Rh6BG392100

heparanase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001523
Physical Location & Seq
Forward (+)
62071574 .. 62075001
3428 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG392100.1

Sequence Viewer

Length: 1407 bp
ATGTGTGAAATAATGCGTGCTCTGGTTCTTCTCTTCTTCGTTTGCTTCTGGCTGTACGATGTCACTGACAAACACAACTCGATCTCTGTGGCTTCACAGAGCACGGAGGAAGACACTGTCTTTGTCAATGGAACAGCTGCAATCGGAAGCATAGACGATGACTTTGTTTGTGCTACAATGGATTGGTGGCCGCCGGAGAAATGCGACTATGGAACTTGTAGCTGGGGCAATGCTTCTCTTCTTAATGTAGACCTTGGCAACGATATACTTTTGAATGCAATAAAAGCATTTTCACCCTTGAAACTTAGGCTGGGCGGCACCTTACAAGACAAAGTCATATACCAGACAGAAGACAGTCCAAAACATCATACTTCATTTGTCAAAGATAGTTCACAGTTGTTTGGCTTCTCTGATGGTTACTTACCTATGCCTAGATGGGATGAACTGAACATTTTGTTCAAGAATGCTGGGGCTGTTGTTATATTTGGACTAAATGCACTGCAAGGCAGGACCATAGATTCCCATGGTGCTGCTGTGGGTGATTGGAATGCGAGTAATGCTGAATCTTTTATAAGATATACAGTCAACAAAGGATACACAATTCATGGTTGGGAGCTAGGAAATGAACTATGTGGGAGTGGAGTTGGAACAAGAGTCACAGCAGACCAATACGCTTCGGACATAAACTCTCTCCATAACAAGGTGCAAGATATTTATTCAGGTTCTGAATTAGCTAAGCCACTAGTCTTGGCACCAGGAGGGTTCTTTGATTCCAGTTGGTTCTCAGATTTTGTTGATAAAGCACCTCAGTCTGTTCAAGTACTCACACACCACATATACAACCTTGGTCCAGGTAGTGCCCCGGATCTTGTTGACAAGATCCTCGATCCATCTGTTCTTGATGGTATTTCCAAAACCTTCAGTGACCTACAAATGCTACTTAAGAATTCAAAAACTTCAGCAGTGGCATGGGTTGGTGAATCAGGAGGTGCTTACAACAGTGGTCACAATCTTGTTACAAATGCATTTGTATTCAGTTTCTGGTACCTGGACCAGCTTGGGATGGCAGCATCTTATGATACGAAAACATATTGCAGACAAACACTCATTGGTGGAAATTATGGCCTCCTTGACATCCAAACCTTTCAACCAAATCCTGATTATTACAGCGCTCTTCTTTGGCATCGTCTAATGGGAAACAATGTCCTGTCAACAAGCTTTTCCGGGACAAAGAAACTGCGCACTTACTCTCATTGTTCCAAGAAAACAGTAAGTAAAAGAATTGCACAAAAACTCACTTGTAATGAAGGAAAATGTTATTTTGGTATATGTTCGGGATGTTATGTTGCTAAACTATCAATAATTGAATATATATACTCTTGTTTGAGATCTCATCTAGCAACATAA

Protein Analysis

468

Amino Acids

51.59

Weight (kDa)

5.72

Isoelectric Point (pI)

33.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_79n PF03662 39 - 354 1.5e-150 Glycosyl hydrolase family 79, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 572
Acc16I TGCGCA 1 cut(s) 1241
Acc65I GGTACC 1 cut(s) 1044
AccB1I GGYRCC 3 cut(s) 317, 751, 1044
AccI GTMKAC 1 cut(s) 249
AciI CCGC 2 cut(s) 191, 315
AclWI GGATC 3 cut(s) 873, 874, 881
AcoI YGGCCR 1 cut(s) 188
AcsI RAATTY 1 cut(s) 946
AcuI CTGAAG 2 cut(s) 904, 942
AfaI GTAC 3 cut(s) 56, 822, 1046
AfeI AGCGCT 1 cut(s) 1171
AfiI CCNNNNNNNGG 1 cut(s) 700
AflII CTTAAG 1 cut(s) 941
AgsI TTSAA 7 cut(s) 274, 301, 460, 818, 951, 1148, 1367
AhlI ACTAGT 1 cut(s) 742
AjnI CCWGG 3 cut(s) 754, 850, 1047
AluBI AGCT 6 cut(s) 137, 222, 616, 734, 1057, 1218
AluI AGCT 6 cut(s) 137, 222, 616, 734, 1057, 1218
Alw21I GWGCWC 2 cut(s) 22, 104
AlwI GGATC 3 cut(s) 873, 874, 881
AlwNI CAGNNNCTG 2 cut(s) 725, 1041
Aor51HI AGCGCT 1 cut(s) 1171
AoxI GGCC 2 cut(s) 188, 1123
ApeKI GCWGC 3 cut(s) 137, 530, 1067
ApoI RAATTY 1 cut(s) 946
ArsI GACNNNNNNTTYG 4 cut(s) 104, 136, 146, 178
Asp718I GGTACC 1 cut(s) 1044
AspLEI GCGC 2 cut(s) 1172, 1242
AspS9I GGNCC 3 cut(s) 510, 848, 1051
AsuC2I CCSGG 2 cut(s) 863, 1225
AsuHPI GGTGA 3 cut(s) 285, 551, 989
AvaII GGWCC 3 cut(s) 510, 848, 1051
BaeGI GKGCMC 1 cut(s) 862
BanI GGYRCC 3 cut(s) 317, 751, 1044
BbsI GAAGAC 2 cut(s) 117, 357
Bbv12I GWGCWC 2 cut(s) 22, 104
BbvI GCAGC 3 cut(s) 124, 517, 1079
BccI CCATC 5 cut(s) 407, 429, 896, 898, 1057
BciT130I CCWGG 3 cut(s) 756, 852, 1049
BciVI GTATCC 1 cut(s) 587
BcnI CCSGG 2 cut(s) 863, 1225
BcuI ACTAGT 1 cut(s) 742
BfaI CTAG 4 cut(s) 432, 617, 743, 1397
BfoI RGCGCY 1 cut(s) 1173
BfrI CTTAAG 1 cut(s) 941
BfuI GTATCC 1 cut(s) 587
BglII AGATCT 1 cut(s) 1388
BisI GCNGC 5 cut(s) 138, 191, 316, 531, 1068
BlpI GCTNAGC 1 cut(s) 735
BlsI GCNGC 5 cut(s) 139, 192, 317, 532, 1069
BmcAI AGTACT 1 cut(s) 822
Bme1390I CCNGG 5 cut(s) 756, 852, 863, 1049, 1225
Bme18I GGWCC 3 cut(s) 510, 848, 1051
BmgT120I GGNCC 3 cut(s) 510, 848, 1051
BmiI GGNNCC 3 cut(s) 319, 753, 1046
BmrFI CCNGG 5 cut(s) 756, 852, 863, 1049, 1225
BmsI GCATC 2 cut(s) 1079, 1192
BpiI GAAGAC 2 cut(s) 117, 357
Bpu1102I GCTNAGC 1 cut(s) 735
BpuMI CCSGG 2 cut(s) 863, 1225
BsaJI CCNNGG 4 cut(s) 253, 523, 844, 861
Bsc4I CCNNNNNNNGG 1 cut(s) 700
Bse1I ACTGG 1 cut(s) 774
Bse3DI GCAATG 1 cut(s) 235
BseBI CCWGG 3 cut(s) 756, 852, 1049
BseDI CCNNGG 4 cut(s) 253, 523, 844, 861
BseGI GGATG 4 cut(s) 445, 1068, 1134, 1343
BseLI CCNNNNNNNGG 1 cut(s) 700
BseMI GCAATG 1 cut(s) 235
BseMII CTCAG 2 cut(s) 798, 821
BseNI ACTGG 1 cut(s) 774
BseSI GKGCMC 1 cut(s) 862
BseXI GCAGC 3 cut(s) 124, 517, 1079
BseYI CCCAGC 3 cut(s) 222, 310, 467
BshFI GGCC 2 cut(s) 190, 1125
BshNI GGYRCC 3 cut(s) 317, 751, 1044
BsiHKAI GWGCWC 2 cut(s) 22, 104
BsiSI CCGG 3 cut(s) 194, 863, 1224
BslFI GGGAC 1 cut(s) 1240
BslI CCNNNNNNNGG 1 cut(s) 700
BsmFI GGGAC 1 cut(s) 1240
BsmI GAATGC 3 cut(s) 280, 469, 553
BsnI GGCC 2 cut(s) 190, 1125
Bsp1286I GDGCHC 3 cut(s) 22, 104, 862
Bsp143I GATC 5 cut(s) 81, 865, 879, 886, 1388
Bsp1720I GCTNAGC 1 cut(s) 735
Bsp19I CCATGG 1 cut(s) 523
BspACI CCGC 2 cut(s) 191, 315
BspANI GGCC 2 cut(s) 190, 1125
BspCNI CTCAG 2 cut(s) 797, 820
BspLI GGNNCC 3 cut(s) 319, 753, 1046
BspPI GGATC 3 cut(s) 873, 874, 881
BspQI GCTCTTC 1 cut(s) 1179
BspT107I GGYRCC 3 cut(s) 317, 751, 1044
BspTI CTTAAG 1 cut(s) 941
BsrDI GCAATG 1 cut(s) 235
BsrI ACTGG 1 cut(s) 774
BssECI CCNNGG 4 cut(s) 253, 523, 844, 861
BssMI GATC 5 cut(s) 81, 865, 879, 886, 1388
BssT1I CCWWGG 3 cut(s) 253, 523, 844
Bst2UI CCWGG 3 cut(s) 756, 852, 1049
Bst4CI ACNGT 6 cut(s) 118, 356, 396, 583, 1001, 1270
Bst6I CTCTTC 3 cut(s) 38, 243, 1179
BstAFI CTTAAG 1 cut(s) 941
BstC8I GCNNGC 1 cut(s) 18
BstDEI CTNAG 4 cut(s) 305, 735, 784, 807
BstDSI CCRYGG 1 cut(s) 523
BstF5I GGATG 4 cut(s) 445, 1068, 1134, 1343
BstH2I RGCGCY 1 cut(s) 1173
BstHHI GCGC 2 cut(s) 1172, 1242
BstKTI GATC 5 cut(s) 84, 868, 882, 889, 1391
BstMBI GATC 5 cut(s) 81, 865, 879, 886, 1388
BstMWI GCNNNNNNNGC 2 cut(s) 284, 557
BstNI CCWGG 3 cut(s) 756, 852, 1049
BstSCI CCNGG 5 cut(s) 754, 850, 861, 1047, 1223
BstSLI GKGCMC 1 cut(s) 862
BstV1I GCAGC 3 cut(s) 124, 517, 1079
BstV2I GAAGAC 2 cut(s) 117, 357
BstX2I RGATCY 3 cut(s) 865, 879, 1388
BstYI RGATCY 3 cut(s) 865, 879, 1388
BsuI GTATCC 1 cut(s) 587
BsuRI GGCC 2 cut(s) 190, 1125
BtgI CCRYGG 1 cut(s) 523
BtsCI GGATG 4 cut(s) 445, 1068, 1134, 1343
BtsI GCAGTG 2 cut(s) 497, 969
BtsIMutI CAGTG 6 cut(s) 63, 114, 497, 928, 969, 1006
Cac8I GCNNGC 1 cut(s) 18
CaiI CAGNNNCTG 2 cut(s) 725, 1041
CfoI GCGC 2 cut(s) 1172, 1242
Cfr13I GGNCC 3 cut(s) 510, 848, 1051
Csp6I GTAC 3 cut(s) 55, 821, 1045
CspCI CAANNNNNGTGG 2 cut(s) 729, 764
CviAII CATG 3 cut(s) 524, 605, 969
CviQI GTAC 3 cut(s) 55, 821, 1045
DdeI CTNAG 4 cut(s) 305, 735, 784, 807
DpnI GATC 5 cut(s) 83, 867, 881, 888, 1390
DpnII GATC 5 cut(s) 81, 865, 879, 886, 1388
EaeI YGGCCR 1 cut(s) 188
Eam1104I CTCTTC 3 cut(s) 38, 243, 1179
EarI CTCTTC 3 cut(s) 38, 243, 1179
Eco130I CCWWGG 3 cut(s) 253, 523, 844
Eco47I GGWCC 3 cut(s) 510, 848, 1051
Eco47III AGCGCT 1 cut(s) 1171
Eco57I CTGAAG 2 cut(s) 904, 942
EcoRI GAATTC 1 cut(s) 946
EcoRII CCWGG 3 cut(s) 754, 850, 1047
EcoT14I CCWWGG 3 cut(s) 253, 523, 844
EcoT22I ATGCAT 1 cut(s) 1027
ErhI CCWWGG 3 cut(s) 253, 523, 844
FaeI CATG 3 cut(s) 527, 608, 972
FaqI GGGAC 1 cut(s) 1240
FatI CATG 3 cut(s) 523, 604, 968
FblI GTMKAC 1 cut(s) 249
Fnu4HI GCNGC 5 cut(s) 138, 191, 316, 531, 1068
FokI GGATG 4 cut(s) 452, 1075, 1121, 1350
Fsp4HI GCNGC 5 cut(s) 138, 191, 316, 531, 1068
FspBI CTAG 4 cut(s) 432, 617, 743, 1397
FspI TGCGCA 1 cut(s) 1241
GlaI GCGC 2 cut(s) 1171, 1241
GluI GCNGC 5 cut(s) 138, 191, 316, 531, 1068
GsaI CCCAGC 3 cut(s) 226, 314, 471
HaeII RGCGCY 1 cut(s) 1173
HaeIII GGCC 2 cut(s) 190, 1125
HapII CCGG 3 cut(s) 194, 863, 1224
HhaI GCGC 2 cut(s) 1172, 1242
Hin1II CATG 3 cut(s) 527, 608, 972
Hin6I GCGC 2 cut(s) 1170, 1240
HinP1I GCGC 2 cut(s) 1170, 1240
HincII GTYRAC 3 cut(s) 586, 874, 1212
HindII GTYRAC 3 cut(s) 586, 874, 1212
HindIII AAGCTT 1 cut(s) 1216
HinfI GANTC 5 cut(s) 518, 563, 654, 770, 980
HpaII CCGG 3 cut(s) 194, 863, 1224
HphI GGTGA 3 cut(s) 285, 551, 989
Hpy166II GTNNAC 5 cut(s) 250, 392, 586, 874, 1212
Hpy188I TCNGA 5 cut(s) 146, 412, 679, 727, 787
Hpy188III TCNNGA 5 cut(s) 460, 899, 984, 1157, 1335
Hpy8I GTNNAC 5 cut(s) 250, 392, 586, 874, 1212
HpyAV CCTTC 2 cut(s) 928, 1301
HpyCH4III ACNGT 6 cut(s) 118, 356, 396, 583, 1001, 1270
HpyCH4V TGCA 8 cut(s) 140, 278, 497, 502, 706, 1025, 1095, 1286
HpyF10VI GCNNNNNNNGC 2 cut(s) 284, 557
HpyF3I CTNAG 4 cut(s) 305, 735, 784, 807
Hsp92II CATG 3 cut(s) 527, 608, 972
HspAI GCGC 2 cut(s) 1170, 1240
KpnI GGTACC 1 cut(s) 1048
Kzo9I GATC 5 cut(s) 81, 865, 879, 886, 1388
LguI GCTCTTC 1 cut(s) 1179
LmnI GCTCC 1 cut(s) 613
Lsp1109I GCAGC 3 cut(s) 124, 517, 1079
LweI GCATC 2 cut(s) 1079, 1192
MaeI CTAG 4 cut(s) 432, 617, 743, 1397
MaeIII GTNAC 6 cut(s) 61, 416, 655, 923, 1004, 1015
MalI GATC 5 cut(s) 83, 867, 881, 888, 1390
MboI GATC 5 cut(s) 81, 865, 879, 886, 1388
MboII GAAGA 7 cut(s) 20, 25, 28, 122, 230, 362, 1166
MflI RGATCY 3 cut(s) 865, 879, 1388
MhlI GDGCHC 3 cut(s) 22, 104, 862
MluCI AATT 6 cut(s) 600, 728, 946, 1117, 1281, 1362
MlyI GAGTC 1 cut(s) 663
MmeI TCCRAC 1 cut(s) 625
MnlI CCTC 6 cut(s) 100, 752, 816, 893, 980, 1136
Mph1103I ATGCAT 1 cut(s) 1027
MseI TTAA 2 cut(s) 243, 942
MspA1I CMGCKG 1 cut(s) 137
MspCI CTTAAG 1 cut(s) 941
MspI CCGG 3 cut(s) 194, 863, 1224
MspR9I CCNGG 5 cut(s) 756, 852, 863, 1049, 1225
Mva1269I GAATGC 3 cut(s) 280, 469, 553
MvaI CCWGG 3 cut(s) 756, 852, 1049
MwoI GCNNNNNNNGC 2 cut(s) 284, 557
NciI CCSGG 2 cut(s) 863, 1225
NcoI CCATGG 1 cut(s) 523
NdeII GATC 5 cut(s) 81, 865, 879, 886, 1388
NlaIII CATG 3 cut(s) 527, 608, 972
NlaIV GGNNCC 3 cut(s) 319, 753, 1046
NmuCI GTSAC 4 cut(s) 61, 655, 923, 1004
NsbI TGCGCA 1 cut(s) 1241
NsiI ATGCAT 1 cut(s) 1027
PciSI GCTCTTC 1 cut(s) 1179
PctI GAATGC 3 cut(s) 280, 469, 553
PfeI GAWTC 4 cut(s) 518, 563, 770, 980
PflFI GACNNNGTC 2 cut(s) 116, 332
PfoI TCCNGGA 1 cut(s) 1223
PkrI GCNGC 5 cut(s) 139, 192, 317, 532, 1069
PleI GAGTC 1 cut(s) 662
PpsI GAGTC 1 cut(s) 662
PsiI TTATAA 1 cut(s) 572
Psp6I CCWGG 3 cut(s) 754, 850, 1047
PspFI CCCAGC 3 cut(s) 222, 310, 467
PspGI CCWGG 3 cut(s) 754, 850, 1047
PspN4I GGNNCC 3 cut(s) 319, 753, 1046
PspPI GGNCC 3 cut(s) 510, 848, 1051
PstNI CAGNNNCTG 2 cut(s) 725, 1041
PsuI RGATCY 3 cut(s) 865, 879, 1388
PsyI GACNNNGTC 2 cut(s) 116, 332
PvuII CAGCTG 1 cut(s) 137
RsaI GTAC 3 cut(s) 56, 822, 1046
RsaNI GTAC 3 cut(s) 55, 821, 1045
SapI GCTCTTC 1 cut(s) 1179
SaqAI TTAA 2 cut(s) 243, 942
SatI GCNGC 5 cut(s) 138, 191, 316, 531, 1068
Sau3AI GATC 5 cut(s) 81, 865, 879, 886, 1388
Sau96I GGNCC 3 cut(s) 510, 848, 1051
ScaI AGTACT 1 cut(s) 822
SchI GAGTC 1 cut(s) 663
ScrFI CCNGG 5 cut(s) 756, 852, 863, 1049, 1225
SduI GDGCHC 3 cut(s) 22, 104, 862
SfaNI GCATC 2 cut(s) 1079, 1192
SinI GGWCC 3 cut(s) 510, 848, 1051
SmlI CTYRAG 1 cut(s) 941
SmoI CTYRAG 1 cut(s) 941
SpeI ACTAGT 1 cut(s) 742
Sse9I AATT 6 cut(s) 600, 728, 946, 1117, 1281, 1362
SsiI CCGC 2 cut(s) 191, 315
SspMI CTAG 4 cut(s) 432, 617, 743, 1397
StyD4I CCNGG 5 cut(s) 754, 850, 861, 1047, 1223
StyI CCWWGG 3 cut(s) 253, 523, 844
TaaI ACNGT 6 cut(s) 118, 356, 396, 583, 1001, 1270
TaqI TCGA 2 cut(s) 80, 885
TasI AATT 6 cut(s) 600, 728, 946, 1117, 1281, 1362
TatI WGTACW 1 cut(s) 820
TauI GCSGC 2 cut(s) 193, 318
TfiI GAWTC 4 cut(s) 518, 563, 770, 980
Tru1I TTAA 2 cut(s) 243, 942
Tru9I TTAA 2 cut(s) 243, 942
TscAI CASTG 6 cut(s) 70, 121, 504, 928, 969, 1006
TseFI GTSAC 4 cut(s) 61, 655, 923, 1004
TseI GCWGC 3 cut(s) 137, 530, 1067
Tsp45I GTSAC 4 cut(s) 61, 655, 923, 1004
TspDTI ATGAA 5 cut(s) 363, 456, 593, 639, 1320
TspGWI ACGGA 1 cut(s) 119
TspRI CASTG 6 cut(s) 70, 121, 504, 928, 969, 1006
Tth111I GACNNNGTC 2 cut(s) 116, 332
Vha464I CTTAAG 1 cut(s) 941
VpaK11BI GGWCC 3 cut(s) 510, 848, 1051
XapI RAATTY 1 cut(s) 946
XmiI GTMKAC 1 cut(s) 249
XspI CTAG 4 cut(s) 432, 617, 743, 1397
ZrmI AGTACT 1 cut(s) 822
Zsp2I ATGCAT 1 cut(s) 1027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.