 549
BsrBI CCGCTC 1 cut(s) 1593
BsrI ACTGG 3 cut(s) 946, 1221, 1504
BssECI CCNNGG 5 cut(s) 63, 503, 553, 1343, 1615
BssMI GATC 1 cut(s) 925
BssNI GRCGYC 1 cut(s) 1305
BssSI CACGAG 1 cut(s) 1167
BssT1I CCWWGG 4 cut(s) 63, 553, 1343, 1615
Bst2BI CACGAG 1 cut(s) 1167
Bst2UI CCWGG 1 cut(s) 630
Bst4CI ACNGT 6 cut(s) 161, 182, 504, 522, 1337, 1460
Bst6I CTCTTC 1 cut(s) 573
BstACI GRCGYC 1 cut(s) 1305
BstC8I GCNNGC 4 cut(s) 14, 744, 998, 1386
BstDEI CTNAG 5 cut(s) 708, 1032, 1257, 1390, 1437
BstDSI CCRYGG 3 cut(s) 63, 503, 1343
BstENI CCTNNNNNAGG 1 cut(s) 747
BstF5I GGATG 5 cut(s) 332, 619, 775, 1041, 1047
BstFNI CGCG 2 cut(s) 236, 238
BstHHI GCGC 2 cut(s) 238, 1384
BstKTI GATC 1 cut(s) 928
BstMAI GTCTC 2 cut(s) 643, 956
BstMBI GATC 1 cut(s) 925
BstNI CCWGG 1 cut(s) 630
BstNSI RCATGY 2 cut(s) 16, 1327
BstSCI CCNGG 1 cut(s) 628
BstUI CGCG 2 cut(s) 236, 238
BstV1I GCAGC 2 cut(s) 584, 881
BstV2I GAAGAC 3 cut(s) 113, 261, 306
Bsu36I CCTNAGG 1 cut(s) 1390
BsuRI GGCC 3 cut(s) 269, 338, 996
BtgI CCRYGG 3 cut(s) 63, 503, 1343
BtrI CACGTC 2 cut(s) 111, 259
BtsCI GGATG 5 cut(s) 332, 619, 775, 1041, 1047
BtsIMutI CAGTG 5 cut(s) 187, 518, 705, 1228, 1421
Cac8I GCNNGC 4 cut(s) 14, 744, 998, 1386
CaiI CAGNNNCTG 1 cut(s) 635
CfoI GCGC 2 cut(s) 238, 1384
Cfr13I GGNCC 4 cut(s) 99, 632, 1502, 1612
CseI GACGC 2 cut(s) 48, 1294
CsiI ACCWGGT 1 cut(s) 628
Csp6I GTAC 5 cut(s) 189, 427, 562, 1333, 1497
CspCI CAANNNNNGTGG 2 cut(s) 143, 178
CviQI GTAC 5 cut(s) 189, 427, 562, 1333, 1497
DdeI CTNAG 5 cut(s) 708, 1032, 1257, 1390, 1437
DpnI GATC 1 cut(s) 927
DpnII GATC 1 cut(s) 925
DraIII CACNNNGTG 1 cut(s) 711
Eam1104I CTCTTC 1 cut(s) 573
EarI CTCTTC 1 cut(s) 573
Eco130I CCWWGG 4 cut(s) 63, 553, 1343, 1615
Eco147I AGGCCT 1 cut(s) 269
Eco47I GGWCC 4 cut(s) 99, 632, 1502, 1612
Eco57I CTGAAG 3 cut(s) 273, 404, 1445
Eco81I CCTNAGG 1 cut(s) 1390
EcoNI CCTNNNNNAGG 1 cut(s) 747
EcoO109I RGGNCCY 2 cut(s) 632, 1612
EcoRII CCWGG 1 cut(s) 628
EcoT14I CCWWGG 4 cut(s) 63, 553, 1343, 1615
ErhI CCWWGG 4 cut(s) 63, 553, 1343, 1615
FblI GTMKAC 1 cut(s) 1308
Fnu4HI GCNGC 2 cut(s) 573, 870
FokI GGATG 5 cut(s) 339, 626, 782, 1028, 1034
Fsp4HI GCNGC 2 cut(s) 573, 870
FspBI CTAG 3 cut(s) 1149, 1385, 1616
GlaI GCGC 2 cut(s) 237, 1383
GluI GCNGC 2 cut(s) 573, 870
HaeIII GGCC 3 cut(s) 269, 338, 996
HapII CCGG 1 cut(s) 1600
HgaI GACGC 2 cut(s) 48, 1294
HhaI GCGC 2 cut(s) 238, 1384
Hin1I GRCGYC 1 cut(s) 1305
Hin6I GCGC 2 cut(s) 236, 1382
HinP1I GCGC 2 cut(s) 236, 1382
HincII GTYRAC 1 cut(s) 1340
HindII GTYRAC 1 cut(s) 1340
HindIII AAGCTT 1 cut(s) 930
HinfI GANTC 6 cut(s) 205, 212, 890, 896, 1439, 1535
HpaII CCGG 1 cut(s) 1600
HphI GGTGA 6 cut(s) 74, 364, 596, 865, 1444, 1589
Hpy166II GTNNAC 9 cut(s) 82, 140, 429, 518, 714, 804, 1309, 1340, 1402
Hpy188I TCNGA 9 cut(s) 117, 217, 895, 988, 1066, 1099, 1161, 1540, 1555
Hpy188III TCNNGA 2 cut(s) 209, 1509
Hpy8I GTNNAC 9 cut(s) 82, 140, 429, 518, 714, 804, 1309, 1340, 1402
Hpy99I CGWCG 1 cut(s) 161
HpyAV CCTTC 6 cut(s) 259, 682, 1146, 1346, 1420, 1575
HpyCH4III ACNGT 6 cut(s) 161, 182, 504, 522, 1337, 1460
HpyCH4IV ACGT 4 cut(s) 110, 258, 514, 1475
HpyCH4V TGCA 7 cut(s) 12, 95, 292, 1000, 1189, 1202, 1327
HpyF3I CTNAG 5 cut(s) 708, 1032, 1257, 1390, 1437
HpySE526I ACGT 4 cut(s) 110, 258, 514, 1475
Hsp92I GRCGYC 1 cut(s) 1305
HspAI GCGC 2 cut(s) 236, 1382
Kzo9I GATC 1 cut(s) 925
LmnI GCTCC 3 cut(s) 117, 557, 1619
Lsp1109I GCAGC 2 cut(s) 584, 881
LweI GCATC 3 cut(s) 983, 1165, 1631
MabI ACCWGGT 1 cut(s) 628
MaeI CTAG 3 cut(s) 1149, 1385, 1616
MaeII ACGT 4 cut(s) 110, 258, 514, 1475
MaeIII GTNAC 4 cut(s) 1084, 1271, 1280, 1432
MalI GATC 1 cut(s) 927
MbiI CCGCTC 1 cut(s) 1593
MboI GATC 1 cut(s) 925
MboII GAAGA 6 cut(s) 118, 266, 306, 370, 590, 683
MluCI AATT 8 cut(s) 26, 372, 483, 812, 840, 916, 1238, 1492
MlyI GAGTC 3 cut(s) 890, 899, 1448
MmeI TCCRAC 5 cut(s) 95, 201, 598, 640, 918
MroXI GAANNNNTTC 2 cut(s) 697, 1356
MseI TTAA 2 cut(s) 1008, 1478
MspA1I CMGCKG 1 cut(s) 809
MspI CCGG 1 cut(s) 1600
MspR9I CCNGG 1 cut(s) 630
MvaI CCWGG 1 cut(s) 630
MvnI CGCG 2 cut(s) 236, 238
NcoI CCATGG 2 cut(s) 63, 1343
NdeII GATC 1 cut(s) 925
NheI GCTAGC 1 cut(s) 1384
NlaIV GGNNCC 2 cut(s) 551, 905
NmuCI GTSAC 1 cut(s) 1432
NspI RCATGY 2 cut(s) 16, 1327
PaeI GCATGC 1 cut(s) 16
PceI AGGCCT 1 cut(s) 269
PdmI GAANNNNTTC 2 cut(s) 697, 1356
PfeI GAWTC 3 cut(s) 205, 212, 1535
Pfl23II CGTACG 1 cut(s) 561
PflFI GACNNNGTC 1 cut(s) 1438
PkrI GCNGC 2 cut(s) 574, 871
PleI GAGTC 3 cut(s) 890, 898, 1447
PpsI GAGTC 3 cut(s) 890, 898, 1447
PpuMI RGGWCCY 2 cut(s) 632, 1612
PsiI TTATAA 1 cut(s) 939
Psp5II RGGWCCY 2 cut(s) 632, 1612
Psp6I CCWGG 1 cut(s) 628
PspGI CCWGG 1 cut(s) 628
PspLI CGTACG 1 cut(s) 561
PspN4I GGNNCC 2 cut(s) 551, 905
PspPI GGNCC 4 cut(s) 99, 632, 1502, 1612
PspPPI RGGWCCY 2 cut(s) 632, 1612
PstNI CAGNNNCTG 1 cut(s) 635
PsyI GACNNNGTC 1 cut(s) 1438
PvuII CAGCTG 1 cut(s) 809
RsaI GTAC 5 cut(s) 190, 428, 563, 1334, 1498
RsaNI GTAC 5 cut(s) 189, 427, 562, 1333, 1497
SaqAI TTAA 2 cut(s) 1008, 1478
SatI GCNGC 2 cut(s) 573, 870
Sau3AI GATC 1 cut(s) 925
Sau96I GGNCC 4 cut(s) 99, 632, 1502, 1612
SchI GAGTC 3 cut(s) 890, 899, 1448
ScrFI CCNGG 1 cut(s) 630
SexAI ACCWGGT 1 cut(s) 628
SfaNI GCATC 3 cut(s) 983, 1165, 1631
SinI GGWCC 4 cut(s) 99, 632, 1502, 1612
SphI GCATGC 1 cut(s) 16
Sse9I AATT 8 cut(s) 26, 372, 483, 812, 840, 916, 1238, 1492
SseBI AGGCCT 1 cut(s) 269
SsiI CCGC 3 cut(s) 611, 1015, 1591
SspI AATATT 2 cut(s) 366, 384
SspMI CTAG 3 cut(s) 1149, 1385, 1616
StuI AGGCCT 1 cut(s) 269
StyD4I CCNGG 1 cut(s) 628
StyI CCWWGG 4 cut(s) 63, 553, 1343, 1615
TaaI ACNGT 6 cut(s) 161, 182, 504, 522, 1337, 1460
TaiI ACGT 4 cut(s) 113, 261, 517, 1478
TaqI TCGA 3 cut(s) 71, 468, 953
TasI AATT 8 cut(s) 26, 372, 483, 812, 840, 916, 1238, 1492
TatI WGTACW 1 cut(s) 1496
TfiI GAWTC 3 cut(s) 205, 212, 1535
Tru1I TTAA 2 cut(s) 1008, 1478
Tru9I TTAA 2 cut(s) 1008, 1478
TscAI CASTG 5 cut(s) 187, 525, 712, 1228, 1428
TseFI GTSAC 1 cut(s) 1432
TseI GCWGC 2 cut(s) 572, 869
Tsp45I GTSAC 1 cut(s) 1432
TspDTI ATGAA 8 cut(s) 312, 410, 449, 582, 786, 1030, 1226, 1431
TspGWI ACGGA 1 cut(s) 1547
TspRI CASTG 5 cut(s) 187, 525, 712, 1228, 1428
Tth111I GACNNNGTC 1 cut(s) 1438
VpaK11BI GGWCC 4 cut(s) 99, 632, 1502, 1612
XagI CCTNNNNNAGG 1 cut(s) 747
XapI RAATTY 2 cut(s) 812, 840
XceI RCATGY 2 cut(s) 16, 1327
XmaJI CCTAGG 1 cut(s) 1615
XmiI GTMKAC 1 cut(s) 1308
XmnI GAANNNNTTC 2 cut(s) 697, 1356
XspI CTAG 3 cut(s) 1149, 1385, 1616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.