RLG00000001467

FAD-dependent monooxygenase required for the C5-ring hydroxylation during ubiquinone biosynthesis. Catalyzes the hydroxylation of 3-polyprenyl-4-hydroxybenzoic acid to 3- polyprenyl-4,5-dihydroxybenzoic acid. The electrons required for the hydroxylation reaction may be funneled indirectly from NADPH via a ferredoxin ferredoxin reductase system to COQ6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006427
Physical Location & Seq
Reverse (-)
15953596 .. 15956222
2627 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001467

Sequence Viewer

Length: 384 bp
ATGTCTAAAGCTCGCGTCTCCGTCGACATCAACGTCCTCCGCCGCAAGGATTTCCGGGACTACGAGTCCTTCATCGTCCAATGGGGAAAGCTTGTCAAAAATGCATACAGATTGAAACTTTCACATCAGGGTTTCTGTACTAGTGAAGCTGCTAAAGTCTCCAGCTCCAATGCAAGAATGCCGATGACGAAGCACTTGAAGGTGGCCATCATTGATAGCAATCCTGTATTATCTAATGGACTCTCAATCAAGAAAGAGGAGCCCCCTGATGCAAGGGTCAGTACAGTAACACCTGTAACCATATCTCCCTTCAAAGGTCAGGAATATTACCATAGCCTGGCTCCCATGTATTATCGCGGTGCAGCTGCAGCTGTTGGTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01100 ko01110 map00130 map01100 map01110
Pfam Domains
Protein Families
DUF1232 FAD_binding_3

Protein Analysis

128

Amino Acids

14.13

Weight (kDa)

9.85

Isoelectric Point (pI)

33.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 32
AccB7I CCANNNNNTGG 1 cut(s) 337
AccI GTMKAC 1 cut(s) 24
AccII CGCG 2 cut(s) 15, 357
AciI CCGC 3 cut(s) 40, 43, 357
AcoI YGGCCR 1 cut(s) 204
AfaI GTAC 2 cut(s) 139, 283
AfiI CCNNNNNNNGG 3 cut(s) 46, 314, 337
AgsI TTSAA 3 cut(s) 115, 199, 313
AhdI GACNNNNNGTC 1 cut(s) 64
AhlI ACTAGT 1 cut(s) 140
AjnI CCWGG 1 cut(s) 336
AluBI AGCT 6 cut(s) 11, 91, 149, 165, 365, 371
AluI AGCT 6 cut(s) 11, 91, 149, 165, 365, 371
Alw26I GTCTC 2 cut(s) 22, 163
AoxI GGCC 1 cut(s) 204
ApeKI GCWGC 4 cut(s) 149, 362, 365, 368
AsuC2I CCSGG 1 cut(s) 56
BalI TGGCCA 1 cut(s) 206
BanII GRGCYC 1 cut(s) 264
BbvI GCAGC 4 cut(s) 136, 352, 374, 380
BccI CCATC 1 cut(s) 215
BciT130I CCWGG 1 cut(s) 338
BcnI CCSGG 1 cut(s) 56
BcoDI GTCTC 2 cut(s) 22, 163
BcuI ACTAGT 1 cut(s) 140
BfaI CTAG 1 cut(s) 141
BfmI CTRYAG 1 cut(s) 366
BisI GCNGC 5 cut(s) 43, 150, 363, 366, 369
BlsI GCNGC 5 cut(s) 44, 151, 364, 367, 370
Bme1390I CCNGG 2 cut(s) 56, 338
BmeRI GACNNNNNGTC 1 cut(s) 64
BmiI GGNNCC 2 cut(s) 261, 342
BmrFI CCNGG 2 cut(s) 56, 338
BmsI GCATC 1 cut(s) 259
BpmI CTGGAG 1 cut(s) 145
BpuMI CCSGG 1 cut(s) 56
BsaBI GATNNNNATC 1 cut(s) 219
BsaXI ACNNNNNCTCC 2 cut(s) 289, 319
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 314, 337
Bse8I GATNNNNATC 1 cut(s) 219
BseBI CCWGG 1 cut(s) 338
BseJI GATNNNNATC 1 cut(s) 219
BseLI CCNNNNNNNGG 3 cut(s) 46, 314, 337
BseRI GAGGAG 1 cut(s) 272
BseXI GCAGC 4 cut(s) 136, 352, 374, 380
BsgI GTGCAG 1 cut(s) 381
Bsh1236I CGCG 2 cut(s) 15, 357
BshFI GGCC 1 cut(s) 206
BsiSI CCGG 1 cut(s) 55
BslFI GGGAC 1 cut(s) 71
BslI CCNNNNNNNGG 3 cut(s) 46, 314, 337
BsmAI GTCTC 2 cut(s) 22, 163
BsmBI CGTCTC 1 cut(s) 22
BsmFI GGGAC 1 cut(s) 71
BsmI GAATGC 1 cut(s) 183
BsnI GGCC 1 cut(s) 206
Bsp1286I GDGCHC 1 cut(s) 264
BspACI CCGC 3 cut(s) 40, 43, 357
BspANI GGCC 1 cut(s) 206
BspFNI CGCG 2 cut(s) 15, 357
BspLI GGNNCC 2 cut(s) 261, 342
BspMAI CTGCAG 1 cut(s) 370
Bst2UI CCWGG 1 cut(s) 338
Bst4CI ACNGT 1 cut(s) 286
BstC8I GCNNGC 1 cut(s) 13
BstFNI CGCG 2 cut(s) 15, 357
BstMAI GTCTC 2 cut(s) 22, 163
BstMWI GCNNNNNNNGC 1 cut(s) 368
BstNI CCWGG 1 cut(s) 338
BstSCI CCNGG 2 cut(s) 54, 336
BstSFI CTRYAG 1 cut(s) 366
BstUI CGCG 2 cut(s) 15, 357
BstV1I GCAGC 4 cut(s) 136, 352, 374, 380
BsuRI GGCC 1 cut(s) 206
Cac8I GCNNGC 1 cut(s) 13
CseI GACGC 1 cut(s) 4
Csp6I GTAC 2 cut(s) 138, 282
CviAII CATG 1 cut(s) 346
CviQI GTAC 2 cut(s) 138, 282
DrdI GACNNNNNNGTC 1 cut(s) 32
DriI GACNNNNNGTC 1 cut(s) 64
DseDI GACNNNNNNGTC 1 cut(s) 32
EaeI YGGCCR 1 cut(s) 204
Eam1105I GACNNNNNGTC 1 cut(s) 64
EciI GGCGGA 1 cut(s) 29
Eco24I GRGCYC 1 cut(s) 264
EcoRII CCWGG 1 cut(s) 336
EcoT22I ATGCAT 1 cut(s) 106
EcoT38I GRGCYC 1 cut(s) 264
Esp3I CGTCTC 1 cut(s) 22
FaeI CATG 1 cut(s) 349
FaiI YATR 5 cut(s) 106, 302, 333, 347, 382
FaqI GGGAC 1 cut(s) 71
FatI CATG 1 cut(s) 345
FblI GTMKAC 1 cut(s) 24
Fnu4HI GCNGC 5 cut(s) 43, 150, 363, 366, 369
FriOI GRGCYC 1 cut(s) 264
Fsp4HI GCNGC 5 cut(s) 43, 150, 363, 366, 369
FspBI CTAG 1 cut(s) 141
GluI GCNGC 5 cut(s) 43, 150, 363, 366, 369
GsuI CTGGAG 1 cut(s) 145
HaeIII GGCC 1 cut(s) 206
HapII CCGG 1 cut(s) 55
HgaI GACGC 1 cut(s) 4
Hin1II CATG 1 cut(s) 349
HincII GTYRAC 1 cut(s) 25
HindII GTYRAC 1 cut(s) 25
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 2 cut(s) 65, 240
HpaII CCGG 1 cut(s) 55
Hpy166II GTNNAC 1 cut(s) 25
Hpy188III TCNNGA 2 cut(s) 250, 320
Hpy8I GTNNAC 1 cut(s) 25
Hpy99I CGWCG 1 cut(s) 26
HpyAV CCTTC 3 cut(s) 79, 193, 319
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4IV ACGT 1 cut(s) 33
HpyCH4V TGCA 5 cut(s) 104, 173, 272, 362, 368
HpyF10VI GCNNNNNNNGC 1 cut(s) 368
HpySE526I ACGT 1 cut(s) 33
Hsp92II CATG 1 cut(s) 349
LmnI GCTCC 3 cut(s) 170, 259, 346
LpnPI CCDG 9 cut(s) 68, 113, 175, 237, 279, 305, 306, 323, 350
Lsp1109I GCAGC 4 cut(s) 136, 352, 374, 380
LweI GCATC 1 cut(s) 259
MaeI CTAG 1 cut(s) 141
MaeII ACGT 1 cut(s) 33
MaeIII GTNAC 2 cut(s) 286, 295
MhlI GDGCHC 1 cut(s) 264
MlsI TGGCCA 1 cut(s) 206
MluNI TGGCCA 1 cut(s) 206
MlyI GAGTC 2 cut(s) 74, 234
MnlI CCTC 2 cut(s) 47, 250
Mox20I TGGCCA 1 cut(s) 206
Mph1103I ATGCAT 1 cut(s) 106
MscI TGGCCA 1 cut(s) 206
Msp20I TGGCCA 1 cut(s) 206
MspA1I CMGCKG 2 cut(s) 365, 371
MspI CCGG 1 cut(s) 55
MspR9I CCNGG 2 cut(s) 56, 338
Mva1269I GAATGC 1 cut(s) 183
MvaI CCWGG 1 cut(s) 338
MvnI CGCG 2 cut(s) 15, 357
MwoI GCNNNNNNNGC 1 cut(s) 368
NciI CCSGG 1 cut(s) 56
NlaIII CATG 1 cut(s) 349
NlaIV GGNNCC 2 cut(s) 261, 342
NsiI ATGCAT 1 cut(s) 106
PcsI WCGNNNNNNNCGW 1 cut(s) 30
PctI GAATGC 1 cut(s) 183
PflMI CCANNNNNTGG 1 cut(s) 337
PfoI TCCNGGA 1 cut(s) 54
PkrI GCNGC 5 cut(s) 44, 151, 364, 367, 370
PleI GAGTC 2 cut(s) 73, 234
PpsI GAGTC 2 cut(s) 73, 234
Psp6I CCWGG 1 cut(s) 336
PspGI CCWGG 1 cut(s) 336
PspN4I GGNNCC 2 cut(s) 261, 342
PstI CTGCAG 1 cut(s) 370
PvuII CAGCTG 2 cut(s) 365, 371
RsaI GTAC 2 cut(s) 139, 283
RsaNI GTAC 2 cut(s) 138, 282
SalI GTCGAC 1 cut(s) 23
SatI GCNGC 5 cut(s) 43, 150, 363, 366, 369
SchI GAGTC 2 cut(s) 74, 234
ScrFI CCNGG 2 cut(s) 56, 338
SduI GDGCHC 1 cut(s) 264
SfaNI GCATC 1 cut(s) 259
SfcI CTRYAG 1 cut(s) 366
SpeI ACTAGT 1 cut(s) 140
SsiI CCGC 3 cut(s) 40, 43, 357
SspI AATATT 1 cut(s) 326
SspMI CTAG 1 cut(s) 141
StyD4I CCNGG 2 cut(s) 54, 336
TaaI ACNGT 1 cut(s) 286
TaiI ACGT 1 cut(s) 36
TaqI TCGA 1 cut(s) 24
TatI WGTACW 2 cut(s) 137, 281
TauI GCSGC 1 cut(s) 45
TseI GCWGC 4 cut(s) 149, 362, 365, 368
TspDTI ATGAA 1 cut(s) 61
TspGWI ACGGA 1 cut(s) 10
Van91I CCANNNNNTGG 1 cut(s) 337
XmiI GTMKAC 1 cut(s) 24
XspI CTAG 1 cut(s) 141
Zsp2I ATGCAT 1 cut(s) 106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.