FAD-dependent monooxygenase required for the C5-ring hydroxylation during ubiquinone biosynthesis. Catalyzes the hydroxylation of 3-polyprenyl-4-hydroxybenzoic acid to 3- polyprenyl-4,5-dihydroxybenzoic acid. The electrons required for the hydroxylation reaction may be funneled indirectly from NADPH via a ferredoxin ferredoxin reductase system to COQ6
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| Species | Orthologous Gene IDs |
|---|---|
| rosa_chinensis | RchiOBHm_Chr4g0414911 RchiOBHm_Chr4g0425011 |
| rosa_laevigata | RLG00000001467 RLG00000023395 RLG00000031447 RLG00000034760 |
| rosa_multiflora | Rmu_sc0000321.1_g000004 Rmu_sc0003921.1_g000012 Rmu_sc0032413.1_g000001 Rmu_sc0036978.1_g000001 |
| rosa_roxburghii | Rroxscaffold_2G00151150 Rroxscaffold_3G00257780 Rroxscaffold_4G00282760 Rroxscaffold_4G00295240 Rroxscaffold_4G00316850 Rroxscaffold_5G00343780 |
| rosa_samantha | Rh1AG079100 Rh2BG126800 Rh3CG293900 Rh3DG288300 Rh5AG378800 Rh5BG350000 Rh6AG284800 Rh6BG298800 Rh6CG115900 Rh7DG284600 |
| rosa_wichuraiana | Rw5G032050 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AfaI | GTAC | 1 cut(s) | 70 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 204 |
| AgsI | TTSAA | 2 cut(s) | 46, 129 |
| AluBI | AGCT | 2 cut(s) | 80, 96 |
| AluI | AGCT | 2 cut(s) | 80, 96 |
| AoxI | GGCC | 1 cut(s) | 183 |
| ApeKI | GCWGC | 3 cut(s) | 77, 80, 115 |
| AspS9I | GGNCC | 1 cut(s) | 184 |
| BbvI | GCAGC | 3 cut(s) | 67, 89, 127 |
| BclI | TGATCA | 1 cut(s) | 103 |
| BfaI | CTAG | 1 cut(s) | 32 |
| BisI | GCNGC | 3 cut(s) | 78, 81, 116 |
| BlsI | GCNGC | 3 cut(s) | 79, 82, 117 |
| BmgT120I | GGNCC | 1 cut(s) | 184 |
| BmsI | GCATC | 1 cut(s) | 29 |
| BsaJI | CCNNGG | 1 cut(s) | 198 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 204 |
| Bse1I | ACTGG | 1 cut(s) | 76 |
| Bse3DI | GCAATG | 1 cut(s) | 150 |
| BseDI | CCNNGG | 1 cut(s) | 198 |
| BseGI | GGATG | 1 cut(s) | 170 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 204 |
| BseMI | GCAATG | 1 cut(s) | 150 |
| BseNI | ACTGG | 1 cut(s) | 76 |
| BseXI | GCAGC | 3 cut(s) | 67, 89, 127 |
| BsgI | GTGCAG | 1 cut(s) | 96 |
| BshFI | GGCC | 1 cut(s) | 185 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 204 |
| BsnI | GGCC | 1 cut(s) | 185 |
| Bsp143I | GATC | 1 cut(s) | 103 |
| BspANI | GGCC | 1 cut(s) | 185 |
| BsrDI | GCAATG | 1 cut(s) | 150 |
| BsrI | ACTGG | 1 cut(s) | 76 |
| BssECI | CCNNGG | 1 cut(s) | 198 |
| BssMI | GATC | 1 cut(s) | 103 |
| BssT1I | CCWWGG | 1 cut(s) | 198 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 202 |
| BstF5I | GGATG | 1 cut(s) | 170 |
| BstKTI | GATC | 1 cut(s) | 106 |
| BstMBI | GATC | 1 cut(s) | 103 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 177 |
| BstV1I | GCAGC | 3 cut(s) | 67, 89, 127 |
| BsuRI | GGCC | 1 cut(s) | 185 |
| BtsCI | GGATG | 1 cut(s) | 170 |
| Cfr13I | GGNCC | 1 cut(s) | 184 |
| Csp6I | GTAC | 1 cut(s) | 69 |
| CviAII | CATG | 2 cut(s) | 107, 172 |
| CviJI | RGCY | 3 cut(s) | 80, 96, 185 |
| CviKI_1 | RGCY | 3 cut(s) | 80, 96, 185 |
| CviQI | GTAC | 1 cut(s) | 69 |
| DpnI | GATC | 1 cut(s) | 105 |
| DpnII | GATC | 1 cut(s) | 103 |
| Eco130I | CCWWGG | 1 cut(s) | 198 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 202 |
| EcoT14I | CCWWGG | 1 cut(s) | 198 |
| ErhI | CCWWGG | 1 cut(s) | 198 |
| FaeI | CATG | 2 cut(s) | 110, 175 |
| FaiI | YATR | 6 cut(s) | 11, 57, 108, 120, 147, 173 |
| FatI | CATG | 2 cut(s) | 106, 171 |
| FbaI | TGATCA | 1 cut(s) | 103 |
| Fnu4HI | GCNGC | 3 cut(s) | 78, 81, 116 |
| FokI | GGATG | 1 cut(s) | 177 |
| Fsp4HI | GCNGC | 3 cut(s) | 78, 81, 116 |
| FspBI | CTAG | 1 cut(s) | 32 |
| GluI | GCNGC | 3 cut(s) | 78, 81, 116 |
| HaeIII | GGCC | 1 cut(s) | 185 |
| Hin1II | CATG | 2 cut(s) | 110, 175 |
| HinfI | GANTC | 1 cut(s) | 140 |
| Hpy188I | TCNGA | 1 cut(s) | 41 |
| Hpy188III | TCNNGA | 1 cut(s) | 32 |
| HpyCH4V | TGCA | 2 cut(s) | 20, 77 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 177 |
| Hsp92II | CATG | 2 cut(s) | 110, 175 |
| Ksp22I | TGATCA | 1 cut(s) | 103 |
| Kzo9I | GATC | 1 cut(s) | 103 |
| LmnI | GCTCC | 1 cut(s) | 101 |
| LpnPI | CCDG | 2 cut(s) | 57, 106 |
| Lsp1109I | GCAGC | 3 cut(s) | 67, 89, 127 |
| LweI | GCATC | 1 cut(s) | 29 |
| MaeI | CTAG | 1 cut(s) | 32 |
| MalI | GATC | 1 cut(s) | 105 |
| MboI | GATC | 1 cut(s) | 103 |
| MboII | GAAGA | 1 cut(s) | 117 |
| MmeI | TCCRAC | 1 cut(s) | 142 |
| MspA1I | CMGCKG | 1 cut(s) | 80 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 177 |
| NdeII | GATC | 1 cut(s) | 103 |
| NlaIII | CATG | 2 cut(s) | 110, 175 |
| PfeI | GAWTC | 1 cut(s) | 140 |
| PkrI | GCNGC | 3 cut(s) | 79, 82, 117 |
| PspPI | GGNCC | 1 cut(s) | 184 |
| PvuII | CAGCTG | 1 cut(s) | 80 |
| RsaI | GTAC | 1 cut(s) | 70 |
| RsaNI | GTAC | 1 cut(s) | 69 |
| SatI | GCNGC | 3 cut(s) | 78, 81, 116 |
| Sau3AI | GATC | 1 cut(s) | 103 |
| Sau96I | GGNCC | 1 cut(s) | 184 |
| SetI | ASST | 3 cut(s) | 82, 98, 209 |
| SfaNI | GCATC | 1 cut(s) | 29 |
| SspMI | CTAG | 1 cut(s) | 32 |
| StyI | CCWWGG | 1 cut(s) | 198 |
| TatI | WGTACW | 1 cut(s) | 68 |
| TfiI | GAWTC | 1 cut(s) | 140 |
| TseI | GCWGC | 3 cut(s) | 77, 80, 115 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 202 |
| XbaI | TCTAGA | 1 cut(s) | 31 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 106 |
| XspI | CTAG | 1 cut(s) | 32 |