RchiOBHm_Chr4g0425011

FAD-dependent monooxygenase required for the C5-ring hydroxylation during ubiquinone biosynthesis. Catalyzes the hydroxylation of 3-polyprenyl-4-hydroxybenzoic acid to 3- polyprenyl-4,5-dihydroxybenzoic acid. The electrons required for the hydroxylation reaction may be funneled indirectly from NADPH via a ferredoxin ferredoxin reductase system to COQ6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0006427
Physical Location & Seq
Forward (+)
50667294 .. 50670329
3036 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39425

Sequence Viewer

Length: 648 bp
ATGGGTTTCGATCATGTGGCACAGCAGAAATCTTCAAATAAAAGTGAACCGTATGACATTGCTATTGTTGGAGGAGGCCTGGTTGGCATGGCCCTAGCTAGTTCCTTGGGTAGTTGGTCTTTTGTTTTGTTATCTGCAGATTTCTACAGTGACATACTAATATATGTCGATTCTGTGTACTTAGTGGACAATTGCCCCCATGATGACTCTTCCTCGGATGCTCTGTTGTGCTGCAGCTGTTGGTCTATGATATCACAAGCAAACAAGGGTACTAAATTGCTGTGTCCTAAATACGAACCCTACTTCTGGTCAAATGCAAACAAGGGTACTTTTCTATTCTCATTGCTGAGAATTGGTATTCAAGTTTATGAATTTCCTGCAAGGAAGGTAAATAATGGCTCCTGGGAATTTTGCAGCTTGAATCTTCTATTGCATATTCTGGATTTGCACTTGGTTATGTTTACGGTGGCTTGGCAAGTTCTGGAGGTGGTAGCTAGTTGTGACAGTGACATAGTTCAGCTGAGATTTTGTGTGTGTCTTCTTGGTGGACTAATGGCTCTGGGATACACAATTGCTACTATCCTTAAAAGAAGCAGTCATGTATTCTTCAATGATGGTTTGGACATGGGTTTACATGAGGAAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01100 ko01110 map00130 map01100 map01110
Pfam Domains
Protein Families
DUF1232 FAD_binding_3

Protein Analysis

215

Amino Acids

23.76

Weight (kDa)

4.92

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 371, 407
AfaI GTAC 3 cut(s) 179, 271, 328
AfiI CCNNNNNNNGG 1 cut(s) 306
AgsI TTSAA 4 cut(s) 36, 362, 421, 610
AjnI CCWGG 2 cut(s) 78, 401
AluBI AGCT 5 cut(s) 98, 237, 417, 494, 520
AluI AGCT 5 cut(s) 98, 237, 417, 494, 520
AoxI GGCC 2 cut(s) 76, 90
ApeKI GCWGC 3 cut(s) 231, 234, 414
ApoI RAATTY 2 cut(s) 371, 407
AspS9I GGNCC 1 cut(s) 91
BbsI GAAGAC 1 cut(s) 530
BbvI GCAGC 3 cut(s) 218, 246, 426
BccI CCATC 1 cut(s) 608
BciT130I CCWGG 2 cut(s) 80, 403
BciVI GTATCC 1 cut(s) 557
BfaI CTAG 3 cut(s) 95, 99, 495
BfmI CTRYAG 3 cut(s) 135, 145, 232
BfuI GTATCC 1 cut(s) 557
BglI GCCNNNNNGGC 1 cut(s) 84
BisI GCNGC 3 cut(s) 232, 235, 415
BlsI GCNGC 3 cut(s) 233, 236, 416
Bme1390I CCNGG 2 cut(s) 80, 403
BmgT120I GGNCC 1 cut(s) 91
BmiI GGNNCC 1 cut(s) 400
BmrFI CCNGG 2 cut(s) 80, 403
BmsI GCATC 1 cut(s) 208
BpiI GAAGAC 1 cut(s) 530
BpmI CTGGAG 1 cut(s) 503
BsaJI CCNNGG 3 cut(s) 105, 213, 402
BsaXI ACNNNNNCTCC 2 cut(s) 66, 96
Bsc4I CCNNNNNNNGG 1 cut(s) 306
Bse3DI GCAATG 2 cut(s) 57, 341
BseBI CCWGG 2 cut(s) 80, 403
BseDI CCNNGG 3 cut(s) 105, 213, 402
BseGI GGATG 1 cut(s) 223
BseLI CCNNNNNNNGG 1 cut(s) 306
BseMI GCAATG 2 cut(s) 57, 341
BseMII CTCAG 2 cut(s) 338, 512
BseRI GAGGAG 1 cut(s) 87
BseXI GCAGC 3 cut(s) 218, 246, 426
BshFI GGCC 2 cut(s) 78, 92
BslI CCNNNNNNNGG 1 cut(s) 306
BsnI GGCC 2 cut(s) 78, 92
Bsp143I GATC 1 cut(s) 10
BspANI GGCC 2 cut(s) 78, 92
BspCNI CTCAG 2 cut(s) 339, 513
BspLI GGNNCC 1 cut(s) 400
BspMAI CTGCAG 2 cut(s) 139, 236
BsrDI GCAATG 2 cut(s) 57, 341
BssECI CCNNGG 3 cut(s) 105, 213, 402
BssMI GATC 1 cut(s) 10
BssT1I CCWWGG 1 cut(s) 105
Bst2UI CCWGG 2 cut(s) 80, 403
Bst4CI ACNGT 4 cut(s) 51, 149, 466, 506
Bst6I CTCTTC 1 cut(s) 214
BstDEI CTNAG 3 cut(s) 181, 347, 521
BstF5I GGATG 1 cut(s) 223
BstKTI GATC 1 cut(s) 13
BstMBI GATC 1 cut(s) 10
BstMWI GCNNNNNNNGC 1 cut(s) 84
BstNI CCWGG 2 cut(s) 80, 403
BstSCI CCNGG 2 cut(s) 78, 401
BstSFI CTRYAG 3 cut(s) 135, 145, 232
BstV1I GCAGC 3 cut(s) 218, 246, 426
BstV2I GAAGAC 1 cut(s) 530
BsuI GTATCC 1 cut(s) 557
BsuRI GGCC 2 cut(s) 78, 92
BtsCI GGATG 1 cut(s) 223
BtsIMutI CAGTG 2 cut(s) 154, 511
Cfr13I GGNCC 1 cut(s) 91
Csp6I GTAC 3 cut(s) 178, 270, 327
CviAII CATG 6 cut(s) 14, 88, 200, 599, 625, 635
CviQI GTAC 3 cut(s) 178, 270, 327
DdeI CTNAG 3 cut(s) 181, 347, 521
DpnI GATC 1 cut(s) 12
DpnII GATC 1 cut(s) 10
Eam1104I CTCTTC 1 cut(s) 214
EarI CTCTTC 1 cut(s) 214
Eco130I CCWWGG 1 cut(s) 105
Eco147I AGGCCT 1 cut(s) 78
Eco32I GATATC 1 cut(s) 252
EcoRII CCWGG 2 cut(s) 78, 401
EcoRV GATATC 1 cut(s) 252
EcoT14I CCWWGG 1 cut(s) 105
ErhI CCWWGG 1 cut(s) 105
FaeI CATG 6 cut(s) 17, 91, 203, 602, 628, 638
FatI CATG 6 cut(s) 13, 87, 199, 598, 624, 634
Fnu4HI GCNGC 3 cut(s) 232, 235, 415
FokI GGATG 1 cut(s) 230
Fsp4HI GCNGC 3 cut(s) 232, 235, 415
FspBI CTAG 3 cut(s) 95, 99, 495
GluI GCNGC 3 cut(s) 232, 235, 415
GsuI CTGGAG 1 cut(s) 503
HaeIII GGCC 2 cut(s) 78, 92
Hin1II CATG 6 cut(s) 17, 91, 203, 602, 628, 638
HinfI GANTC 3 cut(s) 170, 206, 421
Hpy166II GTNNAC 6 cut(s) 47, 178, 187, 462, 548, 632
Hpy188I TCNGA 1 cut(s) 217
Hpy188III TCNNGA 2 cut(s) 440, 482
Hpy8I GTNNAC 6 cut(s) 47, 178, 187, 462, 548, 632
HpyAV CCTTC 2 cut(s) 379, 635
HpyCH4III ACNGT 4 cut(s) 51, 149, 466, 506
HpyCH4V TGCA 7 cut(s) 137, 234, 317, 380, 414, 433, 448
HpyF10VI GCNNNNNNNGC 1 cut(s) 84
HpyF3I CTNAG 3 cut(s) 181, 347, 521
Hsp92II CATG 6 cut(s) 17, 91, 203, 602, 628, 638
Kzo9I GATC 1 cut(s) 10
LmnI GCTCC 1 cut(s) 404
LpnPI CCDG 9 cut(s) 65, 92, 292, 388, 390, 415, 425, 467, 545
Lsp1109I GCAGC 3 cut(s) 218, 246, 426
LweI GCATC 1 cut(s) 208
MaeI CTAG 3 cut(s) 95, 99, 495
MaeIII GTNAC 3 cut(s) 149, 500, 506
MalI GATC 1 cut(s) 12
MboI GATC 1 cut(s) 10
MboII GAAGA 5 cut(s) 24, 201, 416, 530, 598
MfeI CAATTG 2 cut(s) 190, 570
MluCI AATT 6 cut(s) 190, 275, 351, 371, 407, 570
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 1 cut(s) 49
MnlI CCTC 5 cut(s) 65, 68, 223, 478, 631
MseI TTAA 1 cut(s) 585
MspA1I CMGCKG 2 cut(s) 237, 520
MspR9I CCNGG 2 cut(s) 80, 403
MunI CAATTG 2 cut(s) 190, 570
MvaI CCWGG 2 cut(s) 80, 403
MwoI GCNNNNNNNGC 1 cut(s) 84
NdeII GATC 1 cut(s) 10
NlaIII CATG 6 cut(s) 17, 91, 203, 602, 628, 638
NlaIV GGNNCC 1 cut(s) 400
NmuCI GTSAC 3 cut(s) 149, 500, 506
PceI AGGCCT 1 cut(s) 78
PfeI GAWTC 2 cut(s) 170, 421
PkrI GCNGC 3 cut(s) 233, 236, 416
PleI GAGTC 1 cut(s) 200
PpsI GAGTC 1 cut(s) 200
Psp6I CCWGG 2 cut(s) 78, 401
PspGI CCWGG 2 cut(s) 78, 401
PspN4I GGNNCC 1 cut(s) 400
PspPI GGNCC 1 cut(s) 91
PstI CTGCAG 2 cut(s) 139, 236
PvuII CAGCTG 2 cut(s) 237, 520
RsaI GTAC 3 cut(s) 179, 271, 328
RsaNI GTAC 3 cut(s) 178, 270, 327
SaqAI TTAA 1 cut(s) 585
SatI GCNGC 3 cut(s) 232, 235, 415
Sau3AI GATC 1 cut(s) 10
Sau96I GGNCC 1 cut(s) 91
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 2 cut(s) 80, 403
SetI ASST 7 cut(s) 100, 239, 390, 419, 489, 496, 522
SfaNI GCATC 1 cut(s) 208
SfcI CTRYAG 3 cut(s) 135, 145, 232
Sse9I AATT 6 cut(s) 190, 275, 351, 371, 407, 570
SseBI AGGCCT 1 cut(s) 78
SspMI CTAG 3 cut(s) 95, 99, 495
StuI AGGCCT 1 cut(s) 78
StyD4I CCNGG 2 cut(s) 78, 401
StyI CCWWGG 1 cut(s) 105
TaaI ACNGT 4 cut(s) 51, 149, 466, 506
TaqI TCGA 2 cut(s) 9, 168
TasI AATT 6 cut(s) 190, 275, 351, 371, 407, 570
TatI WGTACW 1 cut(s) 177
TfiI GAWTC 2 cut(s) 170, 421
Tru1I TTAA 1 cut(s) 585
Tru9I TTAA 1 cut(s) 585
TscAI CASTG 2 cut(s) 154, 511
TseFI GTSAC 3 cut(s) 149, 500, 506
TseI GCWGC 3 cut(s) 231, 234, 414
Tsp45I GTSAC 3 cut(s) 149, 500, 506
TspDTI ATGAA 1 cut(s) 384
TspRI CASTG 2 cut(s) 154, 511
XapI RAATTY 2 cut(s) 371, 407
XspI CTAG 3 cut(s) 95, 99, 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.