Rroxscaffold_4G00282760

FAD-dependent monooxygenase required for the C5-ring hydroxylation during ubiquinone biosynthesis. Catalyzes the hydroxylation of 3-polyprenyl-4-hydroxybenzoic acid to 3- polyprenyl-4,5-dihydroxybenzoic acid. The electrons required for the hydroxylation reaction may be funneled indirectly from NADPH via a ferredoxin ferredoxin reductase system to COQ6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0006427
Physical Location & Seq
Forward (+)
4888496 .. 4892479
3984 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00282760.1

Sequence Viewer

Length: 420 bp
ATGGAAGTAGGTGTTTGGCTCCAAAACCAGTTTGGTTGCAAACGAGTCTCTTTGAGCGTGCGCGAGCGTGCCAAAGACCGTGGGCCCACTCGGATCTCGCCTCCTAATCTCGCGGCTATGTCTAAAGCTCGCGTCTCCATCGACATCAAAGTCCCTCCGCCGCAAGGATTTTCGGGACTACGAATCCTTCATCGTCCAATGGGGGGTTTTTGTACTAGTGCAGCTGCTAGAGTCTCCAGCTCCAATGGTGCTCAACCAAGCAATGATCATGTGGCACAGCAGAAATCTTCAAATAAAAGTGAACCGTATGACATTGCTATTGTTGGAGGAGGCATGGTTGGCATGGCCCTAGCTAGTTCCTTGGGTAGTTGGTCTTTTGTGTATGTTTTCATCACTTCAATTATTTTGTGTGTTGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01100 ko01110 map00130 map01100 map01110
Pfam Domains
Protein Families
DUF1232 FAD_binding_3

Protein Analysis

139

Amino Acids

14.79

Weight (kDa)

10.03

Isoelectric Point (pI)

46.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 149
AccII CGCG 3 cut(s) 63, 113, 132
AciI CCGC 3 cut(s) 113, 158, 161
AclWI GGATC 1 cut(s) 101
AfaI GTAC 1 cut(s) 214
AfiI CCNNNNNNNGG 2 cut(s) 164, 203
AgsI TTSAA 2 cut(s) 291, 399
AhlI ACTAGT 1 cut(s) 215
AluBI AGCT 4 cut(s) 128, 224, 240, 353
AluI AGCT 4 cut(s) 128, 224, 240, 353
Alw21I GWGCWC 1 cut(s) 253
Alw26I GTCTC 3 cut(s) 52, 139, 238
AlwI GGATC 1 cut(s) 101
AoxI GGCC 2 cut(s) 83, 345
ApaI GGGCCC 1 cut(s) 87
ApeKI GCWGC 2 cut(s) 221, 224
AspLEI GCGC 1 cut(s) 63
AspS9I GGNCC 3 cut(s) 83, 84, 346
BaeGI GKGCMC 1 cut(s) 87
BanII GRGCYC 1 cut(s) 87
Bbv12I GWGCWC 1 cut(s) 253
BbvI GCAGC 2 cut(s) 211, 233
BccI CCATC 1 cut(s) 146
BclI TGATCA 1 cut(s) 265
BcoDI GTCTC 3 cut(s) 52, 139, 238
BcuI ACTAGT 1 cut(s) 215
BfaI CTAG 4 cut(s) 216, 228, 350, 354
BisI GCNGC 4 cut(s) 114, 161, 222, 225
BlsI GCNGC 4 cut(s) 115, 162, 223, 226
BmgT120I GGNCC 3 cut(s) 83, 84, 346
BmiI GGNNCC 2 cut(s) 20, 85
BpmI CTGGAG 1 cut(s) 220
BsaJI CCNNGG 2 cut(s) 79, 360
BsaXI ACNNNNNCTCC 2 cut(s) 321, 351
Bsc4I CCNNNNNNNGG 2 cut(s) 164, 203
Bse1I ACTGG 1 cut(s) 28
Bse3DI GCAATG 2 cut(s) 268, 312
BseDI CCNNGG 2 cut(s) 79, 360
BseLI CCNNNNNNNGG 2 cut(s) 164, 203
BseMI GCAATG 2 cut(s) 268, 312
BseNI ACTGG 1 cut(s) 28
BseRI GAGGAG 1 cut(s) 342
BseSI GKGCMC 1 cut(s) 87
BseXI GCAGC 2 cut(s) 211, 233
BsgI GTGCAG 1 cut(s) 240
Bsh1236I CGCG 3 cut(s) 63, 113, 132
BshFI GGCC 2 cut(s) 85, 347
BsiHKAI GWGCWC 1 cut(s) 253
BslFI GGGAC 2 cut(s) 137, 189
BslI CCNNNNNNNGG 2 cut(s) 164, 203
BsmAI GTCTC 3 cut(s) 52, 139, 238
BsmBI CGTCTC 1 cut(s) 139
BsmFI GGGAC 2 cut(s) 137, 189
BsnI GGCC 2 cut(s) 85, 347
Bsp120I GGGCCC 1 cut(s) 83
Bsp1286I GDGCHC 2 cut(s) 87, 253
Bsp143I GATC 2 cut(s) 93, 265
BspACI CCGC 3 cut(s) 113, 158, 161
BspANI GGCC 2 cut(s) 85, 347
BspFNI CGCG 3 cut(s) 63, 113, 132
BspLI GGNNCC 2 cut(s) 20, 85
BspPI GGATC 1 cut(s) 101
BsrDI GCAATG 2 cut(s) 268, 312
BsrI ACTGG 1 cut(s) 28
BssECI CCNNGG 2 cut(s) 79, 360
BssMI GATC 2 cut(s) 93, 265
BssT1I CCWWGG 1 cut(s) 360
Bst4CI ACNGT 2 cut(s) 80, 306
BstC8I GCNNGC 4 cut(s) 59, 65, 69, 130
BstDSI CCRYGG 1 cut(s) 79
BstFNI CGCG 3 cut(s) 63, 113, 132
BstHHI GCGC 1 cut(s) 63
BstKTI GATC 2 cut(s) 96, 268
BstMAI GTCTC 3 cut(s) 52, 139, 238
BstMBI GATC 2 cut(s) 93, 265
BstMWI GCNNNNNNNGC 1 cut(s) 339
BstSLI GKGCMC 1 cut(s) 87
BstUI CGCG 3 cut(s) 63, 113, 132
BstV1I GCAGC 2 cut(s) 211, 233
BstX2I RGATCY 1 cut(s) 93
BstYI RGATCY 1 cut(s) 93
BsuRI GGCC 2 cut(s) 85, 347
BtgI CCRYGG 1 cut(s) 79
Cac8I GCNNGC 4 cut(s) 59, 65, 69, 130
CfoI GCGC 1 cut(s) 63
Cfr13I GGNCC 3 cut(s) 83, 84, 346
CseI GACGC 1 cut(s) 121
Csp6I GTAC 1 cut(s) 213
CspCI CAANNNNNGTGG 2 cut(s) 61, 96
CviAII CATG 3 cut(s) 269, 334, 343
CviJI RGCY 8 cut(s) 19, 85, 116, 128, 224, 240, 347, 353
CviKI_1 RGCY 8 cut(s) 19, 85, 116, 128, 224, 240, 347, 353
CviQI GTAC 1 cut(s) 213
DpnI GATC 2 cut(s) 95, 267
DpnII GATC 2 cut(s) 93, 265
DrdI GACNNNNNNGTC 1 cut(s) 149
DseDI GACNNNNNNGTC 1 cut(s) 149
EciI GGCGGA 1 cut(s) 147
Eco130I CCWWGG 1 cut(s) 360
Eco24I GRGCYC 1 cut(s) 87
EcoT14I CCWWGG 1 cut(s) 360
EcoT38I GRGCYC 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 360
Esp3I CGTCTC 1 cut(s) 139
FaeI CATG 3 cut(s) 272, 337, 346
FaiI YATR 6 cut(s) 119, 270, 309, 335, 344, 384
FaqI GGGAC 2 cut(s) 137, 189
FatI CATG 3 cut(s) 268, 333, 342
FbaI TGATCA 1 cut(s) 265
Fnu4HI GCNGC 4 cut(s) 114, 161, 222, 225
FriOI GRGCYC 1 cut(s) 87
Fsp4HI GCNGC 4 cut(s) 114, 161, 222, 225
FspBI CTAG 4 cut(s) 216, 228, 350, 354
GlaI GCGC 1 cut(s) 62
GluI GCNGC 4 cut(s) 114, 161, 222, 225
GsuI CTGGAG 1 cut(s) 220
HaeIII GGCC 2 cut(s) 85, 347
HgaI GACGC 1 cut(s) 121
HhaI GCGC 1 cut(s) 63
Hin1II CATG 3 cut(s) 272, 337, 346
Hin6I GCGC 1 cut(s) 61
HinP1I GCGC 1 cut(s) 61
HinfI GANTC 3 cut(s) 45, 183, 231
Hpy166II GTNNAC 1 cut(s) 302
Hpy188I TCNGA 1 cut(s) 93
Hpy188III TCNNGA 1 cut(s) 174
Hpy8I GTNNAC 1 cut(s) 302
HpyAV CCTTC 1 cut(s) 197
HpyCH4III ACNGT 2 cut(s) 80, 306
HpyCH4V TGCA 2 cut(s) 39, 221
HpyF10VI GCNNNNNNNGC 1 cut(s) 339
Hsp92II CATG 3 cut(s) 272, 337, 346
HspAI GCGC 1 cut(s) 61
Ksp22I TGATCA 1 cut(s) 265
Kzo9I GATC 2 cut(s) 93, 265
LmnI GCTCC 2 cut(s) 24, 245
LpnPI CCDG 2 cut(s) 41, 250
Lsp1109I GCAGC 2 cut(s) 211, 233
MaeI CTAG 4 cut(s) 216, 228, 350, 354
MalI GATC 2 cut(s) 95, 267
MboI GATC 2 cut(s) 93, 265
MboII GAAGA 1 cut(s) 279
MflI RGATCY 1 cut(s) 93
MhlI GDGCHC 2 cut(s) 87, 253
MluCI AATT 1 cut(s) 399
MlyI GAGTC 2 cut(s) 54, 240
MmeI TCCRAC 1 cut(s) 304
MnlI CCTC 4 cut(s) 111, 165, 320, 323
MspA1I CMGCKG 1 cut(s) 224
MvnI CGCG 3 cut(s) 63, 113, 132
MwoI GCNNNNNNNGC 1 cut(s) 339
NdeII GATC 2 cut(s) 93, 265
NlaIII CATG 3 cut(s) 272, 337, 346
NlaIV GGNNCC 2 cut(s) 20, 85
PfeI GAWTC 1 cut(s) 183
PkrI GCNGC 4 cut(s) 115, 162, 223, 226
PleI GAGTC 2 cut(s) 53, 239
PpsI GAGTC 2 cut(s) 53, 239
PspN4I GGNNCC 2 cut(s) 20, 85
PspOMI GGGCCC 1 cut(s) 83
PspPI GGNCC 3 cut(s) 83, 84, 346
PsuI RGATCY 1 cut(s) 93
PvuII CAGCTG 1 cut(s) 224
RsaI GTAC 1 cut(s) 214
RsaNI GTAC 1 cut(s) 213
SatI GCNGC 4 cut(s) 114, 161, 222, 225
Sau3AI GATC 2 cut(s) 93, 265
Sau96I GGNCC 3 cut(s) 83, 84, 346
SchI GAGTC 2 cut(s) 54, 240
SduI GDGCHC 2 cut(s) 87, 253
SetI ASST 5 cut(s) 13, 130, 226, 242, 355
SpeI ACTAGT 1 cut(s) 215
Sse9I AATT 1 cut(s) 399
SsiI CCGC 3 cut(s) 113, 158, 161
SspMI CTAG 4 cut(s) 216, 228, 350, 354
StyI CCWWGG 1 cut(s) 360
TaaI ACNGT 2 cut(s) 80, 306
TaqI TCGA 1 cut(s) 141
TasI AATT 1 cut(s) 399
TatI WGTACW 1 cut(s) 212
TauI GCSGC 2 cut(s) 116, 163
TfiI GAWTC 1 cut(s) 183
TseI GCWGC 2 cut(s) 221, 224
TspDTI ATGAA 2 cut(s) 179, 379
XcmI CCANNNNNNNNNTGG 1 cut(s) 29
XspI CTAG 4 cut(s) 216, 228, 350, 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.