Rorug06G0272700

heparanase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001523
Physical Location & Seq
Forward (+)
42018868 .. 42019194
327 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0272700.1

Sequence Viewer

Length: 327 bp
ATGTTCGAAAAGATCCATGTGCATATCCTCAATGATTTTCAAAATGAGACTCTTCAAGCTCGATGTCAATCCAAGGACGACGATTTAGGGGTTCAACAGATTCCGGTGAGCAATGAGTTCCAGTGGCATTTTCGCATTATCATGTTCAAAACAACTCTTTTCTTCTACAATACGCAGTGGAGCGGTGGACATAAAACTTTTGATGCTTCTATTGAGAACGAATGGTTTATGAAGAGTTGCTTTAAACATTGCATATGGAAATTGGACGAAATCGGAATTTCATTGTACAGTTTCAAGTACAAGCTAGACATACCCATGTATCTCTAG

Protein Analysis

108

Amino Acids

13.08

Weight (kDa)

6.17

Isoelectric Point (pI)

35.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 6 - 98 1.6e-18 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 183
AciI CCGC 1 cut(s) 183
AclWI GGATC 1 cut(s) 7
AcsI RAATTY 1 cut(s) 276
AfaI GTAC 2 cut(s) 287, 299
AgsI TTSAA 5 cut(s) 41, 56, 95, 148, 295
AluBI AGCT 2 cut(s) 59, 304
AluI AGCT 2 cut(s) 59, 304
Alw26I GTCTC 1 cut(s) 41
AlwI GGATC 1 cut(s) 7
ApoI RAATTY 1 cut(s) 276
AsuHPI GGTGA 1 cut(s) 118
AsuII TTCGAA 1 cut(s) 6
BaeI ACNNNNGTAYC 1 cut(s) 302
BcoDI GTCTC 1 cut(s) 41
BfaI CTAG 2 cut(s) 305, 325
BmsI GCATC 1 cut(s) 193
Bpu14I TTCGAA 1 cut(s) 6
BsaBI GATNNNNATC 1 cut(s) 67
BsaJI CCNNGG 1 cut(s) 72
BsaWI WCCGGW 1 cut(s) 103
Bse1I ACTGG 1 cut(s) 121
Bse3DI GCAATG 2 cut(s) 118, 247
Bse8I GATNNNNATC 1 cut(s) 67
BseDI CCNNGG 1 cut(s) 72
BseJI GATNNNNATC 1 cut(s) 67
BseMI GCAATG 2 cut(s) 118, 247
BseNI ACTGG 1 cut(s) 121
BsiSI CCGG 1 cut(s) 104
BsmAI GTCTC 1 cut(s) 41
Bsp119I TTCGAA 1 cut(s) 6
Bsp1407I TGTACA 1 cut(s) 285
Bsp143I GATC 1 cut(s) 12
BspACI CCGC 1 cut(s) 183
BspPI GGATC 1 cut(s) 7
BspT104I TTCGAA 1 cut(s) 6
BsrBI CCGCTC 1 cut(s) 183
BsrDI GCAATG 2 cut(s) 118, 247
BsrGI TGTACA 1 cut(s) 285
BsrI ACTGG 1 cut(s) 121
BssECI CCNNGG 1 cut(s) 72
BssMI GATC 1 cut(s) 12
BssT1I CCWWGG 1 cut(s) 72
Bst4CI ACNGT 1 cut(s) 290
Bst6I CTCTTC 2 cut(s) 57, 227
BstAUI TGTACA 1 cut(s) 285
BstBI TTCGAA 1 cut(s) 6
BstKTI GATC 1 cut(s) 15
BstMAI GTCTC 1 cut(s) 41
BstMBI GATC 1 cut(s) 12
BstX2I RGATCY 1 cut(s) 12
BstYI RGATCY 1 cut(s) 12
BtsI GCAGTG 1 cut(s) 182
BtsIMutI CAGTG 2 cut(s) 128, 182
Csp6I GTAC 2 cut(s) 286, 298
CviAII CATG 3 cut(s) 17, 142, 316
CviJI RGCY 2 cut(s) 59, 304
CviKI_1 RGCY 2 cut(s) 59, 304
CviQI GTAC 2 cut(s) 286, 298
DpnI GATC 1 cut(s) 14
DpnII GATC 1 cut(s) 12
DraI TTTAAA 1 cut(s) 244
Eam1104I CTCTTC 2 cut(s) 57, 227
EarI CTCTTC 2 cut(s) 57, 227
Eco130I CCWWGG 1 cut(s) 72
EcoT14I CCWWGG 1 cut(s) 72
ErhI CCWWGG 1 cut(s) 72
FaeI CATG 3 cut(s) 20, 145, 319
FaiI YATR 9 cut(s) 18, 24, 143, 192, 230, 254, 256, 311, 317
FalI AAGNNNNNCTT 2 cut(s) 224, 256
FatI CATG 3 cut(s) 16, 141, 315
FauNDI CATATG 1 cut(s) 254
FspBI CTAG 2 cut(s) 305, 325
HapII CCGG 1 cut(s) 104
Hin1II CATG 3 cut(s) 20, 145, 319
HinfI GANTC 2 cut(s) 49, 100
HpaII CCGG 1 cut(s) 104
HphI GGTGA 1 cut(s) 118
Hpy166II GTNNAC 1 cut(s) 188
Hpy188I TCNGA 1 cut(s) 275
Hpy8I GTNNAC 1 cut(s) 188
Hpy99I CGWCG 1 cut(s) 83
HpyCH4III ACNGT 1 cut(s) 290
HpyCH4V TGCA 2 cut(s) 22, 252
Hsp92II CATG 3 cut(s) 20, 145, 319
Kzo9I GATC 1 cut(s) 12
LmnI GCTCC 1 cut(s) 180
LpnPI CCDG 2 cut(s) 117, 134
LweI GCATC 1 cut(s) 193
MaeI CTAG 2 cut(s) 305, 325
MalI GATC 1 cut(s) 14
MbiI CCGCTC 1 cut(s) 183
MboI GATC 1 cut(s) 12
MboII GAAGA 3 cut(s) 44, 154, 244
MflI RGATCY 1 cut(s) 12
MluCI AATT 2 cut(s) 260, 276
MlyI GAGTC 1 cut(s) 43
MnlI CCTC 1 cut(s) 38
MseI TTAA 1 cut(s) 243
MslI CAYNNNNRTG 2 cut(s) 140, 314
MspI CCGG 1 cut(s) 104
NdeI CATATG 1 cut(s) 254
NdeII GATC 1 cut(s) 12
NlaIII CATG 3 cut(s) 20, 145, 319
NspV TTCGAA 1 cut(s) 6
PfeI GAWTC 1 cut(s) 100
PleI GAGTC 1 cut(s) 43
PpsI GAGTC 1 cut(s) 43
PsuI RGATCY 1 cut(s) 12
RsaI GTAC 2 cut(s) 287, 299
RsaNI GTAC 2 cut(s) 286, 298
RseI CAYNNNNRTG 2 cut(s) 140, 314
SaqAI TTAA 1 cut(s) 243
Sau3AI GATC 1 cut(s) 12
SchI GAGTC 1 cut(s) 43
SetI ASST 2 cut(s) 61, 306
SfaNI GCATC 1 cut(s) 193
SfuI TTCGAA 1 cut(s) 6
SmiMI CAYNNNNRTG 2 cut(s) 140, 314
Sse9I AATT 2 cut(s) 260, 276
SsiI CCGC 1 cut(s) 183
SspMI CTAG 2 cut(s) 305, 325
StyI CCWWGG 1 cut(s) 72
TaaI ACNGT 1 cut(s) 290
TaqI TCGA 2 cut(s) 6, 61
TasI AATT 2 cut(s) 260, 276
TatI WGTACW 2 cut(s) 285, 297
TfiI GAWTC 1 cut(s) 100
Tru1I TTAA 1 cut(s) 243
Tru9I TTAA 1 cut(s) 243
TscAI CASTG 2 cut(s) 128, 182
TspDTI ATGAA 2 cut(s) 245, 270
TspRI CASTG 2 cut(s) 128, 182
XapI RAATTY 1 cut(s) 276
XspI CTAG 2 cut(s) 305, 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.