RLG00000011589

heparanase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0001523
Physical Location & Seq
Reverse (-)
10736283 .. 10739140
2858 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011589

Sequence Viewer

Length: 1605 bp
ATGCGTGCTCTGGTTCTTCTCTTCTTCGTTTGCTTCTGGCTGTACGATGTCACTCACAAACACAACTCGATCTCTGTGGCTTCACAGAGCACGGAGGAAGACACTGTCTTTGTCAATGGAACAGCTGCAATCGGAAGCATAGACGATGACTTTGTTTGTGCTACAATGGATTGGTGGCCGCCGGAGAAATGCGACTATGGAACTTGTAGCTGGGGCAATGCTTCTCTTCTTAATCTAGACCTTGGCAACGATATACTTTTGAATGCAATAAAAGCATTTTCACCCTTGAAACTTAGGCTGGGCGGCACCTTAGAAGACAAAGTCATATACCAGACAGAAGACAGTCCAAAACATCATACTTCATTTGTCAAAGATAGTTCACGGTTGTTTGGCTTCTCTGATGGTTACTTATCTATGCCTAGATGGGATGAACTAAACATTTTTTTCAAGAATGCTGGGGCTGTTGTTATATTTGGACTAAATGCACTGCAAGGCAGGACCATAGATTCCCAGGGTGCTGCTGTGGGTGATTGGAATGCGACTAATGCTGAATCTTTTATAAGATATACGGTCAACAAAGGATACACAATTCATGGTTGGGAGCTAGGAAATGAACTATGTGGGGGTGGAGTTGGAACAAGAGTCACAGCAGACCAATACGCTTCGGACATAAACTCTCTCCATAGCAAGGTGCAAGATATTTATTCAGGTTCTGAATTAGCTAAGCCACTAGTCTTGGCACCAGGAGGGTTCTTTGATTCCAGTTGGTTCTCAGATTTTGTTGATAAAGCACCTCAGTCTGTTCAAGTACTCACACACCACATATACAACCTTGGTCCGGGTAGTGCCCCAGATCTTGTTGACAAGATCCTCGATCCATCTATTCTTGATGGTATTTCCAAAACCTTCAGTGACCTACAAATGCTACTTAAGAATTCAAAAACTTCAGCAGTGGCATGGGTTGGTGAATCAGGAGGTGCTTACAACAGTGGTCACAATCTTGTTACAAATGCATTTGTATTCAGTTTCTGGTACCTGGACCAGCTTGGGATGGCAGCATCTTATGATACGAAAACATATTGCAGACAAACACTCATTGGTGGAAATTATGGCCTCCTTGACATTCAAACCTTTCAACCAAATCCTGATTATTACAGCGCTCTTCTTTGGCATCGTCTAATGGGAAACAATGTCCTGTCAACAAGCTTTTCCGGGACAAAGAAACTGCGCACTTACTCTCATTGTTCCAAGAAAACACAAGGAATCACAGTGCTGCTCATCAACTTAGACGGTAACACTACAGTTCAAGTCGATGTTTCAACTGAAAATGGTGGCAGCCGCAGTACTTTGAATTTGGAACAAGGCCTCAAGGATTCTAAAGCAACCAAGAACGATGTAAGAGAAGAATACCACTTAACAGCCAAAGATGGGGACTTGCACAGCCGAGTAATGCTTCTAAATGGGAAAGAACTGACCGTAGATTCAGCAGGGCAAATCCCAAGCTTGGAGCCAATGATAGTAGGGATGTCAGATCCCACCTCTGTGGCACCTTTCTCTATTGTATTTGTTCACATTACCAACATAACTGTCCCTGCTTGTAAATAG

Protein Analysis

535

Amino Acids

58.43

Weight (kDa)

5.28

Isoelectric Point (pI)

33.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_79n PF03662 35 - 350 1.9e-149 Glycosyl hydrolase family 79, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 560
Acc16I TGCGCA 1 cut(s) 1229
Acc65I GGTACC 1 cut(s) 1032
AccB1I GGYRCC 4 cut(s) 305, 739, 1032, 1546
AciI CCGC 3 cut(s) 179, 303, 1339
AclWI GGATC 3 cut(s) 862, 869, 1526
AcoI YGGCCR 1 cut(s) 176
AcsI RAATTY 2 cut(s) 934, 1351
AcuI CTGAAG 2 cut(s) 892, 930
AfaI GTAC 4 cut(s) 44, 810, 1034, 1345
AfeI AGCGCT 1 cut(s) 1159
AfiI CCNNNNNNNGG 4 cut(s) 688, 838, 1428, 1504
AflII CTTAAG 1 cut(s) 929
AhlI ACTAGT 1 cut(s) 730
AjnI CCWGG 3 cut(s) 510, 742, 1035
AleI CACNNNNGTG 1 cut(s) 1541
AluBI AGCT 7 cut(s) 125, 210, 604, 722, 1045, 1206, 1503
AluI AGCT 7 cut(s) 125, 210, 604, 722, 1045, 1206, 1503
Alw21I GWGCWC 2 cut(s) 10, 92
AlwI GGATC 3 cut(s) 862, 869, 1526
AlwNI CAGNNNCTG 2 cut(s) 713, 1029
Aor51HI AGCGCT 1 cut(s) 1159
AoxI GGCC 3 cut(s) 176, 1111, 1363
ApeKI GCWGC 5 cut(s) 125, 518, 1055, 1273, 1335
ApoI RAATTY 2 cut(s) 934, 1351
ArsI GACNNNNNNTTYG 4 cut(s) 92, 124, 134, 166
Asp718I GGTACC 1 cut(s) 1032
AspLEI GCGC 2 cut(s) 1160, 1230
AspS9I GGNCC 3 cut(s) 498, 836, 1039
AsuC2I CCSGG 2 cut(s) 840, 1213
AsuHPI GGTGA 3 cut(s) 273, 539, 977
AvaII GGWCC 3 cut(s) 498, 836, 1039
BaeGI GKGCMC 1 cut(s) 850
BanI GGYRCC 4 cut(s) 305, 739, 1032, 1546
BbsI GAAGAC 3 cut(s) 105, 321, 345
Bbv12I GWGCWC 2 cut(s) 10, 92
BbvI GCAGC 5 cut(s) 112, 505, 1067, 1260, 1347
BccI CCATC 6 cut(s) 395, 417, 884, 886, 1045, 1421
BciT130I CCWGG 3 cut(s) 512, 744, 1037
BciVI GTATCC 1 cut(s) 575
BcnI CCSGG 2 cut(s) 840, 1213
BcuI ACTAGT 1 cut(s) 730
BfaI CTAG 4 cut(s) 236, 420, 605, 731
BfmI CTRYAG 1 cut(s) 1299
BfoI RGCGCY 1 cut(s) 1161
BfrI CTTAAG 1 cut(s) 929
BfuI GTATCC 1 cut(s) 575
BglII AGATCT 1 cut(s) 853
BisI GCNGC 8 cut(s) 126, 179, 304, 519, 1056, 1274, 1336, 1339
BlpI GCTNAGC 1 cut(s) 723
BlsI GCNGC 8 cut(s) 127, 180, 305, 520, 1057, 1275, 1337, 1340
BmcAI AGTACT 2 cut(s) 810, 1345
Bme1390I CCNGG 5 cut(s) 512, 744, 840, 1037, 1213
Bme18I GGWCC 3 cut(s) 498, 836, 1039
BmgT120I GGNCC 3 cut(s) 498, 836, 1039
BmiI GGNNCC 5 cut(s) 307, 741, 1034, 1509, 1548
BmrFI CCNGG 5 cut(s) 512, 744, 840, 1037, 1213
BmsI GCATC 2 cut(s) 1067, 1180
BpiI GAAGAC 3 cut(s) 105, 321, 345
Bpu1102I GCTNAGC 1 cut(s) 723
BpuEI CTTGAG 1 cut(s) 1352
BpuMI CCSGG 2 cut(s) 840, 1213
BsaJI CCNNGG 4 cut(s) 241, 510, 511, 832
Bsc4I CCNNNNNNNGG 4 cut(s) 688, 838, 1428, 1504
Bse1I ACTGG 1 cut(s) 762
Bse3DI GCAATG 1 cut(s) 223
BseBI CCWGG 3 cut(s) 512, 744, 1037
BseDI CCNNGG 4 cut(s) 241, 510, 511, 832
BseGI GGATG 3 cut(s) 433, 1056, 1530
BseLI CCNNNNNNNGG 4 cut(s) 688, 838, 1428, 1504
BseMI GCAATG 1 cut(s) 223
BseMII CTCAG 2 cut(s) 786, 809
BseNI ACTGG 1 cut(s) 762
BseSI GKGCMC 1 cut(s) 850
BseXI GCAGC 5 cut(s) 112, 505, 1067, 1260, 1347
BseYI CCCAGC 3 cut(s) 210, 298, 455
BshFI GGCC 3 cut(s) 178, 1113, 1365
BshNI GGYRCC 4 cut(s) 305, 739, 1032, 1546
BsiHKAI GWGCWC 2 cut(s) 10, 92
BsiSI CCGG 3 cut(s) 182, 839, 1212
BslFI GGGAC 3 cut(s) 1228, 1445, 1574
BslI CCNNNNNNNGG 4 cut(s) 688, 838, 1428, 1504
BsmFI GGGAC 3 cut(s) 1228, 1445, 1574
BsmI GAATGC 3 cut(s) 268, 457, 541
BsnI GGCC 3 cut(s) 178, 1113, 1365
Bsp1286I GDGCHC 3 cut(s) 10, 92, 850
Bsp143I GATC 5 cut(s) 69, 853, 867, 874, 1531
Bsp1720I GCTNAGC 1 cut(s) 723
BspACI CCGC 3 cut(s) 179, 303, 1339
BspANI GGCC 3 cut(s) 178, 1113, 1365
BspCNI CTCAG 2 cut(s) 785, 808
BspLI GGNNCC 5 cut(s) 307, 741, 1034, 1509, 1548
BspPI GGATC 3 cut(s) 862, 869, 1526
BspQI GCTCTTC 1 cut(s) 1167
BspT107I GGYRCC 4 cut(s) 305, 739, 1032, 1546
BspTI CTTAAG 1 cut(s) 929
BsrDI GCAATG 1 cut(s) 223
BsrI ACTGG 1 cut(s) 762
BssECI CCNNGG 4 cut(s) 241, 510, 511, 832
BssMI GATC 5 cut(s) 69, 853, 867, 874, 1531
BssT1I CCWWGG 2 cut(s) 241, 832
Bst2UI CCWGG 3 cut(s) 512, 744, 1037
Bst6I CTCTTC 3 cut(s) 26, 231, 1167
BstAFI CTTAAG 1 cut(s) 929
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 6 cut(s) 293, 310, 723, 772, 795, 1285
BstF5I GGATG 3 cut(s) 433, 1056, 1530
BstH2I RGCGCY 1 cut(s) 1161
BstHHI GCGC 2 cut(s) 1160, 1230
BstKTI GATC 5 cut(s) 72, 856, 870, 877, 1534
BstMBI GATC 5 cut(s) 69, 853, 867, 874, 1531
BstMWI GCNNNNNNNGC 2 cut(s) 272, 545
BstNI CCWGG 3 cut(s) 512, 744, 1037
BstSCI CCNGG 5 cut(s) 510, 742, 838, 1035, 1211
BstSFI CTRYAG 1 cut(s) 1299
BstSLI GKGCMC 1 cut(s) 850
BstV1I GCAGC 5 cut(s) 112, 505, 1067, 1260, 1347
BstV2I GAAGAC 3 cut(s) 105, 321, 345
BstX2I RGATCY 3 cut(s) 853, 867, 1531
BstXI CCANNNNNNTGG 1 cut(s) 1543
BstYI RGATCY 3 cut(s) 853, 867, 1531
BsuI GTATCC 1 cut(s) 575
BsuRI GGCC 3 cut(s) 178, 1113, 1365
BtsCI GGATG 3 cut(s) 433, 1056, 1530
BtsI GCAGTG 2 cut(s) 485, 957
BtsIMutI CAGTG 6 cut(s) 102, 485, 916, 957, 994, 1275
Cac8I GCNNGC 1 cut(s) 6
CaiI CAGNNNCTG 2 cut(s) 713, 1029
CfoI GCGC 2 cut(s) 1160, 1230
Cfr13I GGNCC 3 cut(s) 498, 836, 1039
Csp6I GTAC 4 cut(s) 43, 809, 1033, 1344
CspCI CAANNNNNGTGG 2 cut(s) 717, 752
CviAII CATG 2 cut(s) 593, 957
CviQI GTAC 4 cut(s) 43, 809, 1033, 1344
DdeI CTNAG 6 cut(s) 293, 310, 723, 772, 795, 1285
DpnI GATC 5 cut(s) 71, 855, 869, 876, 1533
DpnII GATC 5 cut(s) 69, 853, 867, 874, 1531
EaeI YGGCCR 1 cut(s) 176
Eam1104I CTCTTC 3 cut(s) 26, 231, 1167
EarI CTCTTC 3 cut(s) 26, 231, 1167
Eco130I CCWWGG 2 cut(s) 241, 832
Eco147I AGGCCT 1 cut(s) 1365
Eco47I GGWCC 3 cut(s) 498, 836, 1039
Eco47III AGCGCT 1 cut(s) 1159
Eco57I CTGAAG 2 cut(s) 892, 930
EcoRI GAATTC 1 cut(s) 934
EcoRII CCWGG 3 cut(s) 510, 742, 1035
EcoT14I CCWWGG 2 cut(s) 241, 832
EcoT22I ATGCAT 1 cut(s) 1015
ErhI CCWWGG 2 cut(s) 241, 832
FaeI CATG 2 cut(s) 596, 960
FaqI GGGAC 3 cut(s) 1228, 1445, 1574
FatI CATG 2 cut(s) 592, 956
Fnu4HI GCNGC 8 cut(s) 126, 179, 304, 519, 1056, 1274, 1336, 1339
FokI GGATG 3 cut(s) 440, 1063, 1537
Fsp4HI GCNGC 8 cut(s) 126, 179, 304, 519, 1056, 1274, 1336, 1339
FspBI CTAG 4 cut(s) 236, 420, 605, 731
FspI TGCGCA 1 cut(s) 1229
GlaI GCGC 2 cut(s) 1159, 1229
GluI GCNGC 8 cut(s) 126, 179, 304, 519, 1056, 1274, 1336, 1339
GsaI CCCAGC 3 cut(s) 214, 302, 459
HaeII RGCGCY 1 cut(s) 1161
HaeIII GGCC 3 cut(s) 178, 1113, 1365
HapII CCGG 3 cut(s) 182, 839, 1212
HhaI GCGC 2 cut(s) 1160, 1230
Hin1II CATG 2 cut(s) 596, 960
Hin6I GCGC 2 cut(s) 1158, 1228
HinP1I GCGC 2 cut(s) 1158, 1228
HincII GTYRAC 3 cut(s) 574, 862, 1200
HindII GTYRAC 3 cut(s) 574, 862, 1200
HindIII AAGCTT 2 cut(s) 1204, 1501
HinfI GANTC 8 cut(s) 506, 551, 642, 758, 968, 1263, 1373, 1481
HpaII CCGG 3 cut(s) 182, 839, 1212
HphI GGTGA 3 cut(s) 273, 539, 977
Hpy166II GTNNAC 5 cut(s) 380, 574, 862, 1200, 1570
Hpy188I TCNGA 6 cut(s) 134, 400, 667, 715, 775, 1531
Hpy188III TCNNGA 5 cut(s) 236, 448, 887, 972, 1145
Hpy8I GTNNAC 5 cut(s) 380, 574, 862, 1200, 1570
HpyAV CCTTC 1 cut(s) 916
HpyCH4V TGCA 8 cut(s) 128, 266, 485, 490, 694, 1013, 1083, 1438
HpyF10VI GCNNNNNNNGC 2 cut(s) 272, 545
HpyF3I CTNAG 6 cut(s) 293, 310, 723, 772, 795, 1285
Hsp92II CATG 2 cut(s) 596, 960
HspAI GCGC 2 cut(s) 1158, 1228
KpnI GGTACC 1 cut(s) 1036
Kzo9I GATC 5 cut(s) 69, 853, 867, 874, 1531
LguI GCTCTTC 1 cut(s) 1167
LmnI GCTCC 2 cut(s) 601, 1507
Lsp1109I GCAGC 5 cut(s) 112, 505, 1067, 1260, 1347
LweI GCATC 2 cut(s) 1067, 1180
MaeI CTAG 4 cut(s) 236, 420, 605, 731
MaeIII GTNAC 7 cut(s) 49, 404, 643, 911, 992, 1003, 1292
MalI GATC 5 cut(s) 71, 855, 869, 876, 1533
MboI GATC 5 cut(s) 69, 853, 867, 874, 1531
MboII GAAGA 9 cut(s) 8, 13, 16, 110, 218, 326, 350, 1154, 1415
MflI RGATCY 3 cut(s) 853, 867, 1531
MhlI GDGCHC 3 cut(s) 10, 92, 850
MluCI AATT 5 cut(s) 588, 716, 934, 1105, 1351
MlyI GAGTC 1 cut(s) 651
MmeI TCCRAC 1 cut(s) 613
MnlI CCTC 8 cut(s) 88, 740, 804, 881, 968, 1124, 1376, 1549
Mph1103I ATGCAT 1 cut(s) 1015
MseI TTAA 3 cut(s) 231, 930, 1415
MslI CAYNNNNRTG 1 cut(s) 1541
MspA1I CMGCKG 1 cut(s) 125
MspCI CTTAAG 1 cut(s) 929
MspI CCGG 3 cut(s) 182, 839, 1212
MspR9I CCNGG 5 cut(s) 512, 744, 840, 1037, 1213
Mva1269I GAATGC 3 cut(s) 268, 457, 541
MvaI CCWGG 3 cut(s) 512, 744, 1037
MwoI GCNNNNNNNGC 2 cut(s) 272, 545
NciI CCSGG 2 cut(s) 840, 1213
NdeII GATC 5 cut(s) 69, 853, 867, 874, 1531
NlaIII CATG 2 cut(s) 596, 960
NlaIV GGNNCC 5 cut(s) 307, 741, 1034, 1509, 1548
NmeAIII GCCGAG 1 cut(s) 1469
NmuCI GTSAC 4 cut(s) 49, 643, 911, 992
NsbI TGCGCA 1 cut(s) 1229
NsiI ATGCAT 1 cut(s) 1015
OliI CACNNNNGTG 1 cut(s) 1541
PasI CCCWGGG 1 cut(s) 511
PceI AGGCCT 1 cut(s) 1365
PciSI GCTCTTC 1 cut(s) 1167
PctI GAATGC 3 cut(s) 268, 457, 541
PfeI GAWTC 7 cut(s) 506, 551, 758, 968, 1263, 1373, 1481
PflFI GACNNNGTC 2 cut(s) 104, 320
PfoI TCCNGGA 1 cut(s) 1211
PkrI GCNGC 8 cut(s) 127, 180, 305, 520, 1057, 1275, 1337, 1340
PleI GAGTC 1 cut(s) 650
PpsI GAGTC 1 cut(s) 650
PsiI TTATAA 1 cut(s) 560
Psp6I CCWGG 3 cut(s) 510, 742, 1035
PspFI CCCAGC 3 cut(s) 210, 298, 455
PspGI CCWGG 3 cut(s) 510, 742, 1035
PspN4I GGNNCC 5 cut(s) 307, 741, 1034, 1509, 1548
PspPI GGNCC 3 cut(s) 498, 836, 1039
PstNI CAGNNNCTG 2 cut(s) 713, 1029
PsuI RGATCY 3 cut(s) 853, 867, 1531
PsyI GACNNNGTC 2 cut(s) 104, 320
PvuII CAGCTG 1 cut(s) 125
RsaI GTAC 4 cut(s) 44, 810, 1034, 1345
RsaNI GTAC 4 cut(s) 43, 809, 1033, 1344
RseI CAYNNNNRTG 1 cut(s) 1541
SapI GCTCTTC 1 cut(s) 1167
SaqAI TTAA 3 cut(s) 231, 930, 1415
SatI GCNGC 8 cut(s) 126, 179, 304, 519, 1056, 1274, 1336, 1339
Sau3AI GATC 5 cut(s) 69, 853, 867, 874, 1531
Sau96I GGNCC 3 cut(s) 498, 836, 1039
ScaI AGTACT 2 cut(s) 810, 1345
SchI GAGTC 1 cut(s) 651
ScrFI CCNGG 5 cut(s) 512, 744, 840, 1037, 1213
SduI GDGCHC 3 cut(s) 10, 92, 850
SfaNI GCATC 2 cut(s) 1067, 1180
SfcI CTRYAG 1 cut(s) 1299
SinI GGWCC 3 cut(s) 498, 836, 1039
SmiMI CAYNNNNRTG 1 cut(s) 1541
SmlI CTYRAG 2 cut(s) 929, 1367
SmoI CTYRAG 2 cut(s) 929, 1367
SpeI ACTAGT 1 cut(s) 730
Sse9I AATT 5 cut(s) 588, 716, 934, 1105, 1351
SseBI AGGCCT 1 cut(s) 1365
SsiI CCGC 3 cut(s) 179, 303, 1339
SspMI CTAG 4 cut(s) 236, 420, 605, 731
StuI AGGCCT 1 cut(s) 1365
StyD4I CCNGG 5 cut(s) 510, 742, 838, 1035, 1211
StyI CCWWGG 2 cut(s) 241, 832
TaqI TCGA 3 cut(s) 68, 873, 1311
TasI AATT 5 cut(s) 588, 716, 934, 1105, 1351
TatI WGTACW 2 cut(s) 808, 1343
TauI GCSGC 3 cut(s) 181, 306, 1341
TfiI GAWTC 7 cut(s) 506, 551, 758, 968, 1263, 1373, 1481
Tru1I TTAA 3 cut(s) 231, 930, 1415
Tru9I TTAA 3 cut(s) 231, 930, 1415
TscAI CASTG 6 cut(s) 109, 492, 916, 957, 994, 1275
TseFI GTSAC 4 cut(s) 49, 643, 911, 992
TseI GCWGC 5 cut(s) 125, 518, 1055, 1273, 1335
Tsp45I GTSAC 4 cut(s) 49, 643, 911, 992
TspDTI ATGAA 4 cut(s) 351, 444, 581, 627
TspGWI ACGGA 1 cut(s) 107
TspRI CASTG 6 cut(s) 109, 492, 916, 957, 994, 1275
Tth111I GACNNNGTC 2 cut(s) 104, 320
Vha464I CTTAAG 1 cut(s) 929
VpaK11BI GGWCC 3 cut(s) 498, 836, 1039
XapI RAATTY 2 cut(s) 934, 1351
XbaI TCTAGA 1 cut(s) 235
XspI CTAG 4 cut(s) 236, 420, 605, 731
ZrmI AGTACT 2 cut(s) 810, 1345
Zsp2I ATGCAT 1 cut(s) 1015
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.