Rh6BG103300

heparanase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001523
Physical Location & Seq
Forward (+)
15983178 .. 16002380
19203 bp
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UTR
Exon/CDS
Intron
Rh6BG103300.1

Sequence Viewer

Length: 408 bp
ATGTTAGAAGAAGGAAAGGGAATAGGAGGAGGTAGTACTGAAGGTTTGGTTAGAATTGATGGGAAAGAAACCATTGGGAAAATTGATGAAGATTTTGTTTGTGCAACTTTGGATTGGTGGCCTCCTGAGAAATGTGACTATGGAACTTGCAGCTGGGGCAAGGCTACTCTTCTTAATCTGGATCTAAGTAATACTGTCTTATCAAAGGCCATAAAGGGAAATGAGCTATGTGGGCGTGGAATTGGAACCACAGTAGCAGCAAGTGCTAGGCAGCAAAAGGTGAAGTGTTCGGTGAATGTGAAAGATTTTCCAAATATTATCGTTCGCTTAGCCCAGGTGAATGGAAGGAGAGAGGTGAACAAGAAAGTGCTTAAAGTTCCTGGAATTGAACAGAAGGAGAACTTGTAA

Protein Analysis

135

Amino Acids

14.63

Weight (kDa)

8.61

Isoelectric Point (pI)

22.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_79n PF03662 15 - 72 1.6e-25 Glycosyl hydrolase family 79, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 189
AcuI CTGAAG 1 cut(s) 60
AfaI GTAC 1 cut(s) 37
AgsI TTSAA 1 cut(s) 389
AjnI CCWGG 2 cut(s) 333, 379
AluBI AGCT 2 cut(s) 153, 226
AluI AGCT 2 cut(s) 153, 226
AlwI GGATC 1 cut(s) 189
AoxI GGCC 2 cut(s) 119, 207
ApeKI GCWGC 3 cut(s) 150, 257, 271
AsuHPI GGTGA 4 cut(s) 292, 304, 349, 367
BbvI GCAGC 3 cut(s) 162, 269, 283
BccI CCATC 1 cut(s) 53
BciT130I CCWGG 2 cut(s) 335, 381
BfaI CTAG 1 cut(s) 267
BisI GCNGC 3 cut(s) 151, 258, 272
BlpI GCTNAGC 1 cut(s) 328
BlsI GCNGC 3 cut(s) 152, 259, 273
BmcAI AGTACT 1 cut(s) 37
Bme1390I CCNGG 2 cut(s) 335, 381
BmiI GGNNCC 1 cut(s) 247
BmrFI CCNGG 2 cut(s) 335, 381
Bpu1102I GCTNAGC 1 cut(s) 328
BsaJI CCNNGG 1 cut(s) 333
BsaXI ACNNNNNCTCC 1 cut(s) 389
BseBI CCWGG 2 cut(s) 335, 381
BseDI CCNNGG 1 cut(s) 333
BseMII CTCAG 1 cut(s) 117
BseRI GAGGAG 1 cut(s) 42
BseXI GCAGC 3 cut(s) 162, 269, 283
BseYI CCCAGC 1 cut(s) 153
BshFI GGCC 2 cut(s) 121, 209
BsnI GGCC 2 cut(s) 121, 209
Bsp143I GATC 1 cut(s) 181
Bsp1720I GCTNAGC 1 cut(s) 328
BspANI GGCC 2 cut(s) 121, 209
BspCNI CTCAG 1 cut(s) 118
BspLI GGNNCC 1 cut(s) 247
BspPI GGATC 1 cut(s) 189
BssECI CCNNGG 1 cut(s) 333
BssMI GATC 1 cut(s) 181
Bst2UI CCWGG 2 cut(s) 335, 381
Bst4CI ACNGT 2 cut(s) 196, 253
Bst6I CTCTTC 1 cut(s) 174
BstAPI GCANNNNNTGC 1 cut(s) 263
BstDEI CTNAG 3 cut(s) 126, 185, 328
BstKTI GATC 1 cut(s) 184
BstMBI GATC 1 cut(s) 181
BstMWI GCNNNNNNNGC 3 cut(s) 156, 232, 263
BstNI CCWGG 2 cut(s) 335, 381
BstSCI CCNGG 2 cut(s) 333, 379
BstV1I GCAGC 3 cut(s) 162, 269, 283
BstX2I RGATCY 1 cut(s) 181
BstXI CCANNNNNNTGG 1 cut(s) 341
BstYI RGATCY 1 cut(s) 181
BsuRI GGCC 2 cut(s) 121, 209
Csp6I GTAC 1 cut(s) 36
CviJI RGCY 6 cut(s) 121, 153, 164, 209, 226, 332
CviKI_1 RGCY 6 cut(s) 121, 153, 164, 209, 226, 332
CviQI GTAC 1 cut(s) 36
DdeI CTNAG 3 cut(s) 126, 185, 328
DpnI GATC 1 cut(s) 183
DpnII GATC 1 cut(s) 181
Eam1104I CTCTTC 1 cut(s) 174
EarI CTCTTC 1 cut(s) 174
Eco57I CTGAAG 1 cut(s) 60
EcoRII CCWGG 2 cut(s) 333, 379
FaiI YATR 3 cut(s) 141, 212, 229
FalI AAGNNNNNCTT 1 cut(s) 386
Fnu4HI GCNGC 3 cut(s) 151, 258, 272
Fsp4HI GCNGC 3 cut(s) 151, 258, 272
FspBI CTAG 1 cut(s) 267
GluI GCNGC 3 cut(s) 151, 258, 272
GsaI CCCAGC 1 cut(s) 157
HaeIII GGCC 2 cut(s) 121, 209
HphI GGTGA 4 cut(s) 292, 304, 349, 367
Hpy166II GTNNAC 1 cut(s) 358
Hpy188III TCNNGA 2 cut(s) 125, 179
Hpy8I GTNNAC 1 cut(s) 358
HpyAV CCTTC 4 cut(s) 5, 35, 339, 388
HpyCH4III ACNGT 2 cut(s) 196, 253
HpyCH4V TGCA 2 cut(s) 104, 150
HpyF10VI GCNNNNNNNGC 3 cut(s) 156, 232, 263
HpyF3I CTNAG 3 cut(s) 126, 185, 328
Kzo9I GATC 1 cut(s) 181
LpnPI CCDG 7 cut(s) 138, 139, 164, 320, 347, 366, 393
Lsp1109I GCAGC 3 cut(s) 162, 269, 283
MaeI CTAG 1 cut(s) 267
MaeIII GTNAC 1 cut(s) 134
MalI GATC 1 cut(s) 183
MboI GATC 1 cut(s) 181
MboII GAAGA 3 cut(s) 20, 101, 161
MflI RGATCY 1 cut(s) 181
MluCI AATT 4 cut(s) 54, 81, 240, 384
MnlI CCTC 4 cut(s) 20, 23, 132, 346
MseI TTAA 2 cut(s) 174, 372
MspA1I CMGCKG 1 cut(s) 153
MspR9I CCNGG 2 cut(s) 335, 381
MvaI CCWGG 2 cut(s) 335, 381
MwoI GCNNNNNNNGC 3 cut(s) 156, 232, 263
NdeII GATC 1 cut(s) 181
NlaIV GGNNCC 1 cut(s) 247
NmuCI GTSAC 1 cut(s) 134
PfoI TCCNGGA 1 cut(s) 379
PkrI GCNGC 3 cut(s) 152, 259, 273
Psp6I CCWGG 2 cut(s) 333, 379
PspFI CCCAGC 1 cut(s) 153
PspGI CCWGG 2 cut(s) 333, 379
PspN4I GGNNCC 1 cut(s) 247
PsuI RGATCY 1 cut(s) 181
PvuII CAGCTG 1 cut(s) 153
RsaI GTAC 1 cut(s) 37
RsaNI GTAC 1 cut(s) 36
SaqAI TTAA 2 cut(s) 174, 372
SatI GCNGC 3 cut(s) 151, 258, 272
Sau3AI GATC 1 cut(s) 181
ScaI AGTACT 1 cut(s) 37
ScrFI CCNGG 2 cut(s) 335, 381
SetI ASST 7 cut(s) 34, 46, 155, 228, 282, 339, 357
Sse9I AATT 4 cut(s) 54, 81, 240, 384
SspI AATATT 1 cut(s) 316
SspMI CTAG 1 cut(s) 267
StyD4I CCNGG 2 cut(s) 333, 379
TaaI ACNGT 2 cut(s) 196, 253
TasI AATT 4 cut(s) 54, 81, 240, 384
TatI WGTACW 1 cut(s) 35
Tru1I TTAA 2 cut(s) 174, 372
Tru9I TTAA 2 cut(s) 174, 372
TseFI GTSAC 1 cut(s) 134
TseI GCWGC 3 cut(s) 150, 257, 271
Tsp45I GTSAC 1 cut(s) 134
TspDTI ATGAA 1 cut(s) 102
XspI CTAG 1 cut(s) 267
ZrmI AGTACT 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.