RchiOBHm_Chr4g0436601

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0004033
Physical Location & Seq
Reverse (-)
59864471 .. 59867325
2855 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40492

Sequence Viewer

Length: 465 bp
ATGGACTTAACCGTTGGATATACCGAAGATATTAACGACGAGGTTACCTTCATTCTAGTGCTTGATCAGCCCTTATCTTCCAATACATACTATGTGATACGCCGTAGTGGGAGGCACAGAAGTTTTTTCTCCCCTCCTAATTTTCGATCTCTCTCCTCTCTAGTGTTTTCAATCTTCTGCGTGACTTCTTTCAATTGTGATGTGGCCAGGGAGGCCACGAAGTTCTTGACTTGGAGGAAGCAGTTCAAGCACGGCCACGGCGACTTCAAACCTAATGTCAACAATGCTCTCAAGATGTTCTTGAAGGCTGCCGAGCTTAGGTCCACGAAGGCGATGGTCGATGCCGGACTCATATTCTGGGAGAGAGGCGACAAGGTTAAGGCGTTTGGGTTGTACCGGGAAGCTGCCGAGGCCGGTGACCGGAATGCGCAGGGCAATTTGGGAATGTGGTATTTGCAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Sel1

Protein Analysis

154

Amino Acids

17.65

Weight (kDa)

8.93

Isoelectric Point (pI)

33.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1262 PF06880 8 - 40 2.5e-09 Protein of unknown function (DUF1262)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 429
AcoI YGGCCR 2 cut(s) 204, 253
AfaI GTAC 1 cut(s) 395
AfiI CCNNNNNNNGG 2 cut(s) 318, 420
AgsI TTSAA 5 cut(s) 171, 193, 247, 268, 304
AjnI CCWGG 1 cut(s) 206
AluBI AGCT 2 cut(s) 316, 404
AluI AGCT 2 cut(s) 316, 404
AoxI GGCC 4 cut(s) 204, 213, 253, 411
ApeKI GCWGC 2 cut(s) 308, 404
ArsI GACNNNNNNTTYG 2 cut(s) 261, 293
Asp700I GAANNNNTTC 1 cut(s) 242
AspLEI GCGC 1 cut(s) 430
AspS9I GGNCC 1 cut(s) 321
AsuC2I CCSGG 1 cut(s) 398
AsuHPI GGTGA 1 cut(s) 428
AvaII GGWCC 1 cut(s) 321
BalI TGGCCA 1 cut(s) 206
BbvI GCAGC 2 cut(s) 295, 391
BccI CCATC 1 cut(s) 328
BceAI ACGGC 3 cut(s) 87, 268, 274
BciT130I CCWGG 1 cut(s) 208
BclI TGATCA 1 cut(s) 64
BcnI CCSGG 1 cut(s) 398
BfaI CTAG 2 cut(s) 56, 161
BglI GCCNNNNNGGC 1 cut(s) 212
BisI GCNGC 2 cut(s) 309, 405
BlsI GCNGC 2 cut(s) 310, 406
Bme1390I CCNGG 2 cut(s) 208, 398
Bme18I GGWCC 1 cut(s) 321
BmgT120I GGNCC 1 cut(s) 321
BmrFI CCNGG 2 cut(s) 208, 398
BmsI GCATC 1 cut(s) 331
Bpu10I CCTNAGC 1 cut(s) 317
BpuEI CTTGAG 1 cut(s) 275
BpuMI CCSGG 1 cut(s) 398
BsaJI CCNNGG 3 cut(s) 207, 256, 408
BsaWI WCCGGW 1 cut(s) 420
Bsc4I CCNNNNNNNGG 2 cut(s) 318, 420
Bse118I RCCGGY 1 cut(s) 413
BseBI CCWGG 1 cut(s) 208
BseDI CCNNGG 3 cut(s) 207, 256, 408
BseLI CCNNNNNNNGG 2 cut(s) 318, 420
BseRI GAGGAG 1 cut(s) 145
BseXI GCAGC 2 cut(s) 295, 391
BshFI GGCC 4 cut(s) 206, 215, 255, 413
BsiSI CCGG 4 cut(s) 345, 397, 414, 421
BslI CCNNNNNNNGG 2 cut(s) 318, 420
BsmI GAATGC 1 cut(s) 430
BsnI GGCC 4 cut(s) 206, 215, 255, 413
Bsp143I GATC 2 cut(s) 64, 146
BspANI GGCC 4 cut(s) 206, 215, 255, 413
BsrFI RCCGGY 1 cut(s) 413
BssAI RCCGGY 1 cut(s) 413
BssECI CCNNGG 3 cut(s) 207, 256, 408
BssMI GATC 2 cut(s) 64, 146
Bst2UI CCWGG 1 cut(s) 208
Bst4CI ACNGT 1 cut(s) 13
BstDEI CTNAG 1 cut(s) 317
BstDSI CCRYGG 1 cut(s) 256
BstEII GGTNACC 2 cut(s) 43, 416
BstHHI GCGC 1 cut(s) 430
BstKTI GATC 2 cut(s) 67, 149
BstMBI GATC 2 cut(s) 64, 146
BstMWI GCNNNNNNNGC 4 cut(s) 67, 212, 247, 410
BstNI CCWGG 1 cut(s) 208
BstPI GGTNACC 2 cut(s) 43, 416
BstSCI CCNGG 2 cut(s) 206, 396
BstV1I GCAGC 2 cut(s) 295, 391
BsuRI GGCC 4 cut(s) 206, 215, 255, 413
BtgI CCRYGG 1 cut(s) 256
BtgZI GCGATG 1 cut(s) 347
CfoI GCGC 1 cut(s) 430
Cfr10I RCCGGY 1 cut(s) 413
Cfr13I GGNCC 1 cut(s) 321
Csp6I GTAC 1 cut(s) 394
CviJI RGCY 8 cut(s) 70, 206, 215, 255, 308, 316, 404, 413
CviKI_1 RGCY 8 cut(s) 70, 206, 215, 255, 308, 316, 404, 413
CviQI GTAC 1 cut(s) 394
DdeI CTNAG 1 cut(s) 317
DpnI GATC 2 cut(s) 66, 148
DpnII GATC 2 cut(s) 64, 146
EaeI YGGCCR 2 cut(s) 204, 253
Eco47I GGWCC 1 cut(s) 321
Eco91I GGTNACC 2 cut(s) 43, 416
EcoO65I GGTNACC 2 cut(s) 43, 416
EcoRII CCWGG 1 cut(s) 206
FaiI YATR 4 cut(s) 21, 88, 93, 353
FalI AAGNNNNNCTT 2 cut(s) 284, 316
FbaI TGATCA 1 cut(s) 64
Fnu4HI GCNGC 2 cut(s) 309, 405
Fsp4HI GCNGC 2 cut(s) 309, 405
FspBI CTAG 2 cut(s) 56, 161
FspI TGCGCA 1 cut(s) 429
GlaI GCGC 1 cut(s) 429
GluI GCNGC 2 cut(s) 309, 405
HaeIII GGCC 4 cut(s) 206, 215, 255, 413
HapII CCGG 4 cut(s) 345, 397, 414, 421
HhaI GCGC 1 cut(s) 430
Hin6I GCGC 1 cut(s) 428
HinP1I GCGC 1 cut(s) 428
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HinfI GANTC 1 cut(s) 348
HpaII CCGG 4 cut(s) 345, 397, 414, 421
HphI GGTGA 1 cut(s) 428
Hpy166II GTNNAC 2 cut(s) 280, 324
Hpy188III TCNNGA 3 cut(s) 226, 292, 301
Hpy8I GTNNAC 2 cut(s) 280, 324
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 3 cut(s) 58, 298, 322
HpyCH4III ACNGT 1 cut(s) 13
HpyCH4V TGCA 1 cut(s) 457
HpyF10VI GCNNNNNNNGC 4 cut(s) 67, 212, 247, 410
HpyF3I CTNAG 1 cut(s) 317
HspAI GCGC 1 cut(s) 428
Ksp22I TGATCA 1 cut(s) 64
Kzo9I GATC 2 cut(s) 64, 146
LpnPI CCDG 8 cut(s) 193, 220, 343, 358, 410, 416, 427, 434
Lsp1109I GCAGC 2 cut(s) 295, 391
LweI GCATC 1 cut(s) 331
MaeI CTAG 2 cut(s) 56, 161
MaeIII GTNAC 3 cut(s) 43, 181, 416
MalI GATC 2 cut(s) 66, 148
MboI GATC 2 cut(s) 64, 146
MboII GAAGA 3 cut(s) 38, 69, 166
MfeI CAATTG 1 cut(s) 193
MlsI TGGCCA 1 cut(s) 206
MluCI AATT 3 cut(s) 139, 193, 436
MluNI TGGCCA 1 cut(s) 206
MlyI GAGTC 1 cut(s) 342
MnlI CCTC 8 cut(s) 34, 105, 144, 166, 205, 228, 359, 403
Mox20I TGGCCA 1 cut(s) 206
MroXI GAANNNNTTC 1 cut(s) 242
MscI TGGCCA 1 cut(s) 206
MseI TTAA 3 cut(s) 8, 33, 378
MslI CAYNNNNRTG 1 cut(s) 56
Msp20I TGGCCA 1 cut(s) 206
MspI CCGG 4 cut(s) 345, 397, 414, 421
MspR9I CCNGG 2 cut(s) 208, 398
MunI CAATTG 1 cut(s) 193
Mva1269I GAATGC 1 cut(s) 430
MvaI CCWGG 1 cut(s) 208
MwoI GCNNNNNNNGC 4 cut(s) 67, 212, 247, 410
NciI CCSGG 1 cut(s) 398
NdeII GATC 2 cut(s) 64, 146
NmeAIII GCCGAG 2 cut(s) 337, 433
NmuCI GTSAC 2 cut(s) 181, 416
NsbI TGCGCA 1 cut(s) 429
PcsI WCGNNNNNNNCGW 1 cut(s) 258
PctI GAATGC 1 cut(s) 430
PdmI GAANNNNTTC 1 cut(s) 242
PkrI GCNGC 2 cut(s) 310, 406
PleI GAGTC 1 cut(s) 342
PpsI GAGTC 1 cut(s) 342
Psp6I CCWGG 1 cut(s) 206
PspEI GGTNACC 2 cut(s) 43, 416
PspGI CCWGG 1 cut(s) 206
PspPI GGNCC 1 cut(s) 321
RsaI GTAC 1 cut(s) 395
RsaNI GTAC 1 cut(s) 394
RseI CAYNNNNRTG 1 cut(s) 56
SaqAI TTAA 3 cut(s) 8, 33, 378
SatI GCNGC 2 cut(s) 309, 405
Sau3AI GATC 2 cut(s) 64, 146
Sau96I GGNCC 1 cut(s) 321
SchI GAGTC 1 cut(s) 342
ScrFI CCNGG 2 cut(s) 208, 398
SetI ASST 7 cut(s) 45, 50, 274, 318, 323, 378, 406
SfaNI GCATC 1 cut(s) 331
SfiI GGCCNNNNNGGCC 1 cut(s) 212
SinI GGWCC 1 cut(s) 321
SmiMI CAYNNNNRTG 1 cut(s) 56
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
Sse9I AATT 3 cut(s) 139, 193, 436
SspMI CTAG 2 cut(s) 56, 161
StyD4I CCNGG 2 cut(s) 206, 396
TaaI ACNGT 1 cut(s) 13
TaqI TCGA 2 cut(s) 145, 339
TasI AATT 3 cut(s) 139, 193, 436
Tru1I TTAA 3 cut(s) 8, 33, 378
Tru9I TTAA 3 cut(s) 8, 33, 378
TseFI GTSAC 2 cut(s) 181, 416
TseI GCWGC 2 cut(s) 308, 404
Tsp45I GTSAC 2 cut(s) 181, 416
TspDTI ATGAA 1 cut(s) 40
VpaK11BI GGWCC 1 cut(s) 321
XcmI CCANNNNNNNNNTGG 1 cut(s) 331
XmnI GAANNNNTTC 1 cut(s) 242
XspI CTAG 2 cut(s) 56, 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.