Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0004551

Physical Location & Seq

Genomic Coordinates Reverse (-)

33612302 .. 33618394

Gene Structure 6093 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00026670.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2490 bp

ATGGCCGAGTCGACTCAGCTCCAGCTTCAACTTGAGCAAGTCGCGACGATTCTTGGCCCCGACCCGCTCGCCTCTTTCAAAACCCTAATCTCCTGCCTCCTCTGCCCCTCCTATTTTGAAAGCCTAATCTCCTCCTCCCTCTTCTCCTCCAATTCCGAGCAGCGATGCCGGGCCGAGCTCGTCTATAACCTCTGCAAAAAGTACGATCCCGACTCGCTCTCCCTCACACTCGCTCACCTCCTCCAATTCTCGCCGGCGCACGACTCCCGATTTTTCTCCGCCGTCCTCCTCCGCAATCAAATCCGCCTCTGGCCAAGCCTCTCGGAGAACACACAATCCACGATCAAATCCGTTCTATTGTTCTCCATTCAACGACAGGACAACACGAAATTGATTTCAGGCAACCTCTGCGACGCCGTTTCGGCGCTCGCCGCCGGAATCTTGCCGGAAAACTGCTGGCCGGAGCTCTTGCCCTTTCTGTTCGCATCCGCTTCCGCGGATTCGTTGCCGCCGATACTTCAGGAATCGGCAGCGTTCTTGATATTCGCGCAGCTGTCGCCGCACTTATTGCAGTATATAAACGAATTGCGCACAGTCTTCTTGTACTGCTTGAGCTCCTCCACAAGGTCAGATGAAGTTAAGATAGCTGCGTTTAGTGCTGTGGTCAATTTCATTCGGTGTTTGCCCTACTCTGAAGATGAGGATAGGTTTCACGACGTCTTGATAGAAATGATGAGAACAGTGATGGGGGAATTGAACAAAGGGAAGGAGATCATGGCACAGGAGGCGATCAAGTTGTTCATTGAATTGGCCGGGACCCATCCGACGTTGCTCAGCCGCAACGGGCAGATCATCCGGGCTATGCTGCAGATTGCTGAAGCCATAGATGTCTTGGAACAAGGGACGAGGCACTTGGCCATTGAGTTTCTGATTACTCTGGCCGAGGCCGGAGAGCGTGCACCATGGATAATGAGGAATTCAGTGCAGTTTGTTGGTCTGTTGTTTTCCATTTTGATGAGGATGGTTTCGGATGTTGAGGATGATCCCTTGTGGCATAACGCTGAGACTGATGATGAGTATGCGGGTGTAAGTGCTGATTACCGTGTTGGGAATGAGTGTTTGCATAGGCTTGCCATTGCGTTGGGTGGCAATGACATTGTTCCTGTTGCGTTGAAGGAGTTACCTGCCTGTTTTGCTTCCAATGAGTGGAAGAAGCACAATGCTGCACTGGTTGCCCTTGCTCAAATTTCTGAGGGTTGTTCAAAGGTAATGATAAAAGATTTGGAGCAAGTGGTGGCAATGGTTTTGAACTCCTTTGAACATCCACATATTCGGGTAAGGTGGTCAGCGATTAATGCAGTTGGCCAGTTGTCTACTTATTTGGCCCCAGACCTGCAAGTTCAATATCATCAACAGGTGTTTTCTGCACTGAATGCCACCATATATGGTTTGCAGAACCGTCGTCTGCTGGCAAATGCTGCTTCAGCACTGCAAAAATTCATTGAGAAATGTGCTAGGGATATCTTGACACCTTATTTGGATGAAGTGGTTTCCCAATTGGTTGTACTTTTGCAGAGTGAGATACAAATGGTGAAAGTAGAAGCATTGACTGCTTTGGCATCAGTTGTTGTTTCATTTCCGGAGCAATTCCAAAAATACTATGAAGTTGTCATGATTCCCCTGAGAGCTATGTTGTTGGATGCCACTAATAACCTGGTTTGGTCCAAGTCTGTGGAATGTATTAGCTTGGTTGGTATGGCTGTTGGAAAGGAGAAATTCAGGGATGATGCTAAGAAAGTCATGGAAGTGCTGATCTCTTCGCAAGGATTTCAAATGGAGGCAGATGACCCAACTACAAGTTGCATGCTACAGACATGTGTTGACCTCTGCAAGTGTTTAGGACAAGATTTTCTCCCTTACGTGAGTATTGTAATGCCTCCTTTGCTTCAGTTTGCTCAACTTAAGCCAGATGAAACTATTACATTCACAAATTACAGTAGTGATATTGATGAATCTGAAGATGAAAGTATGGAAGCAATTACTCTTGGAGATAATAGAATTAGAATCAAGACTAGTCTAGTGGAGGAAAAAGTAGCAGCTTGTAACTTGCTGTGGTACTGTGCTGATGAATTGAAAGAAGAACTCTTTCCATGGATTGATCAGGTTGCTACAACTTTAGTTCCACTTATATCTTATATCCATGAAAAAGTGAGGAAGTCAGCTGTTTCAGCAATGCTGAAGCTACTGCTTTCTGCAAAGTTAGCTATAGAGAAAAGACAAGCTCAAGGATGTAATGAAACCTATATGAAGAAATTGTCTGACCACATCCTCCCAGCTCTGGCGGAAGCATCACATCAGGAACCTGATACAGAAATCACTGGAATAATATTGGATGCTATAAATGAATGCAGACAGATATCTGGACCACTTCGAGATGAAGCCATGTCACATGCACTTAGTCGAGGAGATAAAGCAGGGATCACTCCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000060 GO:0001101 GO:0003674 GO:0005048 GO:0005215 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008139 GO:0008150 GO:0008565 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0017038 GO:0031090 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032386 GO:0032388 GO:0032879 GO:0032880 GO:0033036 GO:0033157 GO:0033218 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0042221 GO:0042277 GO:0042306 GO:0042307 GO:0042886 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046822 GO:0046824 GO:0046907 GO:0048518 GO:0050789 GO:0050896 GO:0051049 GO:0051050 GO:0051169 GO:0051170 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0060341 GO:0065007 GO:0070013 GO:0070201 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071702 GO:0071705 GO:0072594 GO:0090087 GO:0090316 GO:1900180 GO:1900182 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1903827 GO:1903829 GO:1904589 GO:1904591 GO:1904951

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HEAT HEAT_2 HEAT_EZ

Protein Analysis

829

Amino Acids

92.3

Weight (kDa)

4.97

Isoelectric Point (pI)

45.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_IPO5	PF25780	135 - 280	8.7e-16	IPO5-like, TPR repeats
Importin_rep_4	PF18808	298 - 388	1.5e-14	Importin repeat
TOR1L1_N	PF24714	374 - 546	4.9e-06	TORTIFOLIA1 N-terminal
TPR_IMB1	PF25574	489 - 672	7.1e-10	Importin subunit beta-1-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004551)

Species	Orthologous Gene IDs
malus_domestica	MD11G1005500.v1.1
rosa_chinensis	RchiOBHm_Chr5g0054081 RchiOBHm_Chr5g0054171 RchiOBHm_Chr5g0054201 RchiOBHm_Chr5g0054251
rosa_laevigata	RLG00000034915 RLG00000034916 RLG00000034918 RLG00000034920 RLG00000034921
rosa_multiflora	Rmu_sc0000094.1_g000006 Rmu_sc0000509.1_g000025 Rmu_sc0006889.1_g000007 Rmu_sc0010082.1_g000001 Rmu_sc0023670.1_g000001
rosa_roxburghii	Rroxscaffold_1G00026650 Rroxscaffold_1G00026670
rosa_rugosa	Rorug05G0285300
rosa_samantha	Rh5AG354200 Rh5AG354300 Rh5AG354700 Rh5AG355200 Rh5BG366000 Rh5BG366100 Rh5BG367300 Rh5CG390800 Rh5DG055000 Rh5DG380900 Rh5DG381500
rosa_wichuraiana	Rw5G033390 Rw5G033410 Rw5G033590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	720
Acc16I	TGCGCA	1 cut(s)	590
Acc36I	ACCTGC	2 cut(s)	1192, 1401
AccB7I	CCANNNNNTGG	1 cut(s)	1206
AccBSI	CCGCTC	1 cut(s)	67
AccI	GTMKAC	2 cut(s)	11, 1373
AccII	CGCG	3 cut(s)	44, 497, 548
AccIII	TCCGGA	1 cut(s)	1639
AclWI	GGATC	3 cut(s)	200, 1037, 2486
AcoI	YGGCCR	7 cut(s)	3, 311, 458, 810, 915, 939, 1363
AcsI	RAATTY	4 cut(s)	976, 1245, 1496, 1775
AcuI	CTGAAG	7 cut(s)	503, 714, 897, 1467, 1931, 2037, 2258
AcyI	GRCGYC	2 cut(s)	414, 717
AfaI	GTAC	4 cut(s)	203, 605, 1566, 2117
AfiI	CCNNNNNNNGG	3 cut(s)	624, 1206, 2338
AflII	CTTAAG	1 cut(s)	1961
AflIII	ACRYGT	1 cut(s)	1874
AhlI	ACTAGT	1 cut(s)	2072
AjnI	CCWGG	1 cut(s)	1713
AloI	GAACNNNNNNTCC	2 cut(s)	320, 352
Alw21I	GWGCWC	4 cut(s)	180, 468, 617, 961
Alw26I	GTCTC	1 cut(s)	1058
Alw44I	GTGCAC	1 cut(s)	957
AlwI	GGATC	3 cut(s)	200, 1037, 2486
Aor13HI	TCCGGA	1 cut(s)	1639
ApaLI	GTGCAC	1 cut(s)	957
ApeKI	GCWGC	8 cut(s)	160, 530, 550, 647, 865, 1223, 1478, 2096
ApoI	RAATTY	4 cut(s)	976, 1245, 1496, 1775
ArsI	GACNNNNNNTTYG	2 cut(s)	1519, 1551
AseI	ATTAAT	1 cut(s)	1353
Asp700I	GAANNNNTTC	1 cut(s)	2145
AspLEI	GCGC	4 cut(s)	259, 427, 550, 591
AspS9I	GGNCC	6 cut(s)	56, 171, 816, 1384, 1722, 2423
AsuC2I	CCSGG	3 cut(s)	170, 814, 857
AsuHPI	GGTGA	2 cut(s)	227, 1603
AvaII	GGWCC	3 cut(s)	816, 1722, 2423
BaeGI	GKGCMC	1 cut(s)	961
BalI	TGGCCA	3 cut(s)	313, 917, 1365
BanII	GRGCYC	3 cut(s)	180, 468, 617
BbsI	GAAGAC	1 cut(s)	589
Bbv12I	GWGCWC	4 cut(s)	180, 468, 617, 961
BbvI	GCAGC	8 cut(s)	172, 542, 562, 634, 852, 1210, 1465, 2108
BccI	CCATC	3 cut(s)	739, 828, 1015
BceAI	ACGGC	2 cut(s)	266, 401
BcgI	CGANNNNNNTGC	2 cut(s)	1442, 1476
BciT130I	CCWGG	1 cut(s)	1715
BclI	TGATCA	1 cut(s)	2158
BcnI	CCSGG	3 cut(s)	170, 814, 857
BcoDI	GTCTC	1 cut(s)	1058
BcuI	ACTAGT	1 cut(s)	2072
BfaI	CTAG	4 cut(s)	1515, 2073, 2078, 2488
BfmI	CTRYAG	3 cut(s)	866, 1868, 2265
BfoI	RGCGCY	1 cut(s)	428
BfrI	CTTAAG	1 cut(s)	1961
BfuAI	ACCTGC	2 cut(s)	1192, 1401
BglI	GCCNNNNNGGC	1 cut(s)	422
BlpI	GCTNAGC	1 cut(s)	833
Bme1390I	CCNGG	4 cut(s)	170, 814, 857, 1715
Bme18I	GGWCC	3 cut(s)	816, 1722, 2423
BmgT120I	GGNCC	6 cut(s)	56, 171, 816, 1384, 1722, 2423
BmiI	GGNNCC	5 cut(s)	58, 817, 818, 1386, 2361
BmrFI	CCNGG	4 cut(s)	170, 814, 857, 1715
BmsI	GCATC	7 cut(s)	155, 494, 1628, 1690, 1777, 2357, 2383
BpiI	GAAGAC	1 cut(s)	589
BpmI	CTGGAG	1 cut(s)	5
Bpu1102I	GCTNAGC	1 cut(s)	833
BpuEI	CTTGAG	3 cut(s)	53, 631, 2268
BpuMI	CCSGG	3 cut(s)	170, 814, 857
BsaAI	YACGTR	1 cut(s)	1921
BsaHI	GRCGYC	2 cut(s)	414, 717
BsaJI	CCNNGG	4 cut(s)	495, 942, 962, 2150
BsaWI	WCCGGW	1 cut(s)	1639
BsaXI	ACNNNNNCTCC	2 cut(s)	203, 233
Bsc4I	CCNNNNNNNGG	3 cut(s)	624, 1206, 2338
Bse118I	RCCGGY	1 cut(s)	253
Bse1I	ACTGG	3 cut(s)	1233, 1366, 2383
Bse3DI	GCAATG	4 cut(s)	1134, 1156, 1305, 2238
BseAI	TCCGGA	1 cut(s)	1639
BseBI	CCWGG	1 cut(s)	1715
BseDI	CCNNGG	4 cut(s)	495, 942, 962, 2150
BseLI	CCNNNNNNNGG	3 cut(s)	624, 1206, 2338
BseMI	GCAATG	4 cut(s)	1134, 1156, 1305, 2238
BseMII	CTCAG	5 cut(s)	29, 847, 1053, 1242, 1673
BseNI	ACTGG	3 cut(s)	1233, 1366, 2383
BseRI	GAGGAG	8 cut(s)	89, 121, 124, 136, 230, 278, 607, 2478
BseSI	GKGCMC	1 cut(s)	961
BseXI	GCAGC	8 cut(s)	172, 542, 562, 634, 852, 1210, 1465, 2108
BseYI	CCCAGC	1 cut(s)	2332
BsgI	GTGCAG	3 cut(s)	1004, 1209, 1410
Bsh1236I	CGCG	3 cut(s)	44, 497, 548
BsiHKAI	GWGCWC	4 cut(s)	180, 468, 617, 961
BsiSI	CCGG	9 cut(s)	169, 254, 435, 446, 461, 813, 856, 948, 1640
BslFI	GGGAC	2 cut(s)	829, 916
BslI	CCNNNNNNNGG	3 cut(s)	624, 1206, 2338
BsmAI	GTCTC	1 cut(s)	1058
BsmFI	GGGAC	2 cut(s)	829, 916
BsmI	GAATGC	2 cut(s)	1438, 2411
Bsp1286I	GDGCHC	4 cut(s)	180, 468, 617, 961
Bsp13I	TCCGGA	1 cut(s)	1639
Bsp143I	GATC	9 cut(s)	205, 342, 771, 789, 849, 1042, 1812, 2158, 2478
Bsp1720I	GCTNAGC	1 cut(s)	833
Bsp19I	CCATGG	2 cut(s)	962, 2150
Bsp68I	TCGCGA	1 cut(s)	44
BspCNI	CTCAG	5 cut(s)	28, 846, 1054, 1243, 1674
BspEI	TCCGGA	1 cut(s)	1639
BspFNI	CGCG	3 cut(s)	44, 497, 548
BspHI	TCATGA	1 cut(s)	1671
BspLI	GGNNCC	5 cut(s)	58, 817, 818, 1386, 2361
BspMAI	CTGCAG	1 cut(s)	870
BspMI	ACCTGC	2 cut(s)	1192, 1401
BspPI	GGATC	3 cut(s)	200, 1037, 2486
BspTI	CTTAAG	1 cut(s)	1961
BsrBI	CCGCTC	1 cut(s)	67
BsrDI	GCAATG	4 cut(s)	1134, 1156, 1305, 2238
BsrFI	RCCGGY	1 cut(s)	253
BsrI	ACTGG	3 cut(s)	1233, 1366, 2383
BssAI	RCCGGY	1 cut(s)	253
BssECI	CCNNGG	4 cut(s)	495, 942, 962, 2150
BssMI	GATC	9 cut(s)	205, 342, 771, 789, 849, 1042, 1812, 2158, 2478
BssNI	GRCGYC	2 cut(s)	414, 717
BssT1I	CCWWGG	2 cut(s)	962, 2150
Bst2UI	CCWGG	1 cut(s)	1715
Bst4CI	ACNGT	6 cut(s)	595, 742, 1103, 1460, 1997, 2120
Bst6I	CTCTTC	2 cut(s)	146, 1822
BstACI	GRCGYC	2 cut(s)	414, 717
BstAFI	CTTAAG	1 cut(s)	1961
BstAPI	GCANNNNNTGC	6 cut(s)	408, 568, 1232, 1433, 1478, 1610
BstBAI	YACGTR	1 cut(s)	1921
BstC8I	GCNNGC	8 cut(s)	69, 255, 429, 458, 957, 1131, 1470, 1865
BstDEI	CTNAG	7 cut(s)	15, 833, 1062, 1251, 1682, 1791, 2456
BstDSI	CCRYGG	3 cut(s)	495, 962, 2150
BstENI	CCTNNNNNAGG	1 cut(s)	622
BstFNI	CGCG	3 cut(s)	44, 497, 548
BstH2I	RGCGCY	1 cut(s)	428
BstHHI	GCGC	4 cut(s)	259, 427, 550, 591
BstKTI	GATC	9 cut(s)	208, 345, 774, 792, 852, 1045, 1815, 2161, 2481
BstMAI	GTCTC	1 cut(s)	1058
BstMBI	GATC	9 cut(s)	205, 342, 771, 789, 849, 1042, 1812, 2158, 2478
BstNI	CCWGG	1 cut(s)	1715
BstNSI	RCATGY	3 cut(s)	1867, 1878, 2453
BstSCI	CCNGG	4 cut(s)	168, 812, 855, 1713
BstSFI	CTRYAG	3 cut(s)	866, 1868, 2265
BstSLI	GKGCMC	1 cut(s)	961
BstUI	CGCG	3 cut(s)	44, 497, 548
BstV1I	GCAGC	8 cut(s)	172, 542, 562, 634, 852, 1210, 1465, 2108
BstV2I	GAAGAC	1 cut(s)	589
BstXI	CCANNNNNNTGG	2 cut(s)	1141, 1732
BtgI	CCRYGG	3 cut(s)	495, 962, 2150
BtgZI	GCGATG	1 cut(s)	178
BtsI	GCAGTG	1 cut(s)	1487
BtsIMutI	CAGTG	6 cut(s)	747, 987, 1226, 1427, 1487, 2376
BtuMI	TCGCGA	1 cut(s)	44
BveI	ACCTGC	2 cut(s)	1192, 1401
Cac8I	GCNNGC	8 cut(s)	69, 255, 429, 458, 957, 1131, 1470, 1865
CciI	TCATGA	1 cut(s)	1671
CfoI	GCGC	4 cut(s)	259, 427, 550, 591
Cfr10I	RCCGGY	1 cut(s)	253
Cfr13I	GGNCC	6 cut(s)	56, 171, 816, 1384, 1722, 2423
Cfr42I	CCGCGG	1 cut(s)	498
CseI	GACGC	1 cut(s)	422
CsiI	ACCWGGT	1 cut(s)	1713
Csp6I	GTAC	4 cut(s)	202, 604, 1565, 2116
CviQI	GTAC	4 cut(s)	202, 604, 1565, 2116
DdeI	CTNAG	7 cut(s)	15, 833, 1062, 1251, 1682, 1791, 2456
DpnI	GATC	9 cut(s)	207, 344, 773, 791, 851, 1044, 1814, 2160, 2480
DpnII	GATC	9 cut(s)	205, 342, 771, 789, 849, 1042, 1812, 2158, 2478
EaeI	YGGCCR	7 cut(s)	3, 311, 458, 810, 915, 939, 1363
Eam1104I	CTCTTC	2 cut(s)	146, 1822
EarI	CTCTTC	2 cut(s)	146, 1822
EciI	GGCGGA	3 cut(s)	268, 293, 2357
Ecl136II	GAGCTC	3 cut(s)	178, 466, 615
Eco130I	CCWWGG	2 cut(s)	962, 2150
Eco24I	GRGCYC	3 cut(s)	180, 468, 617
Eco32I	GATATC	2 cut(s)	1522, 2418
Eco47I	GGWCC	3 cut(s)	816, 1722, 2423
Eco53kI	GAGCTC	3 cut(s)	178, 466, 615
Eco57I	CTGAAG	7 cut(s)	503, 714, 897, 1467, 1931, 2037, 2258
EcoICRI	GAGCTC	3 cut(s)	178, 466, 615
EcoNI	CCTNNNNNAGG	1 cut(s)	622
EcoO109I	RGGNCCY	1 cut(s)	816
EcoRI	GAATTC	1 cut(s)	976
EcoRII	CCWGG	1 cut(s)	1713
EcoRV	GATATC	2 cut(s)	1522, 2418
EcoT14I	CCWWGG	2 cut(s)	962, 2150
EcoT38I	GRGCYC	3 cut(s)	180, 468, 617
ErhI	CCWWGG	2 cut(s)	962, 2150
FaqI	GGGAC	2 cut(s)	829, 916
FauI	CCCGC	2 cut(s)	72, 1075
FbaI	TGATCA	1 cut(s)	2158
FblI	GTMKAC	2 cut(s)	11, 1373
FriOI	GRGCYC	3 cut(s)	180, 468, 617
FspBI	CTAG	4 cut(s)	1515, 2073, 2078, 2488
FspI	TGCGCA	1 cut(s)	590
GlaI	GCGC	4 cut(s)	258, 426, 549, 590
GsaI	CCCAGC	1 cut(s)	2336
GsuI	CTGGAG	1 cut(s)	5
HaeII	RGCGCY	1 cut(s)	428
HapII	CCGG	9 cut(s)	169, 254, 435, 446, 461, 813, 856, 948, 1640
HgaI	GACGC	1 cut(s)	422
HhaI	GCGC	4 cut(s)	259, 427, 550, 591
Hin1I	GRCGYC	2 cut(s)	414, 717
Hin6I	GCGC	4 cut(s)	257, 425, 548, 589
HinP1I	GCGC	4 cut(s)	257, 425, 548, 589
HincII	GTYRAC	2 cut(s)	12, 1882
HindII	GTYRAC	2 cut(s)	12, 1882
HpaII	CCGG	9 cut(s)	169, 254, 435, 446, 461, 813, 856, 948, 1640
HphI	GGTGA	2 cut(s)	227, 1603
Hpy166II	GTNNAC	4 cut(s)	12, 959, 1374, 1882
Hpy8I	GTNNAC	4 cut(s)	12, 959, 1374, 1882
Hpy99I	CGWCG	5 cut(s)	49, 416, 719, 829, 1464
HpyAV	CCTTC	2 cut(s)	760, 1168
HpyCH4III	ACNGT	6 cut(s)	595, 742, 1103, 1460, 1997, 2120
HpyCH4IV	ACGT	3 cut(s)	717, 827, 1920
HpyF3I	CTNAG	7 cut(s)	15, 833, 1062, 1251, 1682, 1791, 2456
HpySE526I	ACGT	3 cut(s)	717, 827, 1920
Hsp92I	GRCGYC	2 cut(s)	414, 717
HspAI	GCGC	4 cut(s)	257, 425, 548, 589
KflI	GGGWCCC	1 cut(s)	816
Kpn2I	TCCGGA	1 cut(s)	1639
KroI	GCCGGC	1 cut(s)	253
KroNI	GCCGGC	1 cut(s)	255
Ksp22I	TGATCA	1 cut(s)	2158
KspI	CCGCGG	1 cut(s)	498
Kzo9I	GATC	9 cut(s)	205, 342, 771, 789, 849, 1042, 1812, 2158, 2478
LmnI	GCTCC	5 cut(s)	24, 463, 620, 1285, 1642
Lsp1109I	GCAGC	8 cut(s)	172, 542, 562, 634, 852, 1210, 1465, 2108
LweI	GCATC	7 cut(s)	155, 494, 1628, 1690, 1777, 2357, 2383
MabI	ACCWGGT	1 cut(s)	1713
MaeI	CTAG	4 cut(s)	1515, 2073, 2078, 2488
MaeII	ACGT	3 cut(s)	717, 827, 1920
MaeIII	GTNAC	3 cut(s)	1179, 2102, 2445
MalI	GATC	9 cut(s)	207, 344, 773, 791, 851, 1044, 1814, 2160, 2480
MbiI	CCGCTC	1 cut(s)	67
MboI	GATC	9 cut(s)	205, 342, 771, 789, 849, 1042, 1812, 2158, 2478
MboII	GAAGA	8 cut(s)	133, 589, 707, 1222, 1809, 2030, 2150, 2320
MfeI	CAATTG	1 cut(s)	1556
MhlI	GDGCHC	4 cut(s)	180, 468, 617, 961
MlsI	TGGCCA	3 cut(s)	313, 917, 1365
MluNI	TGGCCA	3 cut(s)	313, 917, 1365
MlyI	GAGTC	4 cut(s)	7, 17, 206, 257
MmeI	TCCRAC	3 cut(s)	848, 1677, 1744
Mox20I	TGGCCA	3 cut(s)	313, 917, 1365
MreI	CGCCGGCG	1 cut(s)	253
MroI	TCCGGA	1 cut(s)	1639
MroNI	GCCGGC	1 cut(s)	253
MroXI	GAANNNNTTC	1 cut(s)	2145
MscI	TGGCCA	3 cut(s)	313, 917, 1365
MseI	TTAA	3 cut(s)	639, 1353, 1962
MslI	CAYNNNNRTG	2 cut(s)	1013, 1805
Msp20I	TGGCCA	3 cut(s)	313, 917, 1365
MspA1I	CMGCKG	3 cut(s)	497, 553, 2222
MspCI	CTTAAG	1 cut(s)	1961
MspI	CCGG	9 cut(s)	169, 254, 435, 446, 461, 813, 856, 948, 1640
MspR9I	CCNGG	4 cut(s)	170, 814, 857, 1715
MunI	CAATTG	1 cut(s)	1556
Mva1269I	GAATGC	2 cut(s)	1438, 2411
MvaI	CCWGG	1 cut(s)	1715
MvnI	CGCG	3 cut(s)	44, 497, 548
NaeI	GCCGGC	1 cut(s)	255
NciI	CCSGG	3 cut(s)	170, 814, 857
NcoI	CCATGG	2 cut(s)	962, 2150
NdeII	GATC	9 cut(s)	205, 342, 771, 789, 849, 1042, 1812, 2158, 2478
NgoMIV	GCCGGC	1 cut(s)	253
NlaIV	GGNNCC	5 cut(s)	58, 817, 818, 1386, 2361
NmeAIII	GCCGAG	3 cut(s)	31, 199, 967
NmuCI	GTSAC	1 cut(s)	2445
NruI	TCGCGA	1 cut(s)	44
NsbI	TGCGCA	1 cut(s)	590
NspI	RCATGY	3 cut(s)	1867, 1878, 2453
PaeI	GCATGC	1 cut(s)	1867
PagI	TCATGA	1 cut(s)	1671
PciI	ACATGT	1 cut(s)	1874
PcsI	WCGNNNNNNNCGW	1 cut(s)	509
PctI	GAATGC	2 cut(s)	1438, 2411
PdiI	GCCGGC	1 cut(s)	255
PdmI	GAANNNNTTC	1 cut(s)	2145
PfeI	GAWTC	7 cut(s)	49, 438, 500, 524, 1675, 2012, 2064
PflMI	CCANNNNNTGG	1 cut(s)	1206
PleI	GAGTC	4 cut(s)	7, 16, 206, 257
PpsI	GAGTC	4 cut(s)	7, 16, 206, 257
Ppu21I	YACGTR	1 cut(s)	1921
PpuMI	RGGWCCY	1 cut(s)	816
PscI	ACATGT	1 cut(s)	1874
PshBI	ATTAAT	1 cut(s)	1353
Psp124BI	GAGCTC	3 cut(s)	180, 468, 617
Psp5II	RGGWCCY	1 cut(s)	816
Psp6I	CCWGG	1 cut(s)	1713
PspFI	CCCAGC	1 cut(s)	2332
PspGI	CCWGG	1 cut(s)	1713
PspN4I	GGNNCC	5 cut(s)	58, 817, 818, 1386, 2361
PspPI	GGNCC	6 cut(s)	56, 171, 816, 1384, 1722, 2423
PspPPI	RGGWCCY	1 cut(s)	816
PstI	CTGCAG	1 cut(s)	870
PvuII	CAGCTG	2 cut(s)	553, 2222
RruI	TCGCGA	1 cut(s)	44
RsaI	GTAC	4 cut(s)	203, 605, 1566, 2117
RsaNI	GTAC	4 cut(s)	202, 604, 1565, 2116
RseI	CAYNNNNRTG	2 cut(s)	1013, 1805
SacI	GAGCTC	3 cut(s)	180, 468, 617
SacII	CCGCGG	1 cut(s)	498
SalI	GTCGAC	1 cut(s)	10
SaqAI	TTAA	3 cut(s)	639, 1353, 1962
Sau3AI	GATC	9 cut(s)	205, 342, 771, 789, 849, 1042, 1812, 2158, 2478
Sau96I	GGNCC	6 cut(s)	56, 171, 816, 1384, 1722, 2423
SchI	GAGTC	4 cut(s)	7, 17, 206, 257
ScrFI	CCNGG	4 cut(s)	170, 814, 857, 1715
SduI	GDGCHC	4 cut(s)	180, 468, 617, 961
SexAI	ACCWGGT	1 cut(s)	1713
SfaNI	GCATC	7 cut(s)	155, 494, 1628, 1690, 1777, 2357, 2383
SfcI	CTRYAG	3 cut(s)	866, 1868, 2265
Sfr303I	CCGCGG	1 cut(s)	498
SgrAI	CRCCGGYG	1 cut(s)	253
SgrBI	CCGCGG	1 cut(s)	498
SinI	GGWCC	3 cut(s)	816, 1722, 2423
SmiMI	CAYNNNNRTG	2 cut(s)	1013, 1805
SmlI	CTYRAG	4 cut(s)	32, 610, 1961, 2283
SmoI	CTYRAG	4 cut(s)	32, 610, 1961, 2283
SpeI	ACTAGT	1 cut(s)	2072
SphI	GCATGC	1 cut(s)	1867
SspI	AATATT	1 cut(s)	2388
SspMI	CTAG	4 cut(s)	1515, 2073, 2078, 2488
SstI	GAGCTC	3 cut(s)	180, 468, 617
StyD4I	CCNGG	4 cut(s)	168, 812, 855, 1713
StyI	CCWWGG	2 cut(s)	962, 2150
TaaI	ACNGT	6 cut(s)	595, 742, 1103, 1460, 1997, 2120
TaiI	ACGT	3 cut(s)	720, 830, 1923
TaqI	TCGA	3 cut(s)	11, 2431, 2461
TatI	WGTACW	2 cut(s)	603, 1564
TauI	GCSGC	4 cut(s)	434, 511, 562, 840
TfiI	GAWTC	7 cut(s)	49, 438, 500, 524, 1675, 2012, 2064
Tru1I	TTAA	3 cut(s)	639, 1353, 1962
Tru9I	TTAA	3 cut(s)	639, 1353, 1962
TscAI	CASTG	6 cut(s)	747, 987, 1233, 1434, 1494, 2383
TseFI	GTSAC	1 cut(s)	2445
TseI	GCWGC	8 cut(s)	160, 530, 550, 647, 865, 1223, 1478, 2096
Tsp45I	GTSAC	1 cut(s)	2445
TspGWI	ACGGA	1 cut(s)	340
TspRI	CASTG	6 cut(s)	747, 987, 1233, 1434, 1494, 2383
Van91I	CCANNNNNTGG	1 cut(s)	1206
Vha464I	CTTAAG	1 cut(s)	1961
VneI	GTGCAC	1 cut(s)	957
VpaK11BI	GGWCC	3 cut(s)	816, 1722, 2423
VspI	ATTAAT	1 cut(s)	1353
XagI	CCTNNNNNAGG	1 cut(s)	622
XapI	RAATTY	4 cut(s)	976, 1245, 1496, 1775
XceI	RCATGY	3 cut(s)	1867, 1878, 2453
XcmI	CCANNNNNNNNNTGG	2 cut(s)	889, 1711
XmiI	GTMKAC	2 cut(s)	11, 1373
XmnI	GAANNNNTTC	1 cut(s)	2145
XspI	CTAG	4 cut(s)	1515, 2073, 2078, 2488
ZraI	GACGTC	1 cut(s)	718

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.