RLG00000034760

FAD-dependent monooxygenase required for the C5-ring hydroxylation during ubiquinone biosynthesis. Catalyzes the hydroxylation of 3-polyprenyl-4-hydroxybenzoic acid to 3- polyprenyl-4,5-dihydroxybenzoic acid. The electrons required for the hydroxylation reaction may be funneled indirectly from NADPH via a ferredoxin ferredoxin reductase system to COQ6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0006427
Physical Location & Seq
Reverse (-)
53997852 .. 53999598
1747 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034760

Sequence Viewer

Length: 549 bp
ATGTCTAAAGCTTGTGTCTCGGTTGACATTAATGTCCTCCGCTGCAAGGATTTCTGGGACTACAAGTCCTTCATCGTCCAATGGGGAACGGTTGTCAAAAATGCATACAGATTGAAACTTTCACATAATGTGCTAGTGCAGTTGCTAAAGTCTCCAGCTCCAATGGATCATATGGCACAGCAGAAATCTTCAAATAAAAGTGAACCGTATGACATTGCTATTGTTGGAGGAGGCATGGTTGGCATGGCCCTAGCTAGTTCCTTGGCAAGAATGCCGATGTCGAAGCACTTGAAGGTGGCCATCATTGATAGCAATCTTGTATTATCTAATGGACTCTTAATCAAGAAAGAGGACCTCCCTGATGCAAGGCATCATTTTCTCATGTTCATCTACTGGCCATCATGCTACGAGCTCAAATTATATGCTCTTGCAAAGCTGGTTAGCTTAAGTAGAAAGAAACAAATGGTGCACTTGTTTTTCTATAGTGGTACCCTCATAAGCTTGGACATGCGTGTTGTAGTCACAGTGGAAATTGCCAATATGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01100 ko01110 map00130 map01100 map01110
Pfam Domains
Protein Families
DUF1232 FAD_binding_3

Protein Analysis

183

Amino Acids

20.57

Weight (kDa)

9.52

Isoelectric Point (pI)

35.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 547
AasI GACNNNNNNGTC 1 cut(s) 32
Acc65I GGTACC 1 cut(s) 488
AccB1I GGYRCC 1 cut(s) 488
AciI CCGC 1 cut(s) 40
AclWI GGATC 1 cut(s) 174
AcoI YGGCCR 2 cut(s) 297, 395
AfaI GTAC 1 cut(s) 490
AfiI CCNNNNNNNGG 1 cut(s) 46
AflII CTTAAG 1 cut(s) 445
AgsI TTSAA 3 cut(s) 115, 192, 292
AhdI GACNNNNNGTC 1 cut(s) 64
AluBI AGCT 7 cut(s) 11, 158, 254, 412, 436, 444, 501
AluI AGCT 7 cut(s) 11, 158, 254, 412, 436, 444, 501
Alw21I GWGCWC 2 cut(s) 414, 471
Alw26I GTCTC 2 cut(s) 22, 156
Alw44I GTGCAC 1 cut(s) 467
AlwI GGATC 1 cut(s) 174
AoxI GGCC 3 cut(s) 246, 297, 395
ApaLI GTGCAC 1 cut(s) 467
ApeKI GCWGC 1 cut(s) 42
AseI ATTAAT 1 cut(s) 30
Asp718I GGTACC 1 cut(s) 488
AspS9I GGNCC 2 cut(s) 247, 352
AvaII GGWCC 1 cut(s) 352
BaeGI GKGCMC 1 cut(s) 471
BalI TGGCCA 2 cut(s) 299, 397
BanI GGYRCC 1 cut(s) 488
BanII GRGCYC 1 cut(s) 414
Bbv12I GWGCWC 2 cut(s) 414, 471
BbvI GCAGC 1 cut(s) 29
BccI CCATC 2 cut(s) 308, 406
BcoDI GTCTC 2 cut(s) 22, 156
BfaI CTAG 3 cut(s) 134, 251, 255
BfmI CTRYAG 1 cut(s) 481
BfrI CTTAAG 1 cut(s) 445
BisI GCNGC 1 cut(s) 43
BlsI GCNGC 1 cut(s) 44
Bme18I GGWCC 1 cut(s) 352
BmeRI GACNNNNNGTC 1 cut(s) 64
BmgT120I GGNCC 2 cut(s) 247, 352
BmiI GGNNCC 1 cut(s) 490
BmsI GCATC 2 cut(s) 352, 379
BpmI CTGGAG 1 cut(s) 138
BsaBI GATNNNNATC 1 cut(s) 312
BsaJI CCNNGG 1 cut(s) 261
BsaXI ACNNNNNCTCC 2 cut(s) 222, 252
Bsc4I CCNNNNNNNGG 1 cut(s) 46
Bse1I ACTGG 1 cut(s) 398
Bse3DI GCAATG 1 cut(s) 213
Bse8I GATNNNNATC 1 cut(s) 312
BseDI CCNNGG 1 cut(s) 261
BseJI GATNNNNATC 1 cut(s) 312
BseLI CCNNNNNNNGG 1 cut(s) 46
BseMI GCAATG 1 cut(s) 213
BseNI ACTGG 1 cut(s) 398
BseRI GAGGAG 1 cut(s) 243
BseSI GKGCMC 1 cut(s) 471
BseXI GCAGC 1 cut(s) 29
BsgI GTGCAG 1 cut(s) 158
BshFI GGCC 3 cut(s) 248, 299, 397
BshNI GGYRCC 1 cut(s) 488
BsiHKAI GWGCWC 2 cut(s) 414, 471
BslFI GGGAC 1 cut(s) 71
BslI CCNNNNNNNGG 1 cut(s) 46
BsmAI GTCTC 2 cut(s) 22, 156
BsmFI GGGAC 1 cut(s) 71
BsmI GAATGC 1 cut(s) 276
BsnI GGCC 3 cut(s) 248, 299, 397
Bsp1286I GDGCHC 2 cut(s) 414, 471
Bsp143I GATC 1 cut(s) 166
BspACI CCGC 1 cut(s) 40
BspANI GGCC 3 cut(s) 248, 299, 397
BspLI GGNNCC 1 cut(s) 490
BspPI GGATC 1 cut(s) 174
BspT107I GGYRCC 1 cut(s) 488
BspTI CTTAAG 1 cut(s) 445
BsrDI GCAATG 1 cut(s) 213
BsrI ACTGG 1 cut(s) 398
BssECI CCNNGG 1 cut(s) 261
BssMI GATC 1 cut(s) 166
BssT1I CCWWGG 1 cut(s) 261
Bst4CI ACNGT 3 cut(s) 91, 207, 526
BstAFI CTTAAG 1 cut(s) 445
BstKTI GATC 1 cut(s) 169
BstMAI GTCTC 2 cut(s) 22, 156
BstMBI GATC 1 cut(s) 166
BstMWI GCNNNNNNNGC 1 cut(s) 240
BstNSI RCATGY 1 cut(s) 511
BstSFI CTRYAG 1 cut(s) 481
BstSLI GKGCMC 1 cut(s) 471
BstV1I GCAGC 1 cut(s) 29
BsuRI GGCC 3 cut(s) 248, 299, 397
BtsIMutI CAGTG 1 cut(s) 531
Cfr13I GGNCC 2 cut(s) 247, 352
Csp6I GTAC 1 cut(s) 489
CviAII CATG 5 cut(s) 235, 244, 382, 402, 508
CviQI GTAC 1 cut(s) 489
DpnI GATC 1 cut(s) 168
DpnII GATC 1 cut(s) 166
DrdI GACNNNNNNGTC 1 cut(s) 32
DriI GACNNNNNGTC 1 cut(s) 64
DseDI GACNNNNNNGTC 1 cut(s) 32
EaeI YGGCCR 2 cut(s) 297, 395
Eam1105I GACNNNNNGTC 1 cut(s) 64
Ecl136II GAGCTC 1 cut(s) 412
Eco130I CCWWGG 1 cut(s) 261
Eco24I GRGCYC 1 cut(s) 414
Eco47I GGWCC 1 cut(s) 352
Eco53kI GAGCTC 1 cut(s) 412
EcoICRI GAGCTC 1 cut(s) 412
EcoO109I RGGNCCY 1 cut(s) 352
EcoT14I CCWWGG 1 cut(s) 261
EcoT22I ATGCAT 1 cut(s) 106
EcoT38I GRGCYC 1 cut(s) 414
ErhI CCWWGG 1 cut(s) 261
FaeI CATG 5 cut(s) 238, 247, 385, 405, 511
FaqI GGGAC 1 cut(s) 71
FatI CATG 5 cut(s) 234, 243, 381, 401, 507
FauNDI CATATG 1 cut(s) 171
Fnu4HI GCNGC 1 cut(s) 43
FriOI GRGCYC 1 cut(s) 414
Fsp4HI GCNGC 1 cut(s) 43
FspBI CTAG 3 cut(s) 134, 251, 255
GluI GCNGC 1 cut(s) 43
GsuI CTGGAG 1 cut(s) 138
HaeIII GGCC 3 cut(s) 248, 299, 397
Hin1II CATG 5 cut(s) 238, 247, 385, 405, 511
HincII GTYRAC 1 cut(s) 25
HindII GTYRAC 1 cut(s) 25
HindIII AAGCTT 2 cut(s) 9, 499
HinfI GANTC 1 cut(s) 333
Hpy166II GTNNAC 3 cut(s) 25, 203, 469
Hpy188III TCNNGA 1 cut(s) 343
Hpy8I GTNNAC 3 cut(s) 25, 203, 469
HpyAV CCTTC 2 cut(s) 79, 286
HpyCH4III ACNGT 3 cut(s) 91, 207, 526
HpyCH4V TGCA 6 cut(s) 45, 104, 139, 365, 431, 469
HpyF10VI GCNNNNNNNGC 1 cut(s) 240
Hsp92II CATG 5 cut(s) 238, 247, 385, 405, 511
KpnI GGTACC 1 cut(s) 492
Kzo9I GATC 1 cut(s) 166
LmnI GCTCC 1 cut(s) 163
LpnPI CCDG 5 cut(s) 40, 168, 372, 379, 422
Lsp1109I GCAGC 1 cut(s) 29
LweI GCATC 2 cut(s) 352, 379
MaeI CTAG 3 cut(s) 134, 251, 255
MaeIII GTNAC 1 cut(s) 520
MalI GATC 1 cut(s) 168
MboI GATC 1 cut(s) 166
MboII GAAGA 1 cut(s) 180
MhlI GDGCHC 2 cut(s) 414, 471
MlsI TGGCCA 2 cut(s) 299, 397
MluCI AATT 2 cut(s) 416, 531
MluNI TGGCCA 2 cut(s) 299, 397
MlyI GAGTC 1 cut(s) 327
MmeI TCCRAC 1 cut(s) 205
MnlI CCTC 6 cut(s) 47, 221, 224, 343, 365, 503
Mox20I TGGCCA 2 cut(s) 299, 397
Mph1103I ATGCAT 1 cut(s) 106
MscI TGGCCA 2 cut(s) 299, 397
MseI TTAA 3 cut(s) 30, 338, 446
Msp20I TGGCCA 2 cut(s) 299, 397
MspA1I CMGCKG 1 cut(s) 42
MspCI CTTAAG 1 cut(s) 445
Mva1269I GAATGC 1 cut(s) 276
MwoI GCNNNNNNNGC 1 cut(s) 240
NdeI CATATG 1 cut(s) 171
NdeII GATC 1 cut(s) 166
NlaIII CATG 5 cut(s) 238, 247, 385, 405, 511
NlaIV GGNNCC 1 cut(s) 490
NmuCI GTSAC 1 cut(s) 520
NsiI ATGCAT 1 cut(s) 106
NspI RCATGY 1 cut(s) 511
PctI GAATGC 1 cut(s) 276
PkrI GCNGC 1 cut(s) 44
PleI GAGTC 1 cut(s) 327
PpsI GAGTC 1 cut(s) 327
PpuMI RGGWCCY 1 cut(s) 352
PshBI ATTAAT 1 cut(s) 30
PsiI TTATAA 1 cut(s) 547
Psp124BI GAGCTC 1 cut(s) 414
Psp5II RGGWCCY 1 cut(s) 352
PspN4I GGNNCC 1 cut(s) 490
PspPI GGNCC 2 cut(s) 247, 352
PspPPI RGGWCCY 1 cut(s) 352
RsaI GTAC 1 cut(s) 490
RsaNI GTAC 1 cut(s) 489
SacI GAGCTC 1 cut(s) 414
SaqAI TTAA 3 cut(s) 30, 338, 446
SatI GCNGC 1 cut(s) 43
Sau3AI GATC 1 cut(s) 166
Sau96I GGNCC 2 cut(s) 247, 352
SchI GAGTC 1 cut(s) 327
SduI GDGCHC 2 cut(s) 414, 471
SetI ASST 9 cut(s) 13, 160, 256, 297, 357, 414, 438, 446, 503
SfaNI GCATC 2 cut(s) 352, 379
SfcI CTRYAG 1 cut(s) 481
SinI GGWCC 1 cut(s) 352
SmlI CTYRAG 1 cut(s) 445
SmoI CTYRAG 1 cut(s) 445
Sse9I AATT 2 cut(s) 416, 531
SsiI CCGC 1 cut(s) 40
SspMI CTAG 3 cut(s) 134, 251, 255
SstI GAGCTC 1 cut(s) 414
StyI CCWWGG 1 cut(s) 261
TaaI ACNGT 3 cut(s) 91, 207, 526
TaqI TCGA 1 cut(s) 281
TasI AATT 2 cut(s) 416, 531
Tru1I TTAA 3 cut(s) 30, 338, 446
Tru9I TTAA 3 cut(s) 30, 338, 446
TscAI CASTG 1 cut(s) 531
TseFI GTSAC 1 cut(s) 520
TseI GCWGC 1 cut(s) 42
Tsp45I GTSAC 1 cut(s) 520
TspDTI ATGAA 2 cut(s) 61, 376
TspRI CASTG 1 cut(s) 531
Vha464I CTTAAG 1 cut(s) 445
VneI GTGCAC 1 cut(s) 467
VpaK11BI GGWCC 1 cut(s) 352
VspI ATTAAT 1 cut(s) 30
XceI RCATGY 1 cut(s) 511
XspI CTAG 3 cut(s) 134, 251, 255
Zsp2I ATGCAT 1 cut(s) 106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.