Rroxscaffold_5G00343780

FAD-dependent monooxygenase required for the C5-ring hydroxylation during ubiquinone biosynthesis. Catalyzes the hydroxylation of 3-polyprenyl-4-hydroxybenzoic acid to 3- polyprenyl-4,5-dihydroxybenzoic acid. The electrons required for the hydroxylation reaction may be funneled indirectly from NADPH via a ferredoxin ferredoxin reductase system to COQ6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0006427
Physical Location & Seq
Reverse (-)
13465519 .. 13468788
3270 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00343780.1

Sequence Viewer

Length: 393 bp
ATGCCGGATCAAGAACCGTCGTCGCTATACAAAAGCACCTCAACCATGCTCGTCACCGCACACCTTCCTCTCTTTCGGACTCCGTTTTCCGATGTGGGGACGATGGTTCACTACCACCACGAGCACTCTAGTGCAGCTGCTAAAGTCTCCAGCTCCAATGGTGCTCAACCAAGCAATGATCATGTGGCACAGCAGAAATGTTCAAATAAAAGTGAACCGTATGACATTGCTATTGTTGGAGGAGGCATGGTTGGCATGACCCTAGCTAGTTCCTTGGCAAGAATGCCGATGACGAAGCACTTGAAGGTGGCCATCATTGATAGCAATCCTATATTATCTAATAGACTCTCAATCAAGAAAGAGGACCCCCCCCGATGCACGGGTTCAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01100 ko01110 map00130 map01100 map01110
Pfam Domains
Protein Families
DUF1232 FAD_binding_3

Protein Analysis

130

Amino Acids

13.96

Weight (kDa)

8.62

Isoelectric Point (pI)

44.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 57
AclWI GGATC 1 cut(s) 15
AcoI YGGCCR 1 cut(s) 309
AfiI CCNNNNNNNGG 2 cut(s) 96, 379
AgsI TTSAA 2 cut(s) 204, 304
AleI CACNNNNGTG 1 cut(s) 129
AluBI AGCT 3 cut(s) 137, 153, 266
AluI AGCT 3 cut(s) 137, 153, 266
Alw21I GWGCWC 2 cut(s) 126, 166
Alw26I GTCTC 1 cut(s) 151
AlwI GGATC 1 cut(s) 15
AoxI GGCC 1 cut(s) 309
ApeKI GCWGC 2 cut(s) 134, 137
AspS9I GGNCC 1 cut(s) 364
AsuHPI GGTGA 1 cut(s) 46
AvaII GGWCC 1 cut(s) 364
BalI TGGCCA 1 cut(s) 311
BauI CACGAG 1 cut(s) 119
Bbv12I GWGCWC 2 cut(s) 126, 166
BbvI GCAGC 2 cut(s) 124, 146
BccI CCATC 2 cut(s) 97, 320
BclI TGATCA 1 cut(s) 178
BcoDI GTCTC 1 cut(s) 151
BfaI CTAG 3 cut(s) 129, 263, 267
BisI GCNGC 2 cut(s) 135, 138
BlsI GCNGC 2 cut(s) 136, 139
Bme18I GGWCC 1 cut(s) 364
BmgT120I GGNCC 1 cut(s) 364
BmiI GGNNCC 1 cut(s) 366
BmsI GCATC 1 cut(s) 365
BpmI CTGGAG 1 cut(s) 133
BsaBI GATNNNNATC 1 cut(s) 324
BsaJI CCNNGG 1 cut(s) 273
BsaXI ACNNNNNCTCC 2 cut(s) 234, 264
Bsc4I CCNNNNNNNGG 2 cut(s) 96, 379
Bse3DI GCAATG 2 cut(s) 181, 225
Bse8I GATNNNNATC 1 cut(s) 324
BseDI CCNNGG 1 cut(s) 273
BseJI GATNNNNATC 1 cut(s) 324
BseLI CCNNNNNNNGG 2 cut(s) 96, 379
BseMI GCAATG 2 cut(s) 181, 225
BseRI GAGGAG 1 cut(s) 255
BseXI GCAGC 2 cut(s) 124, 146
BsgI GTGCAG 1 cut(s) 153
BshFI GGCC 1 cut(s) 311
BsiHKAI GWGCWC 2 cut(s) 126, 166
BsiSI CCGG 1 cut(s) 5
BslFI GGGAC 1 cut(s) 112
BslI CCNNNNNNNGG 2 cut(s) 96, 379
BsmAI GTCTC 1 cut(s) 151
BsmFI GGGAC 1 cut(s) 112
BsmI GAATGC 1 cut(s) 288
BsnI GGCC 1 cut(s) 311
Bsp1286I GDGCHC 2 cut(s) 126, 166
Bsp143I GATC 2 cut(s) 7, 178
BspACI CCGC 1 cut(s) 57
BspANI GGCC 1 cut(s) 311
BspLI GGNNCC 1 cut(s) 366
BspPI GGATC 1 cut(s) 15
BsrDI GCAATG 2 cut(s) 181, 225
BssECI CCNNGG 1 cut(s) 273
BssMI GATC 2 cut(s) 7, 178
BssSI CACGAG 1 cut(s) 119
BssT1I CCWWGG 1 cut(s) 273
Bst2BI CACGAG 1 cut(s) 119
Bst4CI ACNGT 2 cut(s) 18, 219
BstKTI GATC 2 cut(s) 10, 181
BstMAI GTCTC 1 cut(s) 151
BstMBI GATC 2 cut(s) 7, 178
BstMWI GCNNNNNNNGC 1 cut(s) 252
BstV1I GCAGC 2 cut(s) 124, 146
BsuRI GGCC 1 cut(s) 311
Cfr13I GGNCC 1 cut(s) 364
CviAII CATG 4 cut(s) 46, 182, 247, 256
CviJI RGCY 4 cut(s) 137, 153, 266, 311
CviKI_1 RGCY 4 cut(s) 137, 153, 266, 311
DpnI GATC 2 cut(s) 9, 180
DpnII GATC 2 cut(s) 7, 178
EaeI YGGCCR 1 cut(s) 309
Eco130I CCWWGG 1 cut(s) 273
Eco47I GGWCC 1 cut(s) 364
EcoO109I RGGNCCY 1 cut(s) 364
EcoT14I CCWWGG 1 cut(s) 273
ErhI CCWWGG 1 cut(s) 273
FaeI CATG 4 cut(s) 49, 185, 250, 259
FaiI YATR 8 cut(s) 28, 47, 183, 222, 248, 257, 332, 391
FaqI GGGAC 1 cut(s) 112
FatI CATG 4 cut(s) 45, 181, 246, 255
FbaI TGATCA 1 cut(s) 178
Fnu4HI GCNGC 2 cut(s) 135, 138
Fsp4HI GCNGC 2 cut(s) 135, 138
FspBI CTAG 3 cut(s) 129, 263, 267
GluI GCNGC 2 cut(s) 135, 138
GsuI CTGGAG 1 cut(s) 133
HaeIII GGCC 1 cut(s) 311
HapII CCGG 1 cut(s) 5
Hin1II CATG 4 cut(s) 49, 185, 250, 259
HinfI GANTC 2 cut(s) 79, 345
HpaII CCGG 1 cut(s) 5
HphI GGTGA 1 cut(s) 46
Hpy166II GTNNAC 2 cut(s) 109, 215
Hpy188I TCNGA 2 cut(s) 78, 91
Hpy188III TCNNGA 2 cut(s) 11, 355
Hpy8I GTNNAC 2 cut(s) 109, 215
Hpy99I CGWCG 2 cut(s) 22, 25
HpyAV CCTTC 2 cut(s) 74, 298
HpyCH4III ACNGT 2 cut(s) 18, 219
HpyCH4V TGCA 2 cut(s) 134, 378
HpyF10VI GCNNNNNNNGC 1 cut(s) 252
Hsp92II CATG 4 cut(s) 49, 185, 250, 259
Ksp22I TGATCA 1 cut(s) 178
Kzo9I GATC 2 cut(s) 7, 178
LmnI GCTCC 1 cut(s) 158
LpnPI CCDG 2 cut(s) 18, 163
Lsp1109I GCAGC 2 cut(s) 124, 146
LweI GCATC 1 cut(s) 365
MaeI CTAG 3 cut(s) 129, 263, 267
MaeIII GTNAC 1 cut(s) 52
MalI GATC 2 cut(s) 9, 180
MboI GATC 2 cut(s) 7, 178
MhlI GDGCHC 2 cut(s) 126, 166
MlsI TGGCCA 1 cut(s) 311
MluNI TGGCCA 1 cut(s) 311
MlyI GAGTC 2 cut(s) 73, 339
MmeI TCCRAC 1 cut(s) 217
MnlI CCTC 5 cut(s) 49, 78, 233, 236, 355
Mox20I TGGCCA 1 cut(s) 311
MscI TGGCCA 1 cut(s) 311
MslI CAYNNNNRTG 1 cut(s) 129
Msp20I TGGCCA 1 cut(s) 311
MspA1I CMGCKG 1 cut(s) 137
MspI CCGG 1 cut(s) 5
Mva1269I GAATGC 1 cut(s) 288
MwoI GCNNNNNNNGC 1 cut(s) 252
NdeII GATC 2 cut(s) 7, 178
NlaIII CATG 4 cut(s) 49, 185, 250, 259
NlaIV GGNNCC 1 cut(s) 366
NmuCI GTSAC 1 cut(s) 52
OliI CACNNNNGTG 1 cut(s) 129
PctI GAATGC 1 cut(s) 288
PkrI GCNGC 2 cut(s) 136, 139
PleI GAGTC 2 cut(s) 73, 339
PpsI GAGTC 2 cut(s) 73, 339
PpuMI RGGWCCY 1 cut(s) 364
Psp5II RGGWCCY 1 cut(s) 364
PspN4I GGNNCC 1 cut(s) 366
PspPI GGNCC 1 cut(s) 364
PspPPI RGGWCCY 1 cut(s) 364
PvuII CAGCTG 1 cut(s) 137
RseI CAYNNNNRTG 1 cut(s) 129
SatI GCNGC 2 cut(s) 135, 138
Sau3AI GATC 2 cut(s) 7, 178
Sau96I GGNCC 1 cut(s) 364
SchI GAGTC 2 cut(s) 73, 339
SduI GDGCHC 2 cut(s) 126, 166
SetI ASST 6 cut(s) 41, 66, 139, 155, 268, 309
SfaNI GCATC 1 cut(s) 365
SinI GGWCC 1 cut(s) 364
SmiMI CAYNNNNRTG 1 cut(s) 129
SsiI CCGC 1 cut(s) 57
SspMI CTAG 3 cut(s) 129, 263, 267
StyI CCWWGG 1 cut(s) 273
TaaI ACNGT 2 cut(s) 18, 219
TseFI GTSAC 1 cut(s) 52
TseI GCWGC 2 cut(s) 134, 137
Tsp45I GTSAC 1 cut(s) 52
TspGWI ACGGA 1 cut(s) 72
VpaK11BI GGWCC 1 cut(s) 364
XspI CTAG 3 cut(s) 129, 263, 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.