Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0004551

Physical Location & Seq

Genomic Coordinates Reverse (-)

56751528 .. 56756713

Gene Structure 5186 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ33143

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2487 bp

ATGGCGGAGTCTACTGAGCATCAGCAACTGGCGGCCATTCTCGGTCCTGAGCCCGCCGCCACTTTCCAAACCGTAATCTCCCACTTAATCTCCTCCCCCTCCTCTTTGCAAACCCTAATCTCCGACCTCATCTCCTCCGAGAAGCGGTCCGAGGCCGAGCTCGTCTTCAACATCTGCAAGCAAGCCGATCACGAGTCGCTTTCTCTCAAACTCGCTCAGCTTCTCCAGGTCTCTCCCGACGATAAGACCCGAGCCATGTGCTCCATTCTCCTCCGGAAGCAACTACTCACCGGTGACCTGTGGCGAAGACTCTCTCCTAACACCATATCCACCCTCAAACATACTCTATTATCTTGTATTCAACGCGAGGACACCCGAACATCGATTTCGAAGGCCATCTGCTACGCCGTGTCGGAGCTCGCCTCCGCAGAGTTAGAGTTGTTGGGCGGCGAAGGCTGGCCGGAGCTCTTGCCCTTTCAGTTCCAATCCACCTCCTACGATGCGCCGCCGAAGCTTCAGGAATGCTCCTTCTTGATTTTCGATCAGCTCGCGGACTTCTTTACCCCGTTTATAGACGAATTACACACCGTCTTCTTATACTGCTTGAGCTCCTCCACAAGGTCAAATGAAGTTAAGATAGCCGCGTTTAGTGCGGTGGTCAGTTTCATTCGGTGTTTGTCCAGCTCTGAAGATCCGAATATGTTTCACGACGTCTTGATAGAAATGATGAGAACAGTGATGGGGGAATTGAACAAAGGGAAGGAGATCATGGCACAGGAAGCGATCAAGTTGTTCATTGAATTGGCCGGGACCCATCCGACGTTGCTCAGCCGCAACGGGCAGATCATCCGGGCTATGCTGCAGATTGCTGAAGCCATAGATGTCTTGGAACAAGGGACGAGGCACTTGGCCATTGAGTTTCTGATTACTCTGGCCGAGGCCGGAGAGCGTGCACCATGGATAATGAGGAGGTGTCGGGAGTTTGTTGGTCTACTTTTTCCCATTTTAATGAGGATGGTTTCAAATGTCAAGGATGATCCCTCATGGCATACAGCTGAGACTGATGATGACATTGCCTTGGGTGTAAGTGGTGACTACTGTGTTGGGCAGGAGTGTTTGCATAGGCTTGCCATTGCCTTGGGTGGCAATAACATTGTTCCAATTGCGTTAGAACACTTTACTTCCCATTTTGCTGCACCCGAGTGGCCCAAGCATCATGCAGCATTGATTGCCCTTGCTCAAATCGCTGAGGGTTGCTCTGAGATAATGATAAAAGATTTGGAGCAAGTGGTGGCAATGGTTTTGAACTCCCTTGAACATCCTCATATTCGGGTAAGGTGGTCAGCTATTAATGCAGTTGGCCAGTTGTCTACTTATTTGGCCCCAGACCTGCAAGTTCAATATCATCAACAGGTGTTTTCTGCACTGAATGCCACTATATATGGTTTGCAGAACCGTCATCTACTGGCTTGTGCTGCTTCAGCACTGCAAAAATTCATTGAGAAATGTGCTAGTGATATCTTGACACCTTATTTGGATGAAGTGGTTTCCCAATTGGTTGTACTTTTGCAGAGTGAGATACAAATGGTGCATGTAGAAGCATTGACTGCTTTGGCATCAGTCGTTGTTTCATTTCCGGACCAATTCCAAAAATACTATGAAGATGTCATGATTCCCCTGAGAGGTATGTTGTTGGATGCCACTAATAACCCGGTTTGGTCCAAGTCCGTGGAGTGTATTAGCCTGGTTGGTATGGCTGTTGGAAAGGAGAAATTCAGGGATGATGCTAAGAAAGTCATGGAAGTGCTGATGTCTTTTCAAGGATCTCAAATGGAGCTAGATGACCCAACTGCAAGTTGCATGCTACAGATATGCGCTGACCTCTGCAAGTGTCTAGGACAAGATTTCCTTCCATACATGGGTGCTGTTATGCCTCCTTTGCTTCAGTTTGTTCAAATTAAGCCTGTTGAAACCGTTACCTCCGCAAATGACAATAGTGATATGGATGAATCTGATGATGAAAGTATGGAAACAATTACTCTTGGAGATAAAAGGATTAGAATCAAGACTAGTGTGTTGGAGGAAAAAGTTACAGCTTGCAATTTGCTGTGTTGCTATGCTGATGAACTGAAGGAAGGATTCTTTCCATGGATCGATCGGGTTGCTCCAACGTTAGTTCCACTTATATCTTATATCCATGAAAAAGTGAGGAAGTCTGCTGTTTCAGCAATGCCGAAGCTACTGCTTTCTGCAAAGTTAGCTATAGAGAAAAGACAATCCCAAGGATGTAATGAAACCTATATAAAGAAATTGTCTGACCACATCCTTCCAGCTCTGGTGGAAGCATCACATCAGGAAACTGATAGAGAAATCACTGCAATAATATTGGATGCTTTAAATGAATGCATACAGATATCTGGACCACTTCGAGATGAAGCCATGTCACATGCACCTAGTCGAGGTGATAAAGCAGGGATCACTCCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000060 GO:0001101 GO:0003674 GO:0005048 GO:0005215 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008139 GO:0008150 GO:0008565 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0017038 GO:0031090 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032386 GO:0032388 GO:0032879 GO:0032880 GO:0033036 GO:0033157 GO:0033218 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0042221 GO:0042277 GO:0042306 GO:0042307 GO:0042886 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046822 GO:0046824 GO:0046907 GO:0048518 GO:0050789 GO:0050896 GO:0051049 GO:0051050 GO:0051169 GO:0051170 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0060341 GO:0065007 GO:0070013 GO:0070201 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071702 GO:0071705 GO:0072594 GO:0090087 GO:0090316 GO:1900180 GO:1900182 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1903827 GO:1903829 GO:1904589 GO:1904591 GO:1904951

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HEAT HEAT_2 HEAT_EZ

Protein Analysis

828

Amino Acids

91.86

Weight (kDa)

5.05

Isoelectric Point (pI)

48.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_IPO5	PF25780	127 - 278	1.4e-17	IPO5-like, TPR repeats
Importin_rep_4	PF18808	296 - 387	8.1e-15	Importin repeat
TOR1L1_N	PF24714	373 - 544	9.8e-08	TORTIFOLIA1 N-terminal
HEAT_EZ	PF13513	402 - 456	8.5e-07	HEAT-like repeat
TPR_IMB1	PF25574	491 - 655	1.6e-08	Importin subunit beta-1-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0004551)

Species	Orthologous Gene IDs
malus_domestica	MD11G1005500.v1.1
rosa_chinensis	RchiOBHm_Chr5g0054081 RchiOBHm_Chr5g0054171 RchiOBHm_Chr5g0054201 RchiOBHm_Chr5g0054251
rosa_laevigata	RLG00000034915 RLG00000034916 RLG00000034918 RLG00000034920 RLG00000034921
rosa_multiflora	Rmu_sc0000094.1_g000006 Rmu_sc0000509.1_g000025 Rmu_sc0006889.1_g000007 Rmu_sc0010082.1_g000001 Rmu_sc0023670.1_g000001
rosa_roxburghii	Rroxscaffold_1G00026650 Rroxscaffold_1G00026670
rosa_rugosa	Rorug05G0285300
rosa_samantha	Rh5AG354200 Rh5AG354300 Rh5AG354700 Rh5AG355200 Rh5BG366000 Rh5BG366100 Rh5BG367300 Rh5CG390800 Rh5DG055000 Rh5DG380900 Rh5DG381500
rosa_wichuraiana	Rw5G033390 Rw5G033410 Rw5G033590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	714
Acc36I	ACCTGC	1 cut(s)	1398
AccI	GTMKAC	3 cut(s)	11, 991, 1370
AccII	CGCG	3 cut(s)	366, 551, 644
AccIII	TCCGGA	2 cut(s)	273, 1636
AclI	AACGTT	1 cut(s)	2171
AclWI	GGATC	5 cut(s)	686, 1031, 1831, 2159, 2483
AcoI	YGGCCR	6 cut(s)	33, 458, 804, 909, 933, 1360
AcsI	RAATTY	2 cut(s)	1493, 1772
AcuI	CTGAAG	6 cut(s)	500, 708, 891, 1464, 1928, 2150
AcyI	GRCGYC	1 cut(s)	711
AfaI	GTAC	1 cut(s)	1563
AfiI	CCNNNNNNNGG	5 cut(s)	144, 618, 1682, 1919, 2459
AgeI	ACCGGT	1 cut(s)	290
AhlI	ACTAGT	1 cut(s)	2069
AjnI	CCWGG	2 cut(s)	225, 1743
AleI	CACNNNNGTG	1 cut(s)	1201
Alw21I	GWGCWC	6 cut(s)	162, 263, 420, 468, 611, 955
Alw26I	GTCTC	2 cut(s)	235, 1052
Alw44I	GTGCAC	1 cut(s)	951
AlwI	GGATC	5 cut(s)	686, 1031, 1831, 2159, 2483
AlwNI	CAGNNNCTG	1 cut(s)	28
Ama87I	CYCGRG	2 cut(s)	249, 1199
Aor13HI	TCCGGA	2 cut(s)	273, 1636
ApaLI	GTGCAC	1 cut(s)	951
ApeKI	GCWGC	4 cut(s)	859, 1193, 1220, 1475
ApoI	RAATTY	2 cut(s)	1493, 1772
ArsI	GACNNNNNNTTYG	2 cut(s)	1516, 1548
AseI	ATTAAT	1 cut(s)	1350
AsiGI	ACCGGT	1 cut(s)	290
AspLEI	GCGC	2 cut(s)	505, 1877
AspS9I	GGNCC	8 cut(s)	44, 147, 810, 1206, 1381, 1639, 1719, 2420
AsuC2I	CCSGG	3 cut(s)	808, 851, 1712
AsuHPI	GGTGA	4 cut(s)	280, 305, 1103, 2474
AsuII	TTCGAA	1 cut(s)	389
AvaI	CYCGRG	2 cut(s)	249, 1199
AvaII	GGWCC	6 cut(s)	44, 147, 810, 1639, 1719, 2420
BaeGI	GKGCMC	1 cut(s)	955
BalI	TGGCCA	2 cut(s)	911, 1362
BanII	GRGCYC	5 cut(s)	54, 162, 420, 468, 611
BauI	CACGAG	1 cut(s)	191
BbsI	GAAGAC	3 cut(s)	157, 313, 583
Bbv12I	GWGCWC	6 cut(s)	162, 263, 420, 468, 611, 955
BbvCI	CCTCAGC	1 cut(s)	1248
BbvI	GCAGC	4 cut(s)	846, 1180, 1232, 1462
BccI	CCATC	4 cut(s)	404, 733, 822, 1009
BceAI	ACGGC	1 cut(s)	392
BcgI	CGANNNNNNTGC	6 cut(s)	240, 274, 2144, 2178, 2224, 2258
BciT130I	CCWGG	2 cut(s)	227, 1745
BcnI	CCSGG	3 cut(s)	808, 851, 1712
BcoDI	GTCTC	2 cut(s)	235, 1052
BcuI	ACTAGT	1 cut(s)	2069
BfaI	CTAG	5 cut(s)	1512, 1838, 1895, 2070, 2454
BfmI	CTRYAG	3 cut(s)	860, 1865, 2262
BfuAI	ACCTGC	1 cut(s)	1398
BlpI	GCTNAGC	2 cut(s)	216, 827
Bme1390I	CCNGG	5 cut(s)	227, 808, 851, 1712, 1745
Bme18I	GGWCC	6 cut(s)	44, 147, 810, 1639, 1719, 2420
BmeT110I	CYCGRG	2 cut(s)	249, 1199
BmgT120I	GGNCC	8 cut(s)	44, 147, 810, 1206, 1381, 1639, 1719, 2420
BmiI	GGNNCC	3 cut(s)	811, 812, 1383
BmrFI	CCNGG	5 cut(s)	227, 808, 851, 1712, 1745
BmsI	GCATC	8 cut(s)	28, 490, 1222, 1625, 1687, 1774, 2354, 2380
BpiI	GAAGAC	3 cut(s)	157, 313, 583
BplI	GAGNNNNNCTC	4 cut(s)	407, 439, 1241, 1273
BpmI	CTGGAG	1 cut(s)	209
Bpu10I	CCTNAGC	2 cut(s)	48, 1248
Bpu1102I	GCTNAGC	2 cut(s)	216, 827
Bpu14I	TTCGAA	1 cut(s)	389
BpuEI	CTTGAG	1 cut(s)	625
BpuMI	CCSGG	3 cut(s)	808, 851, 1712
Bsa29I	ATCGAT	2 cut(s)	383, 2154
BsaBI	GATNNNNATC	1 cut(s)	2060
BsaHI	GRCGYC	1 cut(s)	711
BsaI	GGTCTC	1 cut(s)	235
BsaJI	CCNNGG	8 cut(s)	150, 936, 956, 1077, 1137, 1728, 2147, 2281
BsaWI	WCCGGW	3 cut(s)	273, 290, 1636
BsaXI	ACNNNNNCTCC	4 cut(s)	74, 104, 116, 146
Bsc4I	CCNNNNNNNGG	5 cut(s)	144, 618, 1682, 1919, 2459
Bse118I	RCCGGY	1 cut(s)	290
Bse1I	ACTGG	3 cut(s)	33, 1363, 1470
Bse3DI	GCAATG	4 cut(s)	1071, 1131, 1302, 2235
Bse8I	GATNNNNATC	1 cut(s)	2060
BseAI	TCCGGA	2 cut(s)	273, 1636
BseBI	CCWGG	2 cut(s)	227, 1745
BseCI	ATCGAT	2 cut(s)	383, 2154
BseDI	CCNNGG	8 cut(s)	150, 936, 956, 1077, 1137, 1728, 2147, 2281
BseJI	GATNNNNATC	1 cut(s)	2060
BseLI	CCNNNNNNNGG	5 cut(s)	144, 618, 1682, 1919, 2459
BseMI	GCAATG	4 cut(s)	1071, 1131, 1302, 2235
BseMII	CTCAG	8 cut(s)	6, 39, 230, 841, 1047, 1239, 1251, 1670
BseNI	ACTGG	3 cut(s)	33, 1363, 1470
BseRI	GAGGAG	6 cut(s)	82, 91, 124, 260, 601, 982
BseSI	GKGCMC	1 cut(s)	955
BseXI	GCAGC	4 cut(s)	846, 1180, 1232, 1462
BsgI	GTGCAG	2 cut(s)	1179, 1407
Bsh1236I	CGCG	3 cut(s)	366, 551, 644
Bsh1285I	CGRYCG	1 cut(s)	2158
BshTI	ACCGGT	1 cut(s)	290
BshVI	ATCGAT	2 cut(s)	383, 2154
BsiEI	CGRYCG	1 cut(s)	2158
BsiHKAI	GWGCWC	6 cut(s)	162, 263, 420, 468, 611, 955
BsiHKCI	CYCGRG	2 cut(s)	249, 1199
BsiSI	CCGG	8 cut(s)	274, 291, 461, 807, 850, 942, 1637, 1712
BslFI	GGGAC	2 cut(s)	823, 910
BslI	CCNNNNNNNGG	5 cut(s)	144, 618, 1682, 1919, 2459
BsmAI	GTCTC	2 cut(s)	235, 1052
BsmFI	GGGAC	2 cut(s)	823, 910
BsmI	GAATGC	3 cut(s)	527, 1435, 2408
Bso31I	GGTCTC	1 cut(s)	235
BsoBI	CYCGRG	2 cut(s)	249, 1199
Bsp119I	TTCGAA	1 cut(s)	389
Bsp1286I	GDGCHC	7 cut(s)	54, 162, 263, 420, 468, 611, 955
Bsp13I	TCCGGA	2 cut(s)	273, 1636
Bsp1720I	GCTNAGC	2 cut(s)	216, 827
Bsp19I	CCATGG	2 cut(s)	956, 2147
BspCNI	CTCAG	8 cut(s)	7, 40, 229, 840, 1048, 1240, 1252, 1671
BspDI	ATCGAT	2 cut(s)	383, 2154
BspEI	TCCGGA	2 cut(s)	273, 1636
BspFNI	CGCG	3 cut(s)	366, 551, 644
BspHI	TCATGA	1 cut(s)	1668
BspLI	GGNNCC	3 cut(s)	811, 812, 1383
BspMAI	CTGCAG	1 cut(s)	864
BspMI	ACCTGC	1 cut(s)	1398
BspPI	GGATC	5 cut(s)	686, 1031, 1831, 2159, 2483
BspT104I	TTCGAA	1 cut(s)	389
BspTNI	GGTCTC	1 cut(s)	235
BsrDI	GCAATG	4 cut(s)	1071, 1131, 1302, 2235
BsrFI	RCCGGY	1 cut(s)	290
BsrI	ACTGG	3 cut(s)	33, 1363, 1470
BssAI	RCCGGY	1 cut(s)	290
BssECI	CCNNGG	8 cut(s)	150, 936, 956, 1077, 1137, 1728, 2147, 2281
BssNI	GRCGYC	1 cut(s)	711
BssSI	CACGAG	1 cut(s)	191
BssT1I	CCWWGG	5 cut(s)	956, 1077, 1137, 2147, 2281
Bst2BI	CACGAG	1 cut(s)	191
Bst2UI	CCWGG	2 cut(s)	227, 1745
Bst4CI	ACNGT	6 cut(s)	73, 589, 736, 1100, 1457, 1975
BstACI	GRCGYC	1 cut(s)	711
BstAPI	GCANNNNNTGC	3 cut(s)	1229, 1430, 1607
BstBI	TTCGAA	1 cut(s)	389
BstDEI	CTNAG	9 cut(s)	15, 48, 216, 827, 1056, 1248, 1260, 1679, 1788
BstDSI	CCRYGG	3 cut(s)	956, 1728, 2147
BstEII	GGTNACC	1 cut(s)	293
BstENI	CCTNNNNNAGG	2 cut(s)	616, 2457
BstFNI	CGCG	3 cut(s)	366, 551, 644
BstHHI	GCGC	2 cut(s)	505, 1877
BstMAI	GTCTC	2 cut(s)	235, 1052
BstMCI	CGRYCG	1 cut(s)	2158
BstNI	CCWGG	2 cut(s)	227, 1745
BstNSI	RCATGY	3 cut(s)	1595, 1864, 2450
BstPI	GGTNACC	1 cut(s)	293
BstSCI	CCNGG	5 cut(s)	225, 806, 849, 1710, 1743
BstSFI	CTRYAG	3 cut(s)	860, 1865, 2262
BstSLI	GKGCMC	1 cut(s)	955
BstUI	CGCG	3 cut(s)	366, 551, 644
BstV1I	GCAGC	4 cut(s)	846, 1180, 1232, 1462
BstV2I	GAAGAC	3 cut(s)	157, 313, 583
BstX2I	RGATCY	2 cut(s)	691, 1823
BstXI	CCANNNNNNTGG	2 cut(s)	1138, 1729
BstYI	RGATCY	2 cut(s)	691, 1823
Bsu15I	ATCGAT	2 cut(s)	383, 2154
BsuTUI	ATCGAT	2 cut(s)	383, 2154
BtgI	CCRYGG	3 cut(s)	956, 1728, 2147
BtsI	GCAGTG	2 cut(s)	1484, 2373
BtsIMutI	CAGTG	4 cut(s)	741, 1424, 1484, 2373
BveI	ACCTGC	1 cut(s)	1398
CaiI	CAGNNNCTG	1 cut(s)	28
CciI	TCATGA	1 cut(s)	1668
CfoI	GCGC	2 cut(s)	505, 1877
Cfr10I	RCCGGY	1 cut(s)	290
Cfr13I	GGNCC	8 cut(s)	44, 147, 810, 1206, 1381, 1639, 1719, 2420
ClaI	ATCGAT	2 cut(s)	383, 2154
CpoI	CGGWCCG	1 cut(s)	147
Csp6I	GTAC	1 cut(s)	1562
CspAI	ACCGGT	1 cut(s)	290
CspI	CGGWCCG	1 cut(s)	147
CviQI	GTAC	1 cut(s)	1562
DdeI	CTNAG	9 cut(s)	15, 48, 216, 827, 1056, 1248, 1260, 1679, 1788
DraI	TTTAAA	1 cut(s)	2397
EaeI	YGGCCR	6 cut(s)	33, 458, 804, 909, 933, 1360
EciI	GGCGGA	1 cut(s)	20
Ecl136II	GAGCTC	4 cut(s)	160, 418, 466, 609
Eco130I	CCWWGG	5 cut(s)	956, 1077, 1137, 2147, 2281
Eco24I	GRGCYC	5 cut(s)	54, 162, 420, 468, 611
Eco31I	GGTCTC	1 cut(s)	235
Eco32I	GATATC	2 cut(s)	1519, 2415
Eco47I	GGWCC	6 cut(s)	44, 147, 810, 1639, 1719, 2420
Eco53kI	GAGCTC	4 cut(s)	160, 418, 466, 609
Eco57I	CTGAAG	6 cut(s)	500, 708, 891, 1464, 1928, 2150
Eco88I	CYCGRG	2 cut(s)	249, 1199
Eco91I	GGTNACC	1 cut(s)	293
EcoICRI	GAGCTC	4 cut(s)	160, 418, 466, 609
EcoNI	CCTNNNNNAGG	2 cut(s)	616, 2457
EcoO109I	RGGNCCY	1 cut(s)	810
EcoO65I	GGTNACC	1 cut(s)	293
EcoRII	CCWGG	2 cut(s)	225, 1743
EcoRV	GATATC	2 cut(s)	1519, 2415
EcoT14I	CCWWGG	5 cut(s)	956, 1077, 1137, 2147, 2281
EcoT22I	ATGCAT	1 cut(s)	2408
EcoT38I	GRGCYC	5 cut(s)	54, 162, 420, 468, 611
ErhI	CCWWGG	5 cut(s)	956, 1077, 1137, 2147, 2281
FalI	AAGNNNNNCTT	2 cut(s)	1893, 1925
FaqI	GGGAC	2 cut(s)	823, 910
FauI	CCCGC	1 cut(s)	61
FblI	GTMKAC	3 cut(s)	11, 991, 1370
FriOI	GRGCYC	5 cut(s)	54, 162, 420, 468, 611
FspBI	CTAG	5 cut(s)	1512, 1838, 1895, 2070, 2454
GlaI	GCGC	2 cut(s)	504, 1876
GsuI	CTGGAG	1 cut(s)	209
HapII	CCGG	8 cut(s)	274, 291, 461, 807, 850, 942, 1637, 1712
HhaI	GCGC	2 cut(s)	505, 1877
Hin1I	GRCGYC	1 cut(s)	711
Hin6I	GCGC	2 cut(s)	503, 1875
HinP1I	GCGC	2 cut(s)	503, 1875
HindIII	AAGCTT	1 cut(s)	512
HinfI	GANTC	7 cut(s)	8, 194, 309, 1672, 2009, 2061, 2139
HpaII	CCGG	8 cut(s)	274, 291, 461, 807, 850, 942, 1637, 1712
HphI	GGTGA	4 cut(s)	280, 305, 1103, 2474
Hpy166II	GTNNAC	4 cut(s)	12, 953, 992, 1371
Hpy8I	GTNNAC	4 cut(s)	12, 953, 992, 1371
Hpy99I	CGWCG	3 cut(s)	242, 713, 823
HpyAV	CCTTC	8 cut(s)	385, 446, 538, 754, 1919, 2125, 2129, 2336
HpyCH4III	ACNGT	6 cut(s)	73, 589, 736, 1100, 1457, 1975
HpyCH4IV	ACGT	3 cut(s)	711, 821, 2171
HpyF3I	CTNAG	9 cut(s)	15, 48, 216, 827, 1056, 1248, 1260, 1679, 1788
HpySE526I	ACGT	3 cut(s)	711, 821, 2171
Hsp92I	GRCGYC	1 cut(s)	711
HspAI	GCGC	2 cut(s)	503, 1875
KflI	GGGWCCC	1 cut(s)	810
Kpn2I	TCCGGA	2 cut(s)	273, 1636
LmnI	GCTCC	8 cut(s)	266, 415, 463, 530, 614, 1282, 1834, 2170
Lsp1109I	GCAGC	4 cut(s)	846, 1180, 1232, 1462
LweI	GCATC	8 cut(s)	28, 490, 1222, 1625, 1687, 1774, 2354, 2380
MaeI	CTAG	5 cut(s)	1512, 1838, 1895, 2070, 2454
MaeII	ACGT	3 cut(s)	711, 821, 2171
MaeIII	GTNAC	5 cut(s)	293, 1091, 1975, 2089, 2442
MboII	GAAGA	5 cut(s)	157, 318, 583, 701, 1673
MfeI	CAATTG	2 cut(s)	1161, 1553
MflI	RGATCY	2 cut(s)	691, 1823
MhlI	GDGCHC	7 cut(s)	54, 162, 263, 420, 468, 611, 955
MlsI	TGGCCA	2 cut(s)	911, 1362
MluNI	TGGCCA	2 cut(s)	911, 1362
MlyI	GAGTC	3 cut(s)	17, 203, 303
MmeI	TCCRAC	7 cut(s)	147, 393, 842, 1674, 1741, 2058, 2192
Mox20I	TGGCCA	2 cut(s)	911, 1362
Mph1103I	ATGCAT	1 cut(s)	2408
MroI	TCCGGA	2 cut(s)	273, 1636
MscI	TGGCCA	2 cut(s)	911, 1362
MseI	TTAA	6 cut(s)	86, 633, 1007, 1350, 1959, 2396
MslI	CAYNNNNRTG	3 cut(s)	1007, 1201, 1802
Msp20I	TGGCCA	2 cut(s)	911, 1362
MspA1I	CMGCKG	1 cut(s)	1055
MspI	CCGG	8 cut(s)	274, 291, 461, 807, 850, 942, 1637, 1712
MspR9I	CCNGG	5 cut(s)	227, 808, 851, 1712, 1745
MunI	CAATTG	2 cut(s)	1161, 1553
Mva1269I	GAATGC	3 cut(s)	527, 1435, 2408
MvaI	CCWGG	2 cut(s)	227, 1745
MvnI	CGCG	3 cut(s)	366, 551, 644
NciI	CCSGG	3 cut(s)	808, 851, 1712
NcoI	CCATGG	2 cut(s)	956, 2147
NlaIV	GGNNCC	3 cut(s)	811, 812, 1383
NmeAIII	GCCGAG	2 cut(s)	181, 961
NmuCI	GTSAC	3 cut(s)	293, 1091, 2442
NsiI	ATGCAT	1 cut(s)	2408
NspI	RCATGY	3 cut(s)	1595, 1864, 2450
NspV	TTCGAA	1 cut(s)	389
OliI	CACNNNNGTG	1 cut(s)	1201
PaeI	GCATGC	1 cut(s)	1864
PagI	TCATGA	1 cut(s)	1668
PctI	GAATGC	3 cut(s)	527, 1435, 2408
PfeI	GAWTC	4 cut(s)	1672, 2009, 2061, 2139
PinAI	ACCGGT	1 cut(s)	290
Ple19I	CGATCG	1 cut(s)	2158
PleI	GAGTC	3 cut(s)	16, 202, 303
PpsI	GAGTC	3 cut(s)	16, 202, 303
PpuMI	RGGWCCY	1 cut(s)	810
PshBI	ATTAAT	1 cut(s)	1350
Psp124BI	GAGCTC	4 cut(s)	162, 420, 468, 611
Psp1406I	AACGTT	1 cut(s)	2171
Psp5II	RGGWCCY	1 cut(s)	810
Psp6I	CCWGG	2 cut(s)	225, 1743
PspEI	GGTNACC	1 cut(s)	293
PspGI	CCWGG	2 cut(s)	225, 1743
PspN4I	GGNNCC	3 cut(s)	811, 812, 1383
PspPI	GGNCC	8 cut(s)	44, 147, 810, 1206, 1381, 1639, 1719, 2420
PspPPI	RGGWCCY	1 cut(s)	810
PstI	CTGCAG	1 cut(s)	864
PstNI	CAGNNNCTG	1 cut(s)	28
PsuI	RGATCY	2 cut(s)	691, 1823
PvuI	CGATCG	1 cut(s)	2158
PvuII	CAGCTG	1 cut(s)	1055
RsaI	GTAC	1 cut(s)	1563
RsaNI	GTAC	1 cut(s)	1562
RseI	CAYNNNNRTG	3 cut(s)	1007, 1201, 1802
Rsr2I	CGGWCCG	1 cut(s)	147
RsrII	CGGWCCG	1 cut(s)	147
SacI	GAGCTC	4 cut(s)	162, 420, 468, 611
SaqAI	TTAA	6 cut(s)	86, 633, 1007, 1350, 1959, 2396
Sau96I	GGNCC	8 cut(s)	44, 147, 810, 1206, 1381, 1639, 1719, 2420
SchI	GAGTC	3 cut(s)	17, 203, 303
ScrFI	CCNGG	5 cut(s)	227, 808, 851, 1712, 1745
SduI	GDGCHC	7 cut(s)	54, 162, 263, 420, 468, 611, 955
SfaNI	GCATC	8 cut(s)	28, 490, 1222, 1625, 1687, 1774, 2354, 2380
SfcI	CTRYAG	3 cut(s)	860, 1865, 2262
SfuI	TTCGAA	1 cut(s)	389
SgrAI	CRCCGGYG	1 cut(s)	290
SinI	GGWCC	6 cut(s)	44, 147, 810, 1639, 1719, 2420
SmiMI	CAYNNNNRTG	3 cut(s)	1007, 1201, 1802
SmlI	CTYRAG	1 cut(s)	604
SmoI	CTYRAG	1 cut(s)	604
SpeI	ACTAGT	1 cut(s)	2069
SphI	GCATGC	1 cut(s)	1864
SspI	AATATT	1 cut(s)	2385
SspMI	CTAG	5 cut(s)	1512, 1838, 1895, 2070, 2454
SstI	GAGCTC	4 cut(s)	162, 420, 468, 611
StyD4I	CCNGG	5 cut(s)	225, 806, 849, 1710, 1743
StyI	CCWWGG	5 cut(s)	956, 1077, 1137, 2147, 2281
TaaI	ACNGT	6 cut(s)	73, 589, 736, 1100, 1457, 1975
TaiI	ACGT	3 cut(s)	714, 824, 2174
TaqI	TCGA	6 cut(s)	383, 389, 540, 2154, 2428, 2458
TaqII	GACCGA	1 cut(s)	32
TatI	WGTACW	1 cut(s)	1561
TauI	GCSGC	6 cut(s)	35, 59, 450, 508, 644, 834
TfiI	GAWTC	4 cut(s)	1672, 2009, 2061, 2139
Tru1I	TTAA	6 cut(s)	86, 633, 1007, 1350, 1959, 2396
Tru9I	TTAA	6 cut(s)	86, 633, 1007, 1350, 1959, 2396
TscAI	CASTG	4 cut(s)	741, 1431, 1491, 2380
TseFI	GTSAC	3 cut(s)	293, 1091, 2442
TseI	GCWGC	4 cut(s)	859, 1193, 1220, 1475
Tsp45I	GTSAC	3 cut(s)	293, 1091, 2442
TspGWI	ACGGA	1 cut(s)	1717
TspRI	CASTG	4 cut(s)	741, 1431, 1491, 2380
VneI	GTGCAC	1 cut(s)	951
VpaK11BI	GGWCC	6 cut(s)	44, 147, 810, 1639, 1719, 2420
VspI	ATTAAT	1 cut(s)	1350
XagI	CCTNNNNNAGG	2 cut(s)	616, 2457
XapI	RAATTY	2 cut(s)	1493, 1772
XceI	RCATGY	3 cut(s)	1595, 1864, 2450
XcmI	CCANNNNNNNNNTGG	1 cut(s)	883
XmiI	GTMKAC	3 cut(s)	11, 991, 1370
XspI	CTAG	5 cut(s)	1512, 1838, 1895, 2070, 2454
ZraI	GACGTC	1 cut(s)	712
Zsp2I	ATGCAT	1 cut(s)	2408

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.