Rorug02G0268600

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003965
Physical Location & Seq
Reverse (-)
28384602 .. 28389718
5117 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0268600.1

Sequence Viewer

Length: 1494 bp
ATGGAAGTTGAGCTCATTGATCACCCATTCAACCCGAGCTACGACCTCTATGACTGTAGCTTTGAATCTGATTCTTCCTTCCAAACATCAGATTTATTTACTCCTTGTACCACCAATGTTGATCAAAACTCGGACCACGAGTGCCTCAACAGATTGCTCCAAATAGAATCACAGCTCATGGACATAGAATCGTCTATCAGCAGTGGAAGTGATGTAATCGGTCTTGATGGTGTTGTTGATAGTGAATTCGCTTCTTACATAAGTGAAGAAGCACCAGAAAATGTCCATGTTTCGACAGAAGGCAATTCCCTTTTGAAGGAGATTCAGATAGATTTGCTGGAAGAGAGCAGCTTAACTGAACTTTTATTCGCAGGAGCTGAAGCTGTTGAAGCACAGAACTGGGTTTTTGCTTCAAATACAATCGCAAAGCTCAACACTCTTCTATCCGGTAGCGAAAATGGAGACAACTCGTTCAACAAATTGGCCACGTTCTTCACTCAAGGCCTTCATTACAAGACCATTAACTCTATTGAAGTTCCCCATGAGCCAGCTGTTTCTAGAAACACAGACACAATGTCTTCATTTCAGATGCTCCAAGAGCTCTCTCCTTTTGTTAAGTTTGCGCATTTCACAGCCAACCAAGCGATTCTCGAGGCCACACAAGAGGATGAGGACATTCATGTCATTGACTTTGACATCATGGAGGGTAGCCAGTGGCCTTCATTGATGGTTGACCTTGCAATGAGGAAAGGTCGTCGCTGTGCTTCTCTCAAGGTAACCGCCATCACAGTTGACCAGCAAAGCGCCGCTCTTGCTCAACAAACTGGAAGAAGGCTTAAAGAGTTCTCAGAGTCTATCAATTTGTTATTTATTTTTGATCAGATGATGATGGCCACAGAAGAAGATTTTGCTAAAATCGAAGTTGTTGCTGATGCAGTAGTAGCCAATTGCATGATTCACCAGCTTCACATGCCTAACAGAAGTATCTCAATATTGAAGGCCTTCTTGGGTGGCATGAGCAAATTATCACCTAAACTCATTGTCCTAGTACAAGAAGAGTTGTTCAACTTTTCCAAAATGCCATCAATGGTCTTTGCAGAGTTCTTCTCGGAGGCTCTCCATCACTACAATTCGCTTTGCGACTCTCTTGTAACTAGTTTTAGTGGTGAGTATAAAATGGGGTTAAGACAGATAGAGAAAGAGGTTTTGGGGATCAAGATTTTAGAAAGTGTCGGGCAGTTCCCATGTGAGAAGGAGGATAGAATGTTGTGGGAAGATTGTTTTGCTTCGTTGAAGGGCTTCAAGTCTATCCCTTTAAGTTCTTGTAATGTTTCTCAAGCAAAATACTTGGTGAGTCTTTTCAATAGGGGATATTGGGTGCAGCAAGAGAAGGGCAGGTTAGCTTTGTGTTGGAAATCAAGACCTTTGACAACAGCTTCCATTTGGGTGCCCTTAACTTACAAAATCAAGGAAAACTATGTCAACCCCTTTTGA

Protein Analysis

497

Amino Acids

55.89

Weight (kDa)

4.66

Isoelectric Point (pI)

50.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 119 - 482 3.7e-77 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 680
Acc16I TGCGCA 1 cut(s) 624
Acc36I ACCTGC 1 cut(s) 1386
AccB1I GGYRCC 1 cut(s) 1447
AccBSI CCGCTC 1 cut(s) 809
AciI CCGC 2 cut(s) 780, 807
AclWI GGATC 1 cut(s) 1220
AcoI YGGCCR 2 cut(s) 483, 891
AcsI RAATTY 1 cut(s) 245
AcuI CTGAAG 1 cut(s) 399
AfaI GTAC 2 cut(s) 109, 1050
AfiI CCNNNNNNNGG 1 cut(s) 316
AhdI GACNNNNNGTC 1 cut(s) 574
AhlI ACTAGT 1 cut(s) 1154
AjuI GAANNNNNNNTTGG 4 cut(s) 989, 1021, 1190, 1222
Alw21I GWGCWC 2 cut(s) 15, 603
Alw26I GTCTC 1 cut(s) 456
AlwI GGATC 1 cut(s) 1220
AlwNI CAGNNNCTG 1 cut(s) 377
Ama87I CYCGRG 2 cut(s) 34, 650
AoxI GGCC 6 cut(s) 483, 502, 654, 716, 891, 999
ApeKI GCWGC 2 cut(s) 348, 1381
ApoI RAATTY 1 cut(s) 245
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
Asp700I GAANNNNTTC 2 cut(s) 1001, 1298
AspLEI GCGC 2 cut(s) 625, 806
AspS9I GGNCC 1 cut(s) 133
AsuHPI GGTGA 5 cut(s) 14, 950, 1020, 1178, 1363
AvaI CYCGRG 2 cut(s) 34, 650
AvaII GGWCC 1 cut(s) 133
BaeGI GKGCMC 1 cut(s) 1452
BaeI ACNNNNGTAYC 2 cut(s) 967, 1000
BalI TGGCCA 2 cut(s) 485, 893
BanI GGYRCC 1 cut(s) 1447
BanII GRGCYC 2 cut(s) 15, 603
BauI CACGAG 1 cut(s) 137
BbsI GAAGAC 1 cut(s) 570
Bbv12I GWGCWC 2 cut(s) 15, 603
BbvI GCAGC 2 cut(s) 360, 1393
BccI CCATC 6 cut(s) 221, 721, 791, 883, 1090, 1128
BcgI CGANNNNNNTGC 2 cut(s) 908, 942
BclI TGATCA 3 cut(s) 19, 121, 877
BcoDI GTCTC 1 cut(s) 456
BcuI ACTAGT 1 cut(s) 1154
BfaI CTAG 3 cut(s) 558, 1046, 1155
BfmI CTRYAG 1 cut(s) 55
BfoI RGCGCY 1 cut(s) 807
BfuAI ACCTGC 1 cut(s) 1386
BisI GCNGC 3 cut(s) 349, 807, 1382
BlsI GCNGC 3 cut(s) 350, 808, 1383
Bme18I GGWCC 1 cut(s) 133
BmeRI GACNNNNNGTC 1 cut(s) 574
BmeT110I CYCGRG 2 cut(s) 34, 650
BmgT120I GGNCC 1 cut(s) 133
BmiI GGNNCC 1 cut(s) 1449
BmrI ACTGGG 1 cut(s) 409
BmsI GCATC 2 cut(s) 579, 922
BmuI ACTGGG 1 cut(s) 409
BpiI GAAGAC 1 cut(s) 570
BplI GAGNNNNNCTC 2 cut(s) 1091, 1123
BpuEI CTTGAG 3 cut(s) 483, 755, 1320
BsaWI WCCGGW 1 cut(s) 446
Bsc4I CCNNNNNNNGG 1 cut(s) 316
Bse1I ACTGG 3 cut(s) 404, 712, 829
Bse3DI GCAATG 1 cut(s) 747
BseGI GGATG 1 cut(s) 673
BseLI CCNNNNNNNGG 1 cut(s) 316
BseMI GCAATG 1 cut(s) 747
BseMII CTCAG 1 cut(s) 861
BseNI ACTGG 3 cut(s) 404, 712, 829
BseSI GKGCMC 1 cut(s) 1452
BseXI GCAGC 2 cut(s) 360, 1393
BsgI GTGCAG 1 cut(s) 1400
BshFI GGCC 6 cut(s) 485, 504, 656, 718, 893, 1001
BshNI GGYRCC 1 cut(s) 1447
BsiHKAI GWGCWC 2 cut(s) 15, 603
BsiHKCI CYCGRG 2 cut(s) 34, 650
BsiSI CCGG 1 cut(s) 447
BslI CCNNNNNNNGG 1 cut(s) 316
BsmAI GTCTC 1 cut(s) 456
BsnI GGCC 6 cut(s) 485, 504, 656, 718, 893, 1001
BsoBI CYCGRG 2 cut(s) 34, 650
Bsp1286I GDGCHC 3 cut(s) 15, 603, 1452
Bsp143I GATC 4 cut(s) 19, 121, 877, 1212
BspACI CCGC 2 cut(s) 780, 807
BspANI GGCC 6 cut(s) 485, 504, 656, 718, 893, 1001
BspCNI CTCAG 1 cut(s) 860
BspLI GGNNCC 1 cut(s) 1449
BspMI ACCTGC 1 cut(s) 1386
BspPI GGATC 1 cut(s) 1220
BspT107I GGYRCC 1 cut(s) 1447
BsrBI CCGCTC 1 cut(s) 809
BsrDI GCAATG 1 cut(s) 747
BsrI ACTGG 3 cut(s) 404, 712, 829
BssMI GATC 4 cut(s) 19, 121, 877, 1212
BssSI CACGAG 1 cut(s) 137
Bst2BI CACGAG 1 cut(s) 137
Bst4CI ACNGT 2 cut(s) 56, 790
Bst6I CTCTTC 3 cut(s) 336, 444, 1050
BstC8I GCNNGC 1 cut(s) 549
BstDEI CTNAG 1 cut(s) 847
BstEII GGTNACC 1 cut(s) 775
BstENI CCTNNNNNAGG 1 cut(s) 314
BstF5I GGATG 1 cut(s) 673
BstH2I RGCGCY 1 cut(s) 807
BstHHI GCGC 2 cut(s) 625, 806
BstKTI GATC 4 cut(s) 22, 124, 880, 1215
BstMAI GTCTC 1 cut(s) 456
BstMBI GATC 4 cut(s) 19, 121, 877, 1212
BstMWI GCNNNNNNNGC 6 cut(s) 389, 598, 641, 812, 941, 970
BstNSI RCATGY 1 cut(s) 973
BstPI GGTNACC 1 cut(s) 775
BstSFI CTRYAG 1 cut(s) 55
BstSLI GKGCMC 1 cut(s) 1452
BstV1I GCAGC 2 cut(s) 360, 1393
BstV2I GAAGAC 1 cut(s) 570
BsuRI GGCC 6 cut(s) 485, 504, 656, 718, 893, 1001
BtsCI GGATG 1 cut(s) 673
BtsI GCAGTG 1 cut(s) 208
BtsIMutI CAGTG 2 cut(s) 208, 719
BveI ACCTGC 1 cut(s) 1386
Cac8I GCNNGC 1 cut(s) 549
CaiI CAGNNNCTG 1 cut(s) 377
CfoI GCGC 2 cut(s) 625, 806
Cfr13I GGNCC 1 cut(s) 133
Csp6I GTAC 2 cut(s) 108, 1049
CspCI CAANNNNNGTGG 2 cut(s) 100, 135
CviAII CATG 9 cut(s) 178, 287, 542, 680, 700, 952, 970, 1015, 1245
CviQI GTAC 2 cut(s) 108, 1049
DdeI CTNAG 1 cut(s) 847
DpnI GATC 4 cut(s) 21, 123, 879, 1214
DpnII GATC 4 cut(s) 19, 121, 877, 1212
DrdI GACNNNNNNGTC 1 cut(s) 680
DriI GACNNNNNGTC 1 cut(s) 574
DseDI GACNNNNNNGTC 1 cut(s) 680
EaeI YGGCCR 2 cut(s) 483, 891
Eam1104I CTCTTC 3 cut(s) 336, 444, 1050
Eam1105I GACNNNNNGTC 1 cut(s) 574
EarI CTCTTC 3 cut(s) 336, 444, 1050
Ecl136II GAGCTC 2 cut(s) 13, 601
Eco147I AGGCCT 2 cut(s) 504, 1001
Eco24I GRGCYC 2 cut(s) 15, 603
Eco47I GGWCC 1 cut(s) 133
Eco53kI GAGCTC 2 cut(s) 13, 601
Eco57I CTGAAG 1 cut(s) 399
Eco88I CYCGRG 2 cut(s) 34, 650
Eco91I GGTNACC 1 cut(s) 775
EcoICRI GAGCTC 2 cut(s) 13, 601
EcoNI CCTNNNNNAGG 1 cut(s) 314
EcoO65I GGTNACC 1 cut(s) 775
EcoRI GAATTC 1 cut(s) 245
EcoT38I GRGCYC 2 cut(s) 15, 603
FaeI CATG 9 cut(s) 181, 290, 545, 683, 703, 955, 973, 1018, 1248
FalI AAGNNNNNCTT 2 cut(s) 989, 1021
FatI CATG 9 cut(s) 177, 286, 541, 679, 699, 951, 969, 1014, 1244
FbaI TGATCA 3 cut(s) 19, 121, 877
Fnu4HI GCNGC 3 cut(s) 349, 807, 1382
FokI GGATG 1 cut(s) 680
FriOI GRGCYC 2 cut(s) 15, 603
Fsp4HI GCNGC 3 cut(s) 349, 807, 1382
FspBI CTAG 3 cut(s) 558, 1046, 1155
FspI TGCGCA 1 cut(s) 624
GlaI GCGC 2 cut(s) 624, 805
GluI GCNGC 3 cut(s) 349, 807, 1382
HaeII RGCGCY 1 cut(s) 807
HaeIII GGCC 6 cut(s) 485, 504, 656, 718, 893, 1001
HapII CCGG 1 cut(s) 447
HhaI GCGC 2 cut(s) 625, 806
Hin1II CATG 9 cut(s) 181, 290, 545, 683, 703, 955, 973, 1018, 1248
Hin6I GCGC 2 cut(s) 623, 804
HinP1I GCGC 2 cut(s) 623, 804
HincII GTYRAC 3 cut(s) 733, 793, 1483
HindII GTYRAC 3 cut(s) 733, 793, 1483
HpaII CCGG 1 cut(s) 447
HphI GGTGA 5 cut(s) 14, 950, 1020, 1178, 1363
Hpy166II GTNNAC 3 cut(s) 733, 793, 1483
Hpy188I TCNGA 8 cut(s) 70, 91, 133, 327, 588, 850, 882, 1111
Hpy188III TCNNGA 5 cut(s) 224, 558, 650, 1216, 1419
Hpy8I GTNNAC 3 cut(s) 733, 793, 1483
Hpy99I CGWCG 1 cut(s) 759
HpyCH4III ACNGT 2 cut(s) 56, 790
HpyCH4IV ACGT 1 cut(s) 488
HpyCH4V TGCA 5 cut(s) 740, 935, 951, 1097, 1381
HpyF10VI GCNNNNNNNGC 6 cut(s) 389, 598, 641, 812, 941, 970
HpyF3I CTNAG 1 cut(s) 847
HpySE526I ACGT 1 cut(s) 488
Hsp92II CATG 9 cut(s) 181, 290, 545, 683, 703, 955, 973, 1018, 1248
HspAI GCGC 2 cut(s) 623, 804
Ksp22I TGATCA 3 cut(s) 19, 121, 877
Kzo9I GATC 4 cut(s) 19, 121, 877, 1212
LmnI GCTCC 3 cut(s) 162, 374, 597
Lsp1109I GCAGC 2 cut(s) 360, 1393
LweI GCATC 2 cut(s) 579, 922
MaeI CTAG 3 cut(s) 558, 1046, 1155
MaeII ACGT 1 cut(s) 488
MaeIII GTNAC 2 cut(s) 775, 1150
MalI GATC 4 cut(s) 21, 123, 879, 1214
MbiI CCGCTC 1 cut(s) 809
MboI GATC 4 cut(s) 19, 121, 877, 1212
MfeI CAATTG 1 cut(s) 946
MhlI GDGCHC 3 cut(s) 15, 603, 1452
MlsI TGGCCA 2 cut(s) 485, 893
MluCI AATT 7 cut(s) 245, 304, 479, 859, 946, 1022, 1129
MluNI TGGCCA 2 cut(s) 485, 893
MlyI GAGTC 3 cut(s) 860, 1136, 1363
MmeI TCCRAC 1 cut(s) 1391
Mox20I TGGCCA 2 cut(s) 485, 893
MroXI GAANNNNTTC 2 cut(s) 1001, 1298
MscI TGGCCA 2 cut(s) 485, 893
MseI TTAA 7 cut(s) 353, 522, 615, 837, 1184, 1316, 1454
MslI CAYNNNNRTG 1 cut(s) 1445
Msp20I TGGCCA 2 cut(s) 485, 893
MspA1I CMGCKG 1 cut(s) 551
MspI CCGG 1 cut(s) 447
MunI CAATTG 1 cut(s) 946
MwoI GCNNNNNNNGC 6 cut(s) 389, 598, 641, 812, 941, 970
NdeII GATC 4 cut(s) 19, 121, 877, 1212
NlaIII CATG 9 cut(s) 181, 290, 545, 683, 703, 955, 973, 1018, 1248
NlaIV GGNNCC 1 cut(s) 1449
NsbI TGCGCA 1 cut(s) 624
NspI RCATGY 1 cut(s) 973
PaeR7I CTCGAG 1 cut(s) 650
PceI AGGCCT 2 cut(s) 504, 1001
PdmI GAANNNNTTC 2 cut(s) 1001, 1298
PfeI GAWTC 7 cut(s) 65, 71, 167, 188, 322, 646, 955
PkrI GCNGC 3 cut(s) 350, 808, 1383
PleI GAGTC 3 cut(s) 859, 1136, 1362
PpsI GAGTC 3 cut(s) 859, 1136, 1362
Psp124BI GAGCTC 2 cut(s) 15, 603
PspEI GGTNACC 1 cut(s) 775
PspN4I GGNNCC 1 cut(s) 1449
PspPI GGNCC 1 cut(s) 133
PstNI CAGNNNCTG 1 cut(s) 377
PvuII CAGCTG 1 cut(s) 551
RsaI GTAC 2 cut(s) 109, 1050
RsaNI GTAC 2 cut(s) 108, 1049
RseI CAYNNNNRTG 1 cut(s) 1445
SacI GAGCTC 2 cut(s) 15, 603
SaqAI TTAA 7 cut(s) 353, 522, 615, 837, 1184, 1316, 1454
SatI GCNGC 3 cut(s) 349, 807, 1382
Sau3AI GATC 4 cut(s) 19, 121, 877, 1212
Sau96I GGNCC 1 cut(s) 133
SchI GAGTC 3 cut(s) 860, 1136, 1363
SduI GDGCHC 3 cut(s) 15, 603, 1452
SfaNI GCATC 2 cut(s) 579, 922
SfcI CTRYAG 1 cut(s) 55
Sfr274I CTCGAG 1 cut(s) 650
SinI GGWCC 1 cut(s) 133
SlaI CTCGAG 1 cut(s) 650
SmiMI CAYNNNNRTG 1 cut(s) 1445
SmlI CTYRAG 4 cut(s) 498, 650, 770, 1335
SmoI CTYRAG 4 cut(s) 498, 650, 770, 1335
SpeI ACTAGT 1 cut(s) 1154
Sse9I AATT 7 cut(s) 245, 304, 479, 859, 946, 1022, 1129
SseBI AGGCCT 2 cut(s) 504, 1001
SsiI CCGC 2 cut(s) 780, 807
SspI AATATT 1 cut(s) 993
SspMI CTAG 3 cut(s) 558, 1046, 1155
SstI GAGCTC 2 cut(s) 15, 603
StuI AGGCCT 2 cut(s) 504, 1001
TaaI ACNGT 2 cut(s) 56, 790
TaiI ACGT 1 cut(s) 491
TaqI TCGA 3 cut(s) 293, 651, 918
TaqII GACCGA 1 cut(s) 209
TasI AATT 7 cut(s) 245, 304, 479, 859, 946, 1022, 1129
TatI WGTACW 1 cut(s) 1048
TauI GCSGC 1 cut(s) 809
TfiI GAWTC 7 cut(s) 65, 71, 167, 188, 322, 646, 955
Tru1I TTAA 7 cut(s) 353, 522, 615, 837, 1184, 1316, 1454
Tru9I TTAA 7 cut(s) 353, 522, 615, 837, 1184, 1316, 1454
TscAI CASTG 2 cut(s) 208, 719
TseI GCWGC 2 cut(s) 348, 1381
TspDTI ATGAA 4 cut(s) 497, 570, 668, 711
TspRI CASTG 2 cut(s) 208, 719
VpaK11BI GGWCC 1 cut(s) 133
XagI CCTNNNNNAGG 1 cut(s) 314
XapI RAATTY 1 cut(s) 245
XbaI TCTAGA 1 cut(s) 557
XceI RCATGY 1 cut(s) 973
XhoI CTCGAG 1 cut(s) 650
XmnI GAANNNNTTC 2 cut(s) 1001, 1298
XspI CTAG 3 cut(s) 558, 1046, 1155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.