Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0004551

Physical Location & Seq

Genomic Coordinates Reverse (-)

58659037 .. 58661417

Gene Structure 2381 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG354700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1512 bp

ATGGCGGAGTCGACTGAGCATCAGCAACTGGCGGCCATTCTCGGTCCGGAGCCGGCCGCCACTTTCCAAACCGTAATCTCCCACTTAATCTCCTCCCCCTCCTCTTTGCAAACCCTAATCTCCGACCTCATCTCCTCCGAGAAGCGGTCCGAGGCCGAGCTCGTCTTCAACATCTGCAAGCAGGCCGATCACGAGTCGCTTTCTCTCAAACTCGCTCAGCTTCTCCAGGTCTCTCCCGACGAGAAGACCCGAGCCATGTGCTCCATTCTCCTCCGGAAGCAACTACTCACCCGCGACCTGTGGCGAAGACTCTCTCGTAACACCAAATCCACCCTCAAACATACTTTATTATCTTGTATTCAACGCGAGGACACCCCAACATCGATTTCGAAGGCCATCTCCTACGCCGTGTCGGAGCTCGCCTCCGCAGTGTTGTTCGCCGAAGGCTGGCCGGAGCTCTTGCCCTTTCAGTTCCAATCCACCTCCTACGATGCGCCGCCGAAGCTTCAGGAATGCTCCTTCTTGATTTTCGATCAGCTCGCGGACTTCTTTACCCCGTTTATAGACGAATTACACACCGTCTTCTTATACTGCTTGAGCTCCTCCACAAGGTCAGATGAAGTTAAGATAGCCGCGTTTAGTGCGGTGGTCAGTTTCATTCGGTGTTTGTCCAGCTCTGAAGATCCGAATATGTTTCACGACGTCTTGATAGAAATGATGAGAACAGTGATGGGGGAATTGAACAAAGGGAAGGAGATCATGGCACAGGAGGCGATCAAGTTGTTCATTGAATTGGCCGGGACCCATCCGACGTTGCTCAGCCGCAACGGGCAGATCATCAGGGCTATGCTGCAGATTGCTGAAGCCATAGATGTCTTGGAACAAGGGACGAGGCACTTGGCCATTGAGTTTCTGATTACTCTGGCCGAGGCCGGAGAGCGTGCACCATGGATAATGAGGAGGTGTCGGGAGTTTGTTGGTCTACTTTTTCCCATTTTAATGAGGATGGTTTCAAATGTCAAGGATGATCCCTCATGCCATACTGCTGAGACTGATGATGACATTGCCTTGGGTGCAAGTGGTGATTACTGTGTTGGGCAGGAGTGTTTGCATAGGCTTGCCATTGCCTTGGGTGGCAATAACATTGTTCCAATTGCGTTAGAACACTTTACTTCCCATTTTGCTGCACCCGAGTGGCCCAAGCATCATGCAGCATTGATTGCCCTTGCTCAAATCGCTGAGGGTTGCTCTGAGGTAATGATAAAAGATTTGGAGCAAGTGGTGGCAATGATTTTGAACTCCCTTGAACATCCACATATTCGGGTAAGGTGGTCAGCTATTAATGCAGTTGGCCAGTTGTCTACTTATTTGGCCCCAGACCTGCAAGTTCAATATCATCAACAGGTGTTTTCTGCACTGAATGCCACTATATATGGTTTGCAGAACCGTCATCTACTGGCACATGCTGCTTCAGCACTGCAAAAATTCATTGAGAAATGTGCTAGGGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000060 GO:0001101 GO:0003674 GO:0005048 GO:0005215 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008139 GO:0008150 GO:0008565 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0017038 GO:0031090 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032386 GO:0032388 GO:0032879 GO:0032880 GO:0033036 GO:0033157 GO:0033218 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0042221 GO:0042277 GO:0042306 GO:0042307 GO:0042886 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046822 GO:0046824 GO:0046907 GO:0048518 GO:0050789 GO:0050896 GO:0051049 GO:0051050 GO:0051169 GO:0051170 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0060341 GO:0065007 GO:0070013 GO:0070201 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071702 GO:0071705 GO:0072594 GO:0090087 GO:0090316 GO:1900180 GO:1900182 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1903827 GO:1903829 GO:1904589 GO:1904591 GO:1904951

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HEAT HEAT_2 HEAT_EZ

Protein Analysis

503

Amino Acids

56.0

Weight (kDa)

5.63

Isoelectric Point (pI)

49.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_IPO5	PF25780	128 - 275	8.5e-16	IPO5-like, TPR repeats
Importin_rep_4	PF18808	293 - 384	3.3e-13	Importin repeat
HEAT_EZ	PF13513	399 - 453	3.1e-07	HEAT-like repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004551)

Species	Orthologous Gene IDs
malus_domestica	MD11G1005500.v1.1
rosa_chinensis	RchiOBHm_Chr5g0054081 RchiOBHm_Chr5g0054171 RchiOBHm_Chr5g0054201 RchiOBHm_Chr5g0054251
rosa_laevigata	RLG00000034915 RLG00000034916 RLG00000034918 RLG00000034920 RLG00000034921
rosa_multiflora	Rmu_sc0000094.1_g000006 Rmu_sc0000509.1_g000025 Rmu_sc0006889.1_g000007 Rmu_sc0010082.1_g000001 Rmu_sc0023670.1_g000001
rosa_roxburghii	Rroxscaffold_1G00026650 Rroxscaffold_1G00026670
rosa_rugosa	Rorug05G0285300
rosa_samantha	Rh5AG354200 Rh5AG354300 Rh5AG354700 Rh5AG355200 Rh5BG366000 Rh5BG366100 Rh5BG367300 Rh5CG390800 Rh5DG055000 Rh5DG380900 Rh5DG381500
rosa_wichuraiana	Rw5G033390 Rw5G033410 Rw5G033590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	705
Acc36I	ACCTGC	1 cut(s)	1389
AccI	GTMKAC	3 cut(s)	11, 982, 1361
AccII	CGCG	4 cut(s)	294, 366, 542, 635
AccIII	TCCGGA	2 cut(s)	46, 273
AclWI	GGATC	2 cut(s)	677, 1022
AcoI	YGGCCR	7 cut(s)	33, 54, 449, 795, 900, 924, 1351
AcsI	RAATTY	1 cut(s)	1484
AcuI	CTGAAG	4 cut(s)	491, 699, 882, 1455
AcyI	GRCGYC	1 cut(s)	702
AfiI	CCNNNNNNNGG	3 cut(s)	144, 447, 609
AgsI	TTSAA	8 cut(s)	169, 362, 742, 791, 1014, 1297, 1307, 1391
AjnI	CCWGG	1 cut(s)	225
AjuI	GAANNNNNNNTTGG	2 cut(s)	370, 402
AleI	CACNNNNGTG	1 cut(s)	1192
AluBI	AGCT	9 cut(s)	160, 220, 418, 457, 505, 538, 600, 675, 1337
AluI	AGCT	9 cut(s)	160, 220, 418, 457, 505, 538, 600, 675, 1337
Alw21I	GWGCWC	6 cut(s)	162, 263, 420, 459, 602, 946
Alw26I	GTCTC	2 cut(s)	235, 1043
Alw44I	GTGCAC	1 cut(s)	942
AlwI	GGATC	2 cut(s)	677, 1022
AlwNI	CAGNNNCTG	1 cut(s)	28
Ama87I	CYCGRG	2 cut(s)	249, 1190
Aor13HI	TCCGGA	2 cut(s)	46, 273
ApaLI	GTGCAC	1 cut(s)	942
ApeKI	GCWGC	4 cut(s)	850, 1184, 1211, 1466
ApoI	RAATTY	1 cut(s)	1484
AseI	ATTAAT	1 cut(s)	1341
AspLEI	GCGC	1 cut(s)	496
AspS9I	GGNCC	5 cut(s)	44, 147, 801, 1197, 1372
AsuC2I	CCSGG	1 cut(s)	799
AsuHPI	GGTGA	2 cut(s)	280, 1094
AsuII	TTCGAA	1 cut(s)	389
AvaI	CYCGRG	2 cut(s)	249, 1190
AvaII	GGWCC	3 cut(s)	44, 147, 801
BaeGI	GKGCMC	1 cut(s)	946
BalI	TGGCCA	2 cut(s)	902, 1353
BanII	GRGCYC	4 cut(s)	162, 420, 459, 602
BauI	CACGAG	1 cut(s)	191
BbsI	GAAGAC	4 cut(s)	157, 251, 313, 574
Bbv12I	GWGCWC	6 cut(s)	162, 263, 420, 459, 602, 946
BbvCI	CCTCAGC	1 cut(s)	1239
BbvI	GCAGC	4 cut(s)	837, 1171, 1223, 1453
BccI	CCATC	4 cut(s)	404, 724, 813, 1000
BceAI	ACGGC	1 cut(s)	392
BcgI	CGANNNNNNTGC	2 cut(s)	240, 274
BciT130I	CCWGG	1 cut(s)	227
BcnI	CCSGG	1 cut(s)	799
BcoDI	GTCTC	2 cut(s)	235, 1043
BfaI	CTAG	1 cut(s)	1503
BfmI	CTRYAG	1 cut(s)	851
BfuAI	ACCTGC	1 cut(s)	1389
BisI	GCNGC	9 cut(s)	33, 57, 497, 633, 823, 851, 1185, 1212, 1467
BlpI	GCTNAGC	2 cut(s)	216, 818
BlsI	GCNGC	9 cut(s)	34, 58, 498, 634, 824, 852, 1186, 1213, 1468
Bme1390I	CCNGG	2 cut(s)	227, 799
Bme18I	GGWCC	3 cut(s)	44, 147, 801
BmeT110I	CYCGRG	2 cut(s)	249, 1190
BmgT120I	GGNCC	5 cut(s)	44, 147, 801, 1197, 1372
BmiI	GGNNCC	4 cut(s)	51, 802, 803, 1374
BmrFI	CCNGG	2 cut(s)	227, 799
BmsI	GCATC	3 cut(s)	28, 481, 1213
BpiI	GAAGAC	4 cut(s)	157, 251, 313, 574
BplI	GAGNNNNNCTC	4 cut(s)	407, 439, 1232, 1264
BpmI	CTGGAG	1 cut(s)	209
Bpu10I	CCTNAGC	1 cut(s)	1239
Bpu1102I	GCTNAGC	2 cut(s)	216, 818
Bpu14I	TTCGAA	1 cut(s)	389
BpuEI	CTTGAG	1 cut(s)	616
BpuMI	CCSGG	1 cut(s)	799
Bsa29I	ATCGAT	1 cut(s)	383
BsaHI	GRCGYC	1 cut(s)	702
BsaI	GGTCTC	1 cut(s)	235
BsaJI	CCNNGG	5 cut(s)	150, 927, 947, 1068, 1128
BsaWI	WCCGGW	2 cut(s)	46, 273
BsaXI	ACNNNNNCTCC	4 cut(s)	74, 104, 116, 146
Bsc4I	CCNNNNNNNGG	3 cut(s)	144, 447, 609
Bse118I	RCCGGY	1 cut(s)	52
Bse1I	ACTGG	3 cut(s)	33, 1354, 1461
Bse3DI	GCAATG	3 cut(s)	1062, 1122, 1293
BseAI	TCCGGA	2 cut(s)	46, 273
BseBI	CCWGG	1 cut(s)	227
BseCI	ATCGAT	1 cut(s)	383
BseDI	CCNNGG	5 cut(s)	150, 927, 947, 1068, 1128
BseGI	GGATG	4 cut(s)	805, 1011, 1030, 1309
BseLI	CCNNNNNNNGG	3 cut(s)	144, 447, 609
BseMI	GCAATG	3 cut(s)	1062, 1122, 1293
BseMII	CTCAG	6 cut(s)	6, 230, 832, 1038, 1230, 1242
BseNI	ACTGG	3 cut(s)	33, 1354, 1461
BseRI	GAGGAG	6 cut(s)	82, 91, 124, 260, 592, 973
BseSI	GKGCMC	1 cut(s)	946
BseX3I	CGGCCG	1 cut(s)	54
BseXI	GCAGC	4 cut(s)	837, 1171, 1223, 1453
BsgI	GTGCAG	2 cut(s)	1170, 1398
Bsh1236I	CGCG	4 cut(s)	294, 366, 542, 635
Bsh1285I	CGRYCG	1 cut(s)	57
BshVI	ATCGAT	1 cut(s)	383
BsiEI	CGRYCG	1 cut(s)	57
BsiHKAI	GWGCWC	6 cut(s)	162, 263, 420, 459, 602, 946
BsiHKCI	CYCGRG	2 cut(s)	249, 1190
BsiSI	CCGG	6 cut(s)	47, 53, 274, 452, 798, 933
BslFI	GGGAC	2 cut(s)	814, 901
BslI	CCNNNNNNNGG	3 cut(s)	144, 447, 609
BsmAI	GTCTC	2 cut(s)	235, 1043
BsmFI	GGGAC	2 cut(s)	814, 901
BsmI	GAATGC	2 cut(s)	518, 1426
Bso31I	GGTCTC	1 cut(s)	235
BsoBI	CYCGRG	2 cut(s)	249, 1190
Bsp119I	TTCGAA	1 cut(s)	389
Bsp1286I	GDGCHC	6 cut(s)	162, 263, 420, 459, 602, 946
Bsp13I	TCCGGA	2 cut(s)	46, 273
Bsp143I	GATC	7 cut(s)	187, 532, 682, 756, 774, 834, 1027
Bsp1720I	GCTNAGC	2 cut(s)	216, 818
Bsp19I	CCATGG	1 cut(s)	947
BspCNI	CTCAG	6 cut(s)	7, 229, 831, 1039, 1231, 1243
BspDI	ATCGAT	1 cut(s)	383
BspEI	TCCGGA	2 cut(s)	46, 273
BspFNI	CGCG	4 cut(s)	294, 366, 542, 635
BspLI	GGNNCC	4 cut(s)	51, 802, 803, 1374
BspMAI	CTGCAG	1 cut(s)	855
BspMI	ACCTGC	1 cut(s)	1389
BspPI	GGATC	2 cut(s)	677, 1022
BspT104I	TTCGAA	1 cut(s)	389
BspTNI	GGTCTC	1 cut(s)	235
BsrDI	GCAATG	3 cut(s)	1062, 1122, 1293
BsrFI	RCCGGY	1 cut(s)	52
BsrI	ACTGG	3 cut(s)	33, 1354, 1461
BssAI	RCCGGY	1 cut(s)	52
BssECI	CCNNGG	5 cut(s)	150, 927, 947, 1068, 1128
BssMI	GATC	7 cut(s)	187, 532, 682, 756, 774, 834, 1027
BssNI	GRCGYC	1 cut(s)	702
BssSI	CACGAG	1 cut(s)	191
BssT1I	CCWWGG	3 cut(s)	947, 1068, 1128
Bst2BI	CACGAG	1 cut(s)	191
Bst2UI	CCWGG	1 cut(s)	227
Bst4CI	ACNGT	5 cut(s)	73, 580, 727, 1091, 1448
BstACI	GRCGYC	1 cut(s)	702
BstAPI	GCANNNNNTGC	3 cut(s)	1220, 1421, 1466
BstBI	TTCGAA	1 cut(s)	389
BstC8I	GCNNGC	8 cut(s)	54, 179, 183, 420, 449, 540, 942, 1119
BstDEI	CTNAG	6 cut(s)	15, 216, 818, 1047, 1239, 1251
BstDSI	CCRYGG	1 cut(s)	947
BstENI	CCTNNNNNAGG	1 cut(s)	607
BstF5I	GGATG	4 cut(s)	805, 1011, 1030, 1309
BstFNI	CGCG	4 cut(s)	294, 366, 542, 635
BstHHI	GCGC	1 cut(s)	496
BstKTI	GATC	7 cut(s)	190, 535, 685, 759, 777, 837, 1030
BstMAI	GTCTC	2 cut(s)	235, 1043
BstMBI	GATC	7 cut(s)	187, 532, 682, 756, 774, 834, 1027
BstMCI	CGRYCG	1 cut(s)	57
BstNI	CCWGG	1 cut(s)	227
BstNSI	RCATGY	1 cut(s)	1466
BstSCI	CCNGG	2 cut(s)	225, 797
BstSFI	CTRYAG	1 cut(s)	851
BstSLI	GKGCMC	1 cut(s)	946
BstUI	CGCG	4 cut(s)	294, 366, 542, 635
BstV1I	GCAGC	4 cut(s)	837, 1171, 1223, 1453
BstV2I	GAAGAC	4 cut(s)	157, 251, 313, 574
BstX2I	RGATCY	1 cut(s)	682
BstXI	CCANNNNNNTGG	1 cut(s)	1129
BstYI	RGATCY	1 cut(s)	682
BstZI	CGGCCG	1 cut(s)	54
Bsu15I	ATCGAT	1 cut(s)	383
BsuTUI	ATCGAT	1 cut(s)	383
BtgI	CCRYGG	1 cut(s)	947
BtsCI	GGATG	4 cut(s)	805, 1011, 1030, 1309
BtsI	GCAGTG	2 cut(s)	435, 1475
BtsIMutI	CAGTG	4 cut(s)	435, 732, 1415, 1475
BveI	ACCTGC	1 cut(s)	1389
Cac8I	GCNNGC	8 cut(s)	54, 179, 183, 420, 449, 540, 942, 1119
CaiI	CAGNNNCTG	1 cut(s)	28
CfoI	GCGC	1 cut(s)	496
Cfr10I	RCCGGY	1 cut(s)	52
Cfr13I	GGNCC	5 cut(s)	44, 147, 801, 1197, 1372
ClaI	ATCGAT	1 cut(s)	383
CpoI	CGGWCCG	2 cut(s)	44, 147
CspI	CGGWCCG	2 cut(s)	44, 147
CviAII	CATG	6 cut(s)	256, 760, 948, 1035, 1208, 1463
DdeI	CTNAG	6 cut(s)	15, 216, 818, 1047, 1239, 1251
DpnI	GATC	7 cut(s)	189, 534, 684, 758, 776, 836, 1029
DpnII	GATC	7 cut(s)	187, 532, 682, 756, 774, 834, 1027
EaeI	YGGCCR	7 cut(s)	33, 54, 449, 795, 900, 924, 1351
EagI	CGGCCG	1 cut(s)	54
EciI	GGCGGA	1 cut(s)	20
Ecl136II	GAGCTC	4 cut(s)	160, 418, 457, 600
EclXI	CGGCCG	1 cut(s)	54
Eco130I	CCWWGG	3 cut(s)	947, 1068, 1128
Eco24I	GRGCYC	4 cut(s)	162, 420, 459, 602
Eco31I	GGTCTC	1 cut(s)	235
Eco47I	GGWCC	3 cut(s)	44, 147, 801
Eco52I	CGGCCG	1 cut(s)	54
Eco53kI	GAGCTC	4 cut(s)	160, 418, 457, 600
Eco57I	CTGAAG	4 cut(s)	491, 699, 882, 1455
Eco88I	CYCGRG	2 cut(s)	249, 1190
EcoICRI	GAGCTC	4 cut(s)	160, 418, 457, 600
EcoNI	CCTNNNNNAGG	1 cut(s)	607
EcoO109I	RGGNCCY	1 cut(s)	801
EcoRII	CCWGG	1 cut(s)	225
EcoT14I	CCWWGG	3 cut(s)	947, 1068, 1128
EcoT38I	GRGCYC	4 cut(s)	162, 420, 459, 602
ErhI	CCWWGG	3 cut(s)	947, 1068, 1128
FaeI	CATG	6 cut(s)	259, 763, 951, 1038, 1211, 1466
FaqI	GGGAC	2 cut(s)	814, 901
FatI	CATG	6 cut(s)	255, 759, 947, 1034, 1207, 1462
FauI	CCCGC	1 cut(s)	299
FblI	GTMKAC	3 cut(s)	11, 982, 1361
Fnu4HI	GCNGC	9 cut(s)	33, 57, 497, 633, 823, 851, 1185, 1212, 1467
FokI	GGATG	4 cut(s)	792, 1018, 1037, 1296
FriOI	GRGCYC	4 cut(s)	162, 420, 459, 602
Fsp4HI	GCNGC	9 cut(s)	33, 57, 497, 633, 823, 851, 1185, 1212, 1467
FspBI	CTAG	1 cut(s)	1503
GlaI	GCGC	1 cut(s)	495
GluI	GCNGC	9 cut(s)	33, 57, 497, 633, 823, 851, 1185, 1212, 1467
GsuI	CTGGAG	1 cut(s)	209
HapII	CCGG	6 cut(s)	47, 53, 274, 452, 798, 933
HhaI	GCGC	1 cut(s)	496
Hin1I	GRCGYC	1 cut(s)	702
Hin1II	CATG	6 cut(s)	259, 763, 951, 1038, 1211, 1466
Hin6I	GCGC	1 cut(s)	494
HinP1I	GCGC	1 cut(s)	494
HincII	GTYRAC	1 cut(s)	12
HindII	GTYRAC	1 cut(s)	12
HindIII	AAGCTT	1 cut(s)	503
HinfI	GANTC	3 cut(s)	8, 194, 309
HpaII	CCGG	6 cut(s)	47, 53, 274, 452, 798, 933
HphI	GGTGA	2 cut(s)	280, 1094
Hpy166II	GTNNAC	4 cut(s)	12, 944, 983, 1362
Hpy188III	TCNNGA	9 cut(s)	47, 191, 236, 274, 509, 523, 698, 706, 968
Hpy8I	GTNNAC	4 cut(s)	12, 944, 983, 1362
Hpy99I	CGWCG	3 cut(s)	242, 704, 814
HpyAV	CCTTC	4 cut(s)	385, 437, 529, 745
HpyCH4III	ACNGT	5 cut(s)	73, 580, 727, 1091, 1448
HpyCH4IV	ACGT	2 cut(s)	702, 812
HpyF3I	CTNAG	6 cut(s)	15, 216, 818, 1047, 1239, 1251
HpySE526I	ACGT	2 cut(s)	702, 812
Hsp92I	GRCGYC	1 cut(s)	702
Hsp92II	CATG	6 cut(s)	259, 763, 951, 1038, 1211, 1466
HspAI	GCGC	1 cut(s)	494
KflI	GGGWCCC	1 cut(s)	801
Kpn2I	TCCGGA	2 cut(s)	46, 273
KroI	GCCGGC	1 cut(s)	52
KroNI	GCCGGC	1 cut(s)	54
Kzo9I	GATC	7 cut(s)	187, 532, 682, 756, 774, 834, 1027
LmnI	GCTCC	7 cut(s)	49, 266, 415, 454, 521, 605, 1273
Lsp1109I	GCAGC	4 cut(s)	837, 1171, 1223, 1453
LweI	GCATC	3 cut(s)	28, 481, 1213
MaeI	CTAG	1 cut(s)	1503
MaeII	ACGT	2 cut(s)	702, 812
MaeIII	GTNAC	1 cut(s)	317
MalI	GATC	7 cut(s)	189, 534, 684, 758, 776, 836, 1029
MboI	GATC	7 cut(s)	187, 532, 682, 756, 774, 834, 1027
MboII	GAAGA	5 cut(s)	157, 256, 318, 574, 692
MfeI	CAATTG	1 cut(s)	1152
MflI	RGATCY	1 cut(s)	682
MhlI	GDGCHC	6 cut(s)	162, 263, 420, 459, 602, 946
MlsI	TGGCCA	2 cut(s)	902, 1353
MluCI	AATT	5 cut(s)	569, 737, 791, 1152, 1484
MluNI	TGGCCA	2 cut(s)	902, 1353
MlyI	GAGTC	3 cut(s)	17, 203, 303
MmeI	TCCRAC	3 cut(s)	147, 393, 833
Mox20I	TGGCCA	2 cut(s)	902, 1353
MroI	TCCGGA	2 cut(s)	46, 273
MroNI	GCCGGC	1 cut(s)	52
MscI	TGGCCA	2 cut(s)	902, 1353
MseI	TTAA	4 cut(s)	86, 624, 998, 1341
MslI	CAYNNNNRTG	2 cut(s)	998, 1192
Msp20I	TGGCCA	2 cut(s)	902, 1353
MspI	CCGG	6 cut(s)	47, 53, 274, 452, 798, 933
MspR9I	CCNGG	2 cut(s)	227, 799
MunI	CAATTG	1 cut(s)	1152
Mva1269I	GAATGC	2 cut(s)	518, 1426
MvaI	CCWGG	1 cut(s)	227
MvnI	CGCG	4 cut(s)	294, 366, 542, 635
NaeI	GCCGGC	1 cut(s)	54
NciI	CCSGG	1 cut(s)	799
NcoI	CCATGG	1 cut(s)	947
NdeII	GATC	7 cut(s)	187, 532, 682, 756, 774, 834, 1027
NgoMIV	GCCGGC	1 cut(s)	52
NlaIII	CATG	6 cut(s)	259, 763, 951, 1038, 1211, 1466
NlaIV	GGNNCC	4 cut(s)	51, 802, 803, 1374
NmeAIII	GCCGAG	2 cut(s)	181, 952
NspI	RCATGY	1 cut(s)	1466
NspV	TTCGAA	1 cut(s)	389
OliI	CACNNNNGTG	1 cut(s)	1192
PctI	GAATGC	2 cut(s)	518, 1426
PdiI	GCCGGC	1 cut(s)	54
PkrI	GCNGC	9 cut(s)	34, 58, 498, 634, 824, 852, 1186, 1213, 1468
PleI	GAGTC	3 cut(s)	16, 202, 303
PpsI	GAGTC	3 cut(s)	16, 202, 303
PpuMI	RGGWCCY	1 cut(s)	801
PshBI	ATTAAT	1 cut(s)	1341
Psp124BI	GAGCTC	4 cut(s)	162, 420, 459, 602
Psp5II	RGGWCCY	1 cut(s)	801
Psp6I	CCWGG	1 cut(s)	225
PspGI	CCWGG	1 cut(s)	225
PspN4I	GGNNCC	4 cut(s)	51, 802, 803, 1374
PspPI	GGNCC	5 cut(s)	44, 147, 801, 1197, 1372
PspPPI	RGGWCCY	1 cut(s)	801
PstI	CTGCAG	1 cut(s)	855
PstNI	CAGNNNCTG	1 cut(s)	28
PsuI	RGATCY	1 cut(s)	682
RseI	CAYNNNNRTG	2 cut(s)	998, 1192
Rsr2I	CGGWCCG	2 cut(s)	44, 147
RsrII	CGGWCCG	2 cut(s)	44, 147
SacI	GAGCTC	4 cut(s)	162, 420, 459, 602
SalI	GTCGAC	1 cut(s)	10
SaqAI	TTAA	4 cut(s)	86, 624, 998, 1341
SatI	GCNGC	9 cut(s)	33, 57, 497, 633, 823, 851, 1185, 1212, 1467
Sau3AI	GATC	7 cut(s)	187, 532, 682, 756, 774, 834, 1027
Sau96I	GGNCC	5 cut(s)	44, 147, 801, 1197, 1372
SchI	GAGTC	3 cut(s)	17, 203, 303
ScrFI	CCNGG	2 cut(s)	227, 799
SduI	GDGCHC	6 cut(s)	162, 263, 420, 459, 602, 946
SfaNI	GCATC	3 cut(s)	28, 481, 1213
SfcI	CTRYAG	1 cut(s)	851
SfuI	TTCGAA	1 cut(s)	389
SinI	GGWCC	3 cut(s)	44, 147, 801
SmiMI	CAYNNNNRTG	2 cut(s)	998, 1192
SmlI	CTYRAG	1 cut(s)	595
SmoI	CTYRAG	1 cut(s)	595
Sse9I	AATT	5 cut(s)	569, 737, 791, 1152, 1484
SspMI	CTAG	1 cut(s)	1503
SstI	GAGCTC	4 cut(s)	162, 420, 459, 602
StyD4I	CCNGG	2 cut(s)	225, 797
StyI	CCWWGG	3 cut(s)	947, 1068, 1128
TaaI	ACNGT	5 cut(s)	73, 580, 727, 1091, 1448
TaiI	ACGT	2 cut(s)	705, 815
TaqI	TCGA	4 cut(s)	11, 383, 389, 531
TaqII	GACCGA	1 cut(s)	32
TasI	AATT	5 cut(s)	569, 737, 791, 1152, 1484
TauI	GCSGC	5 cut(s)	35, 59, 499, 635, 825
Tru1I	TTAA	4 cut(s)	86, 624, 998, 1341
Tru9I	TTAA	4 cut(s)	86, 624, 998, 1341
TscAI	CASTG	4 cut(s)	435, 732, 1422, 1482
TseI	GCWGC	4 cut(s)	850, 1184, 1211, 1466
TspDTI	ATGAA	4 cut(s)	633, 646, 775, 1477
TspRI	CASTG	4 cut(s)	435, 732, 1422, 1482
VneI	GTGCAC	1 cut(s)	942
VpaK11BI	GGWCC	3 cut(s)	44, 147, 801
VspI	ATTAAT	1 cut(s)	1341
XagI	CCTNNNNNAGG	1 cut(s)	607
XapI	RAATTY	1 cut(s)	1484
XceI	RCATGY	1 cut(s)	1466
XcmI	CCANNNNNNNNNTGG	1 cut(s)	874
XmiI	GTMKAC	3 cut(s)	11, 982, 1361
XspI	CTAG	1 cut(s)	1503
ZraI	GACGTC	1 cut(s)	703

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.