Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0004551

Physical Location & Seq

Genomic Coordinates Forward (+)

56640377 .. 56646006

Gene Structure 5630 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G033390.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2529 bp

ATGGCCGAGTCGACTCAGCTCCAGCTTCAACTTGAGCAAGTCGCGACGATTCTTGGCCCCGACCCGCTCGCCTCTTTCAAAACCCTAATCTCCTACCTCCTCTGCCCCTCCTATTTTGAAAGCCTAATCTCCTCCTCCCTCTTCTCCTCCAATTCCGAGCAGCGATCCCGGGCCGAGCTCGTCTATAACCTCTGCAAAAAGTACGATCCCGACTCGCTCTCCCTCACACTCGCTCACCTCCTCCAATTCTCGCCGGCGCCGGACTCCCGATTTATCTCCGCCGTCCTCCTCCGCAATCAAATCCGCCTCTGGCCAAGCCTCTCGGAGAACACACAATCCACGATCAAATCCGCTCTATTGTTCTCCATTCAACGACAGGACAACACGAAATCGATTTCAGGCAGCCTCTGCGACGCCGTTTCTGCACTCGCCGCCGGGATCTTGCCGGAAAACGGCTGGCCGGAGCTCTTGCCCTTTCTGTTCGCATCCGTTTCCGCGGATTCGCTGCCGCCGATACTTCAGGAATCGGCGGCGTTCTTGATATTCGCGCAGCTCTCGCCGCACTTATTGCCGTATATAAACGAATTGCGCACAGTCTTCTTGTCCTGCTTGAGCTCCTCGTCCAGGTCAAACGACGTTAAAGTAGCTGCGTTTAATGCGGTGGTCAATTTCATTCGGTGTTTGCCCAGCTCTGAAGATCCGGATATGTTTCACGACGTCTTGATAGAAATGATGAGAACCGTGATGGGGGAATTGAACAAAAAGAACGAGGCCACGGCGCAGGAGGCGATCAAGTTGTTCATTGAATTGGCCGGGACCCATCCGACGTTGCTCAGCCGCAGGGGGCAGATCGTCCGGGCTATGCTGCAGATTGCTGAAGCCATACAAGTGTTGGAACAAGGCACGAGGCACTTGGCCATAGAGTTTGTGATCACTTTGGCCGAGGCAGGTGAGCAGGCGCCATGGATAATGAGGAATTCAGTGCAGTTTGTTGGTCTGTTGTTTTCCATTTTGATGAGGATGGTTTCGGATGTTGAGGATGATCCCTTGTGGCATAAGGCTGAGACTGATGATGAGTATGCGGGTGTCAGTGCTGATTACCGTGTTGGGAATGAGTGTTTGCATAGGCTTGCCATTGCGTTGGGCGGCAATGACATTGTTCCTGTTGCGTTGAAGGAGTTACCTGCCTGTTTTGCTTCCAATGAGTGGAAGAAGCACAATGCTGCACTGGTTGCCCTTGCTCAAATTTCTGAGGGTTGTTCAAAGGTAATGATAAAAGATTTGGAGCAAGTGGTGGCAATGGTTTTGAACTCCTTTGAACATTCACATATTCGGGTAAGGTGGTCAGCGATTAATGCAGTTGGCCAGTTGTCTACTTATTTGGCCCCAGACCTGCAAGTTCAATATCATCAACAGGTGTTTTCTGCACTGAATGCCACTATATATGGTTTGCAGAATCGTCGTCTGCTGGCACATGCTGCTTCAGCACTGCAAAAATTCATTGAGAAATGTGCTAGGGATATCTTGACACCTTATTTGGATGAAGTGTTTTCCCAATTGGTTGTACTTTTGCAGAGTGAGATACAAATGGTGCAAGTAGAAGCATTGACAGCTTTGGCATCGGTTGTTGTTTCATTTCCGGAGCAATTCCAAAAATACTATGAAGTTGTCATGATTCCCCTGAGAGCTATGTTGTTGGATGCCGCTAATAACCCAGTTTTGTCCAAGTCCGTGGCGTGTATTAGCTTGGTTGGTATGGCTGTTGGAAAGGAGAAATTCAGGGATGATGCTAAGAAAGTCATGGAAGTGCTGCTGTCTTTTCAAGGATCACAAATGGAGCCAGATGATCCAACTGCGAGTTGCATGCTACAGATATGCGCTGACCTGTGCAAGTGTCTAGGACAAGATTTCCTTCCTTACATGGGTGCTGTTATGCCTCCTTTGCTTCAGTTTGTTCAAATTAAGCCTGTTGAAACCGTTACCTCCGCAAATGACAATAGTGATATGGTTGAATCTGATGATGAAAGTATGGAAACAATTACTCTTGGAGATAAAAGGATTAGAATCAAGACTAGTGCATTGGAGGAAAAAGTTACATCTTGCAATTTGCTGTGTTGCTATGCTGATGAACTGAAGGAAGGATTCTTTCCATGGATCGATCAGGTTGCCCCAACGTTAGTTCCACTTATATCTTATATCCATGAAAAAGTGAGGAAGTCTGCTGTTTCAGCAATGCTGAAGCTACTGCTTACTGCAAAGTTAGCTGTAGACAGAGGGCAAGCTCAAGGTTGTAATGAAACGTATTTAAAGCAATTGTCTGACTACATCGTTCGGGCTCTGGTGGAAGCATTACCTAAGGAAGATGATTCTGAAATCTGCACAAAGATATTGGATGCTATAAATGAATGCATACAGCTCTTTGGATCCACTCTAGATGAAAGCCTTGTCAAATCCATCAAGGAGATAAAGCAGGTGATTACTCCTCGTTCTAGTAAGAAAAGAGAGAGCAGAAAGGACCTAAGCATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000060 GO:0001101 GO:0003674 GO:0005048 GO:0005215 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008139 GO:0008150 GO:0008565 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0017038 GO:0031090 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032386 GO:0032388 GO:0032879 GO:0032880 GO:0033036 GO:0033157 GO:0033218 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0042221 GO:0042277 GO:0042306 GO:0042307 GO:0042886 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046822 GO:0046824 GO:0046907 GO:0048518 GO:0050789 GO:0050896 GO:0051049 GO:0051050 GO:0051169 GO:0051170 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0060341 GO:0065007 GO:0070013 GO:0070201 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071702 GO:0071705 GO:0072594 GO:0090087 GO:0090316 GO:1900180 GO:1900182 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1903827 GO:1903829 GO:1904589 GO:1904591 GO:1904951

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HEAT HEAT_2 HEAT_EZ

Protein Analysis

842

Amino Acids

93.29

Weight (kDa)

5.14

Isoelectric Point (pI)

45.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_IPO5	PF25780	132 - 280	2.4e-16	IPO5-like, TPR repeats
Importin_rep_4	PF18808	298 - 388	3.6e-14	Importin repeat
TPR_TNPO3_IPO13_3rd	PF24140	429 - 508	1e-05	Transportin-3/Importin-13 third TPR domain
TPR_IMB1	PF25574	491 - 657	2.6e-08	Importin subunit beta-1-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004551)

Species	Orthologous Gene IDs
malus_domestica	MD11G1005500.v1.1
rosa_chinensis	RchiOBHm_Chr5g0054081 RchiOBHm_Chr5g0054171 RchiOBHm_Chr5g0054201 RchiOBHm_Chr5g0054251
rosa_laevigata	RLG00000034915 RLG00000034916 RLG00000034918 RLG00000034920 RLG00000034921
rosa_multiflora	Rmu_sc0000094.1_g000006 Rmu_sc0000509.1_g000025 Rmu_sc0006889.1_g000007 Rmu_sc0010082.1_g000001 Rmu_sc0023670.1_g000001
rosa_roxburghii	Rroxscaffold_1G00026650 Rroxscaffold_1G00026670
rosa_rugosa	Rorug05G0285300
rosa_samantha	Rh5AG354200 Rh5AG354300 Rh5AG354700 Rh5AG355200 Rh5BG366000 Rh5BG366100 Rh5BG367300 Rh5CG390800 Rh5DG055000 Rh5DG380900 Rh5DG381500
rosa_wichuraiana	Rw5G033390 Rw5G033410 Rw5G033590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	938, 2461
AatII	GACGTC	1 cut(s)	720
Acc16I	TGCGCA	1 cut(s)	590
Acc36I	ACCTGC	4 cut(s)	938, 1192, 1401, 2461
AccB1I	GGYRCC	2 cut(s)	256, 958
AccB7I	CCANNNNNTGG	1 cut(s)	1206
AccBSI	CCGCTC	2 cut(s)	67, 353
AccI	GTMKAC	3 cut(s)	11, 1373, 2268
AccII	CGCG	3 cut(s)	44, 497, 548
AccIII	TCCGGA	2 cut(s)	700, 1639
AclI	AACGTT	1 cut(s)	2174
AcoI	YGGCCR	7 cut(s)	3, 311, 458, 810, 915, 939, 1363
AcsI	RAATTY	4 cut(s)	976, 1245, 1496, 1775
AcuI	CTGAAG	7 cut(s)	503, 714, 897, 1467, 1931, 2153, 2258
AcyI	GRCGYC	4 cut(s)	257, 414, 717, 959
AfaI	GTAC	2 cut(s)	203, 1566
AfiI	CCNNNNNNNGG	5 cut(s)	452, 624, 747, 1206, 1922
AhlI	ACTAGT	1 cut(s)	2072
AjnI	CCWGG	1 cut(s)	623
AloI	GAACNNNNNNTCC	2 cut(s)	320, 352
Alw21I	GWGCWC	3 cut(s)	180, 468, 617
Alw26I	GTCTC	1 cut(s)	1058
AlwNI	CAGNNNCTG	1 cut(s)	408
Ama87I	CYCGRG	1 cut(s)	168
Aor13HI	TCCGGA	2 cut(s)	700, 1639
ApeKI	GCWGC	9 cut(s)	160, 402, 505, 550, 647, 865, 1223, 1478, 1810
ApoI	RAATTY	4 cut(s)	976, 1245, 1496, 1775
ArsI	GACNNNNNNTTYG	2 cut(s)	1519, 1551
AseI	ATTAAT	1 cut(s)	1353
AspLEI	GCGC	6 cut(s)	259, 550, 591, 781, 961, 1880
AspS9I	GGNCC	5 cut(s)	56, 171, 816, 1384, 2515
AsuC2I	CCSGG	5 cut(s)	169, 170, 436, 814, 857
AsuHPI	GGTGA	3 cut(s)	227, 962, 2485
AvaI	CYCGRG	1 cut(s)	168
AvaII	GGWCC	2 cut(s)	816, 2515
AxyI	CCTNAGG	1 cut(s)	2355
BalI	TGGCCA	3 cut(s)	313, 917, 1365
BamHI	GGATCC	1 cut(s)	2423
BanI	GGYRCC	2 cut(s)	256, 958
BanII	GRGCYC	4 cut(s)	180, 468, 617, 2338
BauI	CACGAG	1 cut(s)	904
BbsI	GAAGAC	1 cut(s)	589
Bbv12I	GWGCWC	3 cut(s)	180, 468, 617
BbvI	GCAGC	9 cut(s)	172, 414, 492, 562, 634, 852, 1210, 1465, 1797
BccI	CCATC	4 cut(s)	739, 828, 1015, 2462
BceAI	ACGGC	5 cut(s)	266, 401, 469, 556, 792
BcgI	CGANNNNNNTGC	6 cut(s)	1442, 1476, 1846, 1880, 2147, 2181
BciT130I	CCWGG	1 cut(s)	625
BclI	TGATCA	1 cut(s)	930
BcnI	CCSGG	5 cut(s)	169, 170, 436, 814, 857
BcoDI	GTCTC	1 cut(s)	1058
BcuI	ACTAGT	1 cut(s)	2072
BfaI	CTAG	5 cut(s)	1515, 1898, 2073, 2432, 2490
BfmI	CTRYAG	3 cut(s)	866, 1868, 2265
BfoI	RGCGCY	2 cut(s)	260, 962
BfuAI	ACCTGC	4 cut(s)	938, 1192, 1401, 2461
BlpI	GCTNAGC	1 cut(s)	833
Bme1390I	CCNGG	6 cut(s)	169, 170, 436, 625, 814, 857
Bme18I	GGWCC	2 cut(s)	816, 2515
BmeT110I	CYCGRG	1 cut(s)	168
BmgT120I	GGNCC	5 cut(s)	56, 171, 816, 1384, 2515
BmiI	GGNNCC	8 cut(s)	58, 258, 817, 818, 960, 1386, 1839, 2425
BmrFI	CCNGG	6 cut(s)	169, 170, 436, 625, 814, 857
BmrI	ACTGGG	1 cut(s)	1709
BmsI	GCATC	5 cut(s)	494, 1628, 1690, 1777, 2383
BmuI	ACTGGG	1 cut(s)	1709
BpiI	GAAGAC	1 cut(s)	589
BpmI	CTGGAG	1 cut(s)	5
Bpu10I	CCTNAGC	1 cut(s)	2519
Bpu1102I	GCTNAGC	1 cut(s)	833
BpuEI	CTTGAG	3 cut(s)	53, 631, 2268
BpuMI	CCSGG	5 cut(s)	169, 170, 436, 814, 857
Bsa29I	ATCGAT	2 cut(s)	392, 2157
BsaBI	GATNNNNATC	1 cut(s)	2063
BsaHI	GRCGYC	4 cut(s)	257, 414, 717, 959
BsaJI	CCNNGG	7 cut(s)	168, 495, 774, 942, 962, 1731, 2150
BsaWI	WCCGGW	2 cut(s)	700, 1639
BsaXI	ACNNNNNCTCC	2 cut(s)	203, 233
Bsc4I	CCNNNNNNNGG	5 cut(s)	452, 624, 747, 1206, 1922
Bse118I	RCCGGY	1 cut(s)	253
Bse1I	ACTGG	3 cut(s)	1233, 1366, 1715
Bse21I	CCTNAGG	1 cut(s)	2355
Bse3DI	GCAATG	4 cut(s)	1134, 1156, 1305, 2238
Bse8I	GATNNNNATC	1 cut(s)	2063
BseAI	TCCGGA	2 cut(s)	700, 1639
BseBI	CCWGG	1 cut(s)	625
BseCI	ATCGAT	2 cut(s)	392, 2157
BseDI	CCNNGG	7 cut(s)	168, 495, 774, 942, 962, 1731, 2150
BseGI	GGATG	9 cut(s)	485, 820, 1026, 1036, 1045, 1546, 1705, 1789, 2398
BseJI	GATNNNNATC	1 cut(s)	2063
BseLI	CCNNNNNNNGG	5 cut(s)	452, 624, 747, 1206, 1922
BseMI	GCAATG	4 cut(s)	1134, 1156, 1305, 2238
BseMII	CTCAG	5 cut(s)	29, 847, 1053, 1242, 1673
BseNI	ACTGG	3 cut(s)	1233, 1366, 1715
BseRI	GAGGAG	8 cut(s)	89, 121, 124, 136, 230, 278, 607, 2472
BseXI	GCAGC	9 cut(s)	172, 414, 492, 562, 634, 852, 1210, 1465, 1797
BseYI	CCCAGC	1 cut(s)	686
BsgI	GTGCAG	5 cut(s)	408, 1004, 1209, 1410, 2362
Bsh1236I	CGCG	3 cut(s)	44, 497, 548
BshNI	GGYRCC	2 cut(s)	256, 958
BshVI	ATCGAT	2 cut(s)	392, 2157
BsiHKAI	GWGCWC	3 cut(s)	180, 468, 617
BsiHKCI	CYCGRG	1 cut(s)	168
BslFI	GGGAC	1 cut(s)	829
BslI	CCNNNNNNNGG	5 cut(s)	452, 624, 747, 1206, 1922
BsmAI	GTCTC	1 cut(s)	1058
BsmFI	GGGAC	1 cut(s)	829
BsmI	GAATGC	2 cut(s)	1438, 2411
BsoBI	CYCGRG	1 cut(s)	168
Bsp1286I	GDGCHC	4 cut(s)	180, 468, 617, 2338
Bsp13I	TCCGGA	2 cut(s)	700, 1639
Bsp1720I	GCTNAGC	1 cut(s)	833
Bsp19I	CCATGG	2 cut(s)	962, 2150
Bsp68I	TCGCGA	1 cut(s)	44
BspCNI	CTCAG	5 cut(s)	28, 846, 1054, 1243, 1674
BspDI	ATCGAT	2 cut(s)	392, 2157
BspEI	TCCGGA	2 cut(s)	700, 1639
BspFNI	CGCG	3 cut(s)	44, 497, 548
BspHI	TCATGA	1 cut(s)	1671
BspLI	GGNNCC	8 cut(s)	58, 258, 817, 818, 960, 1386, 1839, 2425
BspMAI	CTGCAG	1 cut(s)	870
BspMI	ACCTGC	4 cut(s)	938, 1192, 1401, 2461
BspT107I	GGYRCC	2 cut(s)	256, 958
BsrBI	CCGCTC	2 cut(s)	67, 353
BsrDI	GCAATG	4 cut(s)	1134, 1156, 1305, 2238
BsrFI	RCCGGY	1 cut(s)	253
BsrI	ACTGG	3 cut(s)	1233, 1366, 1715
BssAI	RCCGGY	1 cut(s)	253
BssECI	CCNNGG	7 cut(s)	168, 495, 774, 942, 962, 1731, 2150
BssNI	GRCGYC	4 cut(s)	257, 414, 717, 959
BssSI	CACGAG	1 cut(s)	904
BssT1I	CCWWGG	2 cut(s)	962, 2150
Bst2BI	CACGAG	1 cut(s)	904
Bst2UI	CCWGG	1 cut(s)	625
Bst4CI	ACNGT	4 cut(s)	595, 742, 1103, 1978
Bst6I	CTCTTC	1 cut(s)	146
BstACI	GRCGYC	4 cut(s)	257, 414, 717, 959
BstAPI	GCANNNNNTGC	5 cut(s)	408, 568, 1232, 1433, 1478
BstC8I	GCNNGC	8 cut(s)	69, 255, 458, 957, 1131, 1470, 1865, 2280
BstDEI	CTNAG	8 cut(s)	15, 833, 1062, 1251, 1682, 1791, 2355, 2519
BstDSI	CCRYGG	5 cut(s)	495, 774, 962, 1731, 2150
BstENI	CCTNNNNNAGG	1 cut(s)	622
BstF5I	GGATG	9 cut(s)	485, 820, 1026, 1036, 1045, 1546, 1705, 1789, 2398
BstFNI	CGCG	3 cut(s)	44, 497, 548
BstH2I	RGCGCY	2 cut(s)	260, 962
BstHHI	GCGC	6 cut(s)	259, 550, 591, 781, 961, 1880
BstMAI	GTCTC	1 cut(s)	1058
BstNI	CCWGG	1 cut(s)	625
BstNSI	RCATGY	2 cut(s)	1478, 1867
BstSCI	CCNGG	6 cut(s)	167, 168, 434, 623, 812, 855
BstSFI	CTRYAG	3 cut(s)	866, 1868, 2265
BstUI	CGCG	3 cut(s)	44, 497, 548
BstV1I	GCAGC	9 cut(s)	172, 414, 492, 562, 634, 852, 1210, 1465, 1797
BstV2I	GAAGAC	1 cut(s)	589
BstX2I	RGATCY	3 cut(s)	438, 697, 2423
BstXI	CCANNNNNNTGG	2 cut(s)	1141, 1732
BstYI	RGATCY	3 cut(s)	438, 697, 2423
Bsu15I	ATCGAT	2 cut(s)	392, 2157
Bsu36I	CCTNAGG	1 cut(s)	2355
BsuTUI	ATCGAT	2 cut(s)	392, 2157
BtgI	CCRYGG	5 cut(s)	495, 774, 962, 1731, 2150
BtsCI	GGATG	9 cut(s)	485, 820, 1026, 1036, 1045, 1546, 1705, 1789, 2398
BtsI	GCAGTG	1 cut(s)	1487
BtsIMutI	CAGTG	5 cut(s)	987, 1096, 1226, 1427, 1487
BtuMI	TCGCGA	1 cut(s)	44
BveI	ACCTGC	4 cut(s)	938, 1192, 1401, 2461
Cac8I	GCNNGC	8 cut(s)	69, 255, 458, 957, 1131, 1470, 1865, 2280
CaiI	CAGNNNCTG	1 cut(s)	408
CciI	TCATGA	1 cut(s)	1671
CfoI	GCGC	6 cut(s)	259, 550, 591, 781, 961, 1880
Cfr10I	RCCGGY	1 cut(s)	253
Cfr13I	GGNCC	5 cut(s)	56, 171, 816, 1384, 2515
Cfr42I	CCGCGG	1 cut(s)	498
Cfr9I	CCCGGG	1 cut(s)	168
ClaI	ATCGAT	2 cut(s)	392, 2157
CseI	GACGC	1 cut(s)	422
Csp6I	GTAC	2 cut(s)	202, 1565
CviAII	CATG	8 cut(s)	963, 1475, 1672, 1801, 1864, 1921, 2151, 2201
CviQI	GTAC	2 cut(s)	202, 1565
DdeI	CTNAG	8 cut(s)	15, 833, 1062, 1251, 1682, 1791, 2355, 2519
DinI	GGCGCC	2 cut(s)	258, 960
DraI	TTTAAA	1 cut(s)	2307
EaeI	YGGCCR	7 cut(s)	3, 311, 458, 810, 915, 939, 1363
Eam1104I	CTCTTC	1 cut(s)	146
EarI	CTCTTC	1 cut(s)	146
EciI	GGCGGA	2 cut(s)	268, 293
Ecl136II	GAGCTC	3 cut(s)	178, 466, 615
Eco130I	CCWWGG	2 cut(s)	962, 2150
Eco24I	GRGCYC	4 cut(s)	180, 468, 617, 2338
Eco32I	GATATC	1 cut(s)	1522
Eco47I	GGWCC	2 cut(s)	816, 2515
Eco53kI	GAGCTC	3 cut(s)	178, 466, 615
Eco57I	CTGAAG	7 cut(s)	503, 714, 897, 1467, 1931, 2153, 2258
Eco81I	CCTNAGG	1 cut(s)	2355
Eco88I	CYCGRG	1 cut(s)	168
EcoICRI	GAGCTC	3 cut(s)	178, 466, 615
EcoNI	CCTNNNNNAGG	1 cut(s)	622
EcoO109I	RGGNCCY	2 cut(s)	816, 2515
EcoRI	GAATTC	1 cut(s)	976
EcoRII	CCWGG	1 cut(s)	623
EcoRV	GATATC	1 cut(s)	1522
EcoT14I	CCWWGG	2 cut(s)	962, 2150
EcoT22I	ATGCAT	1 cut(s)	2411
EcoT38I	GRGCYC	4 cut(s)	180, 468, 617, 2338
EgeI	GGCGCC	2 cut(s)	258, 960
EheI	GGCGCC	2 cut(s)	258, 960
ErhI	CCWWGG	2 cut(s)	962, 2150
FaeI	CATG	8 cut(s)	966, 1478, 1675, 1804, 1867, 1924, 2154, 2204
FalI	AAGNNNNNCTT	2 cut(s)	1896, 1928
FaqI	GGGAC	1 cut(s)	829
FatI	CATG	8 cut(s)	962, 1474, 1671, 1800, 1863, 1920, 2150, 2200
FauI	CCCGC	2 cut(s)	72, 1075
FauNDI	CATATG	1 cut(s)	2525
FbaI	TGATCA	1 cut(s)	930
FblI	GTMKAC	3 cut(s)	11, 1373, 2268
FokI	GGATG	9 cut(s)	472, 807, 1033, 1043, 1052, 1553, 1712, 1796, 2405
FriOI	GRGCYC	4 cut(s)	180, 468, 617, 2338
FspBI	CTAG	5 cut(s)	1515, 1898, 2073, 2432, 2490
FspI	TGCGCA	1 cut(s)	590
GlaI	GCGC	6 cut(s)	258, 549, 590, 780, 960, 1879
GsaI	CCCAGC	1 cut(s)	690
GsuI	CTGGAG	1 cut(s)	5
HaeII	RGCGCY	2 cut(s)	260, 962
HgaI	GACGC	1 cut(s)	422
HhaI	GCGC	6 cut(s)	259, 550, 591, 781, 961, 1880
Hin1I	GRCGYC	4 cut(s)	257, 414, 717, 959
Hin1II	CATG	8 cut(s)	966, 1478, 1675, 1804, 1867, 1924, 2154, 2204
Hin6I	GCGC	6 cut(s)	257, 548, 589, 779, 959, 1878
HinP1I	GCGC	6 cut(s)	257, 548, 589, 779, 959, 1878
HincII	GTYRAC	1 cut(s)	12
HindII	GTYRAC	1 cut(s)	12
HphI	GGTGA	3 cut(s)	227, 962, 2485
Hpy166II	GTNNAC	3 cut(s)	12, 1374, 2269
Hpy188I	TCNGA	9 cut(s)	157, 325, 694, 825, 1030, 1252, 2017, 2320, 2371
Hpy8I	GTNNAC	3 cut(s)	12, 1374, 2269
Hpy99I	CGWCG	6 cut(s)	49, 416, 638, 719, 829, 1464
HpyAV	CCTTC	4 cut(s)	1168, 1922, 2128, 2132
HpyCH4III	ACNGT	4 cut(s)	595, 742, 1103, 1978
HpyCH4IV	ACGT	5 cut(s)	636, 717, 827, 2174, 2300
HpyF3I	CTNAG	8 cut(s)	15, 833, 1062, 1251, 1682, 1791, 2355, 2519
HpySE526I	ACGT	5 cut(s)	636, 717, 827, 2174, 2300
Hsp92I	GRCGYC	4 cut(s)	257, 414, 717, 959
Hsp92II	CATG	8 cut(s)	966, 1478, 1675, 1804, 1867, 1924, 2154, 2204
HspAI	GCGC	6 cut(s)	257, 548, 589, 779, 959, 1878
KasI	GGCGCC	2 cut(s)	256, 958
KflI	GGGWCCC	1 cut(s)	816
Kpn2I	TCCGGA	2 cut(s)	700, 1639
KroI	GCCGGC	1 cut(s)	253
KroNI	GCCGGC	1 cut(s)	255
Ksp22I	TGATCA	1 cut(s)	930
KspI	CCGCGG	1 cut(s)	498
LmnI	GCTCC	6 cut(s)	24, 463, 620, 1285, 1642, 1837
Lsp1109I	GCAGC	9 cut(s)	172, 414, 492, 562, 634, 852, 1210, 1465, 1797
LweI	GCATC	5 cut(s)	494, 1628, 1690, 1777, 2383
MaeI	CTAG	5 cut(s)	1515, 1898, 2073, 2432, 2490
MaeII	ACGT	5 cut(s)	636, 717, 827, 2174, 2300
MaeIII	GTNAC	3 cut(s)	1179, 1978, 2092
MbiI	CCGCTC	2 cut(s)	67, 353
MboII	GAAGA	5 cut(s)	133, 589, 707, 1222, 2372
MfeI	CAATTG	2 cut(s)	1556, 2312
MflI	RGATCY	3 cut(s)	438, 697, 2423
MhlI	GDGCHC	4 cut(s)	180, 468, 617, 2338
MlsI	TGGCCA	3 cut(s)	313, 917, 1365
MluNI	TGGCCA	3 cut(s)	313, 917, 1365
Mly113I	GGCGCC	2 cut(s)	257, 959
MlyI	GAGTC	4 cut(s)	7, 17, 206, 257
MmeI	TCCRAC	5 cut(s)	848, 873, 1677, 1744, 1874
Mox20I	TGGCCA	3 cut(s)	313, 917, 1365
Mph1103I	ATGCAT	1 cut(s)	2411
MreI	CGCCGGCG	1 cut(s)	253
MroI	TCCGGA	2 cut(s)	700, 1639
MroNI	GCCGGC	1 cut(s)	253
MscI	TGGCCA	3 cut(s)	313, 917, 1365
MseI	TTAA	5 cut(s)	639, 654, 1353, 1962, 2306
MslI	CAYNNNNRTG	3 cut(s)	887, 1013, 1805
Msp20I	TGGCCA	3 cut(s)	313, 917, 1365
MspA1I	CMGCKG	1 cut(s)	497
MspR9I	CCNGG	6 cut(s)	169, 170, 436, 625, 814, 857
MunI	CAATTG	2 cut(s)	1556, 2312
Mva1269I	GAATGC	2 cut(s)	1438, 2411
MvaI	CCWGG	1 cut(s)	625
MvnI	CGCG	3 cut(s)	44, 497, 548
NaeI	GCCGGC	1 cut(s)	255
NarI	GGCGCC	2 cut(s)	257, 959
NciI	CCSGG	5 cut(s)	169, 170, 436, 814, 857
NcoI	CCATGG	2 cut(s)	962, 2150
NdeI	CATATG	1 cut(s)	2525
NgoMIV	GCCGGC	1 cut(s)	253
NlaIII	CATG	8 cut(s)	966, 1478, 1675, 1804, 1867, 1924, 2154, 2204
NlaIV	GGNNCC	8 cut(s)	58, 258, 817, 818, 960, 1386, 1839, 2425
NmeAIII	GCCGAG	3 cut(s)	31, 199, 967
NruI	TCGCGA	1 cut(s)	44
NsbI	TGCGCA	1 cut(s)	590
NsiI	ATGCAT	1 cut(s)	2411
NspI	RCATGY	2 cut(s)	1478, 1867
PaeI	GCATGC	1 cut(s)	1867
PagI	TCATGA	1 cut(s)	1671
PaqCI	CACCTGC	2 cut(s)	938, 2461
PcsI	WCGNNNNNNNCGW	1 cut(s)	509
PctI	GAATGC	2 cut(s)	1438, 2411
PdiI	GCCGGC	1 cut(s)	255
PfeI	GAWTC	9 cut(s)	49, 500, 524, 1456, 1675, 2012, 2064, 2142, 2366
PflMI	CCANNNNNTGG	1 cut(s)	1206
PleI	GAGTC	4 cut(s)	7, 16, 206, 257
PluTI	GGCGCC	2 cut(s)	260, 962
PpsI	GAGTC	4 cut(s)	7, 16, 206, 257
PpuMI	RGGWCCY	2 cut(s)	816, 2515
PshBI	ATTAAT	1 cut(s)	1353
Psp124BI	GAGCTC	3 cut(s)	180, 468, 617
Psp1406I	AACGTT	1 cut(s)	2174
Psp5II	RGGWCCY	2 cut(s)	816, 2515
Psp6I	CCWGG	1 cut(s)	623
PspFI	CCCAGC	1 cut(s)	686
PspGI	CCWGG	1 cut(s)	623
PspN4I	GGNNCC	8 cut(s)	58, 258, 817, 818, 960, 1386, 1839, 2425
PspPI	GGNCC	5 cut(s)	56, 171, 816, 1384, 2515
PspPPI	RGGWCCY	2 cut(s)	816, 2515
PstI	CTGCAG	1 cut(s)	870
PstNI	CAGNNNCTG	1 cut(s)	408
PsuI	RGATCY	3 cut(s)	438, 697, 2423
RruI	TCGCGA	1 cut(s)	44
RsaI	GTAC	2 cut(s)	203, 1566
RsaNI	GTAC	2 cut(s)	202, 1565
RseI	CAYNNNNRTG	3 cut(s)	887, 1013, 1805
SacI	GAGCTC	3 cut(s)	180, 468, 617
SacII	CCGCGG	1 cut(s)	498
SalI	GTCGAC	1 cut(s)	10
SaqAI	TTAA	5 cut(s)	639, 654, 1353, 1962, 2306
Sau96I	GGNCC	5 cut(s)	56, 171, 816, 1384, 2515
SchI	GAGTC	4 cut(s)	7, 17, 206, 257
ScrFI	CCNGG	6 cut(s)	169, 170, 436, 625, 814, 857
SduI	GDGCHC	4 cut(s)	180, 468, 617, 2338
SfaNI	GCATC	5 cut(s)	494, 1628, 1690, 1777, 2383
SfcI	CTRYAG	3 cut(s)	866, 1868, 2265
SfoI	GGCGCC	2 cut(s)	258, 960
Sfr303I	CCGCGG	1 cut(s)	498
SgrAI	CRCCGGYG	1 cut(s)	253
SgrBI	CCGCGG	1 cut(s)	498
SinI	GGWCC	2 cut(s)	816, 2515
SmaI	CCCGGG	1 cut(s)	170
SmiMI	CAYNNNNRTG	3 cut(s)	887, 1013, 1805
SmlI	CTYRAG	3 cut(s)	32, 610, 2283
SmoI	CTYRAG	3 cut(s)	32, 610, 2283
SpeI	ACTAGT	1 cut(s)	2072
SphI	GCATGC	1 cut(s)	1867
SspDI	GGCGCC	2 cut(s)	256, 958
SspMI	CTAG	5 cut(s)	1515, 1898, 2073, 2432, 2490
SstI	GAGCTC	3 cut(s)	180, 468, 617
StyD4I	CCNGG	6 cut(s)	167, 168, 434, 623, 812, 855
StyI	CCWWGG	2 cut(s)	962, 2150
TaaI	ACNGT	4 cut(s)	595, 742, 1103, 1978
TaiI	ACGT	5 cut(s)	639, 720, 830, 2177, 2303
TaqI	TCGA	3 cut(s)	11, 392, 2157
TatI	WGTACW	1 cut(s)	1564
TauI	GCSGC	7 cut(s)	434, 511, 533, 562, 840, 1149, 1706
TfiI	GAWTC	9 cut(s)	49, 500, 524, 1456, 1675, 2012, 2064, 2142, 2366
Tru1I	TTAA	5 cut(s)	639, 654, 1353, 1962, 2306
Tru9I	TTAA	5 cut(s)	639, 654, 1353, 1962, 2306
TscAI	CASTG	5 cut(s)	987, 1096, 1233, 1434, 1494
TseI	GCWGC	9 cut(s)	160, 402, 505, 550, 647, 865, 1223, 1478, 1810
TspGWI	ACGGA	2 cut(s)	478, 1720
TspMI	CCCGGG	1 cut(s)	168
TspRI	CASTG	5 cut(s)	987, 1096, 1233, 1434, 1494
Van91I	CCANNNNNTGG	1 cut(s)	1206
VpaK11BI	GGWCC	2 cut(s)	816, 2515
VspI	ATTAAT	1 cut(s)	1353
XagI	CCTNNNNNAGG	1 cut(s)	622
XapI	RAATTY	4 cut(s)	976, 1245, 1496, 1775
XbaI	TCTAGA	1 cut(s)	2431
XceI	RCATGY	2 cut(s)	1478, 1867
XcmI	CCANNNNNNNNNTGG	1 cut(s)	889
XmaI	CCCGGG	1 cut(s)	168
XmiI	GTMKAC	3 cut(s)	11, 1373, 2268
XspI	CTAG	5 cut(s)	1515, 1898, 2073, 2432, 2490
ZraI	GACGTC	1 cut(s)	718
Zsp2I	ATGCAT	1 cut(s)	2411

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.