RchiOBHm_Chr5g0006921

Splicing factor 3B subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0007554
Physical Location & Seq
Forward (+)
4285773 .. 4286903
1131 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28802

Sequence Viewer

Length: 345 bp
ATGCAACTGCTTTTCTTTCTGAAATTGTATCGGAATACTGTACTGGAGCCAAGGCATTGGTGCCAGAACAGGAAACTTCTACAGCGATGTGGAGCCTGGACTTGTAGGAGTTTGGGTAAACGTGGTACCGGAAAACAACCGTTTCAGCTTCCTGATTTCATTGCTGCAACTGGAATTGAGAAAATTAGACAGGCATACATTGAAAAAGAAGATAGTAAGAAGTCGAAGCAAGTGCACCAAGAATGTATGCAGCCAAAGATGGCAAAACAGATATTGATTATCAGGTTTGCCTTTAATATATTTGGCATCAAGTCTTTGGACTCTTATTTTAATGCTCTTCCGTAA

Protein Analysis

114

Amino Acids

13.39

Weight (kDa)

9.98

Isoelectric Point (pI)

52.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF382 PF04037 3 - 92 3.3e-18 Domain of unknown function (DUF382)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 125
AccB1I GGYRCC 2 cut(s) 60, 125
AfaI GTAC 2 cut(s) 42, 127
AgsI TTSAA 1 cut(s) 203
AjnI CCWGG 1 cut(s) 95
AluBI AGCT 1 cut(s) 148
AluI AGCT 1 cut(s) 148
Alw21I GWGCWC 1 cut(s) 237
Alw44I GTGCAC 1 cut(s) 233
ApaLI GTGCAC 1 cut(s) 233
ApeKI GCWGC 2 cut(s) 164, 250
Asp718I GGTACC 1 cut(s) 125
BaeGI GKGCMC 1 cut(s) 237
BanI GGYRCC 2 cut(s) 60, 125
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 2 cut(s) 151, 262
BccI CCATC 1 cut(s) 253
BcgI CGANNNNNNTGC 2 cut(s) 214, 248
BciT130I CCWGG 1 cut(s) 97
BfmI CTRYAG 1 cut(s) 80
BisI GCNGC 2 cut(s) 165, 251
BlsI GCNGC 2 cut(s) 166, 252
Bme1390I CCNGG 1 cut(s) 97
BmiI GGNNCC 4 cut(s) 48, 62, 94, 127
BmrFI CCNGG 1 cut(s) 97
BmsI GCATC 1 cut(s) 315
BpmI CTGGAG 1 cut(s) 65
BsaJI CCNNGG 1 cut(s) 50
BsaWI WCCGGW 1 cut(s) 128
BsaXI ACNNNNNCTCC 2 cut(s) 100, 130
Bse1I ACTGG 2 cut(s) 48, 175
Bse3DI GCAATG 1 cut(s) 159
BseBI CCWGG 1 cut(s) 97
BseDI CCNNGG 1 cut(s) 50
BseMI GCAATG 1 cut(s) 159
BseNI ACTGG 2 cut(s) 48, 175
BseSI GKGCMC 1 cut(s) 237
BseXI GCAGC 2 cut(s) 151, 262
BshNI GGYRCC 2 cut(s) 60, 125
BsiHKAI GWGCWC 1 cut(s) 237
BsiSI CCGG 1 cut(s) 129
Bsp1286I GDGCHC 1 cut(s) 237
BspLI GGNNCC 4 cut(s) 48, 62, 94, 127
BspQI GCTCTTC 1 cut(s) 342
BspT107I GGYRCC 2 cut(s) 60, 125
BsrDI GCAATG 1 cut(s) 159
BsrI ACTGG 2 cut(s) 48, 175
BssECI CCNNGG 1 cut(s) 50
BssT1I CCWWGG 1 cut(s) 50
Bst2UI CCWGG 1 cut(s) 97
Bst4CI ACNGT 2 cut(s) 40, 141
Bst6I CTCTTC 1 cut(s) 342
BstNI CCWGG 1 cut(s) 97
BstSCI CCNGG 1 cut(s) 95
BstSFI CTRYAG 1 cut(s) 80
BstSLI GKGCMC 1 cut(s) 237
BstV1I GCAGC 2 cut(s) 151, 262
BstXI CCANNNNNNTGG 1 cut(s) 57
BtgZI GCGATG 1 cut(s) 100
Csp6I GTAC 2 cut(s) 41, 126
CviJI RGCY 4 cut(s) 49, 95, 148, 253
CviKI_1 RGCY 4 cut(s) 49, 95, 148, 253
CviQI GTAC 2 cut(s) 41, 126
Eam1104I CTCTTC 1 cut(s) 342
EarI CTCTTC 1 cut(s) 342
Eco130I CCWWGG 1 cut(s) 50
EcoRII CCWGG 1 cut(s) 95
EcoT14I CCWWGG 1 cut(s) 50
ErhI CCWWGG 1 cut(s) 50
FaiI YATR 3 cut(s) 196, 248, 299
Fnu4HI GCNGC 2 cut(s) 165, 251
Fsp4HI GCNGC 2 cut(s) 165, 251
GluI GCNGC 2 cut(s) 165, 251
GsuI CTGGAG 1 cut(s) 65
HapII CCGG 1 cut(s) 129
HinfI GANTC 1 cut(s) 320
HpaII CCGG 1 cut(s) 129
Hpy166II GTNNAC 2 cut(s) 119, 235
Hpy188I TCNGA 2 cut(s) 21, 33
Hpy188III TCNNGA 1 cut(s) 152
Hpy8I GTNNAC 2 cut(s) 119, 235
HpyCH4III ACNGT 2 cut(s) 40, 141
HpyCH4IV ACGT 1 cut(s) 121
HpyCH4V TGCA 4 cut(s) 4, 167, 235, 250
HpySE526I ACGT 1 cut(s) 121
KpnI GGTACC 1 cut(s) 129
LguI GCTCTTC 1 cut(s) 342
LmnI GCTCC 2 cut(s) 46, 92
Lsp1109I GCAGC 2 cut(s) 151, 262
LweI GCATC 1 cut(s) 315
MaeII ACGT 1 cut(s) 121
MboII GAAGA 2 cut(s) 221, 329
MhlI GDGCHC 1 cut(s) 237
MluCI AATT 3 cut(s) 23, 174, 183
MlyI GAGTC 1 cut(s) 314
MseI TTAA 2 cut(s) 294, 330
MspI CCGG 1 cut(s) 129
MspR9I CCNGG 1 cut(s) 97
MvaI CCWGG 1 cut(s) 97
NlaIV GGNNCC 4 cut(s) 48, 62, 94, 127
PciSI GCTCTTC 1 cut(s) 342
PkrI GCNGC 2 cut(s) 166, 252
PleI GAGTC 1 cut(s) 314
PpsI GAGTC 1 cut(s) 314
Psp6I CCWGG 1 cut(s) 95
PspGI CCWGG 1 cut(s) 95
PspN4I GGNNCC 4 cut(s) 48, 62, 94, 127
RsaI GTAC 2 cut(s) 42, 127
RsaNI GTAC 2 cut(s) 41, 126
SapI GCTCTTC 1 cut(s) 342
SaqAI TTAA 2 cut(s) 294, 330
SatI GCNGC 2 cut(s) 165, 251
SchI GAGTC 1 cut(s) 314
ScrFI CCNGG 1 cut(s) 97
SduI GDGCHC 1 cut(s) 237
SetI ASST 3 cut(s) 124, 150, 287
SfaNI GCATC 1 cut(s) 315
SfcI CTRYAG 1 cut(s) 80
Sse9I AATT 3 cut(s) 23, 174, 183
StyD4I CCNGG 1 cut(s) 95
StyI CCWWGG 1 cut(s) 50
TaaI ACNGT 2 cut(s) 40, 141
TaiI ACGT 1 cut(s) 124
TaqI TCGA 1 cut(s) 224
TasI AATT 3 cut(s) 23, 174, 183
TatI WGTACW 1 cut(s) 40
Tru1I TTAA 2 cut(s) 294, 330
Tru9I TTAA 2 cut(s) 294, 330
TseI GCWGC 2 cut(s) 164, 250
TspDTI ATGAA 1 cut(s) 148
TspGWI ACGGA 1 cut(s) 330
VneI GTGCAC 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.