Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0004551

Physical Location & Seq

Genomic Coordinates Reverse (-)

57068417 .. 57075574

Gene Structure 7158 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000034921

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2322 bp

ATGGCGGAGTCCACTGAGCATCAACAACTGGCGGCCATTCTCGGTCCTGAGCCCGCCGCCACTTTCCAAACCGTTATCTCCCACTTAATTTCCTCCCCCTCCTCTTTGCAAACCCTAATCTCTGACCTCACTTCCTCCGAGAAGCGGTCCGAGGCCGAGCTCGTCTTCAACATCTGCAAGCAGGCCGATCCCGAGTCTCTTTCCCTCAAACTCGCTCAGCTTCTCCAGGTCTCTCCCGACGAGAAGACCCAAGCCATGTGCTCCATTCTCCTCCGGAAGCAACTACTCACCCGAGACCTGTGGCGAAGACTCTCTCGTAACACCAAATCCACCCTCAAACATACTCTATTATCTTGTATTCAACGCGAGGACACCCCAACATCGATTTCGAAGGCCATCTGCTACGCCGTGTCTGAGCTCGCCTCCGCAGTGTTGTTCCCCGAAGGCTGGCCGGAGCTCTTGCCCTTTCAGTTCCAATCCACCTCCTACGATGCGCCGCCGAAGCTTCAGGAATGCTCCTTCTTGATTTTCGATCAGCTCGCGTACTTCTTTAGCCCGTTTATAGACGAATTACACACCGTCTTCTTATACTGCTTGAGCTCCTCCACAAGGTCAAATGAAGTTAAGATAGCCGCGTTTAGTGCGGTGGTCAGTTTCATTCGGTGTTTGTCCAGCTCTGACGATCCGAATATGTTTCACGACGTCTTGATAGAAATGATGAGAACAGTGATGGGGGAATTGAACAAAGGGAAGGAGATCATGGCACAGGAGGCGATCAAGTTGTTCATTGAATTGGCTGGGACCCATCCGACGTTGCTCAGCCTCAGCGGGCAGATCATCCGGGCTATGCTGCAGATTGCTGAAGCCATAGAAGTCTTGGAACAAGGGACGAGGCACTTGGCCATTGAGTTTCTGATTACTCTGGCCGAGGCCGGACAGCGTGCACCATGGATAATGAGGAGGTGTCGGGAGTTTGTTGGTCTACTTTTTCCCATATTAATGAGGATGGTTTCAAATGTCAAGGATGATCCCTCATGGCATACAGCTGAGACTGACGATGACATTGCCTTGGGTGTGAGTGGTGATTACTGTGTTGGGCAGGAGTGTTTGCATAGGCTTGCCATTGCCTTGGGTGGCAATAACATTGTTCCAATTGCGTTAGAACACTTTACTTCCCATTTTGCTGCACCCGAGTGGCCCAAGCATCATGCAGCATTGATTGCCCTTGCTCAAATCGCTGAGGGTTGCTCTGACGTAATGATAAAAGATTTGGAGCAAGTGGTGGCAATGGTTTTGAACTCCCTTGAACATCCACATATTCGGTTAAGGTGGTCAGCTATTAATGCAGTGTCTTCTGCACTGAATGCCACTATATATTTTTCTGCACCGAATGCCACTATATATGGTTTGCAGAACCGTCGTCTGCTGGCACATGCTGCTTCAGCACTGCGAAAATTCATTGAGAAATGCGCTAGGGATATCTTGACACCTTATTTGGATGAAGTGGTTTCCCAATTGGTTGCACTTTTGCAGAGTGAGATACAAATGGTGCAAGTAGAAGCATTGACTGCTTTGGCATCAGTTGTTGTTTCATTTCCGGAGCAATTCCAAAAATACTATGAAGTTGTCATGATTCCCCTGAGATTTATGTTGTTGGATGCCACTAATAACCCGGTTTGGTCCAAGTCCGTGGCGTGTATTAACTTGGTTGGTATGGCTGTTGGAAAGGAGGATTCTAAGAAAGTCATGGAAGTGCTGATGTCTTTGCAAGGATCTCAAATGGAGCCAGATGACCTAACTACAAGTTGCATGCTACAGATATGCGCTGTCCTCTGCAAGTGTCTAGGACAAGATTTCCTTCCGTACATGAGTGCTGTTATGCCTCCTTTGCTTCAGTTTGTTCAAATTAAGCCTGTTGAAACTGTTACCTCTGCAAATGACAATTGTGATATGGATGAATCTGATGATGAAAGTATGGAAACAATTACTCGTGGAGATAAAAGGATTAGAATCAAGACTAGTGTATTGGAGCAAAAAGTTACAGCTTGCAATTTGCTGTGTTGCTATGCTGATGAACTGAAGGAAGGATTCTTTCCATGGATCGATCAGGAAGATGATTCTGAAATCTGCATAAATATATTGGATGCTATAGAAGAATTTAAACAGATCTTTGCATCCACTCTAGATGAAAGCCATGTCAAATCCATCATGGAGATAAAGCAGGTGAGTACTTCTTACTTGAAAGAAAGAAGGAGAGCAGAAGGGACCTCAGCATATGATTTTGATGATGAGGAAAGGAAGGCGATGAAACTGGAAAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000060 GO:0001101 GO:0003674 GO:0005048 GO:0005215 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008139 GO:0008150 GO:0008565 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0017038 GO:0031090 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032386 GO:0032388 GO:0032879 GO:0032880 GO:0033036 GO:0033157 GO:0033218 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0042221 GO:0042277 GO:0042306 GO:0042307 GO:0042886 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046822 GO:0046824 GO:0046907 GO:0048518 GO:0050789 GO:0050896 GO:0051049 GO:0051050 GO:0051169 GO:0051170 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0060341 GO:0065007 GO:0070013 GO:0070201 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071702 GO:0071705 GO:0072594 GO:0090087 GO:0090316 GO:1900180 GO:1900182 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1903827 GO:1903829 GO:1904589 GO:1904591 GO:1904951

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HEAT HEAT_2 HEAT_EZ

Protein Analysis

774

Amino Acids

86.43

Weight (kDa)

4.97

Isoelectric Point (pI)

53.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_IPO5	PF25780	128 - 274	8.5e-16	IPO5-like, TPR repeats
Importin_rep_4	PF18808	293 - 384	8.2e-15	Importin repeat
TPR_IMB1	PF25574	477 - 639	7.2e-06	Importin subunit beta-1-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004551)

Species	Orthologous Gene IDs
malus_domestica	MD11G1005500.v1.1
rosa_chinensis	RchiOBHm_Chr5g0054081 RchiOBHm_Chr5g0054171 RchiOBHm_Chr5g0054201 RchiOBHm_Chr5g0054251
rosa_laevigata	RLG00000034915 RLG00000034916 RLG00000034918 RLG00000034920 RLG00000034921
rosa_multiflora	Rmu_sc0000094.1_g000006 Rmu_sc0000509.1_g000025 Rmu_sc0006889.1_g000007 Rmu_sc0010082.1_g000001 Rmu_sc0023670.1_g000001
rosa_roxburghii	Rroxscaffold_1G00026650 Rroxscaffold_1G00026670
rosa_rugosa	Rorug05G0285300
rosa_samantha	Rh5AG354200 Rh5AG354300 Rh5AG354700 Rh5AG355200 Rh5BG366000 Rh5BG366100 Rh5BG367300 Rh5CG390800 Rh5DG055000 Rh5DG380900 Rh5DG381500
rosa_wichuraiana	Rw5G033390 Rw5G033410 Rw5G033590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2212
AatII	GACGTC	1 cut(s)	705
Acc36I	ACCTGC	1 cut(s)	2212
AccI	GTMKAC	1 cut(s)	982
AccII	CGCG	3 cut(s)	366, 542, 635
AccIII	TCCGGA	2 cut(s)	273, 1597
AclWI	GGATC	5 cut(s)	182, 677, 1022, 1780, 2108
AcoI	YGGCCR	4 cut(s)	33, 449, 900, 924
AcsI	RAATTY	2 cut(s)	1454, 2156
AcuI	CTGAAG	5 cut(s)	491, 882, 1425, 1877, 2099
AcyI	GRCGYC	1 cut(s)	702
AfaI	GTAC	3 cut(s)	545, 1865, 2230
AfiI	CCNNNNNNNGG	3 cut(s)	144, 447, 609
AhlI	ACTAGT	1 cut(s)	2018
AjnI	CCWGG	1 cut(s)	225
AjuI	GAANNNNNNNTTGG	2 cut(s)	370, 402
AleI	CACNNNNGTG	1 cut(s)	1192
Alw21I	GWGCWC	6 cut(s)	162, 263, 420, 459, 602, 946
Alw26I	GTCTC	4 cut(s)	201, 235, 288, 1043
Alw44I	GTGCAC	1 cut(s)	942
AlwI	GGATC	5 cut(s)	182, 677, 1022, 1780, 2108
Ama87I	CYCGRG	3 cut(s)	191, 291, 1190
Aor13HI	TCCGGA	2 cut(s)	273, 1597
AoxI	GGCC	9 cut(s)	33, 153, 183, 393, 449, 900, 924, 930, 1196
ApaLI	GTGCAC	1 cut(s)	942
ApeKI	GCWGC	4 cut(s)	850, 1184, 1211, 1436
ApoI	RAATTY	2 cut(s)	1454, 2156
ArsI	GACNNNNNNTTYG	2 cut(s)	1477, 1509
AseI	ATTAAT	2 cut(s)	998, 1341
AspLEI	GCGC	3 cut(s)	496, 1472, 1826
AspS9I	GGNCC	6 cut(s)	44, 147, 801, 1197, 1680, 2265
AsuC2I	CCSGG	2 cut(s)	842, 1673
AsuHPI	GGTGA	3 cut(s)	280, 1094, 2236
AsuII	TTCGAA	1 cut(s)	389
AvaI	CYCGRG	3 cut(s)	191, 291, 1190
AvaII	GGWCC	5 cut(s)	44, 147, 801, 1680, 2265
BaeGI	GKGCMC	1 cut(s)	946
BalI	TGGCCA	1 cut(s)	902
BanII	GRGCYC	5 cut(s)	54, 162, 420, 459, 602
BauI	CACGAG	1 cut(s)	1989
BbsI	GAAGAC	5 cut(s)	157, 251, 313, 574, 1344
Bbv12I	GWGCWC	6 cut(s)	162, 263, 420, 459, 602, 946
BbvCI	CCTCAGC	3 cut(s)	824, 1239, 2269
BbvI	GCAGC	4 cut(s)	837, 1171, 1223, 1423
BccI	CCATC	5 cut(s)	404, 724, 813, 1000, 2213
BceAI	ACGGC	1 cut(s)	392
BcgI	CGANNNNNNTGC	4 cut(s)	1046, 1080, 1400, 1434
BciT130I	CCWGG	1 cut(s)	227
BcnI	CCSGG	2 cut(s)	842, 1673
BcoDI	GTCTC	4 cut(s)	201, 235, 288, 1043
BcuI	ACTAGT	1 cut(s)	2018
BfaI	CTAG	4 cut(s)	1473, 1844, 2019, 2183
BfmI	CTRYAG	3 cut(s)	851, 1814, 2148
BfuAI	ACCTGC	1 cut(s)	2212
BglII	AGATCT	1 cut(s)	2166
BisI	GCNGC	8 cut(s)	33, 57, 497, 633, 851, 1185, 1212, 1437
BlpI	GCTNAGC	2 cut(s)	216, 818
BlsI	GCNGC	8 cut(s)	34, 58, 498, 634, 852, 1186, 1213, 1438
BmcAI	AGTACT	1 cut(s)	2230
Bme1390I	CCNGG	3 cut(s)	227, 842, 1673
Bme18I	GGWCC	5 cut(s)	44, 147, 801, 1680, 2265
BmeT110I	CYCGRG	3 cut(s)	191, 291, 1190
BmgT120I	GGNCC	6 cut(s)	44, 147, 801, 1197, 1680, 2265
BmiI	GGNNCC	4 cut(s)	802, 803, 1785, 2266
BmrFI	CCNGG	3 cut(s)	227, 842, 1673
BmsI	GCATC	7 cut(s)	28, 481, 1213, 1586, 1648, 2134, 2183
BpiI	GAAGAC	5 cut(s)	157, 251, 313, 574, 1344
BplI	GAGNNNNNCTC	4 cut(s)	407, 439, 1232, 1264
BpmI	CTGGAG	1 cut(s)	209
Bpu10I	CCTNAGC	4 cut(s)	48, 824, 1239, 2269
Bpu1102I	GCTNAGC	2 cut(s)	216, 818
Bpu14I	TTCGAA	1 cut(s)	389
BpuEI	CTTGAG	1 cut(s)	616
BpuMI	CCSGG	2 cut(s)	842, 1673
Bsa29I	ATCGAT	2 cut(s)	383, 2103
BsaBI	GATNNNNATC	1 cut(s)	2009
BsaHI	GRCGYC	1 cut(s)	702
BsaI	GGTCTC	2 cut(s)	235, 288
BsaJI	CCNNGG	7 cut(s)	150, 927, 947, 1068, 1128, 1689, 2096
BsaWI	WCCGGW	2 cut(s)	273, 1597
Bsc4I	CCNNNNNNNGG	3 cut(s)	144, 447, 609
Bse1I	ACTGG	2 cut(s)	33, 2316
Bse3DI	GCAATG	3 cut(s)	1062, 1122, 1293
Bse8I	GATNNNNATC	1 cut(s)	2009
BseAI	TCCGGA	2 cut(s)	273, 1597
BseBI	CCWGG	1 cut(s)	227
BseCI	ATCGAT	2 cut(s)	383, 2103
BseDI	CCNNGG	7 cut(s)	150, 927, 947, 1068, 1128, 1689, 2096
BseJI	GATNNNNATC	1 cut(s)	2009
BseLI	CCNNNNNNNGG	3 cut(s)	144, 447, 609
BseMI	GCAATG	3 cut(s)	1062, 1122, 1293
BseNI	ACTGG	2 cut(s)	33, 2316
BseRI	GAGGAG	4 cut(s)	91, 260, 592, 973
BseSI	GKGCMC	1 cut(s)	946
BseXI	GCAGC	4 cut(s)	837, 1171, 1223, 1423
BseYI	CCCAGC	1 cut(s)	797
BsgI	GTGCAG	3 cut(s)	1170, 1341, 1368
Bsh1236I	CGCG	3 cut(s)	366, 542, 635
BshFI	GGCC	9 cut(s)	35, 155, 185, 395, 451, 902, 926, 932, 1198
BshVI	ATCGAT	2 cut(s)	383, 2103
BsiHKAI	GWGCWC	6 cut(s)	162, 263, 420, 459, 602, 946
BsiHKCI	CYCGRG	3 cut(s)	191, 291, 1190
BsiSI	CCGG	6 cut(s)	274, 452, 841, 933, 1598, 1673
BslFI	GGGAC	3 cut(s)	814, 901, 2278
BslI	CCNNNNNNNGG	3 cut(s)	144, 447, 609
BsmAI	GTCTC	4 cut(s)	201, 235, 288, 1043
BsmFI	GGGAC	3 cut(s)	814, 901, 2278
BsmI	GAATGC	3 cut(s)	518, 1369, 1396
BsnI	GGCC	9 cut(s)	35, 155, 185, 395, 451, 902, 926, 932, 1198
Bso31I	GGTCTC	2 cut(s)	235, 288
BsoBI	CYCGRG	3 cut(s)	191, 291, 1190
Bsp119I	TTCGAA	1 cut(s)	389
Bsp1286I	GDGCHC	7 cut(s)	54, 162, 263, 420, 459, 602, 946
Bsp13I	TCCGGA	2 cut(s)	273, 1597
Bsp1720I	GCTNAGC	2 cut(s)	216, 818
Bsp19I	CCATGG	2 cut(s)	947, 2096
BspANI	GGCC	9 cut(s)	35, 155, 185, 395, 451, 902, 926, 932, 1198
BspDI	ATCGAT	2 cut(s)	383, 2103
BspEI	TCCGGA	2 cut(s)	273, 1597
BspFNI	CGCG	3 cut(s)	366, 542, 635
BspHI	TCATGA	1 cut(s)	1629
BspLI	GGNNCC	4 cut(s)	802, 803, 1785, 2266
BspMAI	CTGCAG	1 cut(s)	855
BspMI	ACCTGC	1 cut(s)	2212
BspPI	GGATC	5 cut(s)	182, 677, 1022, 1780, 2108
BspT104I	TTCGAA	1 cut(s)	389
BspTNI	GGTCTC	2 cut(s)	235, 288
BsrDI	GCAATG	3 cut(s)	1062, 1122, 1293
BsrI	ACTGG	2 cut(s)	33, 2316
BssECI	CCNNGG	7 cut(s)	150, 927, 947, 1068, 1128, 1689, 2096
BssNI	GRCGYC	1 cut(s)	702
BssSI	CACGAG	1 cut(s)	1989
BssT1I	CCWWGG	4 cut(s)	947, 1068, 1128, 2096
Bst2BI	CACGAG	1 cut(s)	1989
Bst2UI	CCWGG	1 cut(s)	227
Bst4CI	ACNGT	6 cut(s)	73, 580, 727, 1091, 1418, 1924
BstACI	GRCGYC	1 cut(s)	702
BstAPI	GCANNNNNTGC	5 cut(s)	1220, 1364, 1391, 1436, 1568
BstBI	TTCGAA	1 cut(s)	389
BstDSI	CCRYGG	3 cut(s)	947, 1689, 2096
BstENI	CCTNNNNNAGG	1 cut(s)	607
BstFNI	CGCG	3 cut(s)	366, 542, 635
BstHHI	GCGC	3 cut(s)	496, 1472, 1826
BstMAI	GTCTC	4 cut(s)	201, 235, 288, 1043
BstNI	CCWGG	1 cut(s)	227
BstNSI	RCATGY	2 cut(s)	1436, 1813
BstSCI	CCNGG	3 cut(s)	225, 840, 1671
BstSFI	CTRYAG	3 cut(s)	851, 1814, 2148
BstSLI	GKGCMC	1 cut(s)	946
BstUI	CGCG	3 cut(s)	366, 542, 635
BstV1I	GCAGC	4 cut(s)	837, 1171, 1223, 1423
BstV2I	GAAGAC	5 cut(s)	157, 251, 313, 574, 1344
BstX2I	RGATCY	2 cut(s)	1772, 2166
BstXI	CCANNNNNNTGG	2 cut(s)	1129, 1690
BstYI	RGATCY	2 cut(s)	1772, 2166
Bsu15I	ATCGAT	2 cut(s)	383, 2103
BsuRI	GGCC	9 cut(s)	35, 155, 185, 395, 451, 902, 926, 932, 1198
BsuTUI	ATCGAT	2 cut(s)	383, 2103
BtgI	CCRYGG	3 cut(s)	947, 1689, 2096
BtgZI	GCGATG	1 cut(s)	2318
BtsI	GCAGTG	3 cut(s)	435, 1353, 1445
BtsIMutI	CAGTG	6 cut(s)	12, 435, 732, 1353, 1358, 1445
BveI	ACCTGC	1 cut(s)	2212
CciI	TCATGA	1 cut(s)	1629
CfoI	GCGC	3 cut(s)	496, 1472, 1826
Cfr13I	GGNCC	6 cut(s)	44, 147, 801, 1197, 1680, 2265
ClaI	ATCGAT	2 cut(s)	383, 2103
CpoI	CGGWCCG	1 cut(s)	147
Csp6I	GTAC	3 cut(s)	544, 1864, 2229
CspI	CGGWCCG	1 cut(s)	147
CviQI	GTAC	3 cut(s)	544, 1864, 2229
DraI	TTTAAA	1 cut(s)	2161
EaeI	YGGCCR	4 cut(s)	33, 449, 900, 924
EciI	GGCGGA	1 cut(s)	20
Ecl136II	GAGCTC	4 cut(s)	160, 418, 457, 600
Eco130I	CCWWGG	4 cut(s)	947, 1068, 1128, 2096
Eco24I	GRGCYC	5 cut(s)	54, 162, 420, 459, 602
Eco31I	GGTCTC	2 cut(s)	235, 288
Eco32I	GATATC	1 cut(s)	1480
Eco47I	GGWCC	5 cut(s)	44, 147, 801, 1680, 2265
Eco53kI	GAGCTC	4 cut(s)	160, 418, 457, 600
Eco57I	CTGAAG	5 cut(s)	491, 882, 1425, 1877, 2099
Eco88I	CYCGRG	3 cut(s)	191, 291, 1190
EcoICRI	GAGCTC	4 cut(s)	160, 418, 457, 600
EcoNI	CCTNNNNNAGG	1 cut(s)	607
EcoO109I	RGGNCCY	2 cut(s)	801, 2265
EcoRII	CCWGG	1 cut(s)	225
EcoRV	GATATC	1 cut(s)	1480
EcoT14I	CCWWGG	4 cut(s)	947, 1068, 1128, 2096
EcoT38I	GRGCYC	5 cut(s)	54, 162, 420, 459, 602
ErhI	CCWWGG	4 cut(s)	947, 1068, 1128, 2096
FalI	AAGNNNNNCTT	2 cut(s)	1842, 1874
FaqI	GGGAC	3 cut(s)	814, 901, 2278
FauI	CCCGC	2 cut(s)	61, 821
FauNDI	CATATG	1 cut(s)	2275
FblI	GTMKAC	1 cut(s)	982
Fnu4HI	GCNGC	8 cut(s)	33, 57, 497, 633, 851, 1185, 1212, 1437
FriOI	GRGCYC	5 cut(s)	54, 162, 420, 459, 602
Fsp4HI	GCNGC	8 cut(s)	33, 57, 497, 633, 851, 1185, 1212, 1437
FspBI	CTAG	4 cut(s)	1473, 1844, 2019, 2183
GlaI	GCGC	3 cut(s)	495, 1471, 1825
GluI	GCNGC	8 cut(s)	33, 57, 497, 633, 851, 1185, 1212, 1437
GsaI	CCCAGC	1 cut(s)	801
GsuI	CTGGAG	1 cut(s)	209
HaeIII	GGCC	9 cut(s)	35, 155, 185, 395, 451, 902, 926, 932, 1198
HapII	CCGG	6 cut(s)	274, 452, 841, 933, 1598, 1673
HhaI	GCGC	3 cut(s)	496, 1472, 1826
Hin1I	GRCGYC	1 cut(s)	702
Hin6I	GCGC	3 cut(s)	494, 1470, 1824
HinP1I	GCGC	3 cut(s)	494, 1470, 1824
HindIII	AAGCTT	1 cut(s)	503
HinfI	GANTC	9 cut(s)	8, 194, 309, 1633, 1733, 1958, 2010, 2088, 2117
HpaII	CCGG	6 cut(s)	274, 452, 841, 933, 1598, 1673
HphI	GGTGA	3 cut(s)	280, 1094, 2236
Hpy166II	GTNNAC	3 cut(s)	12, 944, 983
Hpy8I	GTNNAC	3 cut(s)	12, 944, 983
Hpy99I	CGWCG	4 cut(s)	242, 704, 814, 1422
HpyCH4III	ACNGT	6 cut(s)	73, 580, 727, 1091, 1418, 1924
HpyCH4IV	ACGT	3 cut(s)	702, 812, 1254
HpySE526I	ACGT	3 cut(s)	702, 812, 1254
Hsp92I	GRCGYC	1 cut(s)	702
HspAI	GCGC	3 cut(s)	494, 1470, 1824
KflI	GGGWCCC	1 cut(s)	801
Kpn2I	TCCGGA	2 cut(s)	273, 1597
LmnI	GCTCC	8 cut(s)	266, 454, 521, 605, 1273, 1600, 1783, 2029
Lsp1109I	GCAGC	4 cut(s)	837, 1171, 1223, 1423
LweI	GCATC	7 cut(s)	28, 481, 1213, 1586, 1648, 2134, 2183
MaeI	CTAG	4 cut(s)	1473, 1844, 2019, 2183
MaeII	ACGT	3 cut(s)	702, 812, 1254
MaeIII	GTNAC	3 cut(s)	317, 1924, 2038
MboII	GAAGA	7 cut(s)	157, 256, 318, 574, 1344, 2123, 2165
MfeI	CAATTG	3 cut(s)	1152, 1514, 1942
MflI	RGATCY	2 cut(s)	1772, 2166
MhlI	GDGCHC	7 cut(s)	54, 162, 263, 420, 459, 602, 946
MlsI	TGGCCA	1 cut(s)	902
MluNI	TGGCCA	1 cut(s)	902
MlyI	GAGTC	3 cut(s)	17, 203, 303
MmeI	TCCRAC	3 cut(s)	833, 1635, 1702
Mox20I	TGGCCA	1 cut(s)	902
MroI	TCCGGA	2 cut(s)	273, 1597
MscI	TGGCCA	1 cut(s)	902
MseI	TTAA	9 cut(s)	86, 624, 998, 1325, 1341, 1701, 1908, 2160, 2320
MslI	CAYNNNNRTG	3 cut(s)	998, 1192, 1751
Msp20I	TGGCCA	1 cut(s)	902
MspA1I	CMGCKG	2 cut(s)	828, 1046
MspI	CCGG	6 cut(s)	274, 452, 841, 933, 1598, 1673
MspR9I	CCNGG	3 cut(s)	227, 842, 1673
MunI	CAATTG	3 cut(s)	1152, 1514, 1942
Mva1269I	GAATGC	3 cut(s)	518, 1369, 1396
MvaI	CCWGG	1 cut(s)	227
MvnI	CGCG	3 cut(s)	366, 542, 635
NciI	CCSGG	2 cut(s)	842, 1673
NcoI	CCATGG	2 cut(s)	947, 2096
NdeI	CATATG	1 cut(s)	2275
NlaIV	GGNNCC	4 cut(s)	802, 803, 1785, 2266
NmeAIII	GCCGAG	2 cut(s)	181, 952
NspI	RCATGY	2 cut(s)	1436, 1813
NspV	TTCGAA	1 cut(s)	389
OliI	CACNNNNGTG	1 cut(s)	1192
PaeI	GCATGC	1 cut(s)	1813
PagI	TCATGA	1 cut(s)	1629
PaqCI	CACCTGC	1 cut(s)	2212
PctI	GAATGC	3 cut(s)	518, 1369, 1396
PfeI	GAWTC	6 cut(s)	1633, 1733, 1958, 2010, 2088, 2117
PkrI	GCNGC	8 cut(s)	34, 58, 498, 634, 852, 1186, 1213, 1438
PleI	GAGTC	3 cut(s)	16, 202, 303
PpsI	GAGTC	3 cut(s)	16, 202, 303
PpuMI	RGGWCCY	2 cut(s)	801, 2265
PshBI	ATTAAT	2 cut(s)	998, 1341
Psp124BI	GAGCTC	4 cut(s)	162, 420, 459, 602
Psp5II	RGGWCCY	2 cut(s)	801, 2265
Psp6I	CCWGG	1 cut(s)	225
PspFI	CCCAGC	1 cut(s)	797
PspGI	CCWGG	1 cut(s)	225
PspN4I	GGNNCC	4 cut(s)	802, 803, 1785, 2266
PspPI	GGNCC	6 cut(s)	44, 147, 801, 1197, 1680, 2265
PspPPI	RGGWCCY	2 cut(s)	801, 2265
PstI	CTGCAG	1 cut(s)	855
PsuI	RGATCY	2 cut(s)	1772, 2166
PvuII	CAGCTG	1 cut(s)	1046
RsaI	GTAC	3 cut(s)	545, 1865, 2230
RsaNI	GTAC	3 cut(s)	544, 1864, 2229
RseI	CAYNNNNRTG	3 cut(s)	998, 1192, 1751
Rsr2I	CGGWCCG	1 cut(s)	147
RsrII	CGGWCCG	1 cut(s)	147
SacI	GAGCTC	4 cut(s)	162, 420, 459, 602
SaqAI	TTAA	9 cut(s)	86, 624, 998, 1325, 1341, 1701, 1908, 2160, 2320
SatI	GCNGC	8 cut(s)	33, 57, 497, 633, 851, 1185, 1212, 1437
Sau96I	GGNCC	6 cut(s)	44, 147, 801, 1197, 1680, 2265
ScaI	AGTACT	1 cut(s)	2230
SchI	GAGTC	3 cut(s)	17, 203, 303
ScrFI	CCNGG	3 cut(s)	227, 842, 1673
SduI	GDGCHC	7 cut(s)	54, 162, 263, 420, 459, 602, 946
SfaNI	GCATC	7 cut(s)	28, 481, 1213, 1586, 1648, 2134, 2183
SfcI	CTRYAG	3 cut(s)	851, 1814, 2148
SfuI	TTCGAA	1 cut(s)	389
SinI	GGWCC	5 cut(s)	44, 147, 801, 1680, 2265
SmiMI	CAYNNNNRTG	3 cut(s)	998, 1192, 1751
SmlI	CTYRAG	1 cut(s)	595
SmoI	CTYRAG	1 cut(s)	595
SpeI	ACTAGT	1 cut(s)	2018
SphI	GCATGC	1 cut(s)	1813
SspMI	CTAG	4 cut(s)	1473, 1844, 2019, 2183
SstI	GAGCTC	4 cut(s)	162, 420, 459, 602
StyD4I	CCNGG	3 cut(s)	225, 840, 1671
StyI	CCWWGG	4 cut(s)	947, 1068, 1128, 2096
TaaI	ACNGT	6 cut(s)	73, 580, 727, 1091, 1418, 1924
TaiI	ACGT	3 cut(s)	705, 815, 1257
TaqI	TCGA	4 cut(s)	383, 389, 531, 2103
TaqII	GACCGA	1 cut(s)	32
TatI	WGTACW	1 cut(s)	2228
TauI	GCSGC	4 cut(s)	35, 59, 499, 635
TfiI	GAWTC	6 cut(s)	1633, 1733, 1958, 2010, 2088, 2117
Tru1I	TTAA	9 cut(s)	86, 624, 998, 1325, 1341, 1701, 1908, 2160, 2320
Tru9I	TTAA	9 cut(s)	86, 624, 998, 1325, 1341, 1701, 1908, 2160, 2320
TscAI	CASTG	6 cut(s)	19, 435, 732, 1353, 1365, 1452
TseI	GCWGC	4 cut(s)	850, 1184, 1211, 1436
TspGWI	ACGGA	2 cut(s)	1678, 1851
TspRI	CASTG	6 cut(s)	19, 435, 732, 1353, 1365, 1452
VneI	GTGCAC	1 cut(s)	942
VpaK11BI	GGWCC	5 cut(s)	44, 147, 801, 1680, 2265
VspI	ATTAAT	2 cut(s)	998, 1341
XagI	CCTNNNNNAGG	1 cut(s)	607
XapI	RAATTY	2 cut(s)	1454, 2156
XbaI	TCTAGA	1 cut(s)	2182
XceI	RCATGY	2 cut(s)	1436, 1813
XcmI	CCANNNNNNNNNTGG	1 cut(s)	874
XmiI	GTMKAC	1 cut(s)	982
XspI	CTAG	4 cut(s)	1473, 1844, 2019, 2183
ZraI	GACGTC	1 cut(s)	703
ZrmI	AGTACT	1 cut(s)	2230

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.