RLG00000023395

FAD-dependent monooxygenase required for the C5-ring hydroxylation during ubiquinone biosynthesis. Catalyzes the hydroxylation of 3-polyprenyl-4-hydroxybenzoic acid to 3- polyprenyl-4,5-dihydroxybenzoic acid. The electrons required for the hydroxylation reaction may be funneled indirectly from NADPH via a ferredoxin ferredoxin reductase system to COQ6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0006427
Physical Location & Seq
Forward (+)
23037153 .. 23039322
2170 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023395

Sequence Viewer

Length: 495 bp
ATGAAAGACAACAAAGGAGCCCAGTTCATTGGCCCAAATCCTAGCCCTAGGAGAGTAAGGCCCAATGCCAAAAAGCCTAGAACAACAACGTACCAAGCCATTACTGTCACCTGCATCGAGCGTCGCTGGACTTCCACCTTGCCTACTGCCCCAACCAAGAAAGTCGGAACCGGCAGCACCAGTCGGGCGGAACTAGCCATACCAGCAACAGGACCAGAGGACCTCTACCGTTGTCAACAGGGATATTGGCAATTGAAACTTTCACATAAGGGTTTTTGTACTGGTGCAGCTGCTAAAGTTTCCAGCTCCAATGATCATGTGGGGCAGCAGAAATCTTCAAATAAAAGTGAACCATATGACATTGCTATTGTTGAATGTGGCATGGTTGGCGTGGCCCTTGCTTGTTCCTTGGGTAGGCAGGTGAGATTTCGGCAAATCCTTTTCAAGCGTGCTCAAGAGGATGGAGAAGCCAGGCCAGAGACCTTAGCTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01100 ko01110 map00130 map01100 map01110
Pfam Domains
Protein Families
DUF1232 FAD_binding_3

Protein Analysis

165

Amino Acids

17.95

Weight (kDa)

9.84

Isoelectric Point (pI)

50.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 119, 409
Acc36I ACCTGC 2 cut(s) 119, 409
AciI CCGC 1 cut(s) 188
AfaI GTAC 2 cut(s) 92, 280
AfiI CCNNNNNNNGG 2 cut(s) 209, 414
AgsI TTSAA 4 cut(s) 256, 339, 374, 445
AjnI CCWGG 1 cut(s) 470
AluBI AGCT 3 cut(s) 290, 306, 488
AluI AGCT 3 cut(s) 290, 306, 488
Alw21I GWGCWC 1 cut(s) 454
Alw26I GTCTC 1 cut(s) 473
AoxI GGCC 4 cut(s) 31, 59, 393, 473
ApeKI GCWGC 4 cut(s) 174, 287, 290, 325
AspA2I CCTAGG 1 cut(s) 47
AspS9I GGNCC 5 cut(s) 32, 60, 212, 220, 394
AsuHPI GGTGA 2 cut(s) 100, 433
AvaII GGWCC 2 cut(s) 212, 220
AvrII CCTAGG 1 cut(s) 47
BanII GRGCYC 1 cut(s) 22
Bbv12I GWGCWC 1 cut(s) 454
BbvI GCAGC 4 cut(s) 186, 277, 299, 337
BccI CCATC 1 cut(s) 455
BciT130I CCWGG 1 cut(s) 472
BclI TGATCA 1 cut(s) 313
BcoDI GTCTC 1 cut(s) 473
BfaI CTAG 4 cut(s) 42, 48, 78, 194
BfuAI ACCTGC 2 cut(s) 119, 409
BisI GCNGC 4 cut(s) 175, 288, 291, 326
BlnI CCTAGG 1 cut(s) 47
BlsI GCNGC 4 cut(s) 176, 289, 292, 327
Bme1390I CCNGG 1 cut(s) 472
Bme18I GGWCC 2 cut(s) 212, 220
BmgT120I GGNCC 5 cut(s) 32, 60, 212, 220, 394
BmiI GGNNCC 2 cut(s) 19, 169
BmrFI CCNGG 1 cut(s) 472
BmrI ACTGGG 1 cut(s) 16
BmsI GCATC 1 cut(s) 123
BmuI ACTGGG 1 cut(s) 16
Bpu10I CCTNAGC 1 cut(s) 484
BpuEI CTTGAG 1 cut(s) 438
BsaI GGTCTC 1 cut(s) 473
BsaJI CCNNGG 2 cut(s) 47, 408
Bsc4I CCNNNNNNNGG 2 cut(s) 209, 414
Bse118I RCCGGY 1 cut(s) 170
Bse1I ACTGG 3 cut(s) 22, 180, 286
Bse3DI GCAATG 1 cut(s) 360
BseBI CCWGG 1 cut(s) 472
BseDI CCNNGG 2 cut(s) 47, 408
BseGI GGATG 1 cut(s) 466
BseLI CCNNNNNNNGG 2 cut(s) 209, 414
BseMI GCAATG 1 cut(s) 360
BseNI ACTGG 3 cut(s) 22, 180, 286
BseXI GCAGC 4 cut(s) 186, 277, 299, 337
BsgI GTGCAG 1 cut(s) 306
BshFI GGCC 4 cut(s) 33, 61, 395, 475
BsiHKAI GWGCWC 1 cut(s) 454
BsiSI CCGG 1 cut(s) 171
BslI CCNNNNNNNGG 2 cut(s) 209, 414
BsmAI GTCTC 1 cut(s) 473
BsnI GGCC 4 cut(s) 33, 61, 395, 475
Bso31I GGTCTC 1 cut(s) 473
Bsp1286I GDGCHC 2 cut(s) 22, 454
Bsp143I GATC 1 cut(s) 313
BspACI CCGC 1 cut(s) 188
BspANI GGCC 4 cut(s) 33, 61, 395, 475
BspLI GGNNCC 2 cut(s) 19, 169
BspMI ACCTGC 2 cut(s) 119, 409
BspTNI GGTCTC 1 cut(s) 473
BsrDI GCAATG 1 cut(s) 360
BsrFI RCCGGY 1 cut(s) 170
BsrI ACTGG 3 cut(s) 22, 180, 286
BssAI RCCGGY 1 cut(s) 170
BssECI CCNNGG 2 cut(s) 47, 408
BssMI GATC 1 cut(s) 313
BssT1I CCWWGG 2 cut(s) 47, 408
Bst2UI CCWGG 1 cut(s) 472
Bst4CI ACNGT 2 cut(s) 106, 230
BstC8I GCNNGC 1 cut(s) 450
BstDEI CTNAG 2 cut(s) 484, 489
BstENI CCTNNNNNAGG 1 cut(s) 412
BstF5I GGATG 1 cut(s) 466
BstKTI GATC 1 cut(s) 316
BstMAI GTCTC 1 cut(s) 473
BstMBI GATC 1 cut(s) 313
BstMWI GCNNNNNNNGC 3 cut(s) 194, 203, 387
BstNI CCWGG 1 cut(s) 472
BstSCI CCNGG 1 cut(s) 470
BstV1I GCAGC 4 cut(s) 186, 277, 299, 337
BstXI CCANNNNNNTGG 1 cut(s) 29
BsuRI GGCC 4 cut(s) 33, 61, 395, 475
BtsCI GGATG 1 cut(s) 466
BveI ACCTGC 2 cut(s) 119, 409
Cac8I GCNNGC 1 cut(s) 450
Cfr10I RCCGGY 1 cut(s) 170
Cfr13I GGNCC 5 cut(s) 32, 60, 212, 220, 394
CseI GACGC 1 cut(s) 110
Csp6I GTAC 2 cut(s) 91, 279
CviAII CATG 2 cut(s) 317, 382
CviQI GTAC 2 cut(s) 91, 279
DdeI CTNAG 2 cut(s) 484, 489
DpnI GATC 1 cut(s) 315
DpnII GATC 1 cut(s) 313
EciI GGCGGA 1 cut(s) 203
Eco130I CCWWGG 2 cut(s) 47, 408
Eco24I GRGCYC 1 cut(s) 22
Eco31I GGTCTC 1 cut(s) 473
Eco47I GGWCC 2 cut(s) 212, 220
EcoNI CCTNNNNNAGG 1 cut(s) 412
EcoO109I RGGNCCY 1 cut(s) 220
EcoRII CCWGG 1 cut(s) 470
EcoT14I CCWWGG 2 cut(s) 47, 408
EcoT38I GRGCYC 1 cut(s) 22
ErhI CCWWGG 2 cut(s) 47, 408
FaeI CATG 2 cut(s) 320, 385
FaiI YATR 6 cut(s) 200, 267, 318, 355, 357, 383
FatI CATG 2 cut(s) 316, 381
FauNDI CATATG 1 cut(s) 355
FbaI TGATCA 1 cut(s) 313
Fnu4HI GCNGC 4 cut(s) 175, 288, 291, 326
FokI GGATG 1 cut(s) 473
FriOI GRGCYC 1 cut(s) 22
Fsp4HI GCNGC 4 cut(s) 175, 288, 291, 326
FspBI CTAG 4 cut(s) 42, 48, 78, 194
GluI GCNGC 4 cut(s) 175, 288, 291, 326
HaeIII GGCC 4 cut(s) 33, 61, 395, 475
HapII CCGG 1 cut(s) 171
HgaI GACGC 1 cut(s) 110
Hin1II CATG 2 cut(s) 320, 385
HincII GTYRAC 1 cut(s) 236
HindII GTYRAC 1 cut(s) 236
HpaII CCGG 1 cut(s) 171
HphI GGTGA 2 cut(s) 100, 433
Hpy166II GTNNAC 2 cut(s) 236, 350
Hpy188I TCNGA 1 cut(s) 167
Hpy188III TCNNGA 1 cut(s) 455
Hpy8I GTNNAC 2 cut(s) 236, 350
Hpy99I CGWCG 1 cut(s) 126
HpyCH4III ACNGT 2 cut(s) 106, 230
HpyCH4IV ACGT 1 cut(s) 89
HpyCH4V TGCA 2 cut(s) 114, 287
HpyF10VI GCNNNNNNNGC 3 cut(s) 194, 203, 387
HpyF3I CTNAG 2 cut(s) 484, 489
HpySE526I ACGT 1 cut(s) 89
Hsp92II CATG 2 cut(s) 320, 385
Ksp22I TGATCA 1 cut(s) 313
Kzo9I GATC 1 cut(s) 313
LmnI GCTCC 2 cut(s) 17, 311
Lsp1109I GCAGC 4 cut(s) 186, 277, 299, 337
LweI GCATC 1 cut(s) 123
MaeI CTAG 4 cut(s) 42, 48, 78, 194
MaeII ACGT 1 cut(s) 89
MaeIII GTNAC 1 cut(s) 106
MalI GATC 1 cut(s) 315
MboI GATC 1 cut(s) 313
MboII GAAGA 1 cut(s) 327
MfeI CAATTG 1 cut(s) 251
MhlI GDGCHC 2 cut(s) 22, 454
MluCI AATT 1 cut(s) 251
MmeI TCCRAC 1 cut(s) 145
MnlI CCTC 3 cut(s) 211, 233, 451
MspA1I CMGCKG 1 cut(s) 290
MspI CCGG 1 cut(s) 171
MspR9I CCNGG 1 cut(s) 472
MunI CAATTG 1 cut(s) 251
MvaI CCWGG 1 cut(s) 472
MwoI GCNNNNNNNGC 3 cut(s) 194, 203, 387
NdeI CATATG 1 cut(s) 355
NdeII GATC 1 cut(s) 313
NlaIII CATG 2 cut(s) 320, 385
NlaIV GGNNCC 2 cut(s) 19, 169
NmuCI GTSAC 1 cut(s) 106
PaqCI CACCTGC 2 cut(s) 119, 409
PkrI GCNGC 4 cut(s) 176, 289, 292, 327
PpuMI RGGWCCY 1 cut(s) 220
Psp5II RGGWCCY 1 cut(s) 220
Psp6I CCWGG 1 cut(s) 470
PspGI CCWGG 1 cut(s) 470
PspN4I GGNNCC 2 cut(s) 19, 169
PspPI GGNCC 5 cut(s) 32, 60, 212, 220, 394
PspPPI RGGWCCY 1 cut(s) 220
PsrI GAACNNNNNNTAC 2 cut(s) 183, 215
PvuII CAGCTG 1 cut(s) 290
RsaI GTAC 2 cut(s) 92, 280
RsaNI GTAC 2 cut(s) 91, 279
SatI GCNGC 4 cut(s) 175, 288, 291, 326
Sau3AI GATC 1 cut(s) 313
Sau96I GGNCC 5 cut(s) 32, 60, 212, 220, 394
ScrFI CCNGG 1 cut(s) 472
SduI GDGCHC 2 cut(s) 22, 454
SetI ASST 9 cut(s) 92, 113, 140, 225, 292, 308, 423, 485, 490
SfaNI GCATC 1 cut(s) 123
SinI GGWCC 2 cut(s) 212, 220
SmlI CTYRAG 1 cut(s) 453
SmoI CTYRAG 1 cut(s) 453
Sse9I AATT 1 cut(s) 251
SsiI CCGC 1 cut(s) 188
SspMI CTAG 4 cut(s) 42, 48, 78, 194
StyD4I CCNGG 1 cut(s) 470
StyI CCWWGG 2 cut(s) 47, 408
TaaI ACNGT 2 cut(s) 106, 230
TaiI ACGT 1 cut(s) 92
TaqI TCGA 1 cut(s) 117
TasI AATT 1 cut(s) 251
TatI WGTACW 1 cut(s) 278
TseFI GTSAC 1 cut(s) 106
TseI GCWGC 4 cut(s) 174, 287, 290, 325
Tsp45I GTSAC 1 cut(s) 106
TspDTI ATGAA 2 cut(s) 16, 17
VpaK11BI GGWCC 2 cut(s) 212, 220
XagI CCTNNNNNAGG 1 cut(s) 412
XcmI CCANNNNNNNNNTGG 1 cut(s) 316
XmaJI CCTAGG 1 cut(s) 47
XspI CTAG 4 cut(s) 42, 48, 78, 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.