RchiOBHm_Chr5g0066821

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0004466
Physical Location & Seq
Reverse (-)
72909658 .. 72913165
3508 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34257

Sequence Viewer

Length: 813 bp
ATGATACAGCAACAAACGATGAATGGCTCACTTGGTGCACTAAACTCAATTGCTAATCCACCCGATCAGATAAAAACATTAGAAATTGAGTTAGCTAACATAAAGTCTGAGTTACAGCGCGAGTCAATGTTTAGACAGCAAGAACGACAACAATTAGCAGAGGAGCAGGCTCGAGCATCCATCCTTACATCTGAGAAGCAAGAATTGGAAGAAAAACTTGCAGCTTTGTCTAGAAAGTCCTCAGAAGTGTCAGACAAAGTGGCCAGCATGGGATTTTCATTAGAAGACAAGGAGTTACTCGAGAAGCAGATGCATGACATGGCCGTAGTGATTGAAAAATTGGAAAGCAGTAGACAAAAGCTTTTGATGGAGATTGATTCCCAATCTTCGGAGATAGAAAGGCTTTTTGAGGAAAACTCTAATTTATCTAGTTCTCATCAGGAGGCTAAGAGCATAGCTTTGCACTGGGAGAATCAGGTGAAAGACTGTCTTAAGCAAAATGAAGAGCTCCGAGGAATCTTAGATAAGTTAAGAACTGACCAGGCCAAGGGGTTGCTCGACTCCCATAGCAATGGTTCTAACAAGACTGGTTTAGCTGAATACACACCTGAAGTTCTCTCTATTAAGGATCAACTTGCAAAAGAACAGAGCAAAGCAGAGGCATTATCAGCTGAGGTATTACAGCTCTCAGCAAGACTCCAACAAACCACTCAAGCATACTATGGTCTTGCACGCTTCTATAAACCGGTGTTGCGAAACATAGAAAGCAGTCTCATTAAGATGAAACAAGATGGCCCTGTGACAGTGCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

270

Amino Acids

30.49

Weight (kDa)

5.29

Isoelectric Point (pI)

65.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 352
AccII CGCG 1 cut(s) 120
AciI CCGC 1 cut(s) 808
AclWI GGATC 1 cut(s) 636
AcoI YGGCCR 2 cut(s) 261, 321
AcuI CTGAAG 1 cut(s) 630
AdeI CACNNNGTG 1 cut(s) 35
AfiI CCNNNNNNNGG 2 cut(s) 388, 547
AflII CTTAAG 1 cut(s) 491
AgeI ACCGGT 1 cut(s) 745
AgsI TTSAA 1 cut(s) 335
AjnI CCWGG 1 cut(s) 540
AjuI GAANNNNNNNTTGG 2 cut(s) 188, 220
AluBI AGCT 8 cut(s) 95, 224, 361, 458, 508, 596, 671, 685
AluI AGCT 8 cut(s) 95, 224, 361, 458, 508, 596, 671, 685
Alw21I GWGCWC 2 cut(s) 40, 510
Alw26I GTCTC 1 cut(s) 776
Alw44I GTGCAC 1 cut(s) 36
AlwI GGATC 1 cut(s) 636
Ama87I CYCGRG 2 cut(s) 171, 299
AoxI GGCC 4 cut(s) 261, 321, 543, 793
ApaLI GTGCAC 1 cut(s) 36
ApeKI GCWGC 1 cut(s) 221
AsiGI ACCGGT 1 cut(s) 745
AspLEI GCGC 1 cut(s) 120
AspS9I GGNCC 1 cut(s) 794
AsuHPI GGTGA 1 cut(s) 490
AvaI CYCGRG 2 cut(s) 171, 299
BaeGI GKGCMC 1 cut(s) 40
BalI TGGCCA 1 cut(s) 263
BanII GRGCYC 1 cut(s) 510
BbsI GAAGAC 1 cut(s) 291
Bbv12I GWGCWC 2 cut(s) 40, 510
BbvCI CCTCAGC 1 cut(s) 672
BbvI GCAGC 1 cut(s) 233
BccI CCATC 3 cut(s) 188, 361, 785
BceAI ACGGC 1 cut(s) 308
BciT130I CCWGG 1 cut(s) 542
BcoDI GTCTC 1 cut(s) 776
BfaI CTAG 2 cut(s) 231, 429
BfrI CTTAAG 1 cut(s) 491
BisI GCNGC 1 cut(s) 222
BlsI GCNGC 1 cut(s) 223
Bme1390I CCNGG 1 cut(s) 542
BmeT110I CYCGRG 2 cut(s) 171, 299
BmgT120I GGNCC 1 cut(s) 794
BmrFI CCNGG 1 cut(s) 542
BmrI ACTGGG 1 cut(s) 475
BmsI GCATC 2 cut(s) 185, 300
BmuI ACTGGG 1 cut(s) 475
BpiI GAAGAC 1 cut(s) 291
BplI GAGNNNNNCTC 2 cut(s) 401, 433
Bpu10I CCTNAGC 1 cut(s) 672
BpuEI CTTGAG 1 cut(s) 696
BsaJI CCNNGG 2 cut(s) 511, 546
BsaWI WCCGGW 1 cut(s) 745
Bsc4I CCNNNNNNNGG 2 cut(s) 388, 547
Bse118I RCCGGY 1 cut(s) 745
Bse1I ACTGG 2 cut(s) 470, 592
Bse3DI GCAATG 1 cut(s) 577
BseBI CCWGG 1 cut(s) 542
BseDI CCNNGG 2 cut(s) 511, 546
BseGI GGATG 2 cut(s) 176, 180
BseLI CCNNNNNNNGG 2 cut(s) 388, 547
BseMI GCAATG 1 cut(s) 577
BseMII CTCAG 5 cut(s) 99, 183, 255, 663, 702
BseNI ACTGG 2 cut(s) 470, 592
BseRI GAGGAG 1 cut(s) 176
BseSI GKGCMC 1 cut(s) 40
BseXI GCAGC 1 cut(s) 233
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 4 cut(s) 263, 323, 545, 795
BshTI ACCGGT 1 cut(s) 745
BsiHKAI GWGCWC 2 cut(s) 40, 510
BsiHKCI CYCGRG 2 cut(s) 171, 299
BsiSI CCGG 1 cut(s) 746
BslI CCNNNNNNNGG 2 cut(s) 388, 547
BsmAI GTCTC 1 cut(s) 776
BsnI GGCC 4 cut(s) 263, 323, 545, 795
BsoBI CYCGRG 2 cut(s) 171, 299
Bsp1286I GDGCHC 2 cut(s) 40, 510
Bsp143I GATC 2 cut(s) 64, 628
BspACI CCGC 1 cut(s) 808
BspANI GGCC 4 cut(s) 263, 323, 545, 795
BspCNI CTCAG 5 cut(s) 100, 184, 254, 664, 701
BspFNI CGCG 1 cut(s) 120
BspPI GGATC 1 cut(s) 636
BspQI GCTCTTC 1 cut(s) 498
BspTI CTTAAG 1 cut(s) 491
BsrDI GCAATG 1 cut(s) 577
BsrFI RCCGGY 1 cut(s) 745
BsrI ACTGG 2 cut(s) 470, 592
BssAI RCCGGY 1 cut(s) 745
BssECI CCNNGG 2 cut(s) 511, 546
BssMI GATC 2 cut(s) 64, 628
BssT1I CCWWGG 1 cut(s) 546
Bst2UI CCWGG 1 cut(s) 542
Bst4CI ACNGT 2 cut(s) 488, 805
Bst6I CTCTTC 1 cut(s) 498
BstAFI CTTAAG 1 cut(s) 491
BstC8I GCNNGC 3 cut(s) 168, 265, 733
BstDEI CTNAG 7 cut(s) 108, 192, 241, 447, 520, 672, 688
BstF5I GGATG 2 cut(s) 176, 180
BstFNI CGCG 1 cut(s) 120
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 2 cut(s) 67, 631
BstMAI GTCTC 1 cut(s) 776
BstMBI GATC 2 cut(s) 64, 628
BstMWI GCNNNNNNNGC 1 cut(s) 668
BstNI CCWGG 1 cut(s) 542
BstSCI CCNGG 1 cut(s) 540
BstSLI GKGCMC 1 cut(s) 40
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 1 cut(s) 233
BstV2I GAAGAC 1 cut(s) 291
BstXI CCANNNNNNTGG 1 cut(s) 572
BsuRI GGCC 4 cut(s) 263, 323, 545, 795
BtsCI GGATG 2 cut(s) 176, 180
BtsIMutI CAGTG 2 cut(s) 463, 810
Cac8I GCNNGC 3 cut(s) 168, 265, 733
CfoI GCGC 1 cut(s) 120
Cfr10I RCCGGY 1 cut(s) 745
Cfr13I GGNCC 1 cut(s) 794
CspAI ACCGGT 1 cut(s) 745
CviAII CATG 3 cut(s) 268, 314, 319
DdeI CTNAG 7 cut(s) 108, 192, 241, 447, 520, 672, 688
DpnI GATC 2 cut(s) 66, 630
DpnII GATC 2 cut(s) 64, 628
DraIII CACNNNGTG 1 cut(s) 35
EaeI YGGCCR 2 cut(s) 261, 321
Eam1104I CTCTTC 1 cut(s) 498
EarI CTCTTC 1 cut(s) 498
Ecl136II GAGCTC 1 cut(s) 508
Eco130I CCWWGG 1 cut(s) 546
Eco24I GRGCYC 1 cut(s) 510
Eco53kI GAGCTC 1 cut(s) 508
Eco57I CTGAAG 1 cut(s) 630
Eco88I CYCGRG 2 cut(s) 171, 299
EcoICRI GAGCTC 1 cut(s) 508
EcoRII CCWGG 1 cut(s) 540
EcoT14I CCWWGG 1 cut(s) 546
EcoT22I ATGCAT 1 cut(s) 315
EcoT38I GRGCYC 1 cut(s) 510
ErhI CCWWGG 1 cut(s) 546
FaeI CATG 3 cut(s) 271, 317, 322
FalI AAGNNNNNCTT 4 cut(s) 201, 233, 474, 506
FatI CATG 3 cut(s) 267, 313, 318
FblI GTMKAC 1 cut(s) 352
Fnu4HI GCNGC 1 cut(s) 222
FokI GGATG 2 cut(s) 163, 167
FriOI GRGCYC 1 cut(s) 510
Fsp4HI GCNGC 1 cut(s) 222
FspBI CTAG 2 cut(s) 231, 429
GlaI GCGC 1 cut(s) 119
GluI GCNGC 1 cut(s) 222
HaeIII GGCC 4 cut(s) 263, 323, 545, 795
HapII CCGG 1 cut(s) 746
HhaI GCGC 1 cut(s) 120
Hin1II CATG 3 cut(s) 271, 317, 322
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HindIII AAGCTT 1 cut(s) 359
HinfI GANTC 6 cut(s) 122, 377, 472, 516, 560, 696
HpaII CCGG 1 cut(s) 746
HphI GGTGA 1 cut(s) 490
Hpy166II GTNNAC 2 cut(s) 38, 353
Hpy188I TCNGA 7 cut(s) 69, 109, 193, 244, 253, 391, 512
Hpy188III TCNNGA 3 cut(s) 231, 301, 440
Hpy8I GTNNAC 2 cut(s) 38, 353
HpyCH4III ACNGT 2 cut(s) 488, 805
HpyCH4V TGCA 6 cut(s) 38, 221, 313, 463, 638, 731
HpyF10VI GCNNNNNNNGC 1 cut(s) 668
HpyF3I CTNAG 7 cut(s) 108, 192, 241, 447, 520, 672, 688
Hsp92II CATG 3 cut(s) 271, 317, 322
HspAI GCGC 1 cut(s) 118
Kzo9I GATC 2 cut(s) 64, 628
LguI GCTCTTC 1 cut(s) 498
LmnI GCTCC 2 cut(s) 163, 513
Lsp1109I GCAGC 1 cut(s) 233
LweI GCATC 2 cut(s) 185, 300
MaeI CTAG 2 cut(s) 231, 429
MaeIII GTNAC 3 cut(s) 111, 294, 799
MalI GATC 2 cut(s) 66, 630
MboI GATC 2 cut(s) 64, 628
MboII GAAGA 4 cut(s) 221, 296, 378, 515
MfeI CAATTG 1 cut(s) 48
MhlI GDGCHC 2 cut(s) 40, 510
MlsI TGGCCA 1 cut(s) 263
MluCI AATT 6 cut(s) 48, 84, 152, 203, 338, 421
MluNI TGGCCA 1 cut(s) 263
MlyI GAGTC 3 cut(s) 131, 554, 690
MmeI TCCRAC 1 cut(s) 724
MnlI CCTC 7 cut(s) 154, 250, 403, 436, 506, 652, 667
Mox20I TGGCCA 1 cut(s) 263
Mph1103I ATGCAT 1 cut(s) 315
MscI TGGCCA 1 cut(s) 263
MseI TTAA 4 cut(s) 492, 530, 624, 777
MslI CAYNNNNRTG 2 cut(s) 570, 779
Msp20I TGGCCA 1 cut(s) 263
MspA1I CMGCKG 1 cut(s) 671
MspCI CTTAAG 1 cut(s) 491
MspI CCGG 1 cut(s) 746
MspR9I CCNGG 1 cut(s) 542
MunI CAATTG 1 cut(s) 48
MvaI CCWGG 1 cut(s) 542
MvnI CGCG 1 cut(s) 120
MwoI GCNNNNNNNGC 1 cut(s) 668
NdeII GATC 2 cut(s) 64, 628
NlaIII CATG 3 cut(s) 271, 317, 322
NmuCI GTSAC 1 cut(s) 799
NsiI ATGCAT 1 cut(s) 315
PaeR7I CTCGAG 2 cut(s) 171, 299
PciSI GCTCTTC 1 cut(s) 498
PfeI GAWTC 3 cut(s) 377, 472, 516
PinAI ACCGGT 1 cut(s) 745
PkrI GCNGC 1 cut(s) 223
PleI GAGTC 3 cut(s) 130, 554, 690
PpsI GAGTC 3 cut(s) 130, 554, 690
Psp124BI GAGCTC 1 cut(s) 510
Psp6I CCWGG 1 cut(s) 540
PspGI CCWGG 1 cut(s) 540
PspPI GGNCC 1 cut(s) 794
PspXI VCTCGAGB 1 cut(s) 171
PvuII CAGCTG 1 cut(s) 671
RseI CAYNNNNRTG 2 cut(s) 570, 779
SacI GAGCTC 1 cut(s) 510
SapI GCTCTTC 1 cut(s) 498
SaqAI TTAA 4 cut(s) 492, 530, 624, 777
SatI GCNGC 1 cut(s) 222
Sau3AI GATC 2 cut(s) 64, 628
Sau96I GGNCC 1 cut(s) 794
SchI GAGTC 3 cut(s) 131, 554, 690
ScrFI CCNGG 1 cut(s) 542
SduI GDGCHC 2 cut(s) 40, 510
SfaNI GCATC 2 cut(s) 185, 300
Sfr274I CTCGAG 2 cut(s) 171, 299
SlaI CTCGAG 2 cut(s) 171, 299
SmiMI CAYNNNNRTG 2 cut(s) 570, 779
SmlI CTYRAG 4 cut(s) 171, 299, 491, 711
SmoI CTYRAG 4 cut(s) 171, 299, 491, 711
Sse9I AATT 6 cut(s) 48, 84, 152, 203, 338, 421
SsiI CCGC 1 cut(s) 808
SspMI CTAG 2 cut(s) 231, 429
SstI GAGCTC 1 cut(s) 510
StyD4I CCNGG 1 cut(s) 540
StyI CCWWGG 1 cut(s) 546
TaaI ACNGT 2 cut(s) 488, 805
TaqI TCGA 3 cut(s) 172, 300, 558
TasI AATT 6 cut(s) 48, 84, 152, 203, 338, 421
TfiI GAWTC 3 cut(s) 377, 472, 516
Tru1I TTAA 4 cut(s) 492, 530, 624, 777
Tru9I TTAA 4 cut(s) 492, 530, 624, 777
TscAI CASTG 2 cut(s) 470, 810
TseFI GTSAC 1 cut(s) 799
TseI GCWGC 1 cut(s) 221
Tsp45I GTSAC 1 cut(s) 799
TspDTI ATGAA 4 cut(s) 35, 267, 516, 797
TspRI CASTG 2 cut(s) 470, 810
Vha464I CTTAAG 1 cut(s) 491
VneI GTGCAC 1 cut(s) 36
XbaI TCTAGA 1 cut(s) 230
XhoI CTCGAG 2 cut(s) 171, 299
XmiI GTMKAC 1 cut(s) 352
XspI CTAG 2 cut(s) 231, 429
Zsp2I ATGCAT 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.