RchiOBHm_Chr2g0087151

heparanase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001523
Physical Location & Seq
Forward (+)
2020593 .. 2023676
3084 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46260

Sequence Viewer

Length: 1668 bp
ATGGATTCTCAAATCTGGAGAACAGGGATGTGTTTCTGGGTGTGTTTGTTTGGTTTCTTGAGCTTCATTCATTGTGTGAGTTCAGAAGGAGAGGGAATAGGAGGAGGTAGTACTGAAGGTTTGGTTAGAATTGATGGGAAAGAACCCATTGGGAAAATTGATGAAGATTTTGTTTGTGCAACTTTGGATTGGTGGCCTCCTGAGAAATGTGACTATGGAACTTGCAGCTGGGGCAAGGCTTCTCTCCTTAATCTGGATCTAAGTAACACTATCTTATCAAATGCCATAAAGGCTTTTTCACCATTGAAACTTAGATTGGGAGGTACCTTGCAAGATAAAGTCATATATGATACAGAAGACAATAAAGAACCTTGCCCAGTTCCTTTTGTTAAAAATACCACAGCTATGTTTGGATTTAATAAGGGGTGCTTACCCATGAAGAGATGGGATGAATTAAACTCCTTTTTTCTGAGAACAGGGGCTAAGATTATCTTTGGATTAAATGCCCTCCCCGGACGAACTTTACAGAGTGATGGCTCTGCAACTGGAGCTTGGGACTTTACCAATGCCGAATCTTTCATTCGGTACACTGTCAAACACAACTACTCTATAGCTGGATGGGAGCTAGGGAATGAGCTATGTGGGCGTGGAGTTGGAACAGCAGTAGCAGCAAGTCAGTATGTCAATGATACAGCTTCTCTGCATAACATAGTACAGAATGTATACAAGGGTGTTGGACCAAAGCCACTAATCTTGTCACCTGGAGGGTTCTTTGATGCACCTTGGTTCAAAGAACTTGCAGATAAAACCACCACTTCATTAGATGCGGTCACTCACCATATATATAATCTAGGGCCAGGGGTTGATCAACACCTTATTGAAAAGATTCTTGATCCGGCGTATCTGGATGGTATTGCTAACACATTTAGCAGCCTTCGAAACATCCTAAAGACCTCTGCAACTTCAGCAAGTGCATGGGTTGGGGAAGCAGGAGGTGCTTATAACAGCGGTCATCATCTTGTCACGGATGCATTCGTGTTCAGTTTCTGGTATTTGGATCAGCTTGGTATGTCTGCATCTTATGATACCAAAACTTATTGCAGACAGACATTGATTGGTGGAAACTATGGTTTACTCAACACCACTACCTTTGTTCCCAATCCAGACTACTACAGTGCTCTTCTTTGGCACCGATTAATGGGAAGAAATGTACTGTCAACGAGGTTTGCTGGACCCAAAAAGATACGTGCTTATACACACTGTGCAAAACAATCTAAAGGGATTGTAGTACTATTGATCAACCTACACAACACCACCATTGTTCAGGCCAAGGTTGCCTTCAACAGTACCTGGAGTTTGCGACATAGACACAAATATCACAAGTCTCATAGATCACATATGCTGAAGAAGCACCATAGCGGTCTAAGAAGCTCAACAGAAAGAGTGAGAGGTGATACAGAAAGAGAAGAATACCATTTGACACCAAAGGATGGAAATATACAAAGCCAAACCATGCTGCTTAATGGAAACGCTTTGAATGTGGATTCATCTGGGAACATACCTACATTAGAACCTATTTTTGTAAATTCATCGCAACCGATAATTGTTGCTCCATTTTCAATTGTATTTGTTCACATACCATACGTTGTTCCCCCTGCTTGCAGGTAG

Protein Analysis

555

Amino Acids

61.36

Weight (kDa)

8.46

Isoelectric Point (pI)

39.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_79n PF03662 40 - 356 3.1e-156 Glycosyl hydrolase family 79, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1002
Acc36I ACCTGC 1 cut(s) 1653
Acc65I GGTACC 1 cut(s) 323
AccB1I GGYRCC 2 cut(s) 323, 1188
AccB7I CCANNNNNTGG 1 cut(s) 1490
AccI GTMKAC 1 cut(s) 723
AciI CCGC 3 cut(s) 827, 1008, 1419
AclWI GGATC 3 cut(s) 264, 887, 1065
AcsI RAATTY 1 cut(s) 1585
AcuI CTGAAG 3 cut(s) 135, 948, 1424
AdeI CACNNNGTG 1 cut(s) 1262
AfaI GTAC 7 cut(s) 112, 325, 587, 714, 1212, 1290, 1348
AfiI CCNNNNNNNGG 3 cut(s) 253, 1198, 1490
AgsI TTSAA 6 cut(s) 307, 790, 881, 1342, 1537, 1620
AjnI CCWGG 3 cut(s) 760, 856, 1349
AjuI GAANNNNNNNTTGG 4 cut(s) 299, 331, 1322, 1354
Alw21I GWGCWC 1 cut(s) 1180
Alw26I GTCTC 1 cut(s) 1389
AlwI GGATC 3 cut(s) 264, 887, 1065
AlwNI CAGNNNCTG 2 cut(s) 1047, 1350
AoxI GGCC 3 cut(s) 194, 854, 1326
ApeKI GCWGC 4 cut(s) 225, 668, 930, 1516
ApoI RAATTY 1 cut(s) 1585
AseI ATTAAT 1 cut(s) 1196
Asp700I GAANNNNTTC 1 cut(s) 885
Asp718I GGTACC 1 cut(s) 323
AspS9I GGNCC 3 cut(s) 737, 854, 1232
AsuC2I CCSGG 1 cut(s) 513
AsuHPI GGTGA 4 cut(s) 291, 750, 827, 1463
AsuII TTCGAA 1 cut(s) 937
AvaII GGWCC 2 cut(s) 737, 1232
BanI GGYRCC 2 cut(s) 323, 1188
BbsI GAAGAC 1 cut(s) 363
Bbv12I GWGCWC 1 cut(s) 1180
BbvI GCAGC 4 cut(s) 237, 680, 942, 1503
BccI CCATC 6 cut(s) 128, 438, 527, 612, 902, 1484
BciT130I CCWGG 3 cut(s) 762, 858, 1351
BclI TGATCA 2 cut(s) 865, 1296
BcnI CCSGG 1 cut(s) 513
BcoDI GTCTC 1 cut(s) 1389
BfaI CTAG 2 cut(s) 626, 851
BfmI CTRYAG 2 cut(s) 609, 1171
BfuAI ACCTGC 1 cut(s) 1653
BglI GCCNNNNNGGC 1 cut(s) 290
BisI GCNGC 4 cut(s) 226, 669, 931, 1517
BlsI GCNGC 4 cut(s) 227, 670, 932, 1518
BmcAI AGTACT 2 cut(s) 112, 1290
Bme1390I CCNGG 4 cut(s) 513, 762, 858, 1351
Bme18I GGWCC 2 cut(s) 737, 1232
BmgT120I GGNCC 3 cut(s) 737, 854, 1232
BmiI GGNNCC 3 cut(s) 325, 1190, 1234
BmrFI CCNGG 4 cut(s) 513, 762, 858, 1351
BmrI ACTGGG 1 cut(s) 371
BmsI GCATC 4 cut(s) 766, 814, 1018, 1085
BmuI ACTGGG 1 cut(s) 371
BpiI GAAGAC 1 cut(s) 363
BpmI CTGGAG 4 cut(s) 37, 567, 783, 1372
Bpu14I TTCGAA 1 cut(s) 937
BpuEI CTTGAG 1 cut(s) 79
BpuMI CCSGG 1 cut(s) 513
BsaAI YACGTR 1 cut(s) 1247
BsaJI CCNNGG 4 cut(s) 511, 782, 857, 1329
Bsc4I CCNNNNNNNGG 3 cut(s) 253, 1198, 1490
Bse1I ACTGG 2 cut(s) 377, 550
BseBI CCWGG 3 cut(s) 762, 858, 1351
BseDI CCNNGG 4 cut(s) 511, 782, 857, 1329
BseGI GGATG 7 cut(s) 33, 454, 623, 913, 942, 1033, 1495
BseLI CCNNNNNNNGG 3 cut(s) 253, 1198, 1490
BseMII CTCAG 2 cut(s) 192, 461
BseNI ACTGG 2 cut(s) 377, 550
BseRI GAGGAG 1 cut(s) 117
BseXI GCAGC 4 cut(s) 237, 680, 942, 1503
BseYI CCCAGC 1 cut(s) 228
BshFI GGCC 3 cut(s) 196, 856, 1328
BshNI GGYRCC 2 cut(s) 323, 1188
BsiHKAI GWGCWC 1 cut(s) 1180
BsiSI CCGG 2 cut(s) 513, 896
BslFI GGGAC 1 cut(s) 569
BslI CCNNNNNNNGG 3 cut(s) 253, 1198, 1490
BsmAI GTCTC 1 cut(s) 1389
BsmFI GGGAC 1 cut(s) 569
BsmI GAATGC 1 cut(s) 1031
BsnI GGCC 3 cut(s) 196, 856, 1328
Bsp119I TTCGAA 1 cut(s) 937
Bsp1286I GDGCHC 1 cut(s) 1180
Bsp143I GATC 6 cut(s) 256, 865, 892, 1057, 1296, 1391
BspACI CCGC 3 cut(s) 827, 1008, 1419
BspANI GGCC 3 cut(s) 196, 856, 1328
BspCNI CTCAG 2 cut(s) 193, 462
BspLI GGNNCC 3 cut(s) 325, 1190, 1234
BspMI ACCTGC 1 cut(s) 1653
BspPI GGATC 3 cut(s) 264, 887, 1065
BspQI GCTCTTC 1 cut(s) 1185
BspT104I TTCGAA 1 cut(s) 937
BspT107I GGYRCC 2 cut(s) 323, 1188
BsrI ACTGG 2 cut(s) 377, 550
BssECI CCNNGG 4 cut(s) 511, 782, 857, 1329
BssMI GATC 6 cut(s) 256, 865, 892, 1057, 1296, 1391
BssNAI GTATAC 1 cut(s) 724
BssT1I CCWWGG 2 cut(s) 782, 1329
Bst1107I GTATAC 1 cut(s) 724
Bst2UI CCWGG 3 cut(s) 762, 858, 1351
Bst4CI ACNGT 5 cut(s) 592, 1175, 1215, 1262, 1346
Bst6I CTCTTC 2 cut(s) 434, 1185
BstAPI GCANNNNNTGC 1 cut(s) 995
BstBAI YACGTR 1 cut(s) 1247
BstBI TTCGAA 1 cut(s) 937
BstC8I GCNNGC 1 cut(s) 1660
BstDEI CTNAG 6 cut(s) 201, 260, 311, 470, 483, 1424
BstF5I GGATG 7 cut(s) 33, 454, 623, 913, 942, 1033, 1495
BstKTI GATC 6 cut(s) 259, 868, 895, 1060, 1299, 1394
BstMAI GTCTC 1 cut(s) 1389
BstMBI GATC 6 cut(s) 256, 865, 892, 1057, 1296, 1391
BstMWI GCNNNNNNNGC 9 cut(s) 231, 290, 548, 643, 668, 965, 995, 1334, 1408
BstNI CCWGG 3 cut(s) 762, 858, 1351
BstSCI CCNGG 4 cut(s) 511, 760, 856, 1349
BstSFI CTRYAG 2 cut(s) 609, 1171
BstV1I GCAGC 4 cut(s) 237, 680, 942, 1503
BstV2I GAAGAC 1 cut(s) 363
BstX2I RGATCY 1 cut(s) 256
BstYI RGATCY 1 cut(s) 256
BstZ17I GTATAC 1 cut(s) 724
BsuRI GGCC 3 cut(s) 196, 856, 1328
BtgZI GCGATG 1 cut(s) 1575
BtsCI GGATG 7 cut(s) 33, 454, 623, 913, 942, 1033, 1495
BtsIMutI CAGTG 3 cut(s) 588, 1180, 1258
BveI ACCTGC 1 cut(s) 1653
Cac8I GCNNGC 1 cut(s) 1660
CaiI CAGNNNCTG 2 cut(s) 1047, 1350
Cfr13I GGNCC 3 cut(s) 737, 854, 1232
Csp6I GTAC 7 cut(s) 111, 324, 586, 713, 1211, 1289, 1347
CspCI CAANNNNNGTGG 4 cut(s) 735, 770, 1132, 1167
CviAII CATG 3 cut(s) 436, 975, 1513
CviQI GTAC 7 cut(s) 111, 324, 586, 713, 1211, 1289, 1347
DdeI CTNAG 6 cut(s) 201, 260, 311, 470, 483, 1424
DpnI GATC 6 cut(s) 258, 867, 894, 1059, 1298, 1393
DpnII GATC 6 cut(s) 256, 865, 892, 1057, 1296, 1391
DraIII CACNNNGTG 1 cut(s) 1262
Eam1104I CTCTTC 2 cut(s) 434, 1185
EarI CTCTTC 2 cut(s) 434, 1185
Eco130I CCWWGG 2 cut(s) 782, 1329
Eco47I GGWCC 2 cut(s) 737, 1232
Eco57I CTGAAG 3 cut(s) 135, 948, 1424
EcoRII CCWGG 3 cut(s) 760, 856, 1349
EcoT14I CCWWGG 2 cut(s) 782, 1329
EcoT22I ATGCAT 1 cut(s) 1033
ErhI CCWWGG 2 cut(s) 782, 1329
FaeI CATG 3 cut(s) 439, 978, 1516
FalI AAGNNNNNCTT 6 cut(s) 413, 445, 476, 508, 1322, 1354
FaqI GGGAC 1 cut(s) 569
FatI CATG 3 cut(s) 435, 974, 1512
FauNDI CATATG 1 cut(s) 1398
FbaI TGATCA 2 cut(s) 865, 1296
FblI GTMKAC 1 cut(s) 723
Fnu4HI GCNGC 4 cut(s) 226, 669, 931, 1517
FokI GGATG 7 cut(s) 40, 461, 630, 920, 929, 1040, 1502
Fsp4HI GCNGC 4 cut(s) 226, 669, 931, 1517
FspBI CTAG 2 cut(s) 626, 851
GluI GCNGC 4 cut(s) 226, 669, 931, 1517
GsaI CCCAGC 1 cut(s) 232
GsuI CTGGAG 4 cut(s) 37, 567, 783, 1372
HaeIII GGCC 3 cut(s) 196, 856, 1328
HapII CCGG 2 cut(s) 513, 896
Hin1II CATG 3 cut(s) 439, 978, 1516
HincII GTYRAC 1 cut(s) 1218
HindII GTYRAC 1 cut(s) 1218
HinfI GANTC 4 cut(s) 5, 572, 886, 1544
HpaII CCGG 2 cut(s) 513, 896
HphI GGTGA 4 cut(s) 291, 750, 827, 1463
Hpy166II GTNNAC 5 cut(s) 588, 724, 1133, 1218, 1633
Hpy188I TCNGA 2 cut(s) 85, 471
Hpy188III TCNNGA 7 cut(s) 16, 58, 200, 254, 890, 905, 1163
Hpy8I GTNNAC 5 cut(s) 588, 724, 1133, 1218, 1633
HpyAV CCTTC 4 cut(s) 80, 110, 944, 1348
HpyCH4III ACNGT 5 cut(s) 592, 1175, 1215, 1262, 1346
HpyCH4IV ACGT 2 cut(s) 1246, 1644
HpyF10VI GCNNNNNNNGC 9 cut(s) 231, 290, 548, 643, 668, 965, 995, 1334, 1408
HpyF3I CTNAG 6 cut(s) 201, 260, 311, 470, 483, 1424
HpySE526I ACGT 2 cut(s) 1246, 1644
Hsp92II CATG 3 cut(s) 439, 978, 1516
KpnI GGTACC 1 cut(s) 327
Ksp22I TGATCA 2 cut(s) 865, 1296
Kzo9I GATC 6 cut(s) 256, 865, 892, 1057, 1296, 1391
LguI GCTCTTC 1 cut(s) 1185
LmnI GCTCC 3 cut(s) 548, 622, 1615
Lsp1109I GCAGC 4 cut(s) 237, 680, 942, 1503
LweI GCATC 4 cut(s) 766, 814, 1018, 1085
MaeI CTAG 2 cut(s) 626, 851
MaeII ACGT 2 cut(s) 1246, 1644
MaeIII GTNAC 5 cut(s) 209, 263, 756, 829, 1021
MalI GATC 6 cut(s) 258, 867, 894, 1059, 1298, 1393
MboI GATC 6 cut(s) 256, 865, 892, 1057, 1296, 1391
MboII GAAGA 7 cut(s) 176, 368, 451, 1172, 1215, 1417, 1478
MfeI CAATTG 1 cut(s) 1620
MflI RGATCY 1 cut(s) 256
MhlI GDGCHC 1 cut(s) 1180
MluCI AATT 6 cut(s) 129, 156, 452, 1585, 1602, 1620
MmeI TCCRAC 2 cut(s) 634, 715
Mph1103I ATGCAT 1 cut(s) 1033
MroXI GAANNNNTTC 1 cut(s) 885
MseI TTAA 7 cut(s) 249, 390, 417, 455, 500, 1196, 1521
MslI CAYNNNNRTG 1 cut(s) 404
MspA1I CMGCKG 2 cut(s) 228, 1008
MspI CCGG 2 cut(s) 513, 896
MspR9I CCNGG 4 cut(s) 513, 762, 858, 1351
MunI CAATTG 1 cut(s) 1620
Mva1269I GAATGC 1 cut(s) 1031
MvaI CCWGG 3 cut(s) 762, 858, 1351
MwoI GCNNNNNNNGC 9 cut(s) 231, 290, 548, 643, 668, 965, 995, 1334, 1408
NciI CCSGG 1 cut(s) 513
NdeI CATATG 1 cut(s) 1398
NdeII GATC 6 cut(s) 256, 865, 892, 1057, 1296, 1391
NlaIII CATG 3 cut(s) 439, 978, 1516
NlaIV GGNNCC 3 cut(s) 325, 1190, 1234
NmuCI GTSAC 4 cut(s) 209, 756, 829, 1021
NsiI ATGCAT 1 cut(s) 1033
NspV TTCGAA 1 cut(s) 937
PciSI GCTCTTC 1 cut(s) 1185
PctI GAATGC 1 cut(s) 1031
PdmI GAANNNNTTC 1 cut(s) 885
PfeI GAWTC 4 cut(s) 5, 572, 886, 1544
PflMI CCANNNNNTGG 1 cut(s) 1490
PkrI GCNGC 4 cut(s) 227, 670, 932, 1518
Ppu21I YACGTR 1 cut(s) 1247
PshBI ATTAAT 1 cut(s) 1196
PsiI TTATAA 1 cut(s) 1002
Psp6I CCWGG 3 cut(s) 760, 856, 1349
PspFI CCCAGC 1 cut(s) 228
PspGI CCWGG 3 cut(s) 760, 856, 1349
PspN4I GGNNCC 3 cut(s) 325, 1190, 1234
PspPI GGNCC 3 cut(s) 737, 854, 1232
PstNI CAGNNNCTG 2 cut(s) 1047, 1350
PsuI RGATCY 1 cut(s) 256
PvuII CAGCTG 1 cut(s) 228
RsaI GTAC 7 cut(s) 112, 325, 587, 714, 1212, 1290, 1348
RsaNI GTAC 7 cut(s) 111, 324, 586, 713, 1211, 1289, 1347
RseI CAYNNNNRTG 1 cut(s) 404
SapI GCTCTTC 1 cut(s) 1185
SaqAI TTAA 7 cut(s) 249, 390, 417, 455, 500, 1196, 1521
SatI GCNGC 4 cut(s) 226, 669, 931, 1517
Sau3AI GATC 6 cut(s) 256, 865, 892, 1057, 1296, 1391
Sau96I GGNCC 3 cut(s) 737, 854, 1232
ScaI AGTACT 2 cut(s) 112, 1290
ScrFI CCNGG 4 cut(s) 513, 762, 858, 1351
SduI GDGCHC 1 cut(s) 1180
SfaNI GCATC 4 cut(s) 766, 814, 1018, 1085
SfcI CTRYAG 2 cut(s) 609, 1171
SfuI TTCGAA 1 cut(s) 937
SinI GGWCC 2 cut(s) 737, 1232
SmiMI CAYNNNNRTG 1 cut(s) 404
SmlI CTYRAG 1 cut(s) 58
SmoI CTYRAG 1 cut(s) 58
Sse9I AATT 6 cut(s) 129, 156, 452, 1585, 1602, 1620
SsiI CCGC 3 cut(s) 827, 1008, 1419
SspMI CTAG 2 cut(s) 626, 851
StyD4I CCNGG 4 cut(s) 511, 760, 856, 1349
StyI CCWWGG 2 cut(s) 782, 1329
TaaI ACNGT 5 cut(s) 592, 1175, 1215, 1262, 1346
TaiI ACGT 2 cut(s) 1249, 1647
TaqI TCGA 1 cut(s) 937
TasI AATT 6 cut(s) 129, 156, 452, 1585, 1602, 1620
TatI WGTACW 4 cut(s) 110, 712, 1210, 1288
TfiI GAWTC 4 cut(s) 5, 572, 886, 1544
Tru1I TTAA 7 cut(s) 249, 390, 417, 455, 500, 1196, 1521
Tru9I TTAA 7 cut(s) 249, 390, 417, 455, 500, 1196, 1521
TscAI CASTG 3 cut(s) 595, 1180, 1265
TseFI GTSAC 4 cut(s) 209, 756, 829, 1021
TseI GCWGC 4 cut(s) 225, 668, 930, 1516
Tsp45I GTSAC 4 cut(s) 209, 756, 829, 1021
TspDTI ATGAA 9 cut(s) 55, 59, 177, 452, 465, 568, 807, 1536, 1578
TspGWI ACGGA 1 cut(s) 1040
TspRI CASTG 3 cut(s) 595, 1180, 1265
Van91I CCANNNNNTGG 1 cut(s) 1490
VpaK11BI GGWCC 2 cut(s) 737, 1232
VspI ATTAAT 1 cut(s) 1196
XapI RAATTY 1 cut(s) 1585
XmiI GTMKAC 1 cut(s) 723
XmnI GAANNNNTTC 1 cut(s) 885
XspI CTAG 2 cut(s) 626, 851
ZrmI AGTACT 2 cut(s) 112, 1290
Zsp2I ATGCAT 1 cut(s) 1033
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.