Rh5AG438400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0004466
Physical Location & Seq
Reverse (-)
75291389 .. 75429015
137627 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG438400.1

Sequence Viewer

Length: 1179 bp
ATGGATTCCCGGCACGCATCTCTAGGCCGTCGAACGTTGGAAGAAATTCGTCAAAAGCGAGCTGCAGAAAGATTGAGCAAAACTTCTTCGGGGCCCGATCTCAGCCAGATCCCACCTATCGATAATGGAGGAATGAGAAAATCCGAGAGCGCAAATCGACTCTCTGAGAGCGATGTTAGTACTTTAGTTTCTCAGATAAGAGACTTGGATAGGAAGAATGCAGAACTGGAAGAACAAAACAAAAATTTGGCTTCCATGCTTGAAACAAAGGAAGCCGGGAGTAATGTGCTGCAGAAACGCTTGAATGATCTGGAGCAAAATACCGTACCGTCTTTGAGGAAAGCTCTCAAGGATGTCGCAATGGAGAAAGATGCAGCAGTTGTTGCACGGGAGGATCTCTCTGCCCAGCTGCGTATGCTCAAGAAACGTCTAAAGGATGCAGAAGAAGAACAGTATCGAGCTGAGGAAGATGCAGCATCCCTGAGAGCAGAACTCAATATGATACAGCAACAAACAATGAATGGCTCACTTGGTGCACTAAACTCAATTGCTAATCCACCTGATCAGATAAAAAAATTAGAAATTGAGTTAGCTAACATAAAGTCTGAGTTACAGCGCGAGTCAATGTTTAGACAGCAAGAACGACAACAATTAGGAGAGGAGCAGGCTCGAGCATCCATCCTTACATCTGAGAAGCAAGAATTGGAAGAAAAACTTGCAGCTTTGTCTAGAAAGTCCTCAGAAGTGTCAGACAAAGTGGCCAGCATGGGATTTTCATTAGAAGACAAGGAGGTACTCGAGAAGCAGATGCATGACATGGCTGTAGTGATTGAAAAATTGGAAAGCAGTAGACAAAAGCTTTTGATGGAGATTGATTCCCAATCTTCGGAGATAGAAAGGCTTTTTGAGGAAAACTCTAATTTATCTAGTTCTCATCAGGAGGCTATGAGCATAGCTTTGCACTGGGAGAATCAGGTGAAAGACTGTCTTAAGCAAAATGAAGAGCTCCGAGGAATCTTAGATAAGTTAAGAACTGACCAGGCCAAGGGGTTGCTCGACTTCCATAGCAATGGTTCTAACAAGACTGGTTTAGCTGAATACACACCTGAAGTTCTCTCTATTAAGGATCAACTTGCAAAAGAACAGAGCAAAGCAGAGGCATTATCAGCTGAGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

392

Amino Acids

44.13

Weight (kDa)

5.16

Isoelectric Point (pI)

57.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 850
AccII CGCG 1 cut(s) 618
AclI AACGTT 1 cut(s) 35
AclWI GGATC 3 cut(s) 103, 402, 1134
AcoI YGGCCR 1 cut(s) 759
AcsI RAATTY 2 cut(s) 45, 244
AcuI CTGAAG 1 cut(s) 1128
AdeI CACNNNGTG 1 cut(s) 533
AfaI GTAC 3 cut(s) 181, 327, 795
AfiI CCNNNNNNNGG 2 cut(s) 886, 1045
AflII CTTAAG 1 cut(s) 989
AgsI TTSAA 3 cut(s) 263, 304, 833
AjnI CCWGG 1 cut(s) 1038
AjuI GAANNNNNNNTTGG 2 cut(s) 686, 718
Alw21I GWGCWC 2 cut(s) 538, 1008
Alw26I GTCTC 1 cut(s) 195
Alw44I GTGCAC 1 cut(s) 534
AlwI GGATC 3 cut(s) 103, 402, 1134
Ama87I CYCGRG 2 cut(s) 669, 797
AoxI GGCC 4 cut(s) 25, 92, 759, 1041
ApaI GGGCCC 1 cut(s) 96
ApaLI GTGCAC 1 cut(s) 534
ApeKI GCWGC 6 cut(s) 62, 289, 374, 409, 473, 719
ApoI RAATTY 2 cut(s) 45, 244
Asp700I GAANNNNTTC 1 cut(s) 45
AspLEI GCGC 2 cut(s) 152, 618
AspS9I GGNCC 2 cut(s) 92, 93
AsuC2I CCSGG 2 cut(s) 10, 277
AsuHPI GGTGA 1 cut(s) 988
AvaI CYCGRG 2 cut(s) 669, 797
BaeGI GKGCMC 2 cut(s) 96, 538
BalI TGGCCA 1 cut(s) 761
BanII GRGCYC 2 cut(s) 96, 1008
BbsI GAAGAC 1 cut(s) 789
Bbv12I GWGCWC 2 cut(s) 538, 1008
BbvCI CCTCAGC 1 cut(s) 462
BbvI GCAGC 6 cut(s) 49, 276, 386, 396, 485, 731
BccI CCATC 2 cut(s) 686, 859
BceAI ACGGC 1 cut(s) 12
BciT130I CCWGG 1 cut(s) 1040
BclI TGATCA 1 cut(s) 562
BcnI CCSGG 2 cut(s) 10, 277
BcoDI GTCTC 1 cut(s) 195
BfaI CTAG 3 cut(s) 23, 729, 927
BfmI CTRYAG 3 cut(s) 63, 290, 822
BfrI CTTAAG 1 cut(s) 989
BisI GCNGC 6 cut(s) 63, 290, 375, 410, 474, 720
BlsI GCNGC 6 cut(s) 64, 291, 376, 411, 475, 721
BmcAI AGTACT 1 cut(s) 181
Bme1390I CCNGG 3 cut(s) 10, 277, 1040
BmeT110I CYCGRG 2 cut(s) 669, 797
BmgT120I GGNCC 2 cut(s) 92, 93
BmiI GGNNCC 2 cut(s) 93, 94
BmrFI CCNGG 3 cut(s) 10, 277, 1040
BmrI ACTGGG 1 cut(s) 973
BmsI GCATC 7 cut(s) 26, 361, 427, 460, 485, 683, 798
BmuI ACTGGG 1 cut(s) 973
BpiI GAAGAC 1 cut(s) 789
BplI GAGNNNNNCTC 8 cut(s) 328, 360, 383, 415, 477, 509, 899, 931
BpmI CTGGAG 1 cut(s) 332
Bpu10I CCTNAGC 1 cut(s) 462
BpuEI CTTGAG 2 cut(s) 332, 404
BpuMI CCSGG 2 cut(s) 10, 277
Bsa29I ATCGAT 1 cut(s) 120
BsaJI CCNNGG 2 cut(s) 1009, 1044
Bsc4I CCNNNNNNNGG 2 cut(s) 886, 1045
Bse1I ACTGG 3 cut(s) 231, 968, 1090
Bse3DI GCAATG 2 cut(s) 366, 1075
BseBI CCWGG 1 cut(s) 1040
BseCI ATCGAT 1 cut(s) 120
BseDI CCNNGG 2 cut(s) 1009, 1044
BseGI GGATG 5 cut(s) 358, 442, 476, 674, 678
BseLI CCNNNNNNNGG 2 cut(s) 886, 1045
BseMI GCAATG 2 cut(s) 366, 1075
BseMII CTCAG 9 cut(s) 115, 156, 206, 453, 473, 597, 681, 753, 1161
BseNI ACTGG 3 cut(s) 231, 968, 1090
BseRI GAGGAG 1 cut(s) 674
BseSI GKGCMC 2 cut(s) 96, 538
BseXI GCAGC 6 cut(s) 49, 276, 386, 396, 485, 731
BseYI CCCAGC 1 cut(s) 405
Bsh1236I CGCG 1 cut(s) 618
BshFI GGCC 4 cut(s) 27, 94, 761, 1043
BshVI ATCGAT 1 cut(s) 120
BsiHKAI GWGCWC 2 cut(s) 538, 1008
BsiHKCI CYCGRG 2 cut(s) 669, 797
BsiSI CCGG 2 cut(s) 10, 276
BslI CCNNNNNNNGG 2 cut(s) 886, 1045
BsmAI GTCTC 1 cut(s) 195
BsmI GAATGC 1 cut(s) 223
BsnI GGCC 4 cut(s) 27, 94, 761, 1043
BsoBI CYCGRG 2 cut(s) 669, 797
Bsp120I GGGCCC 1 cut(s) 92
Bsp1286I GDGCHC 3 cut(s) 96, 538, 1008
Bsp143I GATC 6 cut(s) 97, 108, 307, 394, 562, 1126
BspANI GGCC 4 cut(s) 27, 94, 761, 1043
BspCNI CTCAG 9 cut(s) 114, 157, 205, 454, 474, 598, 682, 752, 1162
BspDI ATCGAT 1 cut(s) 120
BspFNI CGCG 1 cut(s) 618
BspLI GGNNCC 2 cut(s) 93, 94
BspMAI CTGCAG 2 cut(s) 67, 294
BspPI GGATC 3 cut(s) 103, 402, 1134
BspQI GCTCTTC 1 cut(s) 996
BspTI CTTAAG 1 cut(s) 989
BsrDI GCAATG 2 cut(s) 366, 1075
BsrI ACTGG 3 cut(s) 231, 968, 1090
BssECI CCNNGG 2 cut(s) 1009, 1044
BssMI GATC 6 cut(s) 97, 108, 307, 394, 562, 1126
BssT1I CCWWGG 1 cut(s) 1044
Bst2UI CCWGG 1 cut(s) 1040
Bst4CI ACNGT 4 cut(s) 325, 330, 453, 986
Bst6I CTCTTC 1 cut(s) 996
BstAFI CTTAAG 1 cut(s) 989
BstAPI GCANNNNNTGC 1 cut(s) 383
BstC8I GCNNGC 4 cut(s) 15, 60, 666, 763
BstF5I GGATG 5 cut(s) 358, 442, 476, 674, 678
BstFNI CGCG 1 cut(s) 618
BstHHI GCGC 2 cut(s) 152, 618
BstKTI GATC 6 cut(s) 100, 111, 310, 397, 565, 1129
BstMAI GTCTC 1 cut(s) 195
BstMBI GATC 6 cut(s) 97, 108, 307, 394, 562, 1126
BstMWI GCNNNNNNNGC 3 cut(s) 383, 415, 1166
BstNI CCWGG 1 cut(s) 1040
BstSCI CCNGG 3 cut(s) 8, 275, 1038
BstSFI CTRYAG 3 cut(s) 63, 290, 822
BstSLI GKGCMC 2 cut(s) 96, 538
BstUI CGCG 1 cut(s) 618
BstV1I GCAGC 6 cut(s) 49, 276, 386, 396, 485, 731
BstV2I GAAGAC 1 cut(s) 789
BstX2I RGATCY 2 cut(s) 108, 394
BstXI CCANNNNNNTGG 1 cut(s) 1070
BstYI RGATCY 2 cut(s) 108, 394
Bsu15I ATCGAT 1 cut(s) 120
BsuRI GGCC 4 cut(s) 27, 94, 761, 1043
BsuTUI ATCGAT 1 cut(s) 120
BtgZI GCGATG 1 cut(s) 186
BtsCI GGATG 5 cut(s) 358, 442, 476, 674, 678
BtsIMutI CAGTG 1 cut(s) 961
Cac8I GCNNGC 4 cut(s) 15, 60, 666, 763
CfoI GCGC 2 cut(s) 152, 618
Cfr13I GGNCC 2 cut(s) 92, 93
ClaI ATCGAT 1 cut(s) 120
Csp6I GTAC 3 cut(s) 180, 326, 794
CviAII CATG 4 cut(s) 256, 766, 812, 817
CviQI GTAC 3 cut(s) 180, 326, 794
DpnI GATC 6 cut(s) 99, 110, 309, 396, 564, 1128
DpnII GATC 6 cut(s) 97, 108, 307, 394, 562, 1126
DraIII CACNNNGTG 1 cut(s) 533
EaeI YGGCCR 1 cut(s) 759
Eam1104I CTCTTC 1 cut(s) 996
EarI CTCTTC 1 cut(s) 996
Ecl136II GAGCTC 1 cut(s) 1006
Eco130I CCWWGG 1 cut(s) 1044
Eco24I GRGCYC 2 cut(s) 96, 1008
Eco53kI GAGCTC 1 cut(s) 1006
Eco57I CTGAAG 1 cut(s) 1128
Eco88I CYCGRG 2 cut(s) 669, 797
EcoICRI GAGCTC 1 cut(s) 1006
EcoO109I RGGNCCY 1 cut(s) 92
EcoRII CCWGG 1 cut(s) 1038
EcoT14I CCWWGG 1 cut(s) 1044
EcoT22I ATGCAT 1 cut(s) 813
EcoT38I GRGCYC 2 cut(s) 96, 1008
ErhI CCWWGG 1 cut(s) 1044
FaeI CATG 4 cut(s) 259, 769, 815, 820
FalI AAGNNNNNCTT 4 cut(s) 699, 731, 972, 1004
FatI CATG 4 cut(s) 255, 765, 811, 816
FbaI TGATCA 1 cut(s) 562
FblI GTMKAC 1 cut(s) 850
Fnu4HI GCNGC 6 cut(s) 63, 290, 375, 410, 474, 720
FokI GGATG 5 cut(s) 365, 449, 463, 661, 665
FriOI GRGCYC 2 cut(s) 96, 1008
Fsp4HI GCNGC 6 cut(s) 63, 290, 375, 410, 474, 720
FspBI CTAG 3 cut(s) 23, 729, 927
GlaI GCGC 2 cut(s) 151, 617
GluI GCNGC 6 cut(s) 63, 290, 375, 410, 474, 720
GsaI CCCAGC 1 cut(s) 409
GsuI CTGGAG 1 cut(s) 332
HaeIII GGCC 4 cut(s) 27, 94, 761, 1043
HapII CCGG 2 cut(s) 10, 276
HhaI GCGC 2 cut(s) 152, 618
Hin1II CATG 4 cut(s) 259, 769, 815, 820
Hin6I GCGC 2 cut(s) 150, 616
HinP1I GCGC 2 cut(s) 150, 616
HindIII AAGCTT 1 cut(s) 857
HinfI GANTC 6 cut(s) 5, 159, 620, 875, 970, 1014
HpaII CCGG 2 cut(s) 10, 276
HphI GGTGA 1 cut(s) 988
Hpy166II GTNNAC 2 cut(s) 536, 851
Hpy188III TCNNGA 5 cut(s) 311, 421, 729, 799, 938
Hpy8I GTNNAC 2 cut(s) 536, 851
Hpy99I CGWCG 1 cut(s) 33
HpyCH4III ACNGT 4 cut(s) 325, 330, 453, 986
HpyCH4IV ACGT 2 cut(s) 35, 427
HpyF10VI GCNNNNNNNGC 3 cut(s) 383, 415, 1166
HpySE526I ACGT 2 cut(s) 35, 427
Hsp92II CATG 4 cut(s) 259, 769, 815, 820
HspAI GCGC 2 cut(s) 150, 616
Ksp22I TGATCA 1 cut(s) 562
Kzo9I GATC 6 cut(s) 97, 108, 307, 394, 562, 1126
LguI GCTCTTC 1 cut(s) 996
LmnI GCTCC 3 cut(s) 313, 661, 1011
Lsp1109I GCAGC 6 cut(s) 49, 276, 386, 396, 485, 731
LweI GCATC 7 cut(s) 26, 361, 427, 460, 485, 683, 798
MaeI CTAG 3 cut(s) 23, 729, 927
MaeII ACGT 2 cut(s) 35, 427
MaeIII GTNAC 1 cut(s) 609
MalI GATC 6 cut(s) 99, 110, 309, 396, 564, 1128
MboI GATC 6 cut(s) 97, 108, 307, 394, 562, 1126
MfeI CAATTG 1 cut(s) 546
MflI RGATCY 2 cut(s) 108, 394
MhlI GDGCHC 3 cut(s) 96, 538, 1008
MlsI TGGCCA 1 cut(s) 761
MluNI TGGCCA 1 cut(s) 761
MlyI GAGTC 2 cut(s) 153, 629
MmeI TCCRAC 1 cut(s) 18
Mox20I TGGCCA 1 cut(s) 761
Mph1103I ATGCAT 1 cut(s) 813
MroXI GAANNNNTTC 1 cut(s) 45
MscI TGGCCA 1 cut(s) 761
MseI TTAA 4 cut(s) 990, 1028, 1122, 1177
MslI CAYNNNNRTG 1 cut(s) 1068
Msp20I TGGCCA 1 cut(s) 761
MspA1I CMGCKG 2 cut(s) 409, 1169
MspCI CTTAAG 1 cut(s) 989
MspI CCGG 2 cut(s) 10, 276
MspR9I CCNGG 3 cut(s) 10, 277, 1040
MunI CAATTG 1 cut(s) 546
Mva1269I GAATGC 1 cut(s) 223
MvaI CCWGG 1 cut(s) 1040
MvnI CGCG 1 cut(s) 618
MwoI GCNNNNNNNGC 3 cut(s) 383, 415, 1166
NciI CCSGG 2 cut(s) 10, 277
NdeII GATC 6 cut(s) 97, 108, 307, 394, 562, 1126
NlaIII CATG 4 cut(s) 259, 769, 815, 820
NlaIV GGNNCC 2 cut(s) 93, 94
NsiI ATGCAT 1 cut(s) 813
PaeR7I CTCGAG 2 cut(s) 669, 797
PciSI GCTCTTC 1 cut(s) 996
PcsI WCGNNNNNNNCGW 1 cut(s) 55
PctI GAATGC 1 cut(s) 223
PdmI GAANNNNTTC 1 cut(s) 45
PfeI GAWTC 4 cut(s) 5, 875, 970, 1014
PkrI GCNGC 6 cut(s) 64, 291, 376, 411, 475, 721
PleI GAGTC 2 cut(s) 153, 628
PpsI GAGTC 2 cut(s) 153, 628
Psp124BI GAGCTC 1 cut(s) 1008
Psp1406I AACGTT 1 cut(s) 35
Psp6I CCWGG 1 cut(s) 1038
PspFI CCCAGC 1 cut(s) 405
PspGI CCWGG 1 cut(s) 1038
PspN4I GGNNCC 2 cut(s) 93, 94
PspOMI GGGCCC 1 cut(s) 92
PspPI GGNCC 2 cut(s) 92, 93
PspXI VCTCGAGB 1 cut(s) 669
PstI CTGCAG 2 cut(s) 67, 294
PsuI RGATCY 2 cut(s) 108, 394
PvuII CAGCTG 2 cut(s) 409, 1169
RsaI GTAC 3 cut(s) 181, 327, 795
RsaNI GTAC 3 cut(s) 180, 326, 794
RseI CAYNNNNRTG 1 cut(s) 1068
SacI GAGCTC 1 cut(s) 1008
SapI GCTCTTC 1 cut(s) 996
SaqAI TTAA 4 cut(s) 990, 1028, 1122, 1177
SatI GCNGC 6 cut(s) 63, 290, 375, 410, 474, 720
Sau3AI GATC 6 cut(s) 97, 108, 307, 394, 562, 1126
Sau96I GGNCC 2 cut(s) 92, 93
ScaI AGTACT 1 cut(s) 181
SchI GAGTC 2 cut(s) 153, 629
ScrFI CCNGG 3 cut(s) 10, 277, 1040
SduI GDGCHC 3 cut(s) 96, 538, 1008
SfaNI GCATC 7 cut(s) 26, 361, 427, 460, 485, 683, 798
SfcI CTRYAG 3 cut(s) 63, 290, 822
Sfr274I CTCGAG 2 cut(s) 669, 797
SlaI CTCGAG 2 cut(s) 669, 797
SmiMI CAYNNNNRTG 1 cut(s) 1068
SmlI CTYRAG 5 cut(s) 347, 419, 669, 797, 989
SmoI CTYRAG 5 cut(s) 347, 419, 669, 797, 989
SspMI CTAG 3 cut(s) 23, 729, 927
SstI GAGCTC 1 cut(s) 1008
StyD4I CCNGG 3 cut(s) 8, 275, 1038
StyI CCWWGG 1 cut(s) 1044
TaaI ACNGT 4 cut(s) 325, 330, 453, 986
TaiI ACGT 2 cut(s) 38, 430
TaqI TCGA 7 cut(s) 31, 120, 157, 457, 670, 798, 1056
TatI WGTACW 1 cut(s) 179
TfiI GAWTC 4 cut(s) 5, 875, 970, 1014
Tru1I TTAA 4 cut(s) 990, 1028, 1122, 1177
Tru9I TTAA 4 cut(s) 990, 1028, 1122, 1177
TscAI CASTG 1 cut(s) 968
TseI GCWGC 6 cut(s) 62, 289, 374, 409, 473, 719
TspDTI ATGAA 3 cut(s) 533, 765, 1014
TspRI CASTG 1 cut(s) 968
Vha464I CTTAAG 1 cut(s) 989
VneI GTGCAC 1 cut(s) 534
XapI RAATTY 2 cut(s) 45, 244
XbaI TCTAGA 1 cut(s) 728
XhoI CTCGAG 2 cut(s) 669, 797
XmiI GTMKAC 1 cut(s) 850
XmnI GAANNNNTTC 1 cut(s) 45
XspI CTAG 3 cut(s) 23, 729, 927
ZrmI AGTACT 1 cut(s) 181
Zsp2I ATGCAT 1 cut(s) 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.