Rroxscaffold_4G00295240

FAD-dependent monooxygenase required for the C5-ring hydroxylation during ubiquinone biosynthesis. Catalyzes the hydroxylation of 3-polyprenyl-4-hydroxybenzoic acid to 3- polyprenyl-4,5-dihydroxybenzoic acid. The electrons required for the hydroxylation reaction may be funneled indirectly from NADPH via a ferredoxin ferredoxin reductase system to COQ6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0006427
Physical Location & Seq
Reverse (-)
15231996 .. 15238787
6792 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00295240.1

Sequence Viewer

Length: 363 bp
ATGGCAGGCAAAAACGTTGAAATCCATGCTGATGTGAAGAATATTCTCATTGAGATGGTGGACAAGATCGTGAACATTTGCCCACTCAGTCCTAATCTCGTAGCTATGTGTAAAGCTCGCGTCTCCGTCGACATCAACATCCTCCGCCGCAAGGATTTCCTGGACTACGAGTCCTTCATCATCCAATGGGGAATGTCGATGACGAAGCACTTGAAGGTGGCCATCATTGATAGCAATCCCGTATTATCTAATGGACTCTCAATCAAGAAAGAGGACCCCCCGATGCAAGGTACCACTGCCTTAAGCAGCCCGATCACCATCCCGTTCCTCCTAACCTGCAGGATTATCCGCTACACCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01100 ko01110 map00130 map01100 map01110
Pfam Domains
Protein Families
DUF1232 FAD_binding_3

Protein Analysis

120

Amino Acids

13.44

Weight (kDa)

8.86

Isoelectric Point (pI)

45.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 344
Acc65I GGTACC 1 cut(s) 290
AccB1I GGYRCC 1 cut(s) 290
AccI GTMKAC 1 cut(s) 129
AccII CGCG 1 cut(s) 120
AciI CCGC 3 cut(s) 145, 148, 349
AclI AACGTT 1 cut(s) 15
AcoI YGGCCR 1 cut(s) 219
AfaI GTAC 1 cut(s) 292
AfiI CCNNNNNNNGG 2 cut(s) 151, 287
AflII CTTAAG 1 cut(s) 301
AgsI TTSAA 2 cut(s) 20, 214
AhdI GACNNNNNGTC 1 cut(s) 169
AjnI CCWGG 1 cut(s) 159
AluBI AGCT 2 cut(s) 104, 116
AluI AGCT 2 cut(s) 104, 116
Alw26I GTCTC 1 cut(s) 127
AoxI GGCC 1 cut(s) 219
ApeKI GCWGC 1 cut(s) 306
Asp718I GGTACC 1 cut(s) 290
AspS9I GGNCC 1 cut(s) 274
AsuHPI GGTGA 1 cut(s) 307
AvaII GGWCC 1 cut(s) 274
BalI TGGCCA 1 cut(s) 221
BanI GGYRCC 1 cut(s) 290
BbvI GCAGC 1 cut(s) 318
BccI CCATC 3 cut(s) 49, 230, 326
BciT130I CCWGG 1 cut(s) 161
BcoDI GTCTC 1 cut(s) 127
BfmI CTRYAG 1 cut(s) 337
BfrI CTTAAG 1 cut(s) 301
BfuAI ACCTGC 1 cut(s) 344
BisI GCNGC 2 cut(s) 148, 307
BlsI GCNGC 2 cut(s) 149, 308
Bme1390I CCNGG 1 cut(s) 161
Bme18I GGWCC 1 cut(s) 274
BmeRI GACNNNNNGTC 1 cut(s) 169
BmgT120I GGNCC 1 cut(s) 274
BmiI GGNNCC 2 cut(s) 276, 292
BmrFI CCNGG 1 cut(s) 161
BmsI GCATC 1 cut(s) 273
BsaBI GATNNNNATC 2 cut(s) 234, 317
Bsc4I CCNNNNNNNGG 2 cut(s) 151, 287
Bse8I GATNNNNATC 2 cut(s) 234, 317
BseBI CCWGG 1 cut(s) 161
BseGI GGATG 3 cut(s) 138, 180, 318
BseJI GATNNNNATC 2 cut(s) 234, 317
BseLI CCNNNNNNNGG 2 cut(s) 151, 287
BseMII CTCAG 1 cut(s) 100
BseXI GCAGC 1 cut(s) 318
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 1 cut(s) 221
BshNI GGYRCC 1 cut(s) 290
BslI CCNNNNNNNGG 2 cut(s) 151, 287
BsmAI GTCTC 1 cut(s) 127
BsmBI CGTCTC 1 cut(s) 127
BsnI GGCC 1 cut(s) 221
Bsp143I GATC 2 cut(s) 66, 312
BspACI CCGC 3 cut(s) 145, 148, 349
BspANI GGCC 1 cut(s) 221
BspCNI CTCAG 1 cut(s) 99
BspFNI CGCG 1 cut(s) 120
BspLI GGNNCC 2 cut(s) 276, 292
BspMAI CTGCAG 1 cut(s) 341
BspMI ACCTGC 1 cut(s) 344
BspT107I GGYRCC 1 cut(s) 290
BspTI CTTAAG 1 cut(s) 301
BssMI GATC 2 cut(s) 66, 312
Bst2UI CCWGG 1 cut(s) 161
BstAFI CTTAAG 1 cut(s) 301
BstC8I GCNNGC 2 cut(s) 7, 118
BstDEI CTNAG 1 cut(s) 86
BstF5I GGATG 3 cut(s) 138, 180, 318
BstFNI CGCG 1 cut(s) 120
BstKTI GATC 2 cut(s) 69, 315
BstMAI GTCTC 1 cut(s) 127
BstMBI GATC 2 cut(s) 66, 312
BstNI CCWGG 1 cut(s) 161
BstSCI CCNGG 1 cut(s) 159
BstSFI CTRYAG 1 cut(s) 337
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 1 cut(s) 318
BsuRI GGCC 1 cut(s) 221
BtsCI GGATG 3 cut(s) 138, 180, 318
BtsI GCAGTG 1 cut(s) 294
BtsIMutI CAGTG 1 cut(s) 294
BveI ACCTGC 1 cut(s) 344
Cac8I GCNNGC 2 cut(s) 7, 118
Cfr13I GGNCC 1 cut(s) 274
CseI GACGC 1 cut(s) 109
Csp6I GTAC 1 cut(s) 291
CviAII CATG 1 cut(s) 26
CviJI RGCY 4 cut(s) 104, 116, 221, 309
CviKI_1 RGCY 4 cut(s) 104, 116, 221, 309
CviQI GTAC 1 cut(s) 291
DdeI CTNAG 1 cut(s) 86
DpnI GATC 2 cut(s) 68, 314
DpnII GATC 2 cut(s) 66, 312
DriI GACNNNNNGTC 1 cut(s) 169
EaeI YGGCCR 1 cut(s) 219
Eam1105I GACNNNNNGTC 1 cut(s) 169
EciI GGCGGA 1 cut(s) 134
Eco47I GGWCC 1 cut(s) 274
EcoO109I RGGNCCY 1 cut(s) 274
EcoRII CCWGG 1 cut(s) 159
Esp3I CGTCTC 1 cut(s) 127
FaeI CATG 1 cut(s) 29
FaiI YATR 2 cut(s) 27, 107
FatI CATG 1 cut(s) 25
FblI GTMKAC 1 cut(s) 129
Fnu4HI GCNGC 2 cut(s) 148, 307
FokI GGATG 3 cut(s) 125, 167, 305
Fsp4HI GCNGC 2 cut(s) 148, 307
GluI GCNGC 2 cut(s) 148, 307
HaeIII GGCC 1 cut(s) 221
HgaI GACGC 1 cut(s) 109
Hin1II CATG 1 cut(s) 29
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HinfI GANTC 2 cut(s) 170, 255
HphI GGTGA 1 cut(s) 307
Hpy166II GTNNAC 3 cut(s) 61, 73, 130
Hpy188III TCNNGA 2 cut(s) 70, 265
Hpy8I GTNNAC 3 cut(s) 61, 73, 130
Hpy99I CGWCG 1 cut(s) 131
HpyAV CCTTC 2 cut(s) 184, 208
HpyCH4IV ACGT 1 cut(s) 15
HpyCH4V TGCA 2 cut(s) 286, 339
HpyF3I CTNAG 1 cut(s) 86
HpySE526I ACGT 1 cut(s) 15
Hsp92II CATG 1 cut(s) 29
KpnI GGTACC 1 cut(s) 294
Kzo9I GATC 2 cut(s) 66, 312
LpnPI CCDG 4 cut(s) 146, 173, 325, 349
Lsp1109I GCAGC 1 cut(s) 318
LweI GCATC 1 cut(s) 273
MaeII ACGT 1 cut(s) 15
MalI GATC 2 cut(s) 68, 314
MboI GATC 2 cut(s) 66, 312
MboII GAAGA 1 cut(s) 49
MlsI TGGCCA 1 cut(s) 221
MluNI TGGCCA 1 cut(s) 221
MlyI GAGTC 2 cut(s) 179, 249
MnlI CCTC 3 cut(s) 152, 265, 338
Mox20I TGGCCA 1 cut(s) 221
MscI TGGCCA 1 cut(s) 221
MseI TTAA 1 cut(s) 302
MslI CAYNNNNRTG 2 cut(s) 30, 53
Msp20I TGGCCA 1 cut(s) 221
MspCI CTTAAG 1 cut(s) 301
MspR9I CCNGG 1 cut(s) 161
MvaI CCWGG 1 cut(s) 161
MvnI CGCG 1 cut(s) 120
NdeII GATC 2 cut(s) 66, 312
NlaIII CATG 1 cut(s) 29
NlaIV GGNNCC 2 cut(s) 276, 292
PfoI TCCNGGA 1 cut(s) 159
PkrI GCNGC 2 cut(s) 149, 308
PleI GAGTC 2 cut(s) 178, 249
PpsI GAGTC 2 cut(s) 178, 249
PpuMI RGGWCCY 1 cut(s) 274
Psp1406I AACGTT 1 cut(s) 15
Psp5II RGGWCCY 1 cut(s) 274
Psp6I CCWGG 1 cut(s) 159
PspGI CCWGG 1 cut(s) 159
PspN4I GGNNCC 2 cut(s) 276, 292
PspPI GGNCC 1 cut(s) 274
PspPPI RGGWCCY 1 cut(s) 274
PstI CTGCAG 1 cut(s) 341
RsaI GTAC 1 cut(s) 292
RsaNI GTAC 1 cut(s) 291
RseI CAYNNNNRTG 2 cut(s) 30, 53
SalI GTCGAC 1 cut(s) 128
SaqAI TTAA 1 cut(s) 302
SatI GCNGC 2 cut(s) 148, 307
Sau3AI GATC 2 cut(s) 66, 312
Sau96I GGNCC 1 cut(s) 274
SbfI CCTGCAGG 1 cut(s) 341
SchI GAGTC 2 cut(s) 179, 249
ScrFI CCNGG 1 cut(s) 161
SdaI CCTGCAGG 1 cut(s) 341
SetI ASST 6 cut(s) 18, 106, 118, 219, 292, 338
SfaNI GCATC 1 cut(s) 273
SfcI CTRYAG 1 cut(s) 337
SinI GGWCC 1 cut(s) 274
SmiMI CAYNNNNRTG 2 cut(s) 30, 53
SmlI CTYRAG 1 cut(s) 301
SmoI CTYRAG 1 cut(s) 301
Sse8387I CCTGCAGG 1 cut(s) 341
SsiI CCGC 3 cut(s) 145, 148, 349
SspI AATATT 1 cut(s) 43
StyD4I CCNGG 1 cut(s) 159
TaiI ACGT 1 cut(s) 18
TaqI TCGA 2 cut(s) 129, 197
TauI GCSGC 1 cut(s) 150
Tru1I TTAA 1 cut(s) 302
Tru9I TTAA 1 cut(s) 302
TscAI CASTG 1 cut(s) 301
TseI GCWGC 1 cut(s) 306
TspDTI ATGAA 1 cut(s) 166
TspGWI ACGGA 1 cut(s) 115
TspRI CASTG 1 cut(s) 301
Vha464I CTTAAG 1 cut(s) 301
VpaK11BI GGWCC 1 cut(s) 274
XmiI GTMKAC 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.