Rroxscaffold_3G00231710

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0003965
Physical Location & Seq
Reverse (-)
17096250 .. 17099937
3688 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00231710.1

Sequence Viewer

Length: 369 bp
ATGGAGAGAGAGATAGCTGATATTCACAAAAGGAGATTGTCACCTCATCATCTGAGTACAAGAGTTCGTATAAGTTCAGATTCAATGAGTAAAATAGAGGCTGAACTTGAAGCTGAATTTCAAAGGTTGGGGCTAAACAGGAATACTTCTACTTTGGAGAGAAGATTAACTGATCTTGATGAGTCCAAGCTGCTATCATCACGATTGAAGGGGTTATCCCCTGGTGGAGATCTTCCCTTCCTATTTATTAGAGTTTTGGTGAAAATTCCGAGCTTCGAAGCCATTCACAAGCAGGAAAAAATCATTGAAGCTTCCTATGGAGTCATGATTGCTCAGGTTGATCTTGGAGTTGAAATCCCACTCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

122

Amino Acids

13.86

Weight (kDa)

8.09

Isoelectric Point (pI)

57.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 83 - 121 3.1e-06 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 116, 264
AfaI GTAC 1 cut(s) 58
AgsI TTSAA 6 cut(s) 84, 110, 122, 208, 308, 353
AjnI CCWGG 1 cut(s) 220
AluBI AGCT 5 cut(s) 17, 113, 190, 273, 311
AluI AGCT 5 cut(s) 17, 113, 190, 273, 311
ApeKI GCWGC 1 cut(s) 190
ApoI RAATTY 2 cut(s) 116, 264
Asp700I GAANNNNTTC 1 cut(s) 282
AsuHPI GGTGA 2 cut(s) 33, 271
AsuII TTCGAA 1 cut(s) 276
BbvI GCAGC 1 cut(s) 177
BciT130I CCWGG 1 cut(s) 222
BglII AGATCT 1 cut(s) 229
BisI GCNGC 1 cut(s) 191
BlsI GCNGC 1 cut(s) 192
Bme1390I CCNGG 1 cut(s) 222
BmrFI CCNGG 1 cut(s) 222
Bpu10I CCTNAGC 1 cut(s) 333
Bpu14I TTCGAA 1 cut(s) 276
BsaJI CCNNGG 1 cut(s) 220
BseBI CCWGG 1 cut(s) 222
BseDI CCNNGG 1 cut(s) 220
BseMII CTCAG 2 cut(s) 44, 347
BseXI GCAGC 1 cut(s) 177
Bsp119I TTCGAA 1 cut(s) 276
Bsp143I GATC 3 cut(s) 172, 229, 340
BspCNI CTCAG 2 cut(s) 45, 346
BspHI TCATGA 1 cut(s) 324
BspT104I TTCGAA 1 cut(s) 276
BssECI CCNNGG 1 cut(s) 220
BssMI GATC 3 cut(s) 172, 229, 340
Bst2UI CCWGG 1 cut(s) 222
BstBI TTCGAA 1 cut(s) 276
BstDEI CTNAG 2 cut(s) 53, 333
BstKTI GATC 3 cut(s) 175, 232, 343
BstMBI GATC 3 cut(s) 172, 229, 340
BstNI CCWGG 1 cut(s) 222
BstSCI CCNGG 1 cut(s) 220
BstV1I GCAGC 1 cut(s) 177
BstX2I RGATCY 1 cut(s) 229
BstYI RGATCY 1 cut(s) 229
CciI TCATGA 1 cut(s) 324
Csp6I GTAC 1 cut(s) 57
CviAII CATG 1 cut(s) 325
CviJI RGCY 8 cut(s) 17, 101, 113, 133, 190, 273, 281, 311
CviKI_1 RGCY 8 cut(s) 17, 101, 113, 133, 190, 273, 281, 311
CviQI GTAC 1 cut(s) 57
DdeI CTNAG 2 cut(s) 53, 333
DpnI GATC 3 cut(s) 174, 231, 342
DpnII GATC 3 cut(s) 172, 229, 340
EcoRII CCWGG 1 cut(s) 220
FaeI CATG 1 cut(s) 328
FaiI YATR 3 cut(s) 71, 318, 326
FatI CATG 1 cut(s) 324
Fnu4HI GCNGC 1 cut(s) 191
Fsp4HI GCNGC 1 cut(s) 191
GluI GCNGC 1 cut(s) 191
Hin1II CATG 1 cut(s) 328
HindIII AAGCTT 1 cut(s) 309
HinfI GANTC 3 cut(s) 80, 182, 321
HphI GGTGA 2 cut(s) 33, 271
Hpy188I TCNGA 3 cut(s) 54, 79, 270
Hpy188III TCNNGA 3 cut(s) 176, 201, 325
HpyAV CCTTC 2 cut(s) 202, 247
HpyF3I CTNAG 2 cut(s) 53, 333
Hsp92II CATG 1 cut(s) 328
Kzo9I GATC 3 cut(s) 172, 229, 340
LpnPI CCDG 5 cut(s) 124, 207, 234, 278, 320
Lsp1109I GCAGC 1 cut(s) 177
MaeIII GTNAC 1 cut(s) 39
MalI GATC 3 cut(s) 174, 231, 342
MboI GATC 3 cut(s) 172, 229, 340
MboII GAAGA 2 cut(s) 174, 224
MflI RGATCY 1 cut(s) 229
MluCI AATT 2 cut(s) 116, 264
MlyI GAGTC 2 cut(s) 191, 330
MnlI CCTC 2 cut(s) 54, 91
MroXI GAANNNNTTC 1 cut(s) 282
MseI TTAA 1 cut(s) 167
MspR9I CCNGG 1 cut(s) 222
MvaI CCWGG 1 cut(s) 222
NdeII GATC 3 cut(s) 172, 229, 340
NlaIII CATG 1 cut(s) 328
NmuCI GTSAC 1 cut(s) 39
NspV TTCGAA 1 cut(s) 276
PagI TCATGA 1 cut(s) 324
PdmI GAANNNNTTC 1 cut(s) 282
PfeI GAWTC 1 cut(s) 80
PkrI GCNGC 1 cut(s) 192
PleI GAGTC 2 cut(s) 190, 329
PpsI GAGTC 2 cut(s) 190, 329
Psp6I CCWGG 1 cut(s) 220
PspGI CCWGG 1 cut(s) 220
PsuI RGATCY 1 cut(s) 229
RsaI GTAC 1 cut(s) 58
RsaNI GTAC 1 cut(s) 57
SaqAI TTAA 1 cut(s) 167
SatI GCNGC 1 cut(s) 191
Sau3AI GATC 3 cut(s) 172, 229, 340
SchI GAGTC 2 cut(s) 191, 330
ScrFI CCNGG 1 cut(s) 222
SetI ASST 8 cut(s) 19, 46, 115, 128, 192, 275, 313, 339
SfuI TTCGAA 1 cut(s) 276
Sse9I AATT 2 cut(s) 116, 264
StyD4I CCNGG 1 cut(s) 220
TaqI TCGA 1 cut(s) 276
TasI AATT 2 cut(s) 116, 264
TatI WGTACW 1 cut(s) 56
TfiI GAWTC 1 cut(s) 80
Tru1I TTAA 1 cut(s) 167
Tru9I TTAA 1 cut(s) 167
TseFI GTSAC 1 cut(s) 39
TseI GCWGC 1 cut(s) 190
Tsp45I GTSAC 1 cut(s) 39
XapI RAATTY 2 cut(s) 116, 264
XmnI GAANNNNTTC 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.