RLG00000031441

Splicing factor 3B subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0007554
Physical Location & Seq
Forward (+)
5178490 .. 5181779
3290 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031441

Sequence Viewer

Length: 687 bp
ATGTTGTTGAGGTGTGGGATGCAACTGCTTTTCTTTCTAAAATTGTATCGGAATACTGTACTGGAGCCAAGGCATTGGTGCCAGAACAGGAAATTTCTACAGGGTAAACGTGGTACCGGAAAACAACCGTTTCAGCTTCCTGATTTCATTGCTGCAACTGGAATTGAGAAAATTAGACAGGTAAATTGGCATACATTGAAAAATGAAGATGGTAAGAAGTCGAAGCAAGTGCAACAAGAATCACCAAATATGCCAGAGCTCACAACACTTGGTGATCTGTACCATGAAGGGAAAGAGTTTGAGGAAATATTAAGAGAGATGAAGCCAGAATTGCTGTCATATGAACTGAAAGAAGCTCTTGTTCTCTTTAGCTCTGCATTGTATGGAGACGTGTTTGGTGTTCAGCAACAAGATCAGCCTAATTATGAGGAAAAGCCAGTTGATAAGACAAAGCATTGGGGTGATTTGGAGAGAGAGGAAGAAGAGGAAGAACCGATGGAGGGCAAAGATCTAGAAGATGGTATTGAATCTGTAGATAGCCTTTCAAGACTTCCGCACTTCCTTCTCAGTACTCCCACTGGCAATGAGAAACCTGATGTCATTGACCTTCACAAGCAGCAGAGAAAGGAGCCTGAAAGACCTTTATACCAAGTAAGTACGATGGCAGACACTTTCTTTAACGACTAA

Protein Analysis

229

Amino Acids

26.67

Weight (kDa)

5.08

Isoelectric Point (pI)

46.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF382 PF04037 9 - 80 4.1e-18 Domain of unknown function (DUF382)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 113
AccB1I GGYRCC 2 cut(s) 78, 113
AciI CCGC 1 cut(s) 554
AcsI RAATTY 1 cut(s) 92
AdeI CACNNNGTG 1 cut(s) 272
AfaI GTAC 5 cut(s) 60, 115, 281, 571, 658
AfiI CCNNNNNNNGG 1 cut(s) 500
AflIII ACRYGT 1 cut(s) 390
AgsI TTSAA 3 cut(s) 199, 527, 546
AjiI CACGTC 1 cut(s) 391
AluBI AGCT 4 cut(s) 136, 259, 356, 372
AluI AGCT 4 cut(s) 136, 259, 356, 372
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 1 cut(s) 381
ApeKI GCWGC 2 cut(s) 152, 616
ApoI RAATTY 1 cut(s) 92
Asp718I GGTACC 1 cut(s) 113
AsuHPI GGTGA 3 cut(s) 234, 284, 473
BanI GGYRCC 2 cut(s) 78, 113
BanII GRGCYC 1 cut(s) 261
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 2 cut(s) 139, 628
BccI CCATC 4 cut(s) 203, 490, 512, 655
BcgI CGANNNNNNTGC 2 cut(s) 211, 245
BcoDI GTCTC 1 cut(s) 381
BfaI CTAG 1 cut(s) 512
BfmI CTRYAG 2 cut(s) 98, 531
BglII AGATCT 1 cut(s) 508
BisI GCNGC 2 cut(s) 153, 617
BlsI GCNGC 2 cut(s) 154, 618
BmcAI AGTACT 1 cut(s) 571
BmgBI CACGTC 1 cut(s) 391
BmiI GGNNCC 4 cut(s) 66, 80, 115, 630
BmsI GCATC 1 cut(s) 9
BpmI CTGGAG 1 cut(s) 83
BsaJI CCNNGG 1 cut(s) 68
BsaWI WCCGGW 1 cut(s) 116
Bsc4I CCNNNNNNNGG 1 cut(s) 500
Bse1I ACTGG 4 cut(s) 66, 163, 437, 583
Bse3DI GCAATG 2 cut(s) 147, 589
BseDI CCNNGG 1 cut(s) 68
BseGI GGATG 1 cut(s) 24
BseLI CCNNNNNNNGG 1 cut(s) 500
BseMI GCAATG 2 cut(s) 147, 589
BseMII CTCAG 1 cut(s) 580
BseNI ACTGG 4 cut(s) 66, 163, 437, 583
BseXI GCAGC 2 cut(s) 139, 628
BshNI GGYRCC 2 cut(s) 78, 113
BsiHKAI GWGCWC 1 cut(s) 261
BsiSI CCGG 1 cut(s) 117
BslI CCNNNNNNNGG 1 cut(s) 500
BsmAI GTCTC 1 cut(s) 381
BsmBI CGTCTC 1 cut(s) 381
Bsp1286I GDGCHC 1 cut(s) 261
Bsp143I GATC 3 cut(s) 274, 412, 508
BspACI CCGC 1 cut(s) 554
BspCNI CTCAG 1 cut(s) 579
BspLI GGNNCC 4 cut(s) 66, 80, 115, 630
BspT107I GGYRCC 2 cut(s) 78, 113
BsrDI GCAATG 2 cut(s) 147, 589
BsrI ACTGG 4 cut(s) 66, 163, 437, 583
BssECI CCNNGG 1 cut(s) 68
BssMI GATC 3 cut(s) 274, 412, 508
BssT1I CCWWGG 1 cut(s) 68
Bst4CI ACNGT 2 cut(s) 58, 129
Bst6I CTCTTC 1 cut(s) 477
BstDEI CTNAG 1 cut(s) 566
BstF5I GGATG 1 cut(s) 24
BstKTI GATC 3 cut(s) 277, 415, 511
BstMAI GTCTC 1 cut(s) 381
BstMBI GATC 3 cut(s) 274, 412, 508
BstMWI GCNNNNNNNGC 1 cut(s) 331
BstSFI CTRYAG 2 cut(s) 98, 531
BstV1I GCAGC 2 cut(s) 139, 628
BstX2I RGATCY 1 cut(s) 508
BstXI CCANNNNNNTGG 1 cut(s) 75
BstYI RGATCY 1 cut(s) 508
BtrI CACGTC 1 cut(s) 391
BtsCI GGATG 1 cut(s) 24
BtsIMutI CAGTG 1 cut(s) 576
Csp6I GTAC 5 cut(s) 59, 114, 280, 570, 657
CviAII CATG 1 cut(s) 284
CviQI GTAC 5 cut(s) 59, 114, 280, 570, 657
DdeI CTNAG 1 cut(s) 566
DpnI GATC 3 cut(s) 276, 414, 510
DpnII GATC 3 cut(s) 274, 412, 508
DraIII CACNNNGTG 1 cut(s) 272
Eam1104I CTCTTC 1 cut(s) 477
EarI CTCTTC 1 cut(s) 477
Ecl136II GAGCTC 1 cut(s) 259
Eco130I CCWWGG 1 cut(s) 68
Eco24I GRGCYC 1 cut(s) 261
Eco53kI GAGCTC 1 cut(s) 259
EcoICRI GAGCTC 1 cut(s) 259
EcoT14I CCWWGG 1 cut(s) 68
EcoT38I GRGCYC 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 68
Esp3I CGTCTC 1 cut(s) 381
FaeI CATG 1 cut(s) 287
FaiI YATR 8 cut(s) 192, 251, 285, 340, 342, 384, 426, 646
FalI AAGNNNNNCTT 2 cut(s) 342, 374
FatI CATG 1 cut(s) 283
FauNDI CATATG 1 cut(s) 340
Fnu4HI GCNGC 2 cut(s) 153, 617
FokI GGATG 1 cut(s) 31
FriOI GRGCYC 1 cut(s) 261
Fsp4HI GCNGC 2 cut(s) 153, 617
FspBI CTAG 1 cut(s) 512
GluI GCNGC 2 cut(s) 153, 617
GsuI CTGGAG 1 cut(s) 83
HapII CCGG 1 cut(s) 117
Hin1II CATG 1 cut(s) 287
HinfI GANTC 2 cut(s) 239, 527
HpaII CCGG 1 cut(s) 117
HphI GGTGA 3 cut(s) 234, 284, 473
Hpy166II GTNNAC 1 cut(s) 107
Hpy188I TCNGA 1 cut(s) 51
Hpy188III TCNNGA 3 cut(s) 140, 512, 546
Hpy8I GTNNAC 1 cut(s) 107
HpyAV CCTTC 3 cut(s) 281, 572, 617
HpyCH4III ACNGT 2 cut(s) 58, 129
HpyCH4IV ACGT 2 cut(s) 109, 390
HpyCH4V TGCA 4 cut(s) 22, 155, 232, 377
HpyF10VI GCNNNNNNNGC 1 cut(s) 331
HpyF3I CTNAG 1 cut(s) 566
HpySE526I ACGT 2 cut(s) 109, 390
Hsp92II CATG 1 cut(s) 287
KpnI GGTACC 1 cut(s) 117
Kzo9I GATC 3 cut(s) 274, 412, 508
LmnI GCTCC 2 cut(s) 64, 628
Lsp1109I GCAGC 2 cut(s) 139, 628
LweI GCATC 1 cut(s) 9
MaeI CTAG 1 cut(s) 512
MaeII ACGT 2 cut(s) 109, 390
MalI GATC 3 cut(s) 276, 414, 510
MboI GATC 3 cut(s) 274, 412, 508
MboII GAAGA 5 cut(s) 218, 491, 494, 500, 527
MflI RGATCY 1 cut(s) 508
MhlI GDGCHC 1 cut(s) 261
MluCI AATT 7 cut(s) 41, 92, 162, 171, 184, 329, 421
MnlI CCTC 6 cut(s) 3, 295, 421, 469, 478, 493
MseI TTAA 2 cut(s) 311, 678
MslI CAYNNNNRTG 1 cut(s) 459
MspI CCGG 1 cut(s) 117
MwoI GCNNNNNNNGC 1 cut(s) 331
NdeI CATATG 1 cut(s) 340
NdeII GATC 3 cut(s) 274, 412, 508
NlaIII CATG 1 cut(s) 287
NlaIV GGNNCC 4 cut(s) 66, 80, 115, 630
PfeI GAWTC 2 cut(s) 239, 527
PkrI GCNGC 2 cut(s) 154, 618
Psp124BI GAGCTC 1 cut(s) 261
PspN4I GGNNCC 4 cut(s) 66, 80, 115, 630
PsuI RGATCY 1 cut(s) 508
RsaI GTAC 5 cut(s) 60, 115, 281, 571, 658
RsaNI GTAC 5 cut(s) 59, 114, 280, 570, 657
RseI CAYNNNNRTG 1 cut(s) 459
SacI GAGCTC 1 cut(s) 261
SaqAI TTAA 2 cut(s) 311, 678
SatI GCNGC 2 cut(s) 153, 617
Sau3AI GATC 3 cut(s) 274, 412, 508
ScaI AGTACT 1 cut(s) 571
SduI GDGCHC 1 cut(s) 261
SfaNI GCATC 1 cut(s) 9
SfcI CTRYAG 2 cut(s) 98, 531
SmiMI CAYNNNNRTG 1 cut(s) 459
Sse9I AATT 7 cut(s) 41, 92, 162, 171, 184, 329, 421
SsiI CCGC 1 cut(s) 554
SspI AATATT 1 cut(s) 309
SspMI CTAG 1 cut(s) 512
SstI GAGCTC 1 cut(s) 261
StyI CCWWGG 1 cut(s) 68
TaaI ACNGT 2 cut(s) 58, 129
TaiI ACGT 2 cut(s) 112, 393
TaqI TCGA 1 cut(s) 221
TasI AATT 7 cut(s) 41, 92, 162, 171, 184, 329, 421
TatI WGTACW 2 cut(s) 58, 569
TfiI GAWTC 2 cut(s) 239, 527
Tru1I TTAA 2 cut(s) 311, 678
Tru9I TTAA 2 cut(s) 311, 678
TscAI CASTG 1 cut(s) 583
TseI GCWGC 2 cut(s) 152, 616
TspDTI ATGAA 5 cut(s) 136, 219, 300, 335, 357
TspRI CASTG 1 cut(s) 583
XapI RAATTY 1 cut(s) 92
XbaI TCTAGA 1 cut(s) 511
XspI CTAG 1 cut(s) 512
ZrmI AGTACT 1 cut(s) 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.